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Mouse B16-F10 melanoma cells proliferation is dependent on fatty acid synthase
OOOO Volume 114, Number 4 retention of deciduous teeth, oligodontia, periodontal disease and caries. Restorative, periodontal and surgical treatments were performed. After this, the patient was referred for orthodontic treatment. Early diagnosis as well as treatment and control of the patient’s hormone levels are important to avoid complications in several organ and systems. Late diagnosed CH is associated with changes in dental development.
REACTIVE POST-RADIOTHERAPY BONE FORMATION IN THE MAXILLA: A CASE REPORT OF AN UNUSUAL PRESENTATION Mariano FV1, Gondak RO1, Santos-Silva AR1, Corrêa MB2, Almeida OP1, Lopes MA1 - 1FOP/UNICAMP - DIAGNÓSTICO ORAL, 2HOSPITAL DOS FORNECEDORES DE CANA - CABEÇA E PESCOÇO Objective: High-dose radiation therapy affects bone metabolism and therefore post-radiotherapy bone formation is an uncommon finding. This case describes an unusual ossification in the maxillary region identified after head and neck radiotherapy. Study design, results: A 45 year-old female patient was submitted to maxillary surgical resection and orbital exenteration due to squamous cell carcinoma. Radiotherapy post surgery was performed because of compromised surgical margins. After four months of the conclusion of radiotherapy, the patient presented a bone exposition at surgical site. Surprisingly, the oral examination and computed tomography revealed a new formation of the right palatine vault and bone formation filling nasal and orbital cavity. The incisional biopsy discarded residual disease or osteoradionecrosis and showed normal tissue. Conclusion: To the best of the authors’ knowledge, this is the first case of wide bone formation as an early effect of head and neck radiotherapy. The current patient continues being accompanied monthly.
MOUSE B16-F10 MELANOMA CELLS PROLIFERATION IS DEPENDENT ON FATTY ACID SYNTHASE Ortega R1, Agostini M2, Bastos DC3, Graner E, Zecchin KG - 1PIRACICABA DENTAL SCHOOL, UNIVERSITY OF CAMPINAS (UNICAMP) - DIAGNÓSTICO ORAL, 2SCHOLL OF DENTISTRY, FEDERAL UNIVERSITY OF RIO DE JANEIRO (UFRJ) - DEPARTMENT OF PATHOLOGY AND ORAL DIAGNOSIS, 3PIRACICABA DENTAL SCHOOL, UNIVERSITY OF CAMPINAS (UNICAMP) - ORAL DIAGNOSIS Objectives: The aim of this work was to investigate the effects fatty acid synthase (FASN) inhibitors over mouse B16F10 melanoma cells proliferation. Study design: After treatment with cerulenin or C75, we analyzed cell viability, proliferation rate, apoptosis and the levels of proteins related to G0/G1 phase of cell cycle. Results: Both FASN inhibitors significantly reduced in vitro melanoma cells proliferation in a dose dependent manner, as well as increased the apoptotic rate. Cell cycle arrest was evidenced by a significant increase in G0/G1 phase in comparison with untreated cells. Western blotting analysis of cerulenin or C75treated cells showed a significant accumulation of the tumor suppressor proteins p21WAF1/Cip1 and p27Kip1, together with decreased amounts of Skp2, essential for the proteasomal degradation of p27Kip1. Conclusions: Taken together, these findings demonstrate that B16-F10 melanoma cells proliferation is dependent on FASN. Supported by FAPESP (10/52635-1).
ORAL ABSTRACTS Abstracts e101 SIALOLITH IN PAROTID GLAND AND SUBLINGUAL GLAND: CASE REPORT Carvalho H1, Akra I1, Gomes W1, Bahia TPS2, Maciel RM2, Ramos MEB2, Israel MS2 - 1UERJ - FACULDADE DE ODONTOLOGIA, 2UERJ - DEPARTAMENTO DE DIAGNÓSTICO ORAL E CIRURGIA Sialoliths are most common in submandibular gland. Sialoliths in parotid and sublingual glands are uncommon. The aim of this study to report two cases of sialoliths, affecting the parotid and another affecting sublingual gland. Case 1- A 80 year-old Caucasian man had a week painful swelling on mouth floor, blocking the use of the prosthesis. The physical examination showed the presence of painful palpable lymph nodes and a swelling with red surface on mouth floor. The panoramic and occlusal radiographs showed the sialolith, which was surgically removed. Case 2- A 56 year-old black woman had a swelling with no symptoms in parotid gland region that kept growing for 1 year. The oral examination showed a swelling on the parotid papilla, with hard consistency. The panoramic radiograph showed the sialolith, which was surgically removed. After one year no sign of recurrence were noted in both cases.
