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Motility and fertilizing capacity of epididymal human spermatozoa in normal and pathological cases
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Citations from the Literature
ception. Duration of infertility < 3 years was associated with higher conception rate (41.1%) than longer duration (28.2%). None of the semen characteristics nor the ATP content predicted the occurrence of pregnancy. Conclusions: Neither conventional sperm characteristics nor semen ATP can predict the occurrence of pregnancy among couples in which the female partner is normal and the male partner has sperm concentration > 20 X 106/ml. Motility and fert&ing capacity of epididymnl human spermatozoa in normal and pathological cases Mathieu C; Guerin J-F; Cognat M; Lejeune H; Pinatel M-C; Lornage J Lab. de Biologie de la Reproduction, Facuite de Medecine 8, Avenue Rockefeller, 69373 Lyon Cedex 08. FRA FERTIL STERIL 1992 5714 (871-876) Objective: To investigate different factors involved in the outcome of in vitro fertilization (IVF) with epididymal spermatozoa in cases of vas deferens agenesis, with particular emphasis on sperm movement parameters. Design: Prospective study in which sperm movement characteristics, level of puncture and sperm preparation technique were studied relative to the outcome of IVF. Setting: Departement de Gynecologie, Oncologie Gynecologique, Senologie, Medecine de la Reproduction, Hopital Edouard-Herriot, Lyon France. Patients: Thirteen couples underwent 15 IVF cycles. In 14 cycles (12 patients) epididymal spermatozoa could be recovered by surgical punctures. Epididymides were taken from 10 subjects in state of cerebral death to study epididymal spermatozoa under physiological status of the epididymis. Results: The cleavage rate in IVF was low (25/158: 15.8%). In seven cases, embryo transfer was possible, leading to two twin pregnancies (4 infants born). Fertilization could be achieved with spermatozoa from the epididymis caput. Motility was paradoxically higher in the proximal epididymis than in the distal epididymis. In healthy epididymides, spermatozoa collected from the caput did not exhibit any progressive motility, whereas progressive motility was always present in the epididymis corpus. Conclusions: Our study showed that forward motility could be observed in the epididymis caput in patients with congenital absence of the vas deferens and that fertilization and ongoing pregnancies could be obtained from caput spermatozoa. On the other hand, in physiological status of the epididymis, forward motility was observed only as from the corpus area, confirming observations in other species. EvaIuatIon of 225 patients undergoingsubzonal insemination for the procurementof fertikation in vitro Fishel S; Timson J; Lisi F; Rinaldi L Department of Obstetrics/Gynaecoiogy, Queen’s Medical Centre, University Hospital, Nottingham NG7 2UH. GBR FERTIL STERIL 1992 57/4 (840-849) Objective: To evaluate subzonal insemination for the procurement of fertilization in infertile males. Design: The spermatozoa of infertile males was used, where possible, for in vitro fertilization (IVF) and subzonal insemination with the same cohort of oocytes obtained from their partner. Setting: An individual unit in a general hospital, Clinica Nomentana, Rome, Int J Gynecol Obstet 40
Italy. Patients: Males who had repeatedly failed to achieve conception in vitro by IVF or in whom seminal parameters were unacceptable for IVF were treated. Interventions: Two hundred twenty-five transvaginal oocyte retrievals were performed. Main Outcome Measures: The incidence of fertilization, cleavage and pregnancy was evaluated in relation to the numbers of spermatozoa injected and the individual spermatozoa parameters. Results: Fertilization occurred in 39% of patients and 16% of eggs and 12 clinical pregnancies were established. Fertilization increased with increasing numbers of spermatozoa injected. Fertilization with subzonal insemination was significantly greater (39%) than after in vitro insemination (6%) (P c 0.0001). Conclusions: Subzonal insemination improved the incidence of fertilization in this series of patients and a new classification