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MS101 EVIDENCE OF INCREASED SDLDL PARTICLES DURING ACUTE INFECTION WITH EPSTEIN–BARR VIRUS
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Atherosclerosis Supplements 11, no. 2 (2010) 109–222
Conclusion: The study demonstrates the efficacy of dietary n-3 PUFA to change the pro-atherogenic LDL phenotype to less atherogenic in patients with MetS. Supported by the FW6 EU LIPGENE. MS98 CHOLESTEROL-LOWERING EFFECT OF FORMALDEHYDE INJECTIONS IN SUPER-LOW DOSES E. Tereshina1,2 , S. Ivanenko2 . 1 Russian Clinical and Scientific Center of Gerontology, Moscow, Russia, 2 World Wide Medical Assistance, Zug, Switzerland Formaldehyde (FH) effects on tumor regression and tuberculosis recovery and protection have been registered in animals. Nonspecific homeostatic ways of FM action in the living organism were supposed. So, it was interesting to evaluate FM effect on the cholesterol homeostasis in humans. Toxicity of FM was assessed on 56 outbred ICR mice. In human studies a group of 12 volunteers with various indices of total cholesterol (TC) content (4.3−6.8 mmol/L) was formed. FM was injected i.m. in super low doses for five times with intervals at 7, 21, 30 and 60 days. Samples of blood were taken at 7, 28, 35 and 65 days from the beginning of the experiment. In 8 cases stable decrease in the TC content was achieved at 35 day, in 4 cases − at 7 day. In the whole group final indices of TC were 4.6−2.8 mmol/L. Within cells FM is a substrate for catalase that is located in a peroxisome and involved in the oxidation of fatty acids and synthesis of cholesterol and bile acids. At the very low values of substrate catalase reaction rate increases very rapidly. Modulating effect of FM may appear in gluthation formation and action as they both depend on CH3 group translocations. Thus, FM in super low doses may influence cholesterol homeostasis through modulation of intracellular red/ox potential. MS99 SEASONAL DIFFERENCES IN TRANS-SIALIDASES ACTIVITY E. Orekhova1 , V. Myasoedova1 , I. Sobenin2 , A. Orekhov1 . 1 Inst for Atherosclerosis Res, 2 Inst for General Pathology and Pathophysiology, Moscow, Russia We have earlier found that human blood contains a soluble enzyme, belonging by its donor-acceptor properties to trans-sialidases. Incubation of low density lipoprotein (LDL) with trans-sialidase results in a loss of sialic acid from lipoprotein particles. Desialylated low density lipoprotein induces cholesterol esters accumulation, the main process accompanying atherosclerosis development. Lipoprotein-deficient serum obtained by ultracentrifugation was used for isolation desialylating enzyme by the affinity chromatography. Serum sample was applied on column with a-2,8-Neu5Ac-sepharose. After washing, sorbentbound protein fraction was eluted with 50 mM sialic acid. Electrophoresis of eluted fraction under denaturating conditions revealed the presence of only one protein band with molecular weight about 67 kDa. Activity of this enzyme was measured by transfer of [H3 ] labeled sialic acid from immobilized donor to soluble acceptor. We revealed seasonal differences in trans-sialidases activity. It is well known that coronary heart disease risk decreases in summer. The majority of patients we observed had lower trans-sialidase activity in summer as compared with winter period. Thereby we could see positive correlation between seasonal fluctuation in coronary heart disease risk and activity of trans-sialidase. The highest activity was registered in December, the lowest one in July, thus desialylation in summer decreases by more than 40%. This fact may explain seasonal variations in cardiovascular disease risk. MS100 CORRELATION BETWEEN LAG TIME OF LDL TO IN VITRO OXIDATION AND IN VIVO OX-LDL IN THE PATIENTS WITH CAD H. Nayeri1 , G.A. Naderi2 , S. Asgary2 , F. Pakmehr3 , M. Sadeghi2 , E. Javadi4 . 1 Islamic Azad University, Falavarjan Branch, Falavarjan, 2 Isfahan Cardiovascular Research Center, Isfahan University of Medical Sciences, Isfahan, 3 Food and Drug Department, Isfahan University of Medical Sciences, Iran, 4 Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Background: The oxidative modification of LDL is believed to play an important role in the development of atherosclerosis. Thus, measurement of plasma oxidized LDL (OX-LDL) is essential for atherosclerotic diseases, for investigating its relevance to atherosclerotic diseases. We aimed to assess the OX-LDL in patients with coronary artery disease and correlation between serum OX-LDL and in vitro susceptibility of LDL to oxidation. Methods: Subjects of the study were selected from patients who undergone angiography (42 patients with coronary artery disease and 40 controls without any evidence of CAD). The susceptibility of LDL to in vitro oxidation was assessed with the addition of a CuSO4 solution. The lag time, propagation rate and maximal diene calculated from the oxidation curve. Biochemical factors were measured in these subjects. SPSS version15.5 was used to analyze the data, P- value under 0.05 was considered to be significant.
