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Morphometric Analysis of Retinal Vascular Endothelial Cells in Rats
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Jpn J Ophthalmol Vol 46: 230–236, 2002
secondary ERMs (all eyes with complete PVD) were immunohistochemically studied. We used type I, II, III, IV collagen and fibronectin to study extracellular components, and glial fibrillary acidic protein (GFAP), S 100 protein, vimentin, and so forth to study cellular components. Results: All the specimens of idiopathic ERMs had the major components of the lamellar stained by type II collagen antibody, and one of 3 specimens of secondary ERMs had a minor component stained by type II collagen antibody. Compared with idiopathic ERMs with complete PVD, 2 of 3 specimens of idiopathic ERMs with partial PVD or no PVD contained rather thick collagen lamellar. Conclusion: There were differences between specimens of idiopathic ERMs and specimens of secondary ERMs in staining by type II collagen antibody, supposed by vitreous, in this study. Idiopathic ERM with attached posterior vitreous membrane may cause growth of collagen. (J Jpn Ophthalmol Soc 105:673– 681, 2001) Reiko Kinouchi,* Hiroyuki Hirokawa*,†, Naoyuki Miyokawa‡, Takeshi Nomiyama§, Taiichi Hikichi* and Akitoshi Yoshida* *Department of Ophthalmology, Asahikawa Medical College; †Department of Medical Informatics, Asahikawa Medical College Hospital; ‡Laboratory of Pathology, Asahikawa Medical College Hospital; §Department of Ophthalmology, Kitami Red Cross Hospital PII S0021-5155(01)00514-7
Morphometric Analysis of Retinal Vascular Endothelial Cells in Rats Introduction: In the present study, we observed the transformation of endothelial cells in the retinal vessels of normal rats and examined the relationship between morphometrical parameters and vessel calibers. Materials and Methods: Retinal vessels of male Wistar-Kyoto rats were stained with an en face silver staining method, and the vessel caliber, the axis of the cell oriented parallel to the longitudinal axis and to the transverse plane of the vessel, and the deviation angle of endothelial cells against the vessel axis were measured under a light microscope. Results: As the vessel caliber increased, endothelial cells showed a tendency to extend along the longitudinal axis in the arterioles, but endothelial cells remained unchanged in the venules. The deviation angle of endothelial cells was nearly parallel to the vessel axis especially in the arterioles.
Conclusions: Change in shape of endothelial cells in the retinal arterioles suggested a high shear stress in the large arterioles, and lower shear stress in the smaller arterioles with a decrease in caliber. In the venules, however, the cell shape was unchanged, and this suggests that the blood flow is fairly steady. (J Jpn Ophthalmol Soc 105:682–686, 2001) Tomoko Tsuruya, Satoru Kimura and Mitsuru Nakazawa Department of Ophthalmology, Hirosaki University PII S0021-5155(01)00515-9
Retinal Hazard from Blue Light Emitting Diode Purpose: To compare the effect of exposure time from a blue (460 nm) light emitting diode (LED) on the morphology of the outer retina and determine conditions where damage occurs. Materials and Methods: Young adult rhesus monkeys were anesthetized, and received blue LED exposure from a modified slit-lamp. A 3 mm beam of 0.85 mW was imaged onto the retina through a lens positioned before the cornea and exposure damage was determined at time intervals of 12 to 90 min. Fundus photography, fluorescein angiography (FAG), retinal tomography (HRT), and s-cone electroretinograms (S-ERG) were recorded at baseline, 2, and 30 days. Results: Two days after 40 min exposure, there was a grey, discolored region, which was overfluorescent in FAG, and an increase in HRT and S-ERG corresponding to the site which was exposed to LED light. In histological examination at 30 days, the LED had caused a marked disruption of the disks of photoreceptor cells, damaged retinal pigment epithelium (RPE) apical villi, and resulted in a loss of RPE melanin after 90 min exposure. Conclusion: A threshold level was found around 40 min. This morphological damage may impair function, and continuous exposure to blue light is potentially dangerous to vision. (J Jpn Ophthalmol Soc 105:687– 695, 2001) Ryouhei Koide*, Takako N Ueda†, William W Dawson‡, GM Hope‡, Ann Ellis§, Don Samuelson§, Toshihiko Ueda*, Shigehiro Iwabuchi*, Shohei Fukuda*, Miou Matsuishi*, Hajime Yasuhara*, Tetsuma Ozawa* and Donald Armstrong*§ *Department of Ophthalmology, School of Medicine, Showa University; †Department of Pharmacology, School of Medicine, Showa University; ‡Department of Ophthalmology, College of Medicine, University of