of spermatozoa based on total motile count aided prognosis for fertilization. Preparation of oligozoospermicand/or asthenozoospermicsemen for intrauterineimemination using the SpermPrep semen filtration cohmm Ohashi K; Saji F; Wakimoto A; Kato M; Tsutsui T, Tanizawa 0 Obstetrics and Gynecology Department, Osaka University Medical School, l-l-50 Fukushima, Fukushima-ku, Osaka 553. JPN FERTIL STERIL 1992 57/4 (866-870) Objective: To improve the quality of oligozoospermic and/or asthenozoospermic semen by the SpermPrep (Fertility Technologies Inc., Natick, MA) semen filtration column. Design: The SpermPrep column was applied for semen manipulation in oligozoospermia and/or asthenozoospermia (sperm count c20 x 106/ml, sperm motility 40%). After concentration of motile sperm using a 40% Percoll density gradient centrifugation, the sperm suspension was filtered through the SpermPrep column. The percentage yield of motile sperm by the SpermPrep method was compared with those by a two-layer Percoll density gradient (Pharmacia, Uppsala, Sweden) centrifugation and a swim-up method. Infertile couples with poor quality semen were treated with intrauterine insemination (IUI) with motile sperm by the three preparations through three cycles. Setting: Department of Obstetrics and Gynecology, Osaka University Hospital. Patients: Twenty-one couples with long-standing infertility because of poor quality semen. Main Outcome Measure: Recovery of motile sperm, sperm motility and outcome of IUI were evaluated among three semen preparations. Results: Motility was improved by the SpermPrep method in 32 of 33 cases of oligozoospermia and/or asthenozoospermia. Percentage yield of motile sperm by the SpermPrep method was significantly greater than those by the two-layer Percoll density gradient and swim-up methods (42.7 f 4.6 versus 22.1 f 3.1 and 13.8 f 3.5), but there is no significant difference in the sperm motility among three semen preparations. After one treatment cycle for each preparation, 2 of 21 women conceived after IUI with motile sperm separated in the SpermPrep method. Conclusions: The SpermPrep method is an improved semen manipulation method for oligozoospermia and/or asthenozoospermia.
Citations from the Literature
ception. Duration of infertility < 3 years was associated with higher conception rate (41.1%) than longer duration (28.2%). None of the semen characteristics nor the ATP content predicted the occurrence of pregnancy. Conclusions: Neither conventional sperm characteristics nor semen ATP can predict the occurrence of pregnancy among couples in which the female partner is normal and the male partner has sperm concentration > 20 X 106/ml. Motility and fert&ing capacity of epididymnl human spermatozoa in normal and pathological cases Mathieu C; Guerin J-F; Cognat M; Lejeune H; Pinatel M-C; Lornage J Lab. de Biologie de la Reproduction, Facuite de Medecine 8, Avenue Rockefeller, 69373 Lyon Cedex 08. FRA FERTIL STERIL 1992 5714 (871-876) Objective: To investigate different factors involved in the outcome of in vitro fertilization (IVF) with epididymal spermatozoa in cases of vas deferens agenesis, with particular emphasis on sperm movement parameters. Design: Prospective study in which sperm movement characteristics, level of puncture and sperm preparation technique were studied relative to the outcome of IVF. Setting: Departement de Gynecologie, Oncologie Gynecologique, Senologie, Medecine de la Reproduction, Hopital Edouard-Herriot, Lyon France. Patients: Thirteen couples underwent 15 IVF cycles. In 14 cycles (12 patients) epididymal spermatozoa could be recovered by surgical punctures. Epididymides were taken from 10 subjects in state of cerebral death to study epididymal spermatozoa under physiological status of the epididymis. Results: The cleavage rate in IVF was low (25/158: 15.8%). In seven cases, embryo transfer was possible, leading to two twin pregnancies (4 infants born). Fertilization could be achieved with spermatozoa from the epididymis caput. Motility was paradoxically higher in the proximal epididymis than in the distal epididymis. In healthy epididymides, spermatozoa collected from the caput did not exhibit any progressive motility, whereas