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Movement characteristics of human epididymal sperm used for fertilization of human oocytes in vitro
Citations from the Literature
of human epididymal sperm used for fertilization of Inmu0 oocytes in vitm
Movement ebara&rI&es
Davis RO, Overstreet JW; Asch RH; Ord T; Silber SJ Division of Reproductive
Biology and Medicine,
University
339
with fresh semen pooled with cryopreserved occasionally improved semen samples for the treatment of oligoasthenospermia results in an improved PR.
of
California. Davis, CA 95616, USA
FERTIL STERIL 1991 56/6 (1128-I 135) Study Objective: To develop mathematical models using kinematic parameters from Computer-Aided Sperm Analysis (CASA) that predict the fertilization rate of sperm recovered from the caput epididymidis and to test the hypothesis that fertilization was enhanced by the presence of specific sperm subpopulations in the inseminate. Setting: In vitro fertilization (IVF) program. Patients: Thirteen patients with congenital absence of the vas deferens provided epididymal sperm for IVF as well as for CASA. Results: The mathematical model that was most predictive of fertilization rates included kinematic parameters of the epididymal aspirate (percent motility), the inseminate used for IVF (curvilinear velocity (VCL)) and the change in sperm movement after in vitro processing by the mini-Percoll technique (difference in amplitude of lateral head displacement (ALH)). Multivariate cluster analysis revealed that inseminates that resulted in higher fertilization rates had subpopulations of sperm that were characterized by high VCL and high mean angular displacement, as well as a greater change in ALH after processing. Conclusions: In vitro fertilization with epididymal sperm was more likely to succeed when the sperm population that was initially aspirated had a higher proportion of motile cells and when these sperm were capable of capacitation in vitro as indicated by the appearance of sperm subpopulations with motility that resembled hyperactivation.
Cryopreservntionofthe 0eeasiomBy improved semen samples for intrPaterine hseminetion: A ww approach in the treatment of idio@ic m8le infertility
Aboulghar MA; Mansour RT; Serour GI; Sattar MA, Elattar I Egyptian IVF-ET Center, 85 Maadi Zeraie Road, Maadi, Cairo, EGY
FERTIL STERIL 1991 56/6 (1151-1155) Objective: To assess the value of treating idiopathic male infertility by intrauterine insemination (IUI) of the occasionally improved cryopreserved semen. Design: Two groups of idiopathic oligospetmic patients were chosen at random and treated by IUI using processed fresh semen in group A and the best available cryopreserved semen samples pooled with fresh samples in group B. Setting: Egyptian IVF-ET Centre, Maadi, Cairo, Egypt. Patients, Participants: One hundred tifty infertile couples because of idiopathic oligoasthenospermia. Intervention: Intrauterine insemination. Main Outcome Measure: The pregnancy rate was evaluated after an average of three treatment cycles. Results: The pregnancy rate (PR) was significantly higher in group B when compared with group A. The improvement in the PR was highly significant in the subgroup of patients for whom reasonable semen samples could be collected and cryopreserved. Conclusions: Our study indicates that IUI
VIROLOGY Human immunodeficiency virus infection in women
Spence M.R.; Reboli A.C. Department of Obstetrics/Gynecology, Broad and Vine Streets, Philadelphia,
Hahnemann Hospital, PA 19102-1192, USA
ANN INTERN MED 1991 115110(827-829) The AIDS epidemic has now entered the second decade. Numerous changes have occurred during first 10 years, including definition of the causative agent and development of many therapies for both the primary disease and its complications. The populations at risk have also changed, and women now represent a major group, the gender-specific issues of whom need to be addressed. This demographic change may require the consideration of changes in diagnostic criteria and the involvement of women in clinical trials. Large multidisciplinary, longitudinal studies of women infected with HIV must be done.
High risk of HIV-1 infection for fti-born twins Goedert JJ; Duliege A-M; Amos CI; Felton S; Biggar RJ Viral Epidemiology RockviNe. MD. USA
Section,
National
Cancer
Institute,
LANCET 1991 338/8781 (1471-1475) To examine the epidemiology and natural history of motherto-infant transmission of human immunodeficiency virus type 1 (HIV-l), especially genetic and intrapartum exposure factors, we obtained data on twins and triplets born to women infected with the virus. Forty investigators in nine countries contributed demographic, clinical and epidemiological data on 100 sets of twins and 1 set of triplets. Among the 66 evaluable sets, HIV-l infection was more common in first-born than in second-born twins (P = 0.004). In 22 sets, only one twin was infected (18 first-born, 4 second-born). 56% of first-born twins delivered vaginally and 38% of first-born twins delivered by cesarean were infected, compared with 19% of second-born twins delivered by either route. HIV-l infection status tended to be concordant in more monozygotic (14 of 17 sets) than dizygotic (26 of 43) sets, but the frequency and clinical signs of HIV-l-related disease were similar in only 3 of the 10 sets with both children infected. These findings suggest that some infants may be infected in utero before labor but that a substantial proportion of HIV-l transmission occurs as the first twin encounters the cervix and birth canal. Such measures as cleansing of the birth canal and cesarean delivery before membrane rupture might reduce the risk of transmission for infants born to HIV-l infected women and should be the subjects of controlled clinical trials. Cesarean section should not be regarded as a wholly preventive measure, however, since substantial proportions of both first-born and second-born twins delivered in this way were infected. Int J Gynecol Obstet 38
of human epididymal sperm used for fertilization of Inmu0 oocytes in vitm
Movement ebara&rI&es
Davis RO, Overstreet JW; Asch RH; Ord T; Silber SJ Division of Reproductive
Biology and Medicine,
University
339
with fresh semen pooled with cryopreserved occasionally improved semen samples for the treatment of oligoasthenospermia results in an improved PR.
