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MS470 MIDTERM FOLLOW UP OF ARTERIAL CORONARY BYPASS GRAFTING USING THE RADIAL ARTERY VERSUS BILATERAL INTERNAL THORACIC ARTERIES
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Atherosclerosis Supplements 11, no. 2 (2010) 109–222
and an increase of CD11c in those with DM. In conclusion, the increase in PMN cytosolic Ca2+ content and the expression of PMN integrins found in VAD patients are markers of PMN activation; the investigation on adhesion molecules has potential therapeutical implications. MS466 THE ROLE OF NATURAL PROANTHOCYANIDINS ON THE IMPROVEMENT OF INFLAMMATORY MARKERS IN DIABETIC RATS 2 M. Badescu1 , R. Furnicaˇ 1 , L. Badescu ˇ , R. Lupu¸soru1 , M. Ciocoiu1 . 1 Pathophysiology, 2 Cell and Molecular Biology, University of Medicine and Pharmacy ‘G.T. Popa’ of Iasi, Iasi, Romania Object of study: The understanding of the concept of inflammation in diabetes-accelerated atherosclerosis can be used practically to predict future cardiovascular risk by evaluating inflammatory biomarkers and to design clinical trials making inflammation as a therapeutic target. Plant-derived proanthocyanidins may exert beneficial effects on diabetes mellitus, in part, because of their antioxidant properties. Method: The benefits of the proanthocyanidins extracted from the black grapes seeds (BGSP), have been shown by using as experimental model the streptozotocin-induced diabetes mellitus on the Wistar white rats. The streptozotocin was administered in a single dose of 60 mg/kg body mass, intraperitoneal. The vegetal proanthocyanidins (BGSP) were administered under the form of water solution, in a dose of 0.028 g/kg body mass, p.o. (through tube feeding), every two days, for a period of 12 weeks. Results: Polyphenols administered to STZ rats reduced blood glucose concentration more that 45% after three weeks of treatment. Interleukin 1beta levels were significantly increased in diabetic compared to witness group. After the polyphenols administration, the values decreased. Regarding the levels of ceruloplasmin (p < 0.001), fibrinogen (p < 0.001) and uric acid (p < 0.01), similar findings were noted. Histologically, in pancreatic and hepatic tissue from group DM+P, there were observed degenerative and inflammatory phenomena associated with regenerative phenomena. Conclusions: The proanthocyanidins extract, with a flavonoidic content of 30.8% in rutoside units out of which 18.3% are antocyans, improves significantly (p < 0.001) the inflamatory phenomena and have a protective effect against diabetes microangiopathy. Keywords: Diabetes mellitus, grape seeds, proanthocyanidins. MS467 OXIDATIVE STRESS MODULATION IN CARDIOVASCULAR DIABETIC DAMAGES BY POLYPHENOLS SUPPLEMENTATION 2 1 1 M. Ciocoiu1 , C. Badescu ˇ , C. Boldea1 , M. Badescu ˇ . Pathophysiology, 2 Internal Medicine, University of Medicine and Pharmacy ‘G.T. Popa’ of Iasi, Iasi, Romania Introduction: The present study concentrates on demonstrating the biological active characteristics of polyphenols extracted from the Sambucus nigra fruits. Material and Method: We used 4 groups of Wistar rats: Group I (W) − witness group; Group II (DM) − experimental induced diabetes with streptozotocin 55 mg/kbw.; Group III (P) − received a natural polyphenols solution, 0.045 mg/kbw, for 16 weeks; Group IV (DM+P) − received polyphenolic extract, in the same dose as group III, for 4 weeks before the induced diabetes and then for 12 weeks after the induced diabetes. Results: Mean hyperglycemia in rats suffering from diabetes and without polyphenolic protection increased progressively. Due to the polyphenolic protection, the serum activity of glutathione peroxidase is more intense for the DM+P group as compared to the DM group. The serum activity of superoxide dismutase for the diabetic group has significantly low values as compared to the ones registered for W and DM+P groups. The obtained results highlight a significant improvement (p < 0.001) in the antioxidative capacity of the