Memory Stick Presentations
Results: The results indicated that the serum OX-LDL concentration was significantly elevated in CAD patients and the lag time was significantly shorter than controls (P < 0.05). These results clearly confirm that LDL from persons with CAD is more susceptible to oxidative modification in vitro than LDL from healthy subjects. The other measured biochemical factors were not significantly different between patients and controls (P > 0.05). Correlation between serum OX-LDL and susceptibility of LDL to in vitro oxidation did not show significant association (P > 0.05). Conclusion: Our findings suggest that a high OX-LDL concentration and a short LDL oxidation lag time might be independent risk factors for CAD. MS101 EVIDENCE OF INCREASED SDLDL PARTICLES DURING ACUTE INFECTION WITH EPSTEIN−BARR VIRUS F. Apostolou1 , I. Gazi1 , V. Saougos1 , A. Tselepis2 , M. Elisaf1 , E. Liberopoulos1 . 1 Dept of Internal Medicine, Medical School, University of Ioannina, 2 Dept of Biochemistry, School of Chemistry, University of Ioannina, Ioannina, Greece Lipid parameters are altered during infection and these changes may be associated with the pathogenesis of atherosclerosis. There are no data on the possible effects of acute infection (infectious mononucleosis, IM) with Epstein– Barr virus (EBV) on serum lipids and lipoproteins. Methods: Serum lipid parameters were determined in patients with IM on diagnosis and 4 months after the resolution of infection and in age- and sex- matched controls. Fasting levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), triglycerides (TG), apolipoproteins (apo) A-I, B, E and lipoprotein (a) [Lp(a)] were measured. Activities of serum paraoxonase 1 (PON1) and lipoprotein-associated phospholipase A2 (Lp-PLA2 ) as well as levels of cytokines [interleukins (IL)-1b, IL-6 and tumor necrosis factor (TNFa)] were also determined. Low-density lipoprotein (LDL) subclass analysis was performed with the Lipoprint LDL System. Results: Twenty-nine patients (16 male, aged 24.3±14.6 years) and 20 controls were included. TC, HDL-C, LDL-C and apoA-I levels were decreased at baseline whereas apoB/apoA-I ratio and TG levels were increased compared with 4 months after the resolution of IM. At baseline, increases in inflammatory markers (IL-1b, IL-6, TNFa), in sdLDL-C levels and in Lp-PLA2 activity were noticed while LDL particle size was decreased. The levels of lipids and lipoproteins as well as Lp-PLA2 activity of IM patients 4 months after the resolution of the infection were comparable to the corresponding values of the control population. Conclusions: IM is associated with atherogenic changes in serum lipids and lipoproteins. These alterations are fully restored 4 months after the resolution of the infection. MS102 LIPIDS-INDEPENDENT EFFECTS ON BRAIN ISCHEMIC STROKES M. Talebi, V. Ehsasat, S.A. Sadredini, A. Taheragdam. Neurology, Neurosciences Research Center of Tabriz University − Medical Sciences, Tabriz, Iran Introduction: The main mechanism leading ischemia, especially in coronary disease is atherosclerosis. But, there is little consensus that atherosclerosis is main risk factor of brain ischemia (stroke). The role of hyperlipidemia in introducing of coronary disease has been demonstrated, but not in brain vessels. The aim of this study is the assessment of lipids-independed effects on brain ischemic strokes. Methods and Materials: This study performed as case–control study in 40 patients with ischemic strokes and 40 healthy people by comparing the levels of total cholesterol, LDL, HDL, and triglycerides. The exclusion criteria was: The history of hypertension, smoking, cardioembolic strokes, diabetes, disease of the heart, hypo and hyperthyroidism, renal insufficiency, colagenvascular disease and recent use of statins or fibrates. Results: Total cholesterol level was not differing significantly between cases and controls. HDL level was higher significantly in control than cases and HDL level less than 30 mg/dl was associated with the significant increases of the risk of ischemic strokes. About LDL the results show that its level was significantly higher in cases than controls, but despite this difference, LDL levels more than 100 mg/dl was not associated with increase in risk of ischemic strokes. The level of blood triglyceride in patients was lesser than controls but the difference was not significant. Conclusion: This study shows that low levels of serum HDL and high serum level of LDL are two main risk factors in ischemic strokes. The levels of triglyceride and total cholesterol are not associated with the risk of stroke. Keywords: Ischemic strokes, Blood lipids, CholesteroL.
