- Email: [email protected]
No association between helicobacter pylori and chronic urticaria
Free communication
FC28.
FC28.
IFC28
MiscellaneoudUrticaria Onychomycosis and management
in children:
Belgium
Effect of cylcosporine and retinoic acid on the production of IL-8 by THP-1 cell line
D. SvecivB’, T. Kawashima*, A. Ohkawara*. ‘Dep. of Dermatology, School of Medicine, Commenius University, Bratislava, Slovak Republic; 2Dep. of Dermatology, School Medicine,
Hokkaido
University,
between Helicobacter 3 No association pylori and chronic urticaria
Amsterdam,
Onychomycosis in children is often accompanied by tinea pedis and a family history of onychomycosis. The prevalence of onychomycosis in children is substantially lower than that of adults; therefore, it is important to confirm the clinical diagnosis. The most common presentation of onychomycosis is toenail disease, and this is of the distal and lateral subungual type. The organism most commdnly isolated in North America is Trichophyton rubrurn. Oral antifungal therapy is required, especially when the onychomycosis is of moderate-to-severe intensity, with nail matrix involvement. The new oral antifungal agents itraconazole, terbinafine, and fluconazole are being increasingly used for the treatment of onychomycosis. Review of the literature suggests that these agents are effective and afe in managing onychomycosis in children. The short duration of therapy required with these drugs should help improve compliance. For some children the availability of a liquid formulation may be beneficial. The data suggest that the new oral antifungal agents have a role in the treatment of onychomycosis in children. Further experience will help us better position these drugs when evaluating the management of children with onychomycosis. FC28-2
Sapporo,
Sl79
B. Kijycil, M.M.A. Koze1,M.M.H.M. Meinardi, J.D. Bos. Dtipartment of Dermatology, Academisch Medisch Centrum.
Prevalence
A.K. Gupta’, P. Chang*, J.Q. Del Rosso3, P De Donckefi. t University of Toronto, Canada; 2Hospital Angeles, Guatemala; ‘University of Nevada, USA; 4Janssen Research Foundation,
Miscellaneous/fJrticaria
Objective: To investigate the relationship between chronic urticaria and/or angio-oedema and H. pylori infection. Methods: Patients with chronic urticaria and/or angio-edema were investigated for H. pylori specific IgG-antibodies by reverse-flow immunochromatografy. Three groups were formed: a group of H. pylon’ positive patients that received eradication therapy, a group of H. pylon’ negative patients that received eradication therapy and a group of H. pylori negative patients that did not received eradication therapy. All patients were evaluated after I, 3 and 9 weeks after the eradication of H. pylori. A 4-scaled questionairre was used to detect differences between the complaints. The outcome after 9 weeks was considered to be definitive. Results: Thirty-one patients were selected. The mean follow-up was 11 weeks (range 9-16 weeks). None of the patients were treated in the past with triple therapy to eradicate H. pylon’. In 15 patients, H. pylon’ specific IgCi-antibodies were detected. In I6 patients no H. pylori specific IgG was detected. Of these 16, 7 patients were treated with eradication therapy. We found that the percentages of partial remission, complete remission, aggrivation and stable disease were equal among the three groups. Conclusion: In contrast to the current literature, our study failed to show a beneficial effect of H. pylori eradication treatment on the outcome of chronic urticaria. Therefore, a direct relation between chronic u&aria and/or angio-oedema and H. pylori infection could not be confirmed. I FC28 4
of
Japan
IL-8 is a member of C-X-C chemokines and plays a very important role in inflammation. Its level is about 150-times higher in the psoriasis affected skin. It suggests that IL-8 may play an important role in the pathogenesis of psoriasis. The precise mechanism of cyclosporine (CsA) and retinoic acid (RA) effects are not known. The aim of experiment was to find out CsA and RA effects on production of IL-8 by THP-1 cell line. THP-1 cell line was cultavted in completed RPMI-I640 medium and stimulated with LPS. The level of IL-8 was evaluated by human ELISA kits. Student’s test was used for statistical analysis. It was found out that CsA inhibits IL-S production by stimulated THP-1 cell line in dose dependence course. RA promotes IL-8 production also in dose dependence course.
The Netherlands
Psoriasis: Several types of abnormalities in the duodenal mucosa
G. Michaglsson, W. Kraaz, E. Hagforsen, I. Pihl-Lundin. Dermatology & Venereology, Uppsala, Sweden
University
Hospital,
Dep.
751 85
Patients with psoriasis (n = 37) and with irritable bowel syndrome (IBS, n = 22) underwent gastroscopy with duodenal biopsies. Serum IgA/IgG antibodies to gliadin were determined. Intraepithelial mononuclear cells were scored O-3, CD3+ and y/S+ T lymphocytes in tbe epithelium were counted as were tryptase+ mast cells and EG2+ eosinophils in the stroma. An increased intraepithelial mononuclear celI infiltration and increased number of CD3 and y/S+ T lymphocytes was observed in 12 patients with moderate and severe psoriasis; 9 of them with IgA antibodies to gliadin and 3 with IgG antibodies to gliadin. After 3 months on gluten-free diet a significant improvement of the PAS1 score was observed in patients with antibodies to gliadin. Most psoriasis patients (33/37) had a pronounced increase in number of mast cells in the stroma -131 f 58 vs 28 f 18/mm2 for the IBS group. The number of EG2+ eosinopbils was also increased and correlated to that of the mast cells (p = 0.0001). The increase in mast cells and eosinophils was not associated with raised levels of antibodies to gliadin or increased infiltration of lymphocytes in the epithelium.
