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NMR based metabolomic study of antifatigue effect of Astragali Radix
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Abstracts / European Journal of Integrative Medicine 6 (2014) 686–745
from the vein of the eye every two hours from 8:00 am to 8:00 pm in successive 3 days. All rats had free access to food in the first day, but were fasted in the next two days. The effect of fasting on endogenous contents could be evaluated by comparing the data from the first day and the second day, and the circadian rhythmicity could also be assessed through analyzing the data from the second day and the third day. Results: The content of endogenous cholic acid and hyodeoxycholic acid displayed significant individual differences, simultaneously, the content also exhibited a big fluctuation at different time points throughout the day and at the same point on two different days, the endogenous level was not consistent. Moreover, fasting could not reduce the level of endogenous. Conclusions: Neither fasting nor reducing endogenous contents could be applied to the pharmacokinetic study of cholic acid and hyodeoxycholic acid. Acknowledgements: This work was supported by the Foundation of Independent Topics at Beijing University of Chinese Medicine (Grant No. 2013-JYBZZ-XS-061). http://dx.doi.org/10.1016/j.eujim.2014.09.118 A study on establishing the body of evidence on postmarketing Chinese medicine safety Yanming Xie, Xing Liao ∗ , Peng-fei Lu Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, China E-mail address: [email protected] (X. Liao). At present, medical evidence grading and classification are mainly applied to effectiveness studies of drugs; the systems for evidence grading have a number of limitations when they are applied in the bio-medical field; in addition, there are also many problems arising when they are introduced into the research field of Chinese medicines (CMs). There are very few studies reporting the evidence grading and classification of postmarketing drug safety at home and abroad, so, systematical collection and evaluation of evidence of postmarketing drug safety are of important significance. Drawing on the successful experience in evidence grading and classification of drug effectiveness in evidence-based medicine, this study explores the establishment of standards for evaluation, classification and grading of safety evidence of postmarketing CMs, and the formation of the basic framework of safety evidence evaluation, and comprehensively analyzes the different sources of evidence from the perspectives of point, line and surface, in order to establish the evidence body for safety evaluation of postmarketing CMs, and provide methodology and technical support for future studies on developing the system of evidence for this field. Acknowledgement: Supported by the Research on Key Techniques of Reevaluation of Postmarketing Chinese Medicines, the Ministry of Science and Technology (No. 2009ZX09502-030), the Seventh-Science Foundation of China Academy Sciences (No. ZZ070817), and the National
Natural Science Foundation of China (General program, No. 81202776). http://dx.doi.org/10.1016/j.eujim.2014.09.119 NMR based metabolomic study of antifatigue effect of Astragali Radix Zhen-Yu Li 1 , Pan He 1,2 , Hai-Feng Sun 1 , Xue-Mei Qin 1,∗ , Guan-Hua Du 1,3 1 Modern
Research Center for Traditional Chinese Medicine of Shanxi University, No. 92, Wucheng Road, Taiyuan 030006, Shanxi, People’s Republic of China 2 College of Chemistry and Chemical Engineering of Shanxi University, No. 92, Wucheng Road, Taiyuan 030006, Shanxi, People’s Republic of China 3 Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, People’s Republic of China E-mail address: [email protected] (X.-M. Qin). Introduction: Astragali Radix (AR, Huangqi), a well-known traditional Chinese medicinal plant, has been used for centuries as a reinforcing vital energy herb in China. Previous studies revealed that AR shows obvious antifatigue effect, but its mechanism is poorly understood. In the present work, a NMR based metabolomic approach was applied to study the antifatigue effect of the AR. Methods: AR was extracted in water by refluxing and then lyophilized. 40 SD rats were randomly divided into four groups: control group (NS), AR high dose group (AH, 6 g/kg), AR middle dose group (AM, 3 g/kg) and AR low dose group (AL, 1 g/kg). The drugs were orally administered once per day for 21 days. The exhaustive swimming test was performed on the 21st day. Blood and urine were analyzed by NMR and multivariate data analysis. Results: The AM group showed longer swimming time (p < 0.05) than the NS group. The OPLS-DA score plots also showed good separations between NS and AM groups. And the corresponding S-plots indicated that the rats treated by Astragali Radix extracts showed higher levels of glucose, creatine, glycine, citrate, malate, and lower levels of lactate, O-acetylated glycoproteins, -OH-butyrate. Conclusions: The optimal antifatigue effect of AR was observed at a dose of 3 g/kg body weight. The alteration of potential biomarkers in serum and urine showed the antifatigue effect of AR could be exerted by regulating pathways of glycometabolism, lipid metabolism, energy metabolism and changes of other metabolites. http://dx.doi.org/10.1016/j.eujim.2014.09.120