IMMUNOEXPRESSION OF 〈2-INTEGRIN AND HSP47 IN HEREDITARY GINGIVAL FIBROMATOSIS AND GINGIVAL FIBROMATOSIS-ASSOCIATED DENTAL ABNORMALITIES Borges-Pêgo SP1, Vieira Júnior JR, Santos CO, Costa CD1, Paranaíba LMR1, Coletta RD2, MartelliJunior H1 - 1UNIVERSIDADE ESTADUAL DE MONTES CLAROS - UNIMONTES - ODONTOLOGIA, 2UNIVERSIDADE ESTADUAL DE CAMPINAS - UNICAMP The purpose of the study was to investigate the expression of the ␣2-integrin subunit and heat shock protein 47 (Hsp47). Immunohistochemistry was performed in specimens from two unrelated families with hereditary gingival fibromatosis and from one family with a gingival fibromatosis-associated dental abnormality. Normal gingival (NG) were used for comparison. Immunoreactivity for ␣2-integrin and Hsp47 was observed in the nucleus of epithelial cells of both the basal and suprabasal layer and a more discreet signal was noted in connective tissue in all samples. Our results confirmed the heterogeneity of gingival fibromatosis (GF), such that similar patterns of expression of the condition may show differences in the expression of proteins such as Hsp47. Although no difference in ␣2-integrin expression was observed between GF and NG groups, future studies are necessary to determine the exact role of this protein in the various forms of GF and whether it contributes to GF pathogenesis.
METASTASIS OF HEPATOCELULLAR CARCINOMA TO THE ORAL CAVITY: CASE REPORT AND REVIEW OF THE LITERATURE Kang SR1, Choi EJ1, Kim HS2,3, Kim J2,3, Kim HJ1,3 - 1COLLEGE OF DENTISTRY, YONSEI UNIVERSITY, SEOUL, SOUTH KOREA - DEPARTMENT OF ORAL AND MAXILLOFACIAL SURGERY, 2COLLEGE OF DENTISTRY, YONSEI UNIVERSITY, SEOUL, SOUTH KOREA DEPARTMENT OF ORAL PATHOLOGY, 3COLLEGE OF DENTISTRY, YONSEI UNIVERSITY, SEOUL, SOUTH KOREA - ORAL CANCER RESEARCH INSTITUTE Metastatic malignant neoplasms to the oral cavity accounts for 1% or more of all malignant oral lesions. These metastases usually arise from a breast, lung, kidney, colon, adrenal gland or prostate primary carcinoma, leaving the liver as a rare primary
REACTIVE POST-RADIOTHERAPY BONE FORMATION IN THE MAXILLA: A CASE REPORT OF AN UNUSUAL PRESENTATION Mariano FV1, Gondak RO1, Santos-Silva AR1, Corrêa MB2, Almeida OP1, Lopes MA1 - 1FOP/UNICAMP - DIAGNÓSTICO ORAL, 2HOSPITAL DOS FORNECEDORES DE CANA - CABEÇA E PESCOÇO Objective: High-dose radiation therapy affects bone metabolism and therefore post-radiotherapy bone formation is an uncommon finding. This case describes an unusual ossification in the maxillary region identified after head and neck radiotherapy. Study design, results: A 45 year-old female patient was submitted to maxillary surgical resection and orbital exenteration due to squamous cell carcinoma. Radiotherapy post surgery was performed because of compromised surgical margins. After four months of the conclusion of radiotherapy, the patient presented a bone exposition at surgical site. Surprisingly, the oral examination and computed tomography revealed a new formation of the right palatine vault and bone formation filling nasal and orbital cavity. The incisional biopsy discarded residual disease or osteoradionecrosis and showed normal tissue. Conclusion: To the best of the authors’ knowledge, this is the first case of wide bone formation as an early effect of head and neck radiotherapy. The current patient continues being accompanied monthly.