serum in diabetic rats treated with natural polyphenols, bringing back to normal the concentration of reduced glutathione, as well as an important decrease in the serum concentration of malondialdehyde, thus proving an important decrease of the lipidic peroxides in serum. Conclusions: The polyphenol-enriched extract from Sambucus nigra do reduce the lipids peroxides, do neutralize the lipid peroxil radicals, and by this may prevent the long term cardiovascular consequences of experimental diabetes. MS468 NONPHARMACOLOGICAL TREATMENT OF OBESITY IN PATIENTS WITH METABOLIC SYNDROME P. Hlubik1 , H. Stritecka1 , J. Hlubik2 . 1 Military Hygiene, University of Defence, Hradec Kralove, 2 Gerstner Laboratory, Czech Technical University, Prague, Czech Republic According to the data of WHO Risk of development of non-infectious diseases (obesity, hypertension, diabetes mellitus II type . . . ) of mass occurrence correlates with the age of monitored individuals. Nowadays it is noted that not only the life-expectancy is prolonging, but also the number of individuals in higher age groups is increasing in the whole Europe including the Czech Republic. Especially obesity partakes of risk increase of metabolic diseases:
Memory Stick Presentations
insulin resistance, small dense oxide LDL, higher level of TAG and lower level of HDL . . . The aim of study was evaluate effect of dietary supplement to body weight reduction. As test drug was used 1 tablet of Sternax (50 mg dry extract from leuzey, 100 mg caffeine) per day during free month’s therapy with dietary restriction and increasing of physical activity, too. Measurement of body composition, body weight, waist and lipid profile, GLU and uric acid was done at beginning and after three months. Change of anthropometrical parameters was statistical significant bud change o f biochemical parameters (TCHOL, HDL, LDL, TAG, GLU, uric acid) was statistically non-significant. Using dietary supplements to be one of the ways in body weight reduction, but must be always physical activity and suitable diet supplemented. Supported by MO FVZ 0000502 MS469 ASSOCIATION BETWEEN NEWLY ONSET ORAL ANTICOAGULANT TREATMENT WITH PHENPROCOUMON AND SERUM LEVELS OF THE VITAMIN-K DEPENDENT CALCIFICATION INHIBITOR MATRIX GLA-PROTEIN R. Koos1 , V. Brandenburg1 , N. Marx1 , L. Schurgers2 . 1 Department of Cardiology, Medical Faculty, RWTH Aachen University, Aachen, Germany, 2 Cardiovascular Research Institute and VitaK, Maastricht University, Maastricht, The Netherlands Introduction: Matrix Gla protein (MGP) is a vitamin K-dependent protein. Recently, the uncarboxylated (inactive,uc) form of MGP was associated with vascular tissue calcification. We aimed to investigate the relationship between a newly initiated oral anticoagulant (OAC) treatment with the Vitamin-K antagonist phenprocoumon and serum levels of uncarboxylated MGP compared to a control group with aspirine therapy. Methods: We performed a prospective study in 43 non-dialyzed patients (mean age 61±10 years) with newly initiated OAC-therapy with phenprocoumon (n = 21) or aged-matched subjects with aspirine therapy (n = 22). In all patients circulating ucMGP serum levels were measured at baseline as well as after 1 month and 3 months follow-up. Results: At baseline no significant difference in ucMGP-levels between patients with newly initiated OAC-therapy (1003±526pM) and control subjects with aspirine therapy (842±214pM, p = 0.40) was detected. At 1 month follow-up, patients with OAC-therapy demonstrated significantly increased ucMGP-levels (1904±402pM) compared to the aspirine group (628±223pM, p < 0.001). At 3 months follow-up, a further increase in ucMGP levels in patients with OACtreatment (2316±344pM) compared to the aspirine group (685±378, p < 0.001) as well as to study inclusion (1003±526pM, p = 0.003) was observed. Conclusion: The present study suggests that newly onset OAC-therapy with phenprocoumon is associated with increased serum levels of inactive,uc-MGP indicating calcification inhibitor