Abstracts / European Journal of Integrative Medicine 6 (2014) 686–745
from the vein of the eye every two hours from 8:00 am to 8:00 pm in successive 3 days. All rats had free access to food in the first day, but were fasted in the next two days. The effect of fasting on endogenous contents could be evaluated by comparing the data from the first day and the second day, and the circadian rhythmicity could also be assessed through analyzing the data from the second day and the third day. Results: The content of endogenous cholic acid and hyodeoxycholic acid displayed significant individual differences, simultaneously, the content also exhibited a big fluctuation at different time points throughout the day and at the same point on two different days, the endogenous level was not consistent. Moreover, fasting could not reduce the level of endogenous. Conclusions: Neither fasting nor reducing endogenous contents could be applied to the pharmacokinetic study of cholic acid and hyodeoxycholic acid. Acknowledgements: This work was supported by the Foundation of Independent Topics at Beijing University of Chinese Medicine (Grant No. 2013-JYBZZ-XS-061). http://dx.doi.org/10.1016/j.eujim.2014.09.118 A study on establishing the body of evidence on postmarketing Chinese medicine safety Yanming Xie, Xing Liao ∗ , Peng-fei Lu Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, China E-mail address: [email protected] (X. Liao). At present, medical evidence grading and classification are mainly applied to effectiveness studies of drugs; the systems for evidence grading have a number of limitations when they are applied in the bio-medical field; in addition, there are also many problems arising when they are introduced into the research field of Chinese medicines (CMs). There are very few studies reporting the evidence grading and classification of postmarketing drug safety at home and abroad, so, systematical collection and evaluation of evidence of postmarketing drug safety are of important significance. Drawing on the successful experience in evidence grading and classification of drug effectiveness in evidence-based medicine, this study explores the establishment of standards for evaluation, classification and grading of safety evidence of postmarketing CMs, and the formation of the basic framework of safety evidence evaluation, and comprehensively analyzes the different sources of evidence from the perspectives of point, line and surface, in order to establish the evidence body for safety evaluation of postmarketing CMs, and provide methodology and technical support for future studies on developing the system of evidence for this field. Acknowledgement: Supported by the Research on Key Techniques of Reevaluation of Postmarketing Chinese Medicines, the Ministry of Science and Technology (No. 2009ZX09502-030), the Seventh-Science Foundation of China Academy Sciences (No. ZZ070817), and the National
Natural Science Foundation of China (General program, No. 81202776). http://dx.doi.org/10.1016/j.eujim.2014.09.119 NMR based metabolomic study of antifatigue effect of Astragali Radix Zhen-Yu Li 1 , Pan He 1,2 , Hai-Feng Sun 1 , Xue-Mei Qin 1,∗ , Guan-Hua Du 1,3 1 Modern
Research Center for Traditional Chinese Medicine of Shanxi University, No. 92, Wucheng Road, Taiyuan 030006, Shanxi, People’s Republic of China 2 College of Chemistry and Chemical Engineering of Shanxi University, No. 92, Wucheng Road, Taiyuan 030006, Shanxi, People’s Republic of China 3 Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, People’s Republic of China E-mail address: [email protected] (X.-M. Qin). Introduction: Astragali Radix (AR, Huangqi), a well-known traditional Chinese medicinal plant, has been used for centuries as a reinforcing vital energy herb in China. Previous studies revealed that AR shows obvious antifatigue effect, but its mechanism is poorly understood. In the present work, a NMR based metabolomic approach was applied to study the antifatigue effect of the AR. Methods: AR was extracted in water by refluxing and then lyophilized. 40 SD rats were randomly divided into four groups: control group (NS), AR high dose group (AH, 6 g/kg), AR middle dose group (AM, 3 g/kg) and AR low dose group (AL, 1 g/kg). The drugs were orally administered once per day for 21 days. The exhaustive swimming test was performed on the 21st day. Blood and urine were analyzed by NMR and multivariate data analysis. Results: The AM group showed longer swimming time (p < 0.05) than the NS group. The OPLS-DA score plots also showed good separations between NS and AM groups. And the corresponding S-plots indicated that the rats treated by Astragali Radix extracts showed higher levels of glucose, creatine, glycine, citrate, malate, and lower levels of lactate, O-acetylated glycoproteins, -OH-butyrate. Conclusions: The optimal antifatigue effect of AR was observed at a dose of 3 g/kg body weight. The alteration of potential biomarkers in serum and urine showed the antifatigue effect of AR could be exerted by regulating pathways of glycometabolism, lipid metabolism, energy metabolism and changes of other metabolites. http://dx.doi.org/10.1016/j.eujim.2014.09.120