MOUSE B16-F10 MELANOMA CELLS PROLIFERATION IS DEPENDENT ON FATTY ACID SYNTHASE Ortega R1, Agostini M2, Bastos DC3, Graner E, Zecchin KG - 1PIRACICABA DENTAL SCHOOL, UNIVERSITY OF CAMPINAS (UNICAMP) - DIAGNÓSTICO ORAL, 2SCHOLL OF DENTISTRY, FEDERAL UNIVERSITY OF RIO DE JANEIRO (UFRJ) - DEPARTMENT OF PATHOLOGY AND ORAL DIAGNOSIS, 3PIRACICABA DENTAL SCHOOL, UNIVERSITY OF CAMPINAS (UNICAMP) - ORAL DIAGNOSIS Objectives: The aim of this work was to investigate the effects fatty acid synthase (FASN) inhibitors over mouse B16F10 melanoma cells proliferation. Study design: After treatment with cerulenin or C75, we analyzed cell viability, proliferation rate, apoptosis and the levels of proteins related to G0/G1 phase of cell cycle. Results: Both FASN inhibitors significantly reduced in vitro melanoma cells proliferation in a dose dependent manner, as well as increased the apoptotic rate. Cell cycle arrest was evidenced by a significant increase in G0/G1 phase in comparison with untreated cells. Western blotting analysis of cerulenin or C75treated cells showed a significant accumulation of the tumor suppressor proteins p21WAF1/Cip1 and p27Kip1, together with decreased amounts of Skp2, essential for the proteasomal degradation of p27Kip1. Conclusions: Taken together, these findings demonstrate that B16-F10 melanoma cells proliferation is dependent on FASN. Supported by FAPESP (10/52635-1).
ORAL ABSTRACTS Abstracts e101 SIALOLITH IN PAROTID GLAND AND SUBLINGUAL GLAND: CASE REPORT Carvalho H1, Akra I1, Gomes W1, Bahia TPS2, Maciel RM2, Ramos MEB2, Israel MS2 - 1UERJ - FACULDADE DE ODONTOLOGIA, 2UERJ - DEPARTAMENTO DE DIAGNÓSTICO ORAL E CIRURGIA Sialoliths are most common in submandibular gland. Sialoliths in parotid and sublingual glands are uncommon. The aim of this study to report two cases of sialoliths, affecting the parotid and another affecting sublingual gland. Case 1- A 80 year-old Caucasian man had a week painful swelling on mouth floor, blocking the use of the prosthesis. The physical examination showed the presence of painful palpable lymph nodes and a swelling with red surface on mouth floor. The panoramic and occlusal radiographs showed the sialolith, which was surgically removed. Case 2- A 56 year-old black woman had a swelling with no symptoms in parotid gland region that kept growing for 1 year. The oral examination showed a swelling on the parotid papilla, with hard consistency. The panoramic radiograph showed the sialolith, which was surgically removed. After one year no sign of recurrence were noted in both cases.
IMMUNOEXPRESSION OF 〈2-INTEGRIN AND HSP47 IN HEREDITARY GINGIVAL FIBROMATOSIS AND GINGIVAL FIBROMATOSIS-ASSOCIATED DENTAL ABNORMALITIES Borges-Pêgo SP1, Vieira Júnior JR, Santos CO, Costa CD1, Paranaíba LMR1, Coletta RD2, MartelliJunior H1 - 1UNIVERSIDADE ESTADUAL DE MONTES CLAROS - UNIMONTES - ODONTOLOGIA, 2UNIVERSIDADE ESTADUAL DE CAMPINAS - UNICAMP The purpose of the study was to investigate the expression of the ␣2-integrin subunit and heat shock protein 47 (Hsp47). Immunohistochemistry was performed in specimens from two unrelated families with hereditary gingival fibromatosis and from one family with a gingival fibromatosis-associated dental abnormality. Normal gingival (NG) were used for comparison. Immunoreactivity for ␣2-integrin and Hsp47 was observed in the nucleus of epithelial cells of both the basal and suprabasal layer and a more discreet signal was noted in connective tissue in all samples. Our results confirmed the heterogeneity of gingival fibromatosis (GF), such that similar patterns of expression of the condition may show differences in the expression of proteins such as Hsp47. Although no difference in ␣2-integrin expression was observed between GF and NG groups, future studies are necessary to determine the exact role of this protein in the various forms of GF and whether it contributes to GF pathogenesis.
METASTASIS OF HEPATOCELULLAR CARCINOMA TO THE ORAL CAVITY: CASE REPORT AND REVIEW OF THE LITERATURE Kang SR1, Choi EJ1, Kim HS2,3, Kim J2,3, Kim HJ1,3 - 1COLLEGE OF DENTISTRY, YONSEI UNIVERSITY, SEOUL, SOUTH KOREA - DEPARTMENT OF ORAL AND MAXILLOFACIAL SURGERY, 2COLLEGE OF DENTISTRY, YONSEI UNIVERSITY, SEOUL, SOUTH KOREA DEPARTMENT OF ORAL PATHOLOGY, 3COLLEGE OF DENTISTRY, YONSEI UNIVERSITY, SEOUL, SOUTH KOREA - ORAL CANCER RESEARCH INSTITUTE Metastatic malignant neoplasms to the oral cavity accounts for 1% or more of all malignant oral lesions. These metastases usually arise from a breast, lung, kidney, colon, adrenal gland or prostate primary carcinoma, leaving the liver as a rare primary