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MUC8 as a Ciliated Cell Marker in Human Nasal Epithelium
S166 Abstracts
646
Nasolacrimal Reflux - A New Clinical Finding
SUNDAY
N. W. Chang1, T. M. Foss1, T. Y. Chang1, K. C. Chang1, M. J. Barrett2, J. M. Carney1, H. D. Nguyen1; 1Asthma Allergy Centre, Tigard, OR, 2Allergy Department, Kaiser Permanente Northwest Region, Portland, OR. RATIONALE: Nasolacrimal duct reflux has not been previously described in the medical literature. We studied if it occurs. METHODS: Seventeen subjects, 8 with histories of recurrent acute and/or chronic conjunctivitis and 9 controls without such histories were investigated for nasolacrimal reflux. 0.15 ml of 0.5% ophthalmic fluorescein solution was placed into the inferior meatus with the head down at 45. Reflux was determined by wiping the inner corner of the eye with a tissue after several maneuvers: supine with head down, sitting upright blowing the nose gently or vigorously, and with or without one nostril plugged. The tissue was examined with UV light for the presence of fluorescein. Intranasal pressure was measured with a Millar MPC-500 pressure transducer catheter. RESULTS: Among 8 subjects (age 16-61 yrs, mean 33.1, 3M, 5F) with a history of acute and/or chronic conjunctivitis, 6 had reflux; whereas in 9 controls (age 15-52 yrs, mean 33.4, 2M, 7F), 4 had reflux (p=0.20). In subjects with reflux, one had spontaneous reflux occurring with gravity; others required nose blowing. For all subjects, intranasal pressure of 10 subjects with reflux (22.34±22.00mmHg) did not differ from 7 subjects without reflux (19.87±19.82mmHg) (p=0.21). For subjects with reflux, intranasal pressure when reflux occurred (29.75±23.32mmHg) was higher than when no reflux occurred (20.00±23.48mmHg) (p=0.032). CONCLUSIONS: We established that nasolacrimal reflux can occur both in the supine position and with nose-blowing. Future studies will be necessary to clarify if reflux can lead to ocular symptoms. Effect of Nasal Corticosteroids on Adenoidal Hypertrophy in Children with Non-Allergic Rhinitis S. Kung, E. Yousef, S. J. McGeady; Division of Allergy and Immunology, Thomas Jefferson University, Philadelphia, PA. RATIONALE: Nasal corticosteroids (NCS) reduce adenoidal size in children with allergic rhinitis. Our objective is to determine the effects of NCS upon adenoidal hypertrophy in children with non-allergic rhinitis. METHODS: Records of children with non-allergic rhinitis were reviewed. Those with lateral neck x-rays documenting adenoidal hypertrophy were the study population. Subjects were divided into group A, comprising of patients treated with NCS, and group B who received no NCS. The outcomes of treatment were compared. End points included symptomatic improvement, radiological adenoidal reduction and need for surgical resection. Symptoms assessed included snoring, mouth breathing and periods of apnea or restlessness during sleep. RESULTS: 80 patients aged 9 months to 17 years (mean 4 years) fulfilled the entrance criteria. 42/80 (53%) were male. 58/80 (73%) were treated with NCS (group A), and 22/80 received no NCS (group B). Mean duration of therapy was eight months. 32/58 (55%) in group A improved either symptomatically or radiologically compared to 6/22 (27%) in group B (p=0.03). Adenoidectomies were ultimately performed in 21/58 (36%) of group A compared to 10/22 (45%) in group B (p=0.44). Risk factors in group A non-responders were examined. Age, tobacco smoke exposure, presence of sinusitis and history of gastroesophageal disease (GERD) were similar in the responders and non-responders. However, 9/16 (56%) in the responder group received treatment for GERD, compared to 3/14 (21%) in the non-responder group (p=0.05). CONCLUSIONS: This study suggests that NCS significantly improve adenoidal hypertrophy in children with non-allergic rhinitis. Untreated GERD may result in apparent non-responsiveness to nasal steroids.
