- Email: [email protected]
Multi-site hippocampal subfields reproducibility: A european 3T study
P558 P2-174
Poster Presentations: P2 MULTI-SITE HIPPOCAMPAL SUBFIELDS REPRODUCIBILITY: A EUROPEAN 3T STUDY
Moira Marizzoni1, Flavio Nobili2, Mira Didic3,4, David Bartres5, onknecht7,8, Pierre Payoux9,10, Alberto Beltramello11, Ute Fiedler6, Peter Sch€ Andrea Soricelli12,13, Lucilla Parnetti14, Magda Tsolaki15, Paolo Maria Rossini16,17, Philip Scheltens18,19, Regis Bordet20, Olivier Blin21, Giovanni Battista Frisoni22,23, Jorge Jovicich24 PharmaCog Consortium, 1Laboratory of Epidemiology, Neuroimaging and Telemedicine/ IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy; 2 University of Genoa, Genoa, Italy; 3Service de Neurologie et Neuropsychologie, Marseille, France; 4Aix-Marseille Universite, Marseille, France; 5Universitat de Barcelona and IDIBAPS, Barcelona, Spain; 6 Institutes and Clinics of the University Duisburg-Essen, Essen, Germany; 7 LIFE – Leipzig Research Center for Civilization Diseases, Leipzig, Germany; 8Department of Psychiatry and Psychotherapy, University Hospital Leipzig, Leipzig, Germany; 9Imagerie Cerebrale et Handicaps Neurologiques, Toulouse, France; 10Universite de Toulouse, Toulouse, France; 11General Hospital, Verona, Italy; 12IRCCS SDN, Naples, Italy; 13 University of Naples Parthenope, Naples, Italy; 14University of Perugia, Perugia, Italy; 15Aristotle University of Thessaloniki, Thessaloniki, Greece; 16 Catholic University of Rome, Rome, Italy; 17Policlinic Gemelli, Rome, Italy; 18VU University Medical Center, Amsterdam, Netherlands; 19 Neuroscience Campus Amsterdam, Amsterdam, Netherlands; 20University of Lille Nord de France, Lille, France; 21Aix-Marseille University-CNRS, Marseille, France; 22IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy; 23Memory Clinic and LANVIE - Laboratory of Neuroimaging of Aging, University Hospitals and University of Geneva, Geneva, Switzerland; 24University of Trento, Trento, Italy. Contact e-mail: [email protected] Background: Hippocampal subfields are differentially affected in Alzheimer’s Disease (Hanseeuw BJ, 2011; Lim HK, 2012). However, their volume reproducibility has been poorly investigated. We report the evaluation of the across-session test-retest reproducibility of the hippocampal subfields segmentations derived from Freesurfer in 65 healthy elderly subjects. We compared the subfields reproducibility i) averaging or not two within session T1 images and ii) relative to the whole hippocampus. Methods: Five healthy local volunteers (55-90 ys) were enrolled in 13 3T MRI sites (Siemens, GE, Philips) across Europe and were scanned in two sessions at least a week apart. All analyses were performed using the longitudinal pipeline of Freesurfer v5.1.0 (Reuter M, 2012; Jovicich J, 2013) on the neuGRID platform (https://neugrid4you.eu/). The whole hippocampal volume was extracted from the “aseg.stat” file of Fresurfer. For each site and ROI, volumes reliability was computed evaluating test-retest absolute differences relative to the mean (absolute error) and test-retest spatial reproducibility (DICE). Results: Subfields analysis was focused on: Cornu Ammonis (CA) 1, CA23, CA4-dentate gyrus (DG), subiculum, presubiculum, fimbria and hippocampal fissure. Within session averaging of two T1 images gave a significant improvement in the mean test-retest reproducibility of all hippocampal subfields. Absolute errors across MRI sites was comparable to that found for whole hippocampus (about 2%) for CA2-3, CA4-DG and subiculum, about 5% for CA1 and presubiculum, around 15% for fimbria and hippocampal fissure. The DICE results were in line with the absolute error analysis (excellent for CA2-3, CA4-DG, subiculum, good for CA1 and presubiculum, poor for fimbria and hippocampal fissure). Conclusions: Averaging the within session T1 images allowed to improve the Freesurfer subfields reproducibility at each site. Despite the differences of the 13 MRI scanner configurations we found good and consistent hippocampal subfields reproducibility for CA2-3, CA4DG, subiculum, CA1 and presubiculum. Pharmacog is funded by the EU-FP7 for the Innovative Medicine Initiative (grant 115009).
