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Neurotensin and primary hepato-cellular carcinoma
178 NEUROTENSIN AND PRIMARY HEPATO-CELLULAR CARCINOMA Jiang RL, Fu By. Dept of Medicine, The First Affiliated Hospital, China Medical University, Shenyang, 110001, P.R. China The concentration of plasma neurotensin (NT) was measured in 72 patients with primary hepatocellular Carcinoma (PHC) and 46 normal controls by radioimmunoassay. The mean plasma concentration of NT was 12.664-5.89 PM in patients and 14.06+9.71 PM in controls. There was no significant difference between patients and controls. Immunohistochemical studies of NT were carried out in 19 PHC liver tissues and 10 other hepatomas and 10 non- hepatoma liver tissues by staining with ABC method. It was found that 9 of 19 PHC tissues were immunohistochemically positive, while the other two groups were negative except one hepatocholangioma. NT-positive substance was present in the cytoplasm of the cancer cells and absent in the surrounding stroma adjacent to the malignant cells. There was no parallel relationship between immunohistochenlical localization of NT and plasma NT levels. The results indicate that the PHC tissues may contain NT, and the PHC cancer cells are associated with the ectopic production of NT.
ROLES OF GUT HORMONES IN NEGATIVE FEEDBACK REGULATION OF PANCREATIC EXOCRINE SECRETION IN HUMANS. Jin HO, Song CW, Chang TM, Chey WY. The Genesee Hospital, University of Rochester, Rochester, NY 14607, U.S.A. Negative feedback regulation of pancreatic secretion in humans has been shown to be mediated by cholecystokinin (CCK), and there are no studies investigating the effects of other hormones. We studied possible roles of secretin, CCK, neurotensin (NT) and pancreatic polypeptide (PP) on the feedback regulation in 10 healthy fasting humans. A five lumen tube with its distal open tip was positioned in proximal jejunum (PJ) under fluoroscopy, so that gastric juice (via lumen 1) and duodenal contents (via lumen 3) were collected separately by aspiration in 15 min samples while PEG solution was infused into proximal duodenum via lumen 2. An acidified (pH 2.0) 4.25% amino acid meal (AA) mixed with phenol red was infused into PJ via lumen 4 and testing solutions, either 0.1 N NaHCO3 or trypsin 600 mg.h ~ via lumen 5. Each solution was infused intermittently in 15 min intervals at 1.5 ml.min ~. The infusion of AA produced significant increases in both bicarbonate and pancreatic enzyme output and in all 4 hormones in plasma. The trypsin infusion significantly inhibited both bicarbonate (p <0.01) and chymotrypsin output (p<0.05) which coincided with signiicant suppression of plasma secretin and CCK releases. Plasma PP remained unchanged (data not shown) and increased releas• of NT was not significanl; (Table). JEJUNAL INFUSION AA + NaHC0~ AA +TRYPSIN Bicarbonate output (mmol.h ~) 7.8 ± 1.5 3.1 + 0.7 ° ° Chymotrypsin output (KU.h ~) 9.5 ± 1.4 5.6 ± 0 . 7 ° Secretin (pmol.h ~) 445 ± 75 256 ± 34* CCK (pmol.h ~) 570 ± 44 435 ± 4 2 * Neurotensin (pmol.h ~) 303 ± 42 258 ± 29 * : p < 0 . 0 5 , * * p < 0 . 0 1 (compared with AA + NaHCO3) These observations suggest that pancreatic proteases control intestinally stimulated pancreatic bicarbonate and enzyme secretion via suppression of release of CCK as well as secretin.
ROLES OF GUT HORMONES IN NEGATIVE FEEDBACK REGULATION OF PANCREATIC EXOCRINE SECRETION IN HUMANS. Jin HO, Song CW, Chang TM, Chey WY. The Genesee Hospital, University of Rochester, Rochester, NY 14607, U.S.A. Negative feedback regulation of pancreatic secretion in humans has been shown to be mediated by cholecystokinin (CCK), and there are no studies investigating the effects of other hormones. We studied possible roles of secretin, CCK, neurotensin (NT) and pancreatic polypeptide (PP) on the feedback regulation in 10 healthy fasting humans. A five lumen tube with its distal open tip was positioned in proximal jejunum (PJ) under fluoroscopy, so that gastric juice (via lumen 1) and duodenal contents (via lumen 3) were collected separately by aspiration in 15 min samples while PEG solution was infused into proximal duodenum via lumen 2. An acidified (pH 2.0) 4.25% amino acid meal (AA) mixed with phenol red was infused into PJ via lumen 4 and testing solutions, either 0.1 N NaHCO3 or trypsin 600 mg.h ~ via lumen 5. Each solution was infused intermittently in 15 min intervals at 1.5 ml.min ~. The infusion of AA produced significant increases in both bicarbonate and pancreatic enzyme output and in all 4 hormones in plasma. The trypsin infusion significantly inhibited both bicarbonate (p <0.01) and chymotrypsin output (p<0.05) which coincided with signiicant suppression of plasma secretin and CCK releases. Plasma PP remained unchanged (data not shown) and increased releas• of NT was not significanl; (Table). JEJUNAL INFUSION AA + NaHC0~ AA +TRYPSIN Bicarbonate output (mmol.h ~) 7.8 ± 1.5 3.1 + 0.7 ° ° Chymotrypsin output (KU.h ~) 9.5 ± 1.4 5.6 ± 0 . 7 ° Secretin (pmol.h ~) 445 ± 75 256 ± 34* CCK (pmol.h ~) 570 ± 44 435 ± 4 2 * Neurotensin (pmol.h ~) 303 ± 42 258 ± 29 * : p < 0 . 0 5 , * * p < 0 . 0 1 (compared with AA + NaHCO3) These observations suggest that pancreatic proteases control intestinally stimulated pancreatic bicarbonate and enzyme secretion via suppression of release of CCK as well as secretin.