647
J ALLERGY CLIN IMMUNOL FEBRUARY 2006
648
MUC8 as a Ciliated Cell Marker in Human Nasal Epithelium
C. Kim1,2, J. Yoon1,2; 1Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, REPUBLIC OF KOREA, 2The Airway Mucus Institute, Seoul, REPUBLIC OF KOREA. RATIONALE: To examine the MUC8 mRNA expression patterns according to the mucociliary differentiation of the normal human nasal epithelial (NHNE) cells, and to investigate the localization of the MUC8 proteins in the nasal polyps. METHODS: The passage-2 NHNE cells cultured using an air-liquid interface technique and nasal polyp specimens. On the 2, 7, 14, and 28 day after confluence, the ciliated cells were counted using cytospin slide immunostaining using H6C5 and -tubulin, and the MUC8 mRNA levels were determined using real-time quantitative PCR. After synthesizing the polyclonal anti-MUC8 peptide antibodies, MUC8 immunostaining was preformed using the nasal polyps. The MUC8 mRNA and protein levels were determined with the NHNE cells treated with IL-1 (10 ng/ml for 24 hours) using RT-PCR and Western blot analysis. RESULTS: The increasing pattern of the number of ciliated cells as well as the MUC8 gene expression level with increasing culture time in the NHNE cells was quite similar. MUC8 was expressed in the ciliated cells of the human nasal polyps. The MUC8 protein level as well as the mRNA level was up-regulated as a result of the IL-1 treatment. CONCLUSIONS: This study indicates that the MUC8 protein is expressed in ciliated cells from the human nasal epithelial cells and is upregulated by the IL-1 treatment. These results suggest that the MUC8 gene and protein expression levels might be used as a ciliated cell marker in the human nasal epithelium. The Importance of Specific IgG, IgE Antibodies to Retinal S Protein, Type II Collagen and Alternaria in Idiopathic Central Serous Chorioretinopathy J. C. Muino1, R. Smith2, J. D. Luna2, M. D. Romero3, M. Ferrero3, C. P. Juárez2; 1Medicine III - UHMI N 4 - Hospital Misericordia, Facultad de Ciencias Médicas Universidad Nacional de Córdoba, Cordoba, ARGENTINA, 2Oftalmología, Fundación Ver, Cordoba, ARGENTINA, 3Inmunología, LIIDO, Cordoba, ARGENTINA. RATIONALE: Idiopathic central serous chorioretinopathy (ICSCR) is a poorly understood ocular disorder associated with the use of corticosteroid medication, or adrenergic agents in allergic respiratory diseases, we have examined patients with ICSCR for the presence of specific IgG and IgE to Retinal S, Alternaria antigens and IgG Type II collagen by ELISA. METHODS: We have studied 15 patients with ICSCR 10 males and 5 females, ranged from 25 to 49 years and compared with 15 healthy non atopic volunteers and 15 asthmatic atopic patients, age and sex matched. The atopic condition was defined by the occurrence of asthma, rhinitis and positive skin prick test for two or more relevant aeroallergen. RESULTS: The specific IgG to Retinal protein was positive in 13 of 15 cases in ICSCR, and negative in atopic asthmatic patients and controls. (p<.0001) The specific IgE to Retinal S protein was positive in 6 of 15 cases in ICSCR, and negative in others groups, (p< 0.024). The specific IgG to type II collagen was positive in 11 of 15 of ICSCR and negative in others groups (p<0.0001). The specific IgG to Alternaria was positive in 12 of 15 cases in ICSCR, in 8 of 15 asthmatic patients, and in 1 of 15 controls (p<0.0002).The specific IgE to Alternaria was positive in 11 of 15 cases of ICSCR, in 1 of 15 controls and in 2 of 15 atopic asthmatic patients. (p< 0.001) CONCLUSIONS: These findings suggest that specific IgG and IgE to Alternaria could probably induce, drive or facilitate the ICSCR process.