deficiency compared to a reference population with aspirine therapy. Long-term follow-up data are needed to evaluate if newly onset OAC-therapy may be linked to an increased progression of coronary and valvular calcification. MS470 MIDTERM FOLLOW UP OF ARTERIAL CORONARY BYPASS GRAFTING USING THE RADIAL ARTERY VERSUS BILATERAL INTERNAL THORACIC ARTERIES J. Schonebeck, ¨ B. Reiter, O. Haye, D. Bohm, ¨ F. Wagner, H. Reichenspurner. ¨ KLinik fur ¨ Herzchirurgie, Universitares Herzzentrum Hamburg/Eppendorf, Hamburg, Germany Objective: Arterial revascularisation using bilateral internal thoracic arteries (BITA) vs unilateral internal thoracic artery (LITA) and radial artery (RA) were compared retrospectively in a case-matched study. Methods: 568 patients from January 2003 to December 2005 received either BITA (group A, n = 116patients) or LITA and RA (group B, n = 452patients). Patients were identified from our surgical database. Patients and referring cardiologists were interviewed by written questionnaires. Results: Significant differences were found in mean age 62.0±8.9 vs.64.4±8.6years (p < 0.05), Body Mass Index 26.3±2.9 vs. 28.2±4.5 (p < 0.05), Euroscore 3.9±3.8 vs. 4.6±5.5 (p < 0.05) and D. mellitus 83.6% vs. 71% (p < 0.001) (group A vs. B respectively). No significant differences were found in rethoracotomy due to bleeding, myocardial infarction and low cardiac output. Mean follow up was 50 months with an overall mortality due to cardiac events of 6.3% group A vs. 7.4% group B. Angiography was performed in 12.5% vs. 16% (p > 0.5) (group A vs. B respectively) due to angina (33%), NYHA class deterioration (33%) and without reliable reasons (33%). Freedom from angina was 87% group A and 84% group B (p > 0.5). In 60% of angiographies in group A and 40% in group B no bypass pathology could be found, occlusions of arterial grafts were provable in 25 patients (19RA) in group B vs. 3 patients (3RIMA) in group A (p < 0.01). Conclusions: Both groups showed excellent clinical results in term of freedom of angina and cardiac events. There was a significant trend to a higher bypass occlusion rate in RA group.
Atherosclerosis Supplements 11, no. 2 (2010) 109–222
and an increase of CD11c in those with DM. In conclusion, the increase in PMN cytosolic Ca2+ content and the expression of PMN integrins found in VAD patients are markers of PMN activation; the investigation on adhesion molecules has potential therapeutical implications. MS466 THE ROLE OF NATURAL PROANTHOCYANIDINS ON THE IMPROVEMENT OF INFLAMMATORY MARKERS IN DIABETIC RATS 2 M. Badescu1 , R. Furnicaˇ 1 , L. Badescu ˇ , R. Lupu¸soru1 , M. Ciocoiu1 . 1 Pathophysiology, 2 Cell and Molecular Biology, University of Medicine and Pharmacy ‘G.T. Popa’ of Iasi, Iasi, Romania Object of study: The understanding of the concept of inflammation in diabetes-accelerated atherosclerosis can be used practically to predict future cardiovascular risk by evaluating inflammatory biomarkers and to design clinical trials making inflammation as a therapeutic target. Plant-derived proanthocyanidins may exert beneficial effects on diabetes mellitus, in part, because of their antioxidant properties. Method: The benefits of the proanthocyanidins extracted from the black grapes seeds (BGSP), have been shown by using as experimental model the streptozotocin-induced diabetes mellitus on the Wistar white rats. The streptozotocin was administered in a single dose of 60 mg/kg body mass, intraperitoneal. The vegetal proanthocyanidins (BGSP) were administered under the form of water solution, in a dose of 0.028 g/kg body mass, p.o. (through tube feeding), every two days, for a period of 12 weeks. Results: Polyphenols administered to STZ rats reduced blood glucose concentration more that 45% after three weeks of treatment. Interleukin 1beta levels were significantly increased in diabetic compared to witness group. After the polyphenols administration, the values decreased. Regarding the levels of ceruloplasmin (p < 0.001), fibrinogen (p < 0.001) and uric