- Email: [email protected]
Morbidity and mortality in elderly with low total serum cholesterol
Zadorin
with PAD. We measured bra&al-ankle PWV (baPWV) as lower limb PWV by an automatic device in 101 healthy control subjects and 102 type 2 diabetic patients including 9 with PAD as defined by reduced ankle-brachial pressure index (ABI) less than 0.9. Diabetic patients without PAD showed a higher baPWV than the healthy control subjects. There was no difference in baPWV between the right and left legs in these groups. In contrast, among diabetic patients with PAD, baPWV was significantly lower in the affected legs as compared with non-affected legs, and the reduction in baPWV was greater in those with lower ABI. Both baPWV and ABI were increased following percutaneous transluminal angioplasty. These results indicate that lower-limb PWV is decreased in the affected legs in diabetic patients with PAD. Difference in lower-limb PWV between both legs may be indicative of presence of PAD.
I804 ENHANCED
EXPRESSION OF LEUKOTACTIN (Lkn-1) mRNA AND PROTEIN IN HUMAN THP-1 MONOCYTES-DERIVED FOAM CELLS AND IN ATHEROSCLEROTIC LESION
R. Yu’ C.-S. Kim’, T. Kawada’, B.-S. Kwon’ 'Uniuersify ofUlsan, -a Ulsan, South Korea: ‘Kyoto Uniuersify Kyoto, Japan Leukotactin-1 (Lkn-1), a new member of the CC chemokine family is involved in leukocyte trafficking and considered to be associated with inflammatory diseases. Recently we demonstrated that the mouse Lkn-1 (MRP-2) expression in macrophages is upregulated by oxLDL treatment in vitro and a high fat and cholesterol diet in viva, suggesting that Lkn-1 may be implicated in atherosclerosis. To explore the possible association of Lkn-1 in human atherosclerosis, we investigated Lkn-1 expression in human monocyte-derived foam cells and in atherosclerotic lesion. Human monocytic cells (THP-1) were differentiated into macrophage in vitro by PMA, and followed by incubation with oxLDL for 4 days. Foam cell formation was confirmed by Oil red-0 staining. Lkn-1 expression at the mRNA/protein level was measured by RT-PCIUELISA. The expression of Lkn-1 at mRNA level and the Lkn-1 release were markedly increased by oxLDL in both human THP-1 monocytes and the monocytes-derived foam cells in vitro. There was a significant increase in the Lkn-1 expression at mRNA level in atherosclerosis lesion and the protein level in plasma of the patients diagnosed with atherosclerosis. Our data suggest that Lkn-1 may play an important role in the development of atherosclerosis in human.
I805 FIVE YEAR IN RURAL
PREVALENCE MALAYSIA
IN CORONARY
RISK FACTORS
K. Yusoff’ , S. Singh’, M.D. Sulaiman’ , A. Pit’, H. Yunus’ , H. Nawawi’. ‘Faculty ofMedicine, Uniuersiti Kebangsaan Malaysia: ‘Department of Medicine and Pathology, Faculty ofMedicine, Uniuersiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia Ischaemic heart disease (IHD) is increasing in developing countries such as Malaysia. Whilst city-dwellers tend to be affected, IHD is starting to involve the rural population. This community-based cross-sectional study was to determine the trend in the prevalence of coronary risk factors (CRF) from 1993 to 1998 in rural Malaysia. In 1993 and 1998, demographic data and fasting venous bloods for lipid and sugar were taken from 692 and 726 adults respectively in one rural district. Among males, age-adjusted prevalence for hypertension (26.2% vs 30.6% for 1993 and 1998, p>O.O5) was unchanged. Prevalence of hyperlipidaemia (total cholesterol, T05.2 mmol/L: 17.8% vs 75.5%, piO.05; LDL>3.4mmol/L: 21.9% vs 64.9%, piO.05; triglyceride>l.7 mmol/L: 39.2% vs 55.2%), and overweight (17.7% vs 30.9%, piO.05) had increased but that of diabetes (4.4% vs 2.81%; pO.O5) and diabetes (3.5% vs 2.8%, p>O.O5) prevalence was unchanged while that of TC (23.5% vs 61.6%, piO.O5), LDL (26.1% vs 51.9%, piO.05) and triglyceride (27.9% vs 37.5%, p