P2-175
ARE CORTICAL SOURCES OF RESTING STATE EYES-CLOSED ELECTROENCEPHALOGRAPHIC RHYTHMS AN EARLY DIAGNOSTIC MARKER OF ALZHEIMER’S DISEASE?
Claudio Babiloni1, Claudio Del Percio2, Nicola Marzano3, Susanna Cordone3, Giuseppe Noce4, Christina Bagnoli5, Paolo Maria Rossini6, Andrea Soricelli4, Flavio Nobili7, David Bartres8, Olivier Blin9, Pierre Payoux10, Regis Bordet11, Bernhard W. Muller12, Magda Tsolaki13, Lucilla Parnetti14, Ulrich Hegerl15, Juergen Dukart16, Alessandro Bartolino16, Gianluigi Forloni17, Jill Richardson18, Giovanni Battista Frisoni5, Tilman Hensch15, 1Sapienza University of Rome, Rome, Italy; 2IRCSS San Raffaele Pisana, Rome, Italy; 3University of Foggia, Foggia, Italy; 4IRCCS SDN, Naples, Italy; 5IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy; 6Catholic University of Rome, Rome, Italy; 7University of Genoa, Genoa, Italy; 8Universitat de Barcelona and IDIBAPS, Barcelona, Spain; 9Mediterranean Institute of Cognitive Neurosciences, Marseille, France; 10Universite de Toulouse, Toulouse, France; 11Lille 2 University, Lille, France; 12University of Duisburg-Essen, Essen, Germany; 13Aristotle University of Thessaloniki, Thessaloniki, Greece; 14University of Perugia, Perugia, Italy; 15University of Leipzig, Leipzig, Germany; 16Roche, Basel, Switzerland; 17IRCCS Istituto di Ricerche Farmacologiche “Mario Negri”, Milano, Italy; 18 Neurosciences Therapeutic Area, U.K., United Kingdom. Contact e-mail: [email protected] Background: Mild cognitive impairment (MCI) subjects were previ-
ously characterized by abnormal cortical sources of resting state electroencephalographic (EEG) rhythms but it is unclear the specificity of this result with reference to Alzheimer’s disease (AD). To address this issue, in the framework of IMI PharmaCog project (Grant Agreement n 115009, www.pharmacog.org), this study evaluated whether cortical sources of these rhythms are correlated to cerebrospinal fluid b amyloid (CSF Ab) level in amnesic MCI (aMCI) subjects. Methods: The research data (including human biological samples) were sourced ethically and their research use was in accord with the terms of the international ethical standards. Artifact free resting state eyes-closed EEG rhythms (19 electrodes) were recorded in 127 aMCI subjects in the framework of WP5 PharmaCog project. Individual data sets were broken down into those with high CSF Ab level (81 Ab negative, CSF Ab > 550 pg/ml) and those with low CSF Ab level (46 Ab negative, CSF Ab < 550 pg/ml). Cortical sources of global delta (2-4 Hz) and low-frequency alpha (8-10.5 Hz) EEG rhythms were estimated by LORETA package. Results: Compared to the Ab-negative aMCI group, the Ab-positive aMCI group showed higher global cortical sources
Figure 1. Individual values of global normalized cortical (LORETA) sources for resting state eyes-closed in the Ab-negative and Ab-positive aMCI subjects. In particular, the individual values for delta (2-4 Hz) and alpha (8-10.5 Hz) bands are reported.
