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Motor responses of the isolated amphibian endodermal cells to the chemical environment
Cell surface and cell interactions (340-362)
340
341
D I P F E R E N T I A T I O N (DP) OP L Y ~ P H O C Y T E S A N D R E C E P T O R S TO A C E T Y L C H O L I N E ( A C I
MOTOR RESPONSES OF THE ISOLATED AMPHIBIAN ENDODERMAL CELLS TO THE CHEMICAL ENVIRONMENT. Merja Bl~uer and J. Kohonen. Department of Biology. University of Turku, SF-20500 Turku, Finland.
GeAfoninaIV.BordonosIL.Ku~un.Kiev Medical Institute,Kiev
252057,USSR.
M u l t i p c t e n t i a l i t y of the l y m p h o i d c e l l s is d e t e r m i n e d by the s t r u c ture of s u r f a c e r e c e p t o r ' s f o r m a t i o n . T h e c h a n g e of t h e m o c c u r s u n d e r the p r o c e s s of s p e c i a l i z a t i o n a n d D R The s p e c i a l r o l e i n D P of i m m u n c c o m p a t e n t CLs p l a y s n e u r o - e n d o c r i n e - i m m u n e i n t e r a c t i o n s . S u b p o p u l a t i o n of l y m p h o c y t e s d i f f e r s b y c o n c e p t on m e m b r a n e r e c e p t o r s to A C , t h e r o l e of w h i c h in n e u r o - i ~ m u n e c o o p e r a t i o n is d i s c u s s e d . T h e a c t i o n of t o x i c b a c t e r i a l s u b s t a n c e s w e r e s t u d i e d on the s u b j e c t of t h e i r l y m p h o i d r e c e p t o r s a c t i v i t y to AC i n d y n a m i c of ImResp. The i n c r e a s i n g of t h e i r a c t i v i t y in the i n d u c t i o n p h a s e of i m m u n o g e n e s e u n d e r c l o n e s f o r m a t i o n is s h o w n ~ H e n f o l l o w s the d e c r e a s i n g h e l p e r ' s act i v i t y in p r o d u c t i o n p e r i o d on the f i n a l s t a g e s of D P w i t h p r e f e r a b l e g e n e r a t i o n of m e m o r y cells and s u p pressors.We suppose,that under clone A G r e a c t i v e CLs D P f r o m the c e l l s prec u s s o r s the r e c e p t o r ' s s e n s i t i v i t y to AC on l y m p h o i d m e m b r a n e is r e d u c e d . 121S
Isolated endodermal cells of Triturus vulgaris gastrulae together with Holtfreter solution were placed in either bacteriological polystyrene (PS) or tissue culture (NunclonR) dishes. The cells spread and attached to the bottoms of the dishes in a few hours. The responses of the cells to the temporary changes in their chemical environment were studied by pipetting a small amount of the test solution on a cell. In the PS-dishes trypsin, protease, albumin, N-acetylglucosamine, glucosamine, tryptamine, 5-hydroxytryptamine and heparin caused within seconds cell detachment and contraction of pseudopodes. The reaction could be repeated several times at intervals of some minutes. Phosphatase, glucose, saccharose and tryptophan had no effect. The cells on the Nunclon-surface did not respond to any of the substances tested. The results support the concept that extracellular matrix components regulate attachment and movement of the embryonic cells in vivo.
340
341
D I P F E R E N T I A T I O N (DP) OP L Y ~ P H O C Y T E S A N D R E C E P T O R S TO A C E T Y L C H O L I N E ( A C I
MOTOR RESPONSES OF THE ISOLATED AMPHIBIAN ENDODERMAL CELLS TO THE CHEMICAL ENVIRONMENT. Merja Bl~uer and J. Kohonen. Department of Biology. University of Turku, SF-20500 Turku, Finland.
GeAfoninaIV.BordonosIL.Ku~un.Kiev Medical Institute,Kiev
252057,USSR.
M u l t i p c t e n t i a l i t y of the l y m p h o i d c e l l s is d e t e r m i n e d by the s t r u c ture of s u r f a c e r e c e p t o r ' s f o r m a t i o n . T h e c h a n g e of t h e m o c c u r s u n d e r the p r o c e s s of s p e c i a l i z a t i o n a n d D R The s p e c i a l r o l e i n D P of i m m u n c c o m p a t e n t CLs p l a y s n e u r o - e n d o c r i n e - i m m u n e i n t e r a c t i o n s . S u b p o p u l a t i o n of l y m p h o c y t e s d i f f e r s b y c o n c e p t on m e m b r a n e r e c e p t o r s to A C , t h e r o l e of w h i c h in n e u r o - i ~ m u n e c o o p e r a t i o n is d i s c u s s e d . T h e a c t i o n of t o x i c b a c t e r i a l s u b s t a n c e s w e r e s t u d i e d on the s u b j e c t of t h e i r l y m p h o i d r e c e p t o r s a c t i v i t y to AC i n d y n a m i c of ImResp. The i n c r e a s i n g of t h e i r a c t i v i t y in the i n d u c t i o n p h a s e of i m m u n o g e n e s e u n d e r c l o n e s f o r m a t i o n is s h o w n ~ H e n f o l l o w s the d e c r e a s i n g h e l p e r ' s act i v i t y in p r o d u c t i o n p e r i o d on the f i n a l s t a g e s of D P w i t h p r e f e r a b l e g e n e r a t i o n of m e m o r y cells and s u p pressors.We suppose,that under clone A G r e a c t i v e CLs D P f r o m the c e l l s prec u s s o r s the r e c e p t o r ' s s e n s i t i v i t y to AC on l y m p h o i d m e m b r a n e is r e d u c e d . 121S
Isolated endodermal cells of Triturus vulgaris gastrulae together with Holtfreter solution were placed in either bacteriological polystyrene (PS) or tissue culture (NunclonR) dishes. The cells spread and attached to the bottoms of the dishes in a few hours. The responses of the cells to the temporary changes in their chemical environment were studied by pipetting a small amount of the test solution on a cell. In the PS-dishes trypsin, protease, albumin, N-acetylglucosamine, glucosamine, tryptamine, 5-hydroxytryptamine and heparin caused within seconds cell detachment and contraction of pseudopodes. The reaction could be repeated several times at intervals of some minutes. Phosphatase, glucose, saccharose and tryptophan had no effect. The cells on the Nunclon-surface did not respond to any of the substances tested. The results support the concept that extracellular matrix components regulate attachment and movement of the embryonic cells in vivo.