I806 MORBIDITY TOTAL
AND MORTALITY SERUM CHOLESTEROL
A.K. Zacharof, C. Petrogiannopoulos, Red Cross Hospital, Athens, Greece Purpose
IN ELDERLY
WITH
C. Flevaris, G. Karachalios.
241 LOW
Hellenic
of the Study:
It is well known that low serum cholesterol has been with morbidity and mortality in the elderly. Methods: We analysed and studied our Department medical records of patients with hypolipidemia in focus of a) disease history, b) any disability, c) health habits, and d) cognitive function. The data were collected from 1986 to 1999. We analysed blood samples for total serum cholesterol, triglycerides and HDL, LDL values. Results: Of the 3,874 participants, 109 (2.8%) had total cholesterol levels < 150 mg/dl. Seventy-five percent of the low cholesterol group were male compared to 44% in the main study population. The low cholesterol group had significantly greater smoking history, current cigarettes smoked, diabetes history, angina and COPD symptoms, and assistance needed for heavy and light work. Men in the low cholesterol group had significantly lower blood pressure. After two years, 14 (12.8%) of the low cholesterol group had died vs 16 (7.3%) in the control group. There was no relationship to specific causes of death and cholesterol level. Conclusions: A very low cholesterol level in older individuals should be evaluated carefully to determine whether it is due to genetic or life-style factors such as diet or, more likely, is a marker of disease. associated
I807 THE STUDY
OF FAT-ACIDS COMPOSITION, OXlDATIVE STRESS AND ANTIOXIDANT ACTIVITY IN BLOOD OF PATIENTS WITH CARDIOVASCULAR DISEASES
E.M. Zadorin’,‘. ‘Cardiology Faculty, National Medical Uniuersify of Ukraine: 2Biochemistry and Pharmaceutical Faculty, National Medical Uniuersify ofUkraine, Kiev, Ukraine Objective:
Studing of lipid peroxidation processes and antioxidant activity in blood of healthy people and patients with cardio-vascular diseases is actual problem of practical and experimental medicine, that will allow to improve diagnostics and treatment of the given diseases. Design and Methods: In this research work was investigation there are lipid peroxidation processes, fat acids structure of lipids, and antioxidant activity in blood of patients with cardiovascular diseases and control persons with the help of a technique of definition MDA in plasma and erythrocytes of blood. High blood pressure in Pulmonary Artery an System Blood Circulation leading to Oxydative Stress in blood circulation. Primary, or unexplained, pulmonary hypertension (PPH) is a rare lung disorder in with the blood pressure in the pulmonary artery rises far above normal levels for no apparent reason. The pulmonary artery is the blood vessel carrying oxygen-poor blood from the right ventricle, one of the pumping chambers of the heart, to the lungs. In the lungs, the blood picks up oxygen and then flows to the left side of the heart, where it is pumped by the left ventricle to the rest of the body through the aorta. On the basis of clinical, biochemical and instrumental methods of investigation was ECG, Doppler EchoECG, luminescence spectrometry, gas chromatography etc. Results: The material for investigation was blood of 26 patients with Primary Pulmonary Hypertension, 45 with Arterial Hypertension, 24 with Congenital heart diseases which, were treated in the Central City Clinic of Ukraine. On the basis of clinic and instrumental methods of investigation was ECG, echocardiography etc. Systolic pressure in Pulmonary Artery according to Doppler information at trycuspidic circulation was 65,4&37,4 Controls were 13 healthy people. Lipid peroxidation (mmol MDA/l) in plasma of blood of patients with NYHA I-IV was: ascorbate-dependent peroxide oxidation (ADP) NYHA I-II 3.59&0.26, not initiation peroxide oxidation (NI) NYHA I-II 3.79&0.33; ADP NYHA III-IV 4.93&0.25, NI NYHA III-IV 4.12&0.53 lipid peroxydation in erythrocytes of blood of patients withNYHA I-IV was: NYHA I-II NI 3.27&0.21 mmol MDA/l, ADP mmol MDA/l 4.37&0.33, NADPH-depending peroxidation of lipids (NDP) 4.45&0.29; NYHA III-IV NI 3.60&0.22, ADP mmol MDA/l 4.46&0.24, NDP mmol MDA/l 6.13&0.37; Antioxidant activity in blood of patients is significantly reduced. Antioxidant activity (%) in blood of patients was: NYHA I-II 61.7&1.90, NYHA III-IV 57.9&1.7. Also, in the given work results gas chromatography the analysis at studying fat acids structure of lipids are submitted to blood (plasmas and erythrocytes) 14 patients with Primary Pulmonary hypertension (PPH), age of 15-35 years. The received results have allowed to establish, that for plasma of patients with PPH control authentically reduced contents of linolenic fat acid up to 18.6% is distinct from at 29.1% in the control, and also the increased level arahidonic acids
73rd EAS Congress
with PAD. We measured bra&al-ankle PWV (baPWV) as lower limb PWV by an automatic device in 101 healthy control subjects and 102 type 2 diabetic patients including 9 with PAD as defined by reduced ankle-brachial pressure index (ABI) less than 0.9. Diabetic patients without PAD showed a higher baPWV than the healthy control subjects. There was no difference in baPWV between the right and left legs in these groups. In contrast, among diabetic patients with PAD, baPWV was significantly lower in the affected legs as compared with non-affected legs, and the reduction in baPWV was greater in those with lower ABI. Both baPWV and ABI were increased following percutaneous transluminal angioplasty. These results indicate that lower-limb PWV is decreased in the affected legs in diabetic patients with PAD. Difference in lower-limb PWV between both legs may be indicative of presence of PAD.