Poster Presentations: P2 MULTI-SITE HIPPOCAMPAL SUBFIELDS REPRODUCIBILITY: A EUROPEAN 3T STUDY
Moira Marizzoni1, Flavio Nobili2, Mira Didic3,4, David Bartres5, onknecht7,8, Pierre Payoux9,10, Alberto Beltramello11, Ute Fiedler6, Peter Sch€ Andrea Soricelli12,13, Lucilla Parnetti14, Magda Tsolaki15, Paolo Maria Rossini16,17, Philip Scheltens18,19, Regis Bordet20, Olivier Blin21, Giovanni Battista Frisoni22,23, Jorge Jovicich24 PharmaCog Consortium, 1Laboratory of Epidemiology, Neuroimaging and Telemedicine/ IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy; 2 University of Genoa, Genoa, Italy; 3Service de Neurologie et Neuropsychologie, Marseille, France; 4Aix-Marseille Universite, Marseille, France; 5Universitat de Barcelona and IDIBAPS, Barcelona, Spain; 6 Institutes and Clinics of the University Duisburg-Essen, Essen, Germany; 7 LIFE – Leipzig Research Center for Civilization Diseases, Leipzig, Germany; 8Department of Psychiatry and Psychotherapy, University Hospital Leipzig, Leipzig, Germany; 9Imagerie Cerebrale et Handicaps Neurologiques, Toulouse, France; 10Universite de Toulouse, Toulouse, France; 11General Hospital, Verona, Italy; 12IRCCS SDN, Naples, Italy; 13 University of Naples Parthenope, Naples, Italy; 14University of Perugia, Perugia, Italy; 15Aristotle University of Thessaloniki, Thessaloniki, Greece; 16 Catholic University of Rome, Rome, Italy; 17Policlinic Gemelli, Rome, Italy; 18VU University Medical Center, Amsterdam, Netherlands; 19 Neuroscience Campus Amsterdam, Amsterdam, Netherlands; 20University of Lille Nord de France, Lille, France; 21Aix-Marseille University-CNRS, Marseille, France; 22IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy; 23Memory Clinic and LANVIE - Laboratory of Neuroimaging of Aging, University Hospitals and University of Geneva, Geneva, Switzerland; 24University of Trento, Trento, Italy. Contact e-mail: [email protected] Background: Hippocampal subfields are differentially affected in Alzheimer’s Disease (Hanseeuw BJ, 2011; Lim HK, 2012). However, their volume reproducibility has been poorly investigated. We report the evaluation of the across-session test-retest reproducibility of the hippocampal subfields segmentations derived from Freesurfer in 65 healthy elderly subjects. We compared the subfields reproducibility i) averaging or not two within session T1 images and ii) relative to the whole hippocampus. Methods: Five healthy local volunteers (55-90 ys) were enrolled in 13 3T MRI sites (Siemens, GE, Philips) across Europe and were scanned in two sessions at least a week apart. All analyses were performed using the longitudinal pipeline of Freesurfer v5.1.0 (Reuter M, 2012; Jovicich J, 2013) on the neuGRID platform (https://neugrid4you.eu/). The whole hippocampal volume was extracted from the “aseg.stat” file of Fresurfer. For each site and ROI, volumes reliability was computed evaluating test-retest absolute differences relative to the mean (absolute error) and test-retest spatial reproducibility (DICE). Results: Subfields analysis was focused on: Cornu Ammonis (CA) 1, CA23, CA4-dentate gyrus (DG), subiculum, presubiculum, fimbria and hippocampal fissure. Within session averaging of two T1 images gave a significant improvement in the mean test-retest reproducibility of all hippocampal subfields. Absolute errors across MRI sites was comparable to that found for whole hippocampus (about 2%) for CA2-3, CA4-DG and subiculum, about 5% for CA1 and presubiculum, around 15% for fimbria and hippocampal fissure. The DICE results were in line with the absolute error analysis (excellent for CA2-3, CA4-DG, subiculum, good for CA1 and presubiculum, poor for fimbria and hippocampal fissure). Conclusions: Averaging the within session T1 images allowed to improve the Freesurfer subfields reproducibility at each site. Despite the differences of the 13 MRI scanner configurations we found good and consistent hippocampal subfields reproducibility for CA2-3, CA4DG, subiculum, CA1 and presubiculum. Pharmacog is funded by the EU-FP7 for the Innovative Medicine Initiative (grant 115009).
