- Email: [email protected]
NCCLS Publishers Bactericidal Testing Guidelines
From the CDC/NIH
CDC/NIH Manual on Laboratory Biosafety, Second Edition, Now Available The second edition of &safety in the Microbiological and Biomedical Laboratories is now available. The first edition of this handbook was developed jointly by the Centers for Dis-
ease Control and the National Institutes of Health in 1984 to provide guidelines in biosafety for use in various laboratory settings. It outlines standard and special microbiologic practices, safety equipment, and facilities that constitute the four biosafety levels. Recommended safe techniques are described in detail for special infectious agents. This 1988 edition is identical in content but has added information on handling of the human immunodeficiency virus or specimens containing this virus in
the laboratory. Requests for copies of this manual (Stock No. 107-04000508-3, $3.75 per copy, prepaid) should be directed to:
serum bactericidal test, a method that is a variation of the NCCLS standard broth microdilution test for susceptibility testing of aerobic bacteria. The document describes the principle of the test, its clinical use, procedures for collecting the patient’s serum, micro- and macrodilution methods for determining serum bactericidal titer, determination and interpretation of endpoints, and quality control. Document M26-P describes dilution and time-kill methods for determining the lethal activity of antimicrobial agents. It also describes procedures for determining minimum bactericidal concentrations by dilution methods, interpretation of endpoints, and quality control.
Both guidelines were written by the NCCLS Subcommittee on Serum Bactericidal Testing, chaired by Charles W. Stratton, M.D., Vanderbilt University. These proposed guidelines are NCCLS’s first recommendations in the area of bactericidal testing. They are intended for broad review of the scope, utility, and methodology. Comments may be sent to NCCLS.
NIWDS 31-lC02 Bethesda, MD 20892 The Superintendent of Documents US Government Printing Office Washington, D.C. 20402
NCCLS News
NCCLS Publishes Bactericidal Testing Guidelines The National Committee for Clinical Laboratory Standards (NCCLS) has published two new documents on bactericidal testing: Methodology for the Serum Bactericidal Test; Proposed Guideline (NCCLS Document M21-P, Vol. 7 No. 1) and Methods for Determining Bactericidal Activity of Antimicrobial Agents; Proposed Guideline
(NCCLS Document M26-P, Vol. 7 No. 2). Document M21-P recommends a uniform method for performing the
General Information Subscription information
Editors
Mary Jane Ferraro Paul A. Granato Josephine A. Morello R. J. Zabransky 0
1989 Elsevier Science Publishing
ISSN 0196-4399 CMNEEJ 11(5)33-40,
Co., Inc.
1989
NCCLS documents are available for $20 a copy ($25 for overseas orders). Payment is requested in advance, and discounts are available for multiplecopy orders. Further details may be obtained from NCCLS, 77 1 E. Lancaster Avenue, Villanova, PA 19085, (215) 525-2435.
can be found inside the front cover.
This newsletter has been registered with the Copyright Clearance Center, Inc. Consent is given for copying articles for personal or internal use, or for the personal or internal use of specific clients. This consent is given on the condition that the copier pay through the Center the per-page fee stated in the code on each page for copying beyond that permitted by the U.S. Copyright Law. If no code appears on an article, the author has not given broad consent to copy, and permission to copy must be obtained directly from the author. This consent does not extend to other kinds of copying, such as for general distribution, resale, advertising and promotional purposes, or for creating new collective works. This newsletter is printed on acid-free paper. Address orders, changes of address, and claims for missing issues to Journals Fultillment Department, Elsevier Science Publishing Co., Inc., 655 Avenue of the Americas, New York, NY 10010. Claims for missing issues can be honored only up to three months for domestic addresses and six months for foreign addresses. Duplicate copies will not be sent to replace ones undelivered due to failure to notify Elsevier of change of address.
Elsevier
Postmaster: Send address changes to Clinical Microbiology Newsletter, Elsevier Science Publishing Co., Inc., 655 Avenue of the Americas, New York, NY 10010. Editorials and letters printed in this newsletter are published for the interest of the readers and do not necessarily reflect the opinions of the editors. Clinical Microbiology Newsletter is abstracted in Tropical Diseases Bulletin and Abstracts on Hygiene and Communicable Diseases.
40
019fl-4799/89/30.00
+ 02.20
0 1989 Elsevier Science Publishing Co., Inc.