acid (p < 0.01), similar findings were noted. Histologically, in pancreatic and hepatic tissue from group DM+P, there were observed degenerative and inflammatory phenomena associated with regenerative phenomena. Conclusions: The proanthocyanidins extract, with a flavonoidic content of 30.8% in rutoside units out of which 18.3% are antocyans, improves significantly (p < 0.001) the inflamatory phenomena and have a protective effect against diabetes microangiopathy. Keywords: Diabetes mellitus, grape seeds, proanthocyanidins. MS467 OXIDATIVE STRESS MODULATION IN CARDIOVASCULAR DIABETIC DAMAGES BY POLYPHENOLS SUPPLEMENTATION 2 1 1 M. Ciocoiu1 , C. Badescu ˇ , C. Boldea1 , M. Badescu ˇ . Pathophysiology, 2 Internal Medicine, University of Medicine and Pharmacy ‘G.T. Popa’ of Iasi, Iasi, Romania Introduction: The present study concentrates on demonstrating the biological active characteristics of polyphenols extracted from the Sambucus nigra fruits. Material and Method: We used 4 groups of Wistar rats: Group I (W) − witness group; Group II (DM) − experimental induced diabetes with streptozotocin 55 mg/kbw.; Group III (P) − received a natural polyphenols solution, 0.045 mg/kbw, for 16 weeks; Group IV (DM+P) − received polyphenolic extract, in the same dose as group III, for 4 weeks before the induced diabetes and then for 12 weeks after the induced diabetes. Results: Mean hyperglycemia in rats suffering from diabetes and without polyphenolic protection increased progressively. Due to the polyphenolic protection, the serum activity of glutathione peroxidase is more intense for the DM+P group as compared to the DM group. The serum activity of superoxide dismutase for the diabetic group has significantly low values as compared to the ones registered for W and DM+P groups. The obtained results highlight a significant improvement (p < 0.001) in the antioxidative capacity of the serum in diabetic rats treated with natural polyphenols, bringing back to normal the concentration of reduced glutathione, as well as an important decrease in the serum concentration of malondialdehyde, thus proving an important decrease of the lipidic peroxides in serum. Conclusions: The polyphenol-enriched extract from Sambucus nigra do reduce the lipids peroxides, do neutralize the lipid peroxil radicals, and by this may prevent the long term cardiovascular consequences of experimental diabetes. MS468 NONPHARMACOLOGICAL TREATMENT OF OBESITY IN PATIENTS WITH METABOLIC SYNDROME P. Hlubik1 , H. Stritecka1 , J. Hlubik2 . 1 Military Hygiene, University of Defence, Hradec Kralove, 2 Gerstner Laboratory, Czech Technical University, Prague, Czech Republic According to the data of WHO Risk of development of non-infectious diseases (obesity, hypertension, diabetes mellitus II type . . . ) of mass occurrence correlates with the age of monitored individuals. Nowadays it is noted that not only the life-expectancy is prolonging, but also the number of individuals in higher age groups is increasing in the whole Europe including the Czech Republic. Especially obesity partakes of risk increase of metabolic diseases:
Memory Stick Presentations
insulin resistance, small dense oxide LDL, higher level of TAG and lower level of HDL . . . The aim of study was evaluate effect of dietary supplement to body weight reduction. As test drug was used 1 tablet of Sternax (50 mg dry extract from leuzey, 100 mg caffeine) per day during free month’s therapy with dietary restriction and increasing of physical activity, too. Measurement of body composition, body weight, waist and lipid profile, GLU and uric acid was done at beginning and after three months. Change of anthropometrical parameters was statistical significant bud change o f biochemical parameters (TCHOL, HDL, LDL, TAG, GLU, uric acid) was statistically non-significant. Using dietary supplements to be one of the ways in body weight reduction, but must be always physical activity and suitable diet supplemented. Supported by MO FVZ 0000502 MS469 ASSOCIATION BETWEEN NEWLY ONSET ORAL ANTICOAGULANT TREATMENT WITH PHENPROCOUMON AND SERUM LEVELS OF THE VITAMIN-K DEPENDENT CALCIFICATION INHIBITOR MATRIX GLA-PROTEIN R. Koos1 , V. Brandenburg1 , N. Marx1 , L. Schurgers2 . 