Atherosclerosis Supplements 11, no. 2 (2010) 109–222
Conclusion: The study demonstrates the efficacy of dietary n-3 PUFA to change the pro-atherogenic LDL phenotype to less atherogenic in patients with MetS. Supported by the FW6 EU LIPGENE. MS98 CHOLESTEROL-LOWERING EFFECT OF FORMALDEHYDE INJECTIONS IN SUPER-LOW DOSES E. Tereshina1,2 , S. Ivanenko2 . 1 Russian Clinical and Scientific Center of Gerontology, Moscow, Russia, 2 World Wide Medical Assistance, Zug, Switzerland Formaldehyde (FH) effects on tumor regression and tuberculosis recovery and protection have been registered in animals. Nonspecific homeostatic ways of FM action in the living organism were supposed. So, it was interesting to evaluate FM effect on the cholesterol homeostasis in humans. Toxicity of FM was assessed on 56 outbred ICR mice. In human studies a group of 12 volunteers with various indices of total cholesterol (TC) content (4.3−6.8 mmol/L) was formed. FM was injected i.m. in super low doses for five times with intervals at 7, 21, 30 and 60 days. Samples of blood were taken at 7, 28, 35 and 65 days from the beginning of the experiment. In 8 cases stable decrease in the TC content was achieved at 35 day, in 4 cases − at 7 day. In the whole group final indices of TC were 4.6−2.8 mmol/L. Within cells FM is a substrate for catalase that is located in a peroxisome and involved in the oxidation of fatty acids and synthesis of cholesterol and bile acids. At the very low values of substrate catalase reaction rate increases very rapidly. Modulating effect of FM may appear in gluthation formation and action as they both depend on CH3 group translocations. Thus, FM in super low doses may influence cholesterol homeostasis through modulation of intracellular red/ox potential. MS99 SEASONAL DIFFERENCES IN TRANS-SIALIDASES ACTIVITY E. Orekhova1 , V. Myasoedova1 , I. Sobenin2 , A. Orekhov1 . 1 Inst for Atherosclerosis Res, 2 Inst for General Pathology and Pathophysiology, Moscow, Russia We have earlier found that human blood contains a soluble enzyme, belonging by its donor-acceptor properties to trans-sialidases. Incubation of low density lipoprotein (LDL) with trans-sialidase results in a loss of sialic acid from lipoprotein particles. Desialylated low density lipoprotein induces cholesterol esters accumulation, the main process accompanying atherosclerosis development. Lipoprotein-deficient serum obtained by ultracentrifugation was used for isolation desialylating enzyme by the affinity chromatography. Serum sample was applied on column with a-2,8-Neu5Ac-sepharose. After washing, sorbentbound protein fraction was eluted with 50 mM sialic acid. Electrophoresis of eluted fraction under denaturating conditions revealed the presence of only one protein band with molecular weight about 67 kDa. Activity of this enzyme was measured by transfer of [H3 ] labeled sialic acid from immobilized donor to soluble acceptor. We revealed seasonal differences in trans-sialidases activity. It is well known that coronary heart disease risk decreases in summer. The majority of patients we observed had lower trans-sialidase activity in summer as compared with winter period. Thereby we could see positive correlation between seasonal fluctuation in coronary heart disease risk and activity of trans-sialidase. The highest activity was registered in December, the lowest one in July, thus desialylation in summer decreases by more than 40%. This fact may explain seasonal variations in cardiovascular disease risk. MS100 CORRELATION BETWEEN LAG TIME OF LDL TO IN VITRO OXIDATION AND IN VIVO OX-LDL IN THE PATIENTS WITH CAD H. Nayeri1 , G.A. Naderi2 , S. Asgary2 , F. Pakmehr3 , M. Sadeghi2 , E. Javadi4 . 1 Islamic Azad University, Falavarjan Branch, Falavarjan, 2 Isfahan Cardiovascular Research Center, Isfahan University of Medical Sciences, Isfahan, 3 Food and Drug Department, Isfahan University of Medical Sciences, Iran, 4 Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Background: The oxidative modification of LDL is believed to play an important role in the development of atherosclerosis. Thus, measurement of plasma oxidized LDL (OX-LDL) is essential for atherosclerotic diseases, for investigating its relevance to atherosclerotic diseases. We aimed to assess the OX-LDL in patients with coronary artery disease and correlation between serum OX-LDL and in vitro susceptibility of LDL to oxidation. Methods: Subjects of the study were selected from patients who undergone angiography (42 patients with coronary artery disease and 40 controls without any evidence of CAD). The susceptibility of LDL to in vitro oxidation was assessed with the addition of a CuSO4 solution. The lag time, propagation rate and maximal diene calculated from the oxidation curve. Biochemical factors were measured in these subjects. SPSS version15.5 was used to analyze the data, P- value under 0.05 was considered to be significant.