Clinical Microbiology
Newsletter
115, 1989
CDC/NIH Manual on Laboratory Biosafety, Second Edition, Now Available The second edition of &safety in the Microbiological and Biomedical Laboratories is now available. The first edition of this handbook was developed jointly by the Centers for Dis-
ease Control and the National Institutes of Health in 1984 to provide guidelines in biosafety for use in various laboratory settings. It outlines standard and special microbiologic practices, safety equipment, and facilities that constitute the four biosafety levels. Recommended safe techniques are described in detail for special infectious agents. This 1988 edition is identical in content but has added information on handling of the human immunodeficiency virus or specimens containing this virus in
the laboratory. Requests for copies of this manual (Stock No. 107-04000508-3, $3.75 per copy, prepaid) should be directed to:
serum bactericidal test, a method that is a variation of the NCCLS standard broth microdilution test for susceptibility testing of aerobic bacteria. The document describes the principle of the test, its clinical use, procedures for collecting the patient’s serum, micro- and macrodilution methods for determining serum bactericidal titer, determination and interpretation of endpoints, and quality control. Document M26-P describes dilution and time-kill methods for determining the lethal activity of antimicrobial agents. It also describes procedures for determining minimum bactericidal concentrations by dilution methods, interpretation of endpoints, and quality control.
Both guidelines were written by the NCCLS Subcommittee on Serum Bactericidal Testing, chaired by Charles W. Stratton, M.D., Vanderbilt University. These proposed guidelines are NCCLS’s first recommendations in the area of bactericidal testing. They are intended for broad review of the scope, utility, and methodology. Comments may be sent to NCCLS.
NIWDS 31-lC02 Bethesda, MD 20892 The Superintendent of Documents US Government Printing Office Washington, D.C. 20402
NCCLS News
NCCLS Publishes Bactericidal Testing Guidelines The National Committee for Clinical Laboratory Standards (NCCLS) has published two new documents on bactericidal testing: Methodology for the Serum Bactericidal Test; Proposed Guideline (NCCLS Document M21-P, Vol. 7 No. 1) and Methods for Determining Bactericidal Activity of Antimicrobial Agents; Proposed Guideline
(NCCLS Document M26-P, Vol. 7 No. 2). Document M21-P recommends a uniform method for performing the
General Information Subscription information
Editors
Mary Jane Ferraro Paul A. Granato Josephine A. Morello R. J. Zabransky 0
1989 Elsevier Science Publishing
ISSN 0196-4399 CMNEEJ 11(5)33-40,
Co., Inc.
1989
NCCLS documents are available for $20 a copy ($25 for overseas orders). Payment is requested in advance, and discounts are available for multiplecopy orders. Further details may be obtained from NCCLS, 77 1 E. Lancaster Avenue, Villanova, PA 19085, (215) 525-2435.
can be found inside the front cover.
This newsletter has been registered with the Copyright Clearance Center, Inc. Consent is given for copying articles for personal or internal use, or for the personal or internal use of specific clients. This consent is given on the condition that the copier pay through the Center the per-page fee stated in the code on each page for copying beyond that permitted by the U.S. Copyright Law. If no code appears on an article, the author has not given broad consent to copy, and permission to copy must be obtained directly from the author. This consent does not extend to other kinds of copying, such as for general distribution, resale, advertising and promotional purposes, or for creating new collective works. This newsletter is printed on acid-free paper. Address orders, changes of address, and claims for missing issues to Journals Fultillment Department, Elsevier Science Publishing Co., Inc., 655 Avenue of the Americas, New York, NY 10010. Claims for missing issues can be honored only up to three months for domestic addresses and six months for foreign addresses. Duplicate copies will not be sent to replace ones undelivered due to failure to notify Elsevier of change of address.
Elsevier
Postmaster: Send address changes to Clinical Microbiology Newsletter, Elsevier Science Publishing Co., Inc., 655 Avenue of the Americas, New York, NY 10010. Editorials and letters printed in this newsletter are published for the interest of the readers and do not necessarily reflect the opinions of the editors. Clinical Microbiology Newsletter is abstracted in Tropical Diseases Bulletin and Abstracts on Hygiene and Communicable Diseases.
40
019fl-4799/89/30.00
+ 02.20
0 1989 Elsevier Science Publishing Co., Inc.
Clinical Microbiology
Newsletter
115, 1989