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No evidence of lyme disease in Australia yet
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PATHOLOGY 2015 ABSTRACT SUPPLEMENT
This case highlights an unusual presentation of E. histolytica infection, and serves as a reminder that endemic transmission of this parasite is not only possible, but becoming increasingly recognised.
Pathology (2015), 47(S1)
of carbapenem resistance in Aeromonas spp. is essential to guide appropriate antimicrobial therapy.
NO EVIDENCE OF LYME DISEASE IN AUSTRALIA YET GENOTYPIC AND PHENOTYPIC IDENTIFICATION OF AEROMONAS SPECIES AND CARBAPENEM RESISTANCE IN QUEENSLAND, AUSTRALIA H. A. Sinclair, C. Heney, H. E. Sidjabat, N. M. George, H. Bergh, S. N. Anuj, G. R. Nimmo and D. L. Paterson Pathology Queensland and University of Queensland Centre for Clinical Research, Qld, Australia Aims: This study defines the population of Aeromonas spp. in Queensland and carbapenem resistance due to carbapenemase production, CphA. Phenotypic tests were correlated with the presence of the cphA gene. Methods: Isolates from blood (22), wound (46), sterile sites (11), stool (18), eye (2) and sputum (1) were characterised by rpoB and gyrB sequencing. Disc diffusion and meropenem E-test MIC were determined. Carbapenemase production was assessed by Carba NP test and cphA by PCR. Results: Isolates identified as A. dhakensis (39), A. veronii (21), A. hydrophila (20), A. caviae (14), A. jandaei (4), A. bestiarum (1), and A. sanarellii (1). Disc diffusion and E-test failed to demonstrate carbapenem resistance. Carba NP test performed with 97.4% sensitivity and 95.7% specificity. The cphA gene was detected in A. veronii (21; 100%), A. hydrophila (18; 90%), A. dhakensis (34; 87.2%), A. jandaei (2; 50%), A. bestiarum (1; 100%) and not A. caviae. Discussion: Phylogenetic analysis revealed A. dhakensis caused majority of infection, an unrecognised pathogen in this region. Carbapenem resistance due to cphA was prevalent. The Carba NP was a reliable phenotypic test. Adequate speciation and detection
John Stenos1, Gemma Vincent1, Mythili Tadepalli1, Chelsea Nguyen1, Hazizul Hussain1, Aminul Islam1,2 and Stephen Graves1,2 1Australian Rickettsial Reference Laboratory, Barwon Health, Geelong Vic, and 2Division of Microbiology, Pathology North Hunter, NSW Health Pathology, Newcastle, NSW, Australia Aims: To develop a screening protocol for detecting Lyme disease in Australia. Methods: A protocol for the serological screening of sera was established whereby the initial screen was performed by enzyme immunosorbent and immunofluorescence assay and if both assays were positive a confirmatory testing was performed by Western blot. Culture and qPCR assays were used to screen human blood and tick lysates. Results: All known Lyme disease positive sera obtained from Europe and the USA have been positive in all three assays confirming the ability of these assays to detect genuine cases of this disease. No Australian serum (in a non-traveller) has tested positive in all three assays suggesting that no person has been infected with a Borrelia spp. to date in Australia. Similarly, the testing of patient bloods by qPCR and culture have all been negative. Screening of Australian ticks for Borrelia spp. has yielded negative results, supporting the view that these ticks do not carry nor transmit Lyme disease. Discussion: While it is impossible to prove the absence of a Lyme disease in Australia and while keeping an open mind on this possibility, this data suggests that Lyme disease does not occur in Australia.
Copyright © Royal College of pathologists of Australasia. Unauthorized reproduction of this article is prohibited.
PATHOLOGY 2015 ABSTRACT SUPPLEMENT
This case highlights an unusual presentation of E. histolytica infection, and serves as a reminder that endemic transmission of this parasite is not only possible, but becoming increasingly recognised.
Pathology (2015), 47(S1)
of carbapenem resistance in Aeromonas spp. is essential to guide appropriate antimicrobial therapy.
NO EVIDENCE OF LYME DISEASE IN AUSTRALIA YET GENOTYPIC AND PHENOTYPIC IDENTIFICATION OF AEROMONAS SPECIES AND CARBAPENEM RESISTANCE IN QUEENSLAND, AUSTRALIA H. A. Sinclair, C. Heney, H. E. Sidjabat, N. M. George, H. Bergh, S. N. Anuj, G. R. Nimmo and D. L. Paterson Pathology Queensland and University of Queensland Centre for Clinical Research, Qld, Australia Aims: This study defines the population of Aeromonas spp. in Queensland and carbapenem resistance due to carbapenemase production, CphA. Phenotypic tests were correlated with the presence of the cphA gene. Methods: Isolates from blood (22), wound (46), sterile sites (11), stool (18), eye (2) and sputum (1) were characterised by rpoB and gyrB sequencing. Disc diffusion and meropenem E-test MIC were determined. Carbapenemase production was assessed by Carba NP test and cphA by PCR. Results: Isolates identified as A. dhakensis (39), A. veronii (21), A. hydrophila (20), A. caviae (14), A. jandaei (4), A. bestiarum (1), and A. sanarellii (1). Disc diffusion and E-test failed to demonstrate carbapenem resistance. Carba NP test performed with 97.4% sensitivity and 95.7% specificity. The cphA gene was detected in A. veronii (21; 100%), A. hydrophila (18; 90%), A. dhakensis (34; 87.2%), A. jandaei (2; 50%), A. bestiarum (1; 100%) and not A. caviae. Discussion: Phylogenetic analysis revealed A. dhakensis caused majority of infection, an unrecognised pathogen in this region. Carbapenem resistance due to cphA was prevalent. The Carba NP was a reliable phenotypic test. Adequate speciation and detection
John Stenos1, Gemma Vincent1, Mythili Tadepalli1, Chelsea Nguyen1, Hazizul Hussain1, Aminul Islam1,2 and Stephen Graves1,2 1Australian Rickettsial Reference Laboratory, Barwon Health, Geelong Vic, and 2Division of Microbiology, Pathology North Hunter, NSW Health Pathology, Newcastle, NSW, Australia Aims: To develop a screening protocol for detecting Lyme disease in Australia. Methods: A protocol for the serological screening of sera was established whereby the initial screen was performed by enzyme immunosorbent and immunofluorescence assay and if both assays were positive a confirmatory testing was performed by Western blot. Culture and qPCR assays were used to screen human blood and tick lysates. Results: All known Lyme disease positive sera obtained from Europe and the USA have been positive in all three assays confirming the ability of these assays to detect genuine cases of this disease. No Australian serum (in a non-traveller) has tested positive in all three assays suggesting that no person has been infected with a Borrelia spp. to date in Australia. Similarly, the testing of patient bloods by qPCR and culture have all been negative. Screening of Australian ticks for Borrelia spp. has yielded negative results, supporting the view that these ticks do not carry nor transmit Lyme disease. Discussion: While it is impossible to prove the absence of a Lyme disease in Australia and while keeping an open mind on this possibility, this data suggests that Lyme disease does not occur in Australia.
Copyright © Royal College of pathologists of Australasia. Unauthorized reproduction of this article is prohibited.