Memory Stick Presentations
Results: The results indicated that the serum OX-LDL concentration was significantly elevated in CAD patients and the lag time was significantly shorter than controls (P < 0.05). These results clearly confirm that LDL from persons with CAD is more susceptible to oxidative modification in vitro than LDL from healthy subjects. The other measured biochemical factors were not significantly different between patients and controls (P > 0.05). Correlation between serum OX-LDL and susceptibility of LDL to in vitro oxidation did not show significant association (P > 0.05). Conclusion: Our findings suggest that a high OX-LDL concentration and a short LDL oxidation lag time might be independent risk factors for CAD. MS101 EVIDENCE OF INCREASED SDLDL PARTICLES DURING ACUTE INFECTION WITH EPSTEIN−BARR VIRUS F. Apostolou1 , I. Gazi1 , V. Saougos1 , A. Tselepis2 , M. Elisaf1 , E. Liberopoulos1 . 1 Dept of Internal Medicine, Medical School, University of Ioannina, 2 Dept of Biochemistry, School of Chemistry, University of Ioannina, Ioannina, Greece Lipid parameters are altered during infection and these changes may be associated with the pathogenesis of atherosclerosis. There are no data on the possible effects of acute infection (infectious mononucleosis, IM) with Epstein– Barr virus (EBV) on serum lipids and lipoproteins. Methods: Serum lipid parameters were determined in patients with IM on diagnosis and 4 months after the resolution of infection and in age- and sex- matched controls. Fasting levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), triglycerides (TG), apolipoproteins (apo) A-I, B, E and lipoprotein (a) [Lp(a)] were measured. Activities of serum paraoxonase 1 (PON1) and lipoprotein-associated phospholipase A2 (Lp-PLA2 ) as well as levels of cytokines [interleukins (IL)-1b, IL-6 and tumor necrosis factor (TNFa)] were also determined. Low-density lipoprotein (LDL) subclass analysis was performed with the Lipoprint LDL System. Results: Twenty-nine patients (16 male, aged 24.3±14.6 years) and 20 controls were included. TC, HDL-C, LDL-C and apoA-I levels were decreased at baseline whereas apoB/apoA-I ratio and TG levels were increased compared with 4 months after the resolution of IM. At baseline, increases in inflammatory markers (IL-1b, IL-6, TNFa), in sdLDL-C levels and in Lp-PLA2 activity were noticed while LDL particle size was decreased. The levels of lipids and lipoproteins as well as Lp-PLA2 activity of IM patients 4 months after the resolution of the infection were comparable to the corresponding values of the control population. Conclusions: IM is associated with atherogenic changes in serum lipids and lipoproteins. These alterations are fully restored 4 months after the resolution of the infection. MS102 LIPIDS-INDEPENDENT EFFECTS ON BRAIN ISCHEMIC STROKES M. Talebi, V. Ehsasat, S.A. Sadredini, A. Taheragdam. Neurology, Neurosciences Research Center of Tabriz University − Medical Sciences, Tabriz, Iran Introduction: The main mechanism leading ischemia, especially in coronary disease is atherosclerosis. But, there is little consensus that atherosclerosis is main risk factor of brain ischemia (stroke). The role of hyperlipidemia in introducing of coronary disease has been demonstrated, but not in brain vessels. The aim of this study is the assessment of lipids-independed effects on brain ischemic strokes. Methods and Materials: This study performed as case–control study in 40 patients with ischemic strokes and 40 healthy people by comparing the levels of total cholesterol, LDL, HDL, and triglycerides. The exclusion criteria was: The history of hypertension, smoking, cardioembolic strokes, diabetes, disease of the heart, hypo and hyperthyroidism, renal insufficiency, colagenvascular disease and recent use of statins or fibrates. Results: Total cholesterol level was not differing significantly between cases and controls. HDL level was higher significantly in control than cases and HDL level less than 30 mg/dl was associated with the significant increases of the risk of ischemic strokes. About LDL the results show that its level was significantly higher in cases than controls, but despite this difference, LDL levels more than 100 mg/dl was not associated with increase in risk of ischemic strokes. The level of blood triglyceride in patients was lesser than controls but the difference was not significant. Conclusion: This study shows that low levels of serum HDL and high serum level of LDL are two main risk factors in ischemic strokes. The levels of triglyceride and total cholesterol are not associated with the risk of stroke. Keywords: Ischemic strokes, Blood lipids, CholesteroL.