I804 ENHANCED
EXPRESSION OF LEUKOTACTIN (Lkn-1) mRNA AND PROTEIN IN HUMAN THP-1 MONOCYTES-DERIVED FOAM CELLS AND IN ATHEROSCLEROTIC LESION
R. Yu’ C.-S. Kim’, T. Kawada’, B.-S. Kwon’ 'Uniuersify ofUlsan, -a Ulsan, South Korea: ‘Kyoto Uniuersify Kyoto, Japan Leukotactin-1 (Lkn-1), a new member of the CC chemokine family is involved in leukocyte trafficking and considered to be associated with inflammatory diseases. Recently we demonstrated that the mouse Lkn-1 (MRP-2) expression in macrophages is upregulated by oxLDL treatment in vitro and a high fat and cholesterol diet in viva, suggesting that Lkn-1 may be implicated in atherosclerosis. To explore the possible association of Lkn-1 in human atherosclerosis, we investigated Lkn-1 expression in human monocyte-derived foam cells and in atherosclerotic lesion. Human monocytic cells (THP-1) were differentiated into macrophage in vitro by PMA, and followed by incubation with oxLDL for 4 days. Foam cell formation was confirmed by Oil red-0 staining. Lkn-1 expression at the mRNA/protein level was measured by RT-PCIUELISA. The expression of Lkn-1 at mRNA level and the Lkn-1 release were markedly increased by oxLDL in both human THP-1 monocytes and the monocytes-derived foam cells in vitro. There was a significant increase in the Lkn-1 expression at mRNA level in atherosclerosis lesion and the protein level in plasma of the patients diagnosed with atherosclerosis. Our data suggest that Lkn-1 may play an important role in the development of atherosclerosis in human.
I805 FIVE YEAR IN RURAL
PREVALENCE MALAYSIA
IN CORONARY
RISK FACTORS
K. Yusoff’ , S. Singh’, M.D. Sulaiman’ , A. Pit’, H. Yunus’ , H. Nawawi’. ‘Faculty ofMedicine, Uniuersiti Kebangsaan Malaysia: ‘Department of Medicine and Pathology, Faculty ofMedicine, Uniuersiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia Ischaemic heart disease (IHD) is increasing in developing countries such as Malaysia. Whilst city-dwellers tend to be affected, IHD is starting to involve the rural population. This community-based cross-sectional study was to determine the trend in the prevalence of coronary risk factors (CRF) from 1993 to 1998 in rural Malaysia. In 1993 and 1998, demographic data and fasting venous bloods for lipid and sugar were taken from 692 and 726 adults respectively in one rural district. Among males, age-adjusted prevalence for hypertension (26.2% vs 30.6% for 1993 and 1998, p>O.O5) was unchanged. Prevalence of hyperlipidaemia (total cholesterol, T05.2 mmol/L: 17.8% vs 75.5%, piO.05; LDL>3.4mmol/L: 21.9% vs 64.9%, piO.05; triglyceride>l.7 mmol/L: 39.2% vs 55.2%), and overweight (17.7% vs 30.9%, piO.05) had increased but that of diabetes (4.4% vs 2.81%; p