progressive motility was always present in the epididymis corpus. Conclusions: Our study showed that forward motility could be observed in the epididymis caput in patients with congenital absence of the vas deferens and that fertilization and ongoing pregnancies could be obtained from caput spermatozoa. On the other hand, in physiological status of the epididymis, forward motility was observed only as from the corpus area, confirming observations in other species. EvaIuatIon of 225 patients undergoingsubzonal insemination for the procurementof fertikation in vitro Fishel S; Timson J; Lisi F; Rinaldi L Department of Obstetrics/Gynaecoiogy, Queen’s Medical Centre, University Hospital, Nottingham NG7 2UH. GBR FERTIL STERIL 1992 57/4 (840-849) Objective: To evaluate subzonal insemination for the procurement of fertilization in infertile males. Design: The spermatozoa of infertile males was used, where possible, for in vitro fertilization (IVF) and subzonal insemination with the same cohort of oocytes obtained from their partner. Setting: An individual unit in a general hospital, Clinica Nomentana, Rome, Int J Gynecol Obstet 40
Italy. Patients: Males who had repeatedly failed to achieve conception in vitro by IVF or in whom seminal parameters were unacceptable for IVF were treated. Interventions: Two hundred twenty-five transvaginal oocyte retrievals were performed. Main Outcome Measures: The incidence of fertilization, cleavage and pregnancy was evaluated in relation to the numbers of spermatozoa injected and the individual spermatozoa parameters. Results: Fertilization occurred in 39% of patients and 16% of eggs and 12 clinical pregnancies were established. Fertilization increased with increasing numbers of spermatozoa injected. Fertilization with subzonal insemination was significantly greater (39%) than after in vitro insemination (6%) (P c 0.0001). Conclusions: Subzonal insemination improved the incidence of fertilization in this series of patients and a new classification of spermatozoa based on total motile count aided prognosis for fertilization. Preparation of oligozoospermicand/or asthenozoospermicsemen for intrauterineimemination using the SpermPrep semen filtration cohmm Ohashi K; Saji F; Wakimoto A; Kato M; Tsutsui T, Tanizawa 0 Obstetrics and Gynecology Department, Osaka University Medical School, l-l-50 Fukushima, Fukushima-ku, Osaka 553. JPN FERTIL STERIL 1992 57/4 (866-870) Objective: To improve the quality of oligozoospermic and/or asthenozoospermic semen by the SpermPrep (Fertility Technologies Inc., Natick, MA) semen filtration column. Design: The SpermPrep column was applied for semen manipulation in oligozoospermia and/or asthenozoospermia (sperm count c20 x 106/ml, sperm motility 40%). After concentration of motile sperm using a 40% Percoll density gradient centrifugation, the sperm suspension was filtered through the SpermPrep column. The percentage yield of motile sperm by the SpermPrep method was compared with those by a two-layer Percoll density gradient (Pharmacia, Uppsala, Sweden) centrifugation and a swim-up method. Infertile couples with poor quality semen were treated with intrauterine insemination (IUI) with motile sperm by the three preparations through three cycles. Setting: Department of Obstetrics and Gynecology, Osaka University Hospital. Patients: Twenty-one couples with long-standing infertility because of poor quality semen. Main Outcome Measure: Recovery of motile sperm, sperm motility and outcome of IUI were evaluated among three semen preparations. Results: Motility was improved by the SpermPrep method in 32 of 33 cases of oligozoospermia and/or asthenozoospermia. Percentage yield of motile sperm by the SpermPrep method was significantly greater than those by the two-layer Percoll density gradient and swim-up methods (42.7 f 4.6 versus 22.1 f 3.1 and 13.8 f 3.5), but there is no significant difference in the sperm motility among three semen preparations. After one treatment cycle for each preparation, 2 of 21 women conceived after IUI with motile sperm separated in the SpermPrep method. Conclusions: The SpermPrep method is an improved semen manipulation method for oligozoospermia and/or asthenozoospermia.