FC28.
FC28.
IFC28
MiscellaneoudUrticaria Onychomycosis and management
in children:
Belgium
Effect of cylcosporine and retinoic acid on the production of IL-8 by THP-1 cell line
D. SvecivB’, T. Kawashima*, A. Ohkawara*. ‘Dep. of Dermatology, School of Medicine, Commenius University, Bratislava, Slovak Republic; 2Dep. of Dermatology, School Medicine,
Hokkaido
University,
between Helicobacter 3 No association pylori and chronic urticaria
Amsterdam,
Onychomycosis in children is often accompanied by tinea pedis and a family history of onychomycosis. The prevalence of onychomycosis in children is substantially lower than that of adults; therefore, it is important to confirm the clinical diagnosis. The most common presentation of onychomycosis is toenail disease, and this is of the distal and lateral subungual type. The organism most commdnly isolated in North America is Trichophyton rubrurn. Oral antifungal therapy is required, especially when the onychomycosis is of moderate-to-severe intensity, with nail matrix involvement. The new oral antifungal agents itraconazole, terbinafine, and fluconazole are being increasingly used for the treatment of onychomycosis. Review of the literature suggests that these agents are effective and afe in managing onychomycosis in children. The short duration of therapy required with these drugs should help improve compliance. For some children the availability of a liquid formulation may be beneficial. The data suggest that the new oral antifungal agents have a role in the treatment of onychomycosis in children. Further experience will help us better position these drugs when evaluating the management of children with onychomycosis. FC28-2
Sapporo,
Sl79
B. Kijycil, M.M.A. Koze1,M.M.H.M. Meinardi, J.D. Bos. Dtipartment of Dermatology, Academisch Medisch Centrum.
Prevalence
A.K. Gupta’, P. Chang*, J.Q. Del Rosso3, P De Donckefi. t University of Toronto, Canada; 2Hospital Angeles, Guatemala; ‘University of Nevada, USA; 4Janssen Research Foundation,
Miscellaneous/fJrticaria
Objective: To investigate the relationship between chronic urticaria and/or angio-oedema and H. pylori infection. Methods: Patients with chronic urticaria and/or angio-edema were investigated for H. pylori specific IgG-antibodies by reverse-flow immunochromatografy. Three groups were formed: a group of H. pylon’ positive patients that received eradication therapy, a group of H. pylon’ negative patients that received eradication therapy and a group of H. pylori negative patients that did not received eradication therapy. All patients were evaluated after I, 3 and 9 weeks after the eradication of H. pylori. A 4-scaled questionairre was used to detect differences between the complaints. The outcome after 9 weeks was considered to be definitive. Results: Thirty-one patients were selected. The mean follow-up was 11 weeks (range 9-16 weeks). None of the patients were treated in the past with triple therapy to eradicate H. pylon’. In 15 patients, H. pylon’ specific IgCi-antibodies were detected. In I6 patients no H. pylori specific IgG was detected. Of these 16, 7 patients were treated with eradication therapy. We found that the percentages of partial remission, complete remission, aggrivation and stable disease were equal among the three groups. Conclusion: In contrast to the current literature, our study failed to show a beneficial effect of H. pylori eradication treatment on the outcome of chronic urticaria. Therefore, a direct relation between chronic u&aria and/or angio-oedema and H. pylori infection could not be confirmed. I FC28 4
of
Japan
IL-8 is a member of C-X-C chemokines and plays a very important role in inflammation. Its level is about 150-times higher in the psoriasis affected skin. It suggests that IL-8 may play an important role in the pathogenesis of psoriasis. The precise mechanism of cyclosporine (CsA) and retinoic acid (RA) effects are not known. The aim of experiment was to find out CsA and RA effects on production of IL-8 by THP-1 cell line. THP-1 cell line was cultavted in completed RPMI-I640 medium and stimulated with LPS. The level of IL-8 was evaluated by human ELISA kits. Student’s test was used for statistical analysis. It was found out that CsA inhibits IL-S production by stimulated THP-1 cell line in dose dependence course. RA promotes IL-8 production also in dose dependence course.
The Netherlands
Psoriasis: Several types of abnormalities in the duodenal mucosa
G. Michaglsson, W. Kraaz, E. Hagforsen, I. Pihl-Lundin. Dermatology & Venereology, Uppsala, Sweden
University
Hospital,
Dep.
751 85
Patients with psoriasis (n = 37) and with irritable bowel syndrome (IBS, n = 22) underwent gastroscopy with duodenal biopsies. Serum IgA/IgG antibodies to gliadin were determined. Intraepithelial mononuclear cells were scored O-3, CD3+ and y/S+ T lymphocytes in tbe epithelium were counted as were tryptase+ mast cells and EG2+ eosinophils in the stroma. An increased intraepithelial mononuclear celI infiltration and increased number of CD3 and y/S+ T lymphocytes was observed in 12 patients with moderate and severe psoriasis; 9 of them with IgA antibodies to gliadin and 3 with IgG antibodies to gliadin. After 3 months on gluten-free diet a significant improvement of the PAS1 score was observed in patients with antibodies to gliadin. Most psoriasis patients (33/37) had a pronounced increase in number of mast cells in the stroma -131 f 58 vs 28 f 18/mm2 for the IBS group. The number of EG2+ eosinopbils was also increased and correlated to that of the mast cells (p = 0.0001). The increase in mast cells and eosinophils was not associated with raised levels of antibodies to gliadin or increased infiltration of lymphocytes in the epithelium.