1 Department of Cardiology, Medical Faculty, RWTH Aachen University, Aachen, Germany, 2 Cardiovascular Research Institute and VitaK, Maastricht University, Maastricht, The Netherlands Introduction: Matrix Gla protein (MGP) is a vitamin K-dependent protein. Recently, the uncarboxylated (inactive,uc) form of MGP was associated with vascular tissue calcification. We aimed to investigate the relationship between a newly initiated oral anticoagulant (OAC) treatment with the Vitamin-K antagonist phenprocoumon and serum levels of uncarboxylated MGP compared to a control group with aspirine therapy. Methods: We performed a prospective study in 43 non-dialyzed patients (mean age 61±10 years) with newly initiated OAC-therapy with phenprocoumon (n = 21) or aged-matched subjects with aspirine therapy (n = 22). In all patients circulating ucMGP serum levels were measured at baseline as well as after 1 month and 3 months follow-up. Results: At baseline no significant difference in ucMGP-levels between patients with newly initiated OAC-therapy (1003±526pM) and control subjects with aspirine therapy (842±214pM, p = 0.40) was detected. At 1 month follow-up, patients with OAC-therapy demonstrated significantly increased ucMGP-levels (1904±402pM) compared to the aspirine group (628±223pM, p < 0.001). At 3 months follow-up, a further increase in ucMGP levels in patients with OACtreatment (2316±344pM) compared to the aspirine group (685±378, p < 0.001) as well as to study inclusion (1003±526pM, p = 0.003) was observed. Conclusion: The present study suggests that newly onset OAC-therapy with phenprocoumon is associated with increased serum levels of inactive,uc-MGP indicating calcification inhibitor deficiency compared to a reference population with aspirine therapy. Long-term follow-up data are needed to evaluate if newly onset OAC-therapy may be linked to an increased progression of coronary and valvular calcification. MS470 MIDTERM FOLLOW UP OF ARTERIAL CORONARY BYPASS GRAFTING USING THE RADIAL ARTERY VERSUS BILATERAL INTERNAL THORACIC ARTERIES J. Schonebeck, ¨ B. Reiter, O. Haye, D. Bohm, ¨ F. Wagner, H. Reichenspurner. ¨ KLinik fur ¨ Herzchirurgie, Universitares Herzzentrum Hamburg/Eppendorf, Hamburg, Germany Objective: Arterial revascularisation using bilateral internal thoracic arteries (BITA) vs unilateral internal thoracic artery (LITA) and radial artery (RA) were compared retrospectively in a case-matched study. Methods: 568 patients from January 2003 to December 2005 received either BITA (group A, n = 116patients) or LITA and RA (group B, n = 452patients). Patients were identified from our surgical database. Patients and referring cardiologists were interviewed by written questionnaires. Results: Significant differences were found in mean age 62.0±8.9 vs.64.4±8.6years (p < 0.05), Body Mass Index 26.3±2.9 vs. 28.2±4.5 (p < 0.05), Euroscore 3.9±3.8 vs. 4.6±5.5 (p < 0.05) and D. mellitus 83.6% vs. 71% (p < 0.001) (group A vs. B respectively). No significant differences were found in rethoracotomy due to bleeding, myocardial infarction and low cardiac output. Mean follow up was 50 months with an overall mortality due to cardiac events of 6.3% group A vs. 7.4% group B. Angiography was performed in 12.5% vs. 16% (p > 0.5) (group A vs. B respectively) due to angina (33%), NYHA class deterioration (33%) and without reliable reasons (33%). Freedom from angina was 87% group A and 84% group B (p > 0.5). In 60% of angiographies in group A and 40% in group B no bypass pathology could be found, occlusions of arterial grafts were provable in 25 patients (19RA) in group B vs. 3 patients (3RIMA) in group A (p < 0.01). Conclusions: Both groups showed excellent clinical results in term of freedom of angina and cardiac events. There was a significant trend to a higher bypass occlusion rate in RA group.