I806 MORBIDITY TOTAL
AND MORTALITY SERUM CHOLESTEROL
A.K. Zacharof, C. Petrogiannopoulos, Red Cross Hospital, Athens, Greece Purpose
IN ELDERLY
WITH
C. Flevaris, G. Karachalios.
241 LOW
Hellenic
of the Study:
It is well known that low serum cholesterol has been with morbidity and mortality in the elderly. Methods: We analysed and studied our Department medical records of patients with hypolipidemia in focus of a) disease history, b) any disability, c) health habits, and d) cognitive function. The data were collected from 1986 to 1999. We analysed blood samples for total serum cholesterol, triglycerides and HDL, LDL values. Results: Of the 3,874 participants, 109 (2.8%) had total cholesterol levels < 150 mg/dl. Seventy-five percent of the low cholesterol group were male compared to 44% in the main study population. The low cholesterol group had significantly greater smoking history, current cigarettes smoked, diabetes history, angina and COPD symptoms, and assistance needed for heavy and light work. Men in the low cholesterol group had significantly lower blood pressure. After two years, 14 (12.8%) of the low cholesterol group had died vs 16 (7.3%) in the control group. There was no relationship to specific causes of death and cholesterol level. Conclusions: A very low cholesterol level in older individuals should be evaluated carefully to determine whether it is due to genetic or life-style factors such as diet or, more likely, is a marker of disease. associated
I807 THE STUDY
OF FAT-ACIDS COMPOSITION, OXlDATIVE STRESS AND ANTIOXIDANT ACTIVITY IN BLOOD OF PATIENTS WITH CARDIOVASCULAR DISEASES
E.M. Zadorin’,‘. ‘Cardiology Faculty, National Medical Uniuersify of Ukraine: 2Biochemistry and Pharmaceutical Faculty, National Medical Uniuersify ofUkraine, Kiev, Ukraine Objective:
Studing of lipid peroxidation processes and antioxidant activity in blood of healthy people and patients with cardio-vascular diseases is actual problem of practical and experimental medicine, that will allow to improve diagnostics and treatment of the given diseases. Design and Methods: In this research work was investigation there are lipid peroxidation processes, fat acids structure of lipids, and antioxidant activity in blood of patients with cardiovascular diseases and control persons with the help of a technique of definition MDA in plasma and erythrocytes of blood. High blood pressure in Pulmonary Artery an System Blood Circulation leading to Oxydative Stress in blood circulation. Primary, or unexplained, pulmonary hypertension (PPH) is a rare lung disorder in with the blood pressure in the pulmonary artery rises far above normal levels for no apparent reason. The pulmonary artery is the blood vessel carrying oxygen-poor blood from the right ventricle, one of the pumping chambers of the heart, to the lungs. In the lungs, the blood picks up oxygen and then flows to the left side of the heart, where it is pumped by the left ventricle to the rest of the body through the aorta. On the basis of clinical, biochemical and instrumental methods of investigation was ECG, Doppler EchoECG, luminescence spectrometry, gas chromatography etc. Results: The material for investigation was blood of 26 patients with Primary Pulmonary Hypertension, 45 with Arterial Hypertension, 24 with Congenital heart diseases which, were treated in the Central City Clinic of Ukraine. On the basis of clinic and instrumental methods of investigation was ECG, echocardiography etc. Systolic pressure in Pulmonary Artery according to Doppler information at trycuspidic circulation was 65,4&37,4 Controls were 13 healthy people. Lipid peroxidation (mmol MDA/l) in plasma of blood of patients with NYHA I-IV was: ascorbate-dependent peroxide oxidation (ADP) NYHA I-II 3.59&0.26, not initiation peroxide oxidation (NI) NYHA I-II 3.79&0.33; ADP NYHA III-IV 4.93&0.25, NI NYHA III-IV 4.12&0.53 lipid peroxydation in erythrocytes of blood of patients withNYHA I-IV was: NYHA I-II NI 3.27&0.21 mmol MDA/l, ADP mmol MDA/l 4.37&0.33, NADPH-depending peroxidation of lipids (NDP) 4.45&0.29; NYHA III-IV NI 3.60&0.22, ADP mmol MDA/l 4.46&0.24, NDP mmol MDA/l 6.13&0.37; Antioxidant activity in blood of patients is significantly reduced. Antioxidant activity (%) in blood of patients was: NYHA I-II 61.7&1.90, NYHA III-IV 57.9&1.7. Also, in the given work results gas chromatography the analysis at studying fat acids structure of lipids are submitted to blood (plasmas and erythrocytes) 14 patients with Primary Pulmonary hypertension (PPH), age of 15-35 years. The received results have allowed to establish, that for plasma of patients with PPH control authentically reduced contents of linolenic fat acid up to 18.6% is distinct from at 29.1% in the control, and also the increased level arahidonic acids
73rd EAS Congress