P2-175
ARE CORTICAL SOURCES OF RESTING STATE EYES-CLOSED ELECTROENCEPHALOGRAPHIC RHYTHMS AN EARLY DIAGNOSTIC MARKER OF ALZHEIMER’S DISEASE?
Claudio Babiloni1, Claudio Del Percio2, Nicola Marzano3, Susanna Cordone3, Giuseppe Noce4, Christina Bagnoli5, Paolo Maria Rossini6, Andrea Soricelli4, Flavio Nobili7, David Bartres8, Olivier Blin9, Pierre Payoux10, Regis Bordet11, Bernhard W. Muller12, Magda Tsolaki13, Lucilla Parnetti14, Ulrich Hegerl15, Juergen Dukart16, Alessandro Bartolino16, Gianluigi Forloni17, Jill Richardson18, Giovanni Battista Frisoni5, Tilman Hensch15, 1Sapienza University of Rome, Rome, Italy; 2IRCSS San Raffaele Pisana, Rome, Italy; 3University of Foggia, Foggia, Italy; 4IRCCS SDN, Naples, Italy; 5IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy; 6Catholic University of Rome, Rome, Italy; 7University of Genoa, Genoa, Italy; 8Universitat de Barcelona and IDIBAPS, Barcelona, Spain; 9Mediterranean Institute of Cognitive Neurosciences, Marseille, France; 10Universite de Toulouse, Toulouse, France; 11Lille 2 University, Lille, France; 12University of Duisburg-Essen, Essen, Germany; 13Aristotle University of Thessaloniki, Thessaloniki, Greece; 14University of Perugia, Perugia, Italy; 15University of Leipzig, Leipzig, Germany; 16Roche, Basel, Switzerland; 17IRCCS Istituto di Ricerche Farmacologiche “Mario Negri”, Milano, Italy; 18 Neurosciences Therapeutic Area, U.K., United Kingdom. Contact e-mail: [email protected] Background: Mild cognitive impairment (MCI) subjects were previ-
ously characterized by abnormal cortical sources of resting state electroencephalographic (EEG) rhythms but it is unclear the specificity of this result with reference to Alzheimer’s disease (AD). To address this issue, in the framework of IMI PharmaCog project (Grant Agreement n 115009, www.pharmacog.org), this study evaluated whether cortical sources of these rhythms are correlated to cerebrospinal fluid b amyloid (CSF Ab) level in amnesic MCI (aMCI) subjects. Methods: The research data (including human biological samples) were sourced ethically and their research use was in accord with the terms of the international ethical standards. Artifact free resting state eyes-closed EEG rhythms (19 electrodes) were recorded in 127 aMCI subjects in the framework of WP5 PharmaCog project. Individual data sets were broken down into those with high CSF Ab level (81 Ab negative, CSF Ab > 550 pg/ml) and those with low CSF Ab level (46 Ab negative, CSF Ab < 550 pg/ml). Cortical sources of global delta (2-4 Hz) and low-frequency alpha (8-10.5 Hz) EEG rhythms were estimated by LORETA package. Results: Compared to the Ab-negative aMCI group, the Ab-positive aMCI group showed higher global cortical sources
Figure 1. Individual values of global normalized cortical (LORETA) sources for resting state eyes-closed in the Ab-negative and Ab-positive aMCI subjects. In particular, the individual values for delta (2-4 Hz) and alpha (8-10.5 Hz) bands are reported.