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Molecular recognition detected by electrochemiluminescence (ECL): A method for studying gene amplification
MOLECULAR
RECOGNITION
275
ATPASE ACTIVITY IN POLYAMMONIUM MACROCYCLES: THE INFLUENCE OF RING SIZE AND HETEROATOMS. M. E Mertes, K. B. Mertes, P. of Medicinal Chemistry and Paoletti, and A. Bianchi. Departments Chemistry, University of Kansas, Lawrence, KS 66645 USA and Department of Chemistry, University of Florence, I-50144 Florence, Italy. Polyammonium macrocycles have been observed to show ATPase as well as kinase-related activity. A series of polyammonium macrocycles ranging in size from [211N7 to t361~~~ and including several mixed aza-oxo-macrocycleshave now been examined to ascertain the importance of ring size and hydrogen-bonding interactions on ATPase activity. the potential role of a variety of metal ions in Furthermore, regulation of activity has also been probed.
1009
1010
MOLECULAR RECOGNITION DETECTEDBYELECTR0XEMILUMINESCENCE (ECL): A METHOD FOR STUDYING GENE AMPLIFICATION. J. Kenten, L. Hall, H.
Shah, J. Link, D. Lupold, D. Iacuzio, M. Farrell*, M. Powell, G. Lowke & R. Massey. IGEN Inc., 1530 E. Jefferson St., Rockville, MD 20852, *Georgetown University Hospital, Washington, DC 20007 USA. A system has been developed for using ECL to study pycleic acid hybridization. Oligonucleotideprobes labeled with Ru(bpy) wereused to detect & quantify DNA arq?lification by the polyrnerasec;la, in reaction (PCR). Hm papillama virus (types 16 & 18) could be rapidly det ted in cell lines based on PCR followed by n-easuringECL in the ORI& -1 Analyzer. Labeled oligonucleotideshave also been used to detect point mutations in the Ha-ras oncogene based on similar methods. A major advantage of ECL is the ability to rneasurehybridization quantitatively in a homogeneous (non-separation)format.
1ol.l
MOLECULAR RECOGNITICNDE~DBY
FXJXTIiOCHEMILUMIIW3XNCE
(ECL).
H. Shah, L. Hall, J. Leland, R. Kamin, G. Zoski, R. Sugasawara, G. Dale, R. Titn-as,M. Powell, G. Lowke & R. Massey. IGEN Inc., 1530 E. Jefferson St., Rockville, MD 20852 USA. The use of electroc~luninescent metal chelates for studying nucleic acid hybridization & n-antibody interactions has been (ORI with a flow-c@1 in which electrodes evaluated. An instant were placed was developed for this work. Ru(bpy13 covalently bound to a xanthine derivative was mixed with specific monoclonal antibodies & ECLwasthenmeasured. Achange in the photon fluxwas observedwhich was proportionaltothe amount of antibodyadded. Sir@arresults~~~e observed when an oligonucleotidecoupled to Ru(bpyj3 hybridized to a canplenentary DNA strand. Modulation in ECL provides a way to format a variety of quantitative hcsnogeneous(non-separation)assays.
RECOGNITION
275
ATPASE ACTIVITY IN POLYAMMONIUM MACROCYCLES: THE INFLUENCE OF RING SIZE AND HETEROATOMS. M. E Mertes, K. B. Mertes, P. of Medicinal Chemistry and Paoletti, and A. Bianchi. Departments Chemistry, University of Kansas, Lawrence, KS 66645 USA and Department of Chemistry, University of Florence, I-50144 Florence, Italy. Polyammonium macrocycles have been observed to show ATPase as well as kinase-related activity. A series of polyammonium macrocycles ranging in size from [211N7 to t361~~~ and including several mixed aza-oxo-macrocycleshave now been examined to ascertain the importance of ring size and hydrogen-bonding interactions on ATPase activity. the potential role of a variety of metal ions in Furthermore, regulation of activity has also been probed.
1009
1010
MOLECULAR RECOGNITION DETECTEDBYELECTR0XEMILUMINESCENCE (ECL): A METHOD FOR STUDYING GENE AMPLIFICATION. J. Kenten, L. Hall, H.
Shah, J. Link, D. Lupold, D. Iacuzio, M. Farrell*, M. Powell, G. Lowke & R. Massey. IGEN Inc., 1530 E. Jefferson St., Rockville, MD 20852, *Georgetown University Hospital, Washington, DC 20007 USA. A system has been developed for using ECL to study pycleic acid hybridization. Oligonucleotideprobes labeled with Ru(bpy) wereused to detect & quantify DNA arq?lification by the polyrnerasec;la, in reaction (PCR). Hm papillama virus (types 16 & 18) could be rapidly det ted in cell lines based on PCR followed by n-easuringECL in the ORI& -1 Analyzer. Labeled oligonucleotideshave also been used to detect point mutations in the Ha-ras oncogene based on similar methods. A major advantage of ECL is the ability to rneasurehybridization quantitatively in a homogeneous (non-separation)format.
1ol.l
MOLECULAR RECOGNITICNDE~DBY
FXJXTIiOCHEMILUMIIW3XNCE
(ECL).
H. Shah, L. Hall, J. Leland, R. Kamin, G. Zoski, R. Sugasawara, G. Dale, R. Titn-as,M. Powell, G. Lowke & R. Massey. IGEN Inc., 1530 E. Jefferson St., Rockville, MD 20852 USA. The use of electroc~luninescent metal chelates for studying nucleic acid hybridization & n-antibody interactions has been (ORI with a flow-c@1 in which electrodes evaluated. An instant were placed was developed for this work. Ru(bpy13 covalently bound to a xanthine derivative was mixed with specific monoclonal antibodies & ECLwasthenmeasured. Achange in the photon fluxwas observedwhich was proportionaltothe amount of antibodyadded. Sir@arresults~~~e observed when an oligonucleotidecoupled to Ru(bpyj3 hybridized to a canplenentary DNA strand. Modulation in ECL provides a way to format a variety of quantitative hcsnogeneous(non-separation)assays.