649
646
Nasolacrimal Reflux - A New Clinical Finding
SUNDAY
N. W. Chang1, T. M. Foss1, T. Y. Chang1, K. C. Chang1, M. J. Barrett2, J. M. Carney1, H. D. Nguyen1; 1Asthma Allergy Centre, Tigard, OR, 2Allergy Department, Kaiser Permanente Northwest Region, Portland, OR. RATIONALE: Nasolacrimal duct reflux has not been previously described in the medical literature. We studied if it occurs. METHODS: Seventeen subjects, 8 with histories of recurrent acute and/or chronic conjunctivitis and 9 controls without such histories were investigated for nasolacrimal reflux. 0.15 ml of 0.5% ophthalmic fluorescein solution was placed into the inferior meatus with the head down at 45. Reflux was determined by wiping the inner corner of the eye with a tissue after several maneuvers: supine with head down, sitting upright blowing the nose gently or vigorously, and with or without one nostril plugged. The tissue was examined with UV light for the presence of fluorescein. Intranasal pressure was measured with a Millar MPC-500 pressure transducer catheter. RESULTS: Among 8 subjects (age 16-61 yrs, mean 33.1, 3M, 5F) with a history of acute and/or chronic conjunctivitis, 6 had reflux; whereas in 9 controls (age 15-52 yrs, mean 33.4, 2M, 7F), 4 had reflux (p=0.20). In subjects with reflux, one had spontaneous reflux occurring with gravity; others required nose blowing. For all subjects, intranasal pressure of 10 subjects with reflux (22.34±22.00mmHg) did not differ from 7 subjects without reflux (19.87±19.82mmHg) (p=0.21). For subjects with reflux, intranasal pressure when reflux occurred (29.75±23.32mmHg) was higher than when no reflux occurred (20.00±23.48mmHg) (p=0.032). CONCLUSIONS: We established that nasolacrimal reflux can occur both in the supine position and with nose-blowing. Future studies will be necessary to clarify if reflux can lead to ocular symptoms. Effect of Nasal Corticosteroids on Adenoidal Hypertrophy in Children with Non-Allergic Rhinitis S. Kung, E. Yousef, S. J. McGeady; Division of Allergy and Immunology, Thomas Jefferson University, Philadelphia, PA. RATIONALE: Nasal corticosteroids (NCS) reduce adenoidal size in children with allergic rhinitis. Our objective is to determine the effects of NCS upon adenoidal hypertrophy in children with non-allergic rhinitis. METHODS: Records of children with non-allergic rhinitis were reviewed. Those with lateral neck x-rays documenting adenoidal hypertrophy were the study population. Subjects were divided into group A, comprising of patients treated with NCS, and group B who received no NCS. The outcomes of treatment were compared. End points included symptomatic improvement, radiological adenoidal reduction and need for surgical resection. Symptoms assessed included snoring, mouth breathing and periods of apnea or restlessness during sleep. RESULTS: 80 patients aged 9 months to 17 years (mean 4 years) fulfilled the entrance criteria. 42/80 (53%) were male. 58/80 (73%) were treated with NCS (group A), and 22/80 received no NCS (group B). Mean duration of therapy was eight months. 32/58 (55%) in group A improved either symptomatically or radiologically compared to 6/22 (27%) in group B (p=0.03). Adenoidectomies were ultimately performed in 21/58 (36%) of group A compared to 10/22 (45%) in group B (p=0.44). Risk factors in group A non-responders were examined. Age, tobacco smoke exposure, presence of sinusitis and history of gastroesophageal disease (GERD) were similar in the responders and non-responders. However, 9/16 (56%) in the responder group received treatment for GERD, compared to 3/14 (21%) in the non-responder group (p=0.05). CONCLUSIONS: This study suggests that NCS significantly improve adenoidal hypertrophy in children with non-allergic rhinitis. Untreated GERD may result in apparent non-responsiveness to nasal steroids.