Jpn J Ophthalmol Vol 46: 230–236, 2002
secondary ERMs (all eyes with complete PVD) were immunohistochemically studied. We used type I, II, III, IV collagen and fibronectin to study extracellular components, and glial fibrillary acidic protein (GFAP), S 100 protein, vimentin, and so forth to study cellular components. Results: All the specimens of idiopathic ERMs had the major components of the lamellar stained by type II collagen antibody, and one of 3 specimens of secondary ERMs had a minor component stained by type II collagen antibody. Compared with idiopathic ERMs with complete PVD, 2 of 3 specimens of idiopathic ERMs with partial PVD or no PVD contained rather thick collagen lamellar. Conclusion: There were differences between specimens of idiopathic ERMs and specimens of secondary ERMs in staining by type II collagen antibody, supposed by vitreous, in this study. Idiopathic ERM with attached posterior vitreous membrane may cause growth of collagen. (J Jpn Ophthalmol Soc 105:673– 681, 2001) Reiko Kinouchi,* Hiroyuki Hirokawa*,†, Naoyuki Miyokawa‡, Takeshi Nomiyama§, Taiichi Hikichi* and Akitoshi Yoshida* *Department of Ophthalmology, Asahikawa Medical College; †Department of Medical Informatics, Asahikawa Medical College Hospital; ‡Laboratory of Pathology, Asahikawa Medical College Hospital; §Department of Ophthalmology, Kitami Red Cross Hospital PII S0021-5155(01)00514-7
Morphometric Analysis of Retinal Vascular Endothelial Cells in Rats Introduction: In the present study, we observed the transformation of endothelial cells in the retinal vessels of normal rats and examined the relationship between morphometrical parameters and vessel calibers. Materials and Methods: Retinal vessels of male Wistar-Kyoto rats were stained with an en face silver staining method, and the vessel caliber, the axis of the cell oriented parallel to the longitudinal axis and to the transverse plane of the vessel, and the deviation angle of endothelial cells against the vessel axis were measured under a light microscope. Results: As the vessel caliber increased, endothelial cells showed a tendency to extend along the longitudinal axis in the arterioles, but endothelial cells remained unchanged in the venules. The deviation angle of endothelial cells was nearly parallel to the vessel axis especially in the arterioles.
Conclusions: Change in shape of endothelial cells in the retinal arterioles suggested a high shear stress in the large arterioles, and lower shear stress in the smaller arterioles with a decrease in caliber. In the venules, however, the cell shape was unchanged, and this suggests that the blood flow is fairly steady. (J Jpn Ophthalmol Soc 105:682–686, 2001) Tomoko Tsuruya, Satoru Kimura and Mitsuru Nakazawa Department of Ophthalmology, Hirosaki University PII S0021-5155(01)00515-9
Retinal Hazard from Blue Light Emitting Diode Purpose: To compare the effect of exposure time from a blue (460 nm) light emitting diode (LED) on the morphology of the outer retina and determine conditions where damage occurs. Materials and Methods: Young adult rhesus monkeys were anesthetized, and received blue LED exposure from a modified slit-lamp. A 3 mm beam of 0.85 mW was imaged onto the retina through a lens positioned before the cornea and exposure damage was determined at time intervals of 12 to 90 min. Fundus photography, fluorescein angiography (FAG), retinal tomography (HRT), and s-cone electroretinograms (S-ERG) were recorded at baseline, 2, and 30 days. Results: Two days after 40 min exposure, there was a grey, discolored region, which was overfluorescent in FAG, and an increase in HRT and S-ERG corresponding to the site which was exposed to LED light. In histological examination at 30 days, the LED had caused a marked disruption of the disks of photoreceptor cells, damaged retinal pigment epithelium (RPE) apical villi, and resulted in a loss of RPE melanin after 90 min exposure. Conclusion: A threshold level was found around 40 min. This morphological damage may impair function, and continuous exposure to blue light is potentially dangerous to vision. (J Jpn Ophthalmol Soc 105:687– 695, 2001) Ryouhei Koide*, Takako N Ueda†, William W Dawson‡, GM Hope‡, Ann Ellis§, Don Samuelson§, Toshihiko Ueda*, Shigehiro Iwabuchi*, Shohei Fukuda*, Miou Matsuishi*, Hajime Yasuhara*, Tetsuma Ozawa* and Donald Armstrong*§ *Department of Ophthalmology, School of Medicine, Showa University; †Department of Pharmacology, School of Medicine, Showa University; ‡Department of Ophthalmology, College of Medicine, University of