of
California. Davis, CA 95616, USA
FERTIL STERIL 1991 56/6 (1128-I 135) Study Objective: To develop mathematical models using kinematic parameters from Computer-Aided Sperm Analysis (CASA) that predict the fertilization rate of sperm recovered from the caput epididymidis and to test the hypothesis that fertilization was enhanced by the presence of specific sperm subpopulations in the inseminate. Setting: In vitro fertilization (IVF) program. Patients: Thirteen patients with congenital absence of the vas deferens provided epididymal sperm for IVF as well as for CASA. Results: The mathematical model that was most predictive of fertilization rates included kinematic parameters of the epididymal aspirate (percent motility), the inseminate used for IVF (curvilinear velocity (VCL)) and the change in sperm movement after in vitro processing by the mini-Percoll technique (difference in amplitude of lateral head displacement (ALH)). Multivariate cluster analysis revealed that inseminates that resulted in higher fertilization rates had subpopulations of sperm that were characterized by high VCL and high mean angular displacement, as well as a greater change in ALH after processing. Conclusions: In vitro fertilization with epididymal sperm was more likely to succeed when the sperm population that was initially aspirated had a higher proportion of motile cells and when these sperm were capable of capacitation in vitro as indicated by the appearance of sperm subpopulations with motility that resembled hyperactivation.
Cryopreservntionofthe 0eeasiomBy improved semen samples for intrPaterine hseminetion: A ww approach in the treatment of idio@ic m8le infertility
Aboulghar MA; Mansour RT; Serour GI; Sattar MA, Elattar I Egyptian IVF-ET Center, 85 Maadi Zeraie Road, Maadi, Cairo, EGY
FERTIL STERIL 1991 56/6 (1151-1155) Objective: To assess the value of treating idiopathic male infertility by intrauterine insemination (IUI) of the occasionally improved cryopreserved semen. Design: Two groups of idiopathic oligospetmic patients were chosen at random and treated by IUI using processed fresh semen in group A and the best available cryopreserved semen samples pooled with fresh samples in group B. Setting: Egyptian IVF-ET Centre, Maadi, Cairo, Egypt. Patients, Participants: One hundred tifty infertile couples because of idiopathic oligoasthenospermia. Intervention: Intrauterine insemination. Main Outcome Measure: The pregnancy rate was evaluated after an average of three treatment cycles. Results: The pregnancy rate (PR) was significantly higher in group B when compared with group A. The improvement in the PR was highly significant in the subgroup of patients for whom reasonable semen samples could be collected and cryopreserved. Conclusions: Our study indicates that IUI
VIROLOGY Human immunodeficiency virus infection in women
Spence M.R.; Reboli A.C. Department of Obstetrics/Gynecology, Broad and Vine Streets, Philadelphia,
Hahnemann Hospital, PA 19102-1192, USA
ANN INTERN MED 1991 115110(827-829) The AIDS epidemic has now entered the second decade. Numerous changes have occurred during first 10 years, including definition of the causative agent and development of many therapies for both the primary disease and its complications. The populations at risk have also changed, and women now represent a major group, the gender-specific issues of whom need to be addressed. This demographic change may require the consideration of changes in diagnostic criteria and the involvement of women in clinical trials. Large multidisciplinary, longitudinal studies of women infected with HIV must be done.
High risk of HIV-1 infection for fti-born twins Goedert JJ; Duliege A-M; Amos CI; Felton S; Biggar RJ Viral Epidemiology RockviNe. MD. USA
Section,
National
Cancer
Institute,
LANCET 1991 338/8781 (1471-1475) To examine the epidemiology and natural history of motherto-infant transmission of human immunodeficiency virus type 1 (HIV-l), especially genetic and intrapartum exposure factors, we obtained data on twins and triplets born to women infected with the virus. Forty investigators in nine countries contributed demographic, clinical and epidemiological data on 100 sets of twins and 1 set of triplets. Among the 66 evaluable sets, HIV-l infection was more common in first-born than in second-born twins (P = 0.004). In 22 sets, only one twin was infected (18 first-born, 4 second-born). 56% of first-born twins delivered vaginally and 38% of first-born twins delivered by cesarean were infected, compared with 19% of second-born twins delivered by either route. HIV-l infection status tended to be concordant in more monozygotic (14 of 17 sets) than dizygotic (26 of 43) sets, but the frequency and clinical signs of HIV-l-related disease were similar in only 3 of the 10 sets with both children infected. These findings suggest that some infants may be infected in utero before labor but that a substantial proportion of HIV-l transmission occurs as the first twin encounters the cervix and birth canal. Such measures as cleansing of the birth canal and cesarean delivery before membrane rupture might reduce the risk of transmission for infants born to HIV-l infected women and should be the subjects of controlled clinical trials. Cesarean section should not be regarded as a wholly preventive measure, however, since substantial proportions of both first-born and second-born twins delivered in this way were infected. Int J Gynecol Obstet 38