647
J ALLERGY CLIN IMMUNOL FEBRUARY 2006
648
MUC8 as a Ciliated Cell Marker in Human Nasal Epithelium
C. Kim1,2, J. Yoon1,2; 1Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, REPUBLIC OF KOREA, 2The Airway Mucus Institute, Seoul, REPUBLIC OF KOREA. RATIONALE: To examine the MUC8 mRNA expression patterns according to the mucociliary differentiation of the normal human nasal epithelial (NHNE) cells, and to investigate the localization of the MUC8 proteins in the nasal polyps. METHODS: The passage-2 NHNE cells cultured using an air-liquid interface technique and nasal polyp specimens. On the 2, 7, 14, and 28 day after confluence, the ciliated cells were counted using cytospin slide immunostaining using H6C5 and -tubulin, and the MUC8 mRNA levels were determined using real-time quantitative PCR. After synthesizing the polyclonal anti-MUC8 peptide antibodies, MUC8 immunostaining was preformed using the nasal polyps. The MUC8 mRNA and protein levels were determined with the NHNE cells treated with IL-1 (10 ng/ml for 24 hours) using RT-PCR and Western blot analysis. RESULTS: The increasing pattern of the number of ciliated cells as well as the MUC8 gene expression level with increasing culture time in the NHNE cells was quite similar. MUC8 was expressed in the ciliated cells of the human nasal polyps. The MUC8 protein level as well as the mRNA level was up-regulated as a result of the IL-1 treatment. CONCLUSIONS: This study indicates that the MUC8 protein is expressed in ciliated cells from the human nasal epithelial cells and is upregulated by the IL-1 treatment. These results suggest that the MUC8 gene and protein expression levels might be used as a ciliated cell marker in the human nasal epithelium. The Importance of Specific IgG, IgE Antibodies to Retinal S Protein, Type II Collagen and Alternaria in Idiopathic Central Serous Chorioretinopathy J. C. Muino1, R. Smith2, J. D. Luna2, M. D. Romero3, M. Ferrero3, C. P. Juárez2; 1Medicine III - UHMI N 4 - Hospital Misericordia, Facultad de Ciencias Médicas Universidad Nacional de Córdoba, Cordoba, ARGENTINA, 2Oftalmología, Fundación Ver, Cordoba, ARGENTINA, 3Inmunología, LIIDO, Cordoba, ARGENTINA. RATIONALE: Idiopathic central serous chorioretinopathy (ICSCR) is a poorly understood ocular disorder associated with the use of corticosteroid medication, or adrenergic agents in allergic respiratory diseases, we have examined patients with ICSCR for the presence of specific IgG and IgE to Retinal S, Alternaria antigens and IgG Type II collagen by ELISA. METHODS: We have studied 15 patients with ICSCR 10 males and 5 females, ranged from 25 to 49 years and compared with 15 healthy non atopic volunteers and 15 asthmatic atopic patients, age and sex matched. The atopic condition was defined by the occurrence of asthma, rhinitis and positive skin prick test for two or more relevant aeroallergen. RESULTS: The specific IgG to Retinal protein was positive in 13 of 15 cases in ICSCR, and negative in atopic asthmatic patients and controls. (p<.0001) The specific IgE to Retinal S protein was positive in 6 of 15 cases in ICSCR, and negative in others groups, (p< 0.024). The specific IgG to type II collagen was positive in 11 of 15 of ICSCR and negative in others groups (p<0.0001). The specific IgG to Alternaria was positive in 12 of 15 cases in ICSCR, in 8 of 15 asthmatic patients, and in 1 of 15 controls (p<0.0002).The specific IgE to Alternaria was positive in 11 of 15 cases of ICSCR, in 1 of 15 controls and in 2 of 15 atopic asthmatic patients. (p< 0.001) CONCLUSIONS: These findings suggest that specific IgG and IgE to Alternaria could probably induce, drive or facilitate the ICSCR process.
649