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Noradrenergic modulation of long-term potentiation in kitten visual cortex
S146
MAGNETIC RESONANCE IMAGING*OF JAPANESE MONKEY BRAIN: COMPARED WITH X-RAY PHOTOGRAPHY AND HISTOkOGY SHINJI KAJIl --* KEIJI MATSUDA KENJI KAWANO, HIDEHIKO KOMATSU, ---~SHIGERU YAMANE, #TAKASHI YOSIZAWA and -#TADA~ NOSE Neuroscience Sect. E.T.L. Tsukubashl, Ibaraki -305 JAPAN. #Neurosurqery, ~Medlcal Sch. --. Tsukuba Univ. Tsukubashi, -Ibaraki 305, JAPAN -The localization of a small target area in the brain such as a lesion, a" electrode tip for single unit recording, or a mlcroneedle tip for drug appllcatlo" is usually estimated by using stereotaxic atlases, asslsted by X-ray photography or electrophyslologlcal mapping, and determined finally by hlstologlcal reconstruction. Magnetic Resonance Imaging (MRI) can visualize cross sections in any plane of three dimensional structures of the brain noninvaslvely . It can discriminate sulci and gyri of the cortex, as well as gray and white matter. We compared images of MRI, X-ray, and histology from a monkey bran. A Japanese monkey (Mcaca fuscata, 8, 11 kg) was anesthetlsed, -fixed in a newly developed magnetic-free stereotaxic apparatus, and mounted 1" the MRI scanner unit (BRUKER, BIOSPEC 24/40, 2.4 Della). Some para-saggltal images (2.5 mm thick) and para-frontal (2.5 mm thick, every 5 mm distance) images were obtalned. The outline of the bone on the MRI image was compared with that on the X-ray photograph take" by a" X-ray u~strument(Toshlba, TR-EOA). The two images fitted very well. The anunal was sacrificed and the braI" was flxed by a" infuslon method. The brain was sliced in 100 pm with a freezlng method and stained with Cresyl violet. The histological preparations were shrunk some 10% during the process, which was revealed by comparison of MRI, X-ray, and hlstologlcal images. I" co"clus1o", MRI unages are reliable enough to determxxe a small target in deep structures of the brain, and thex superunposed images on X-rays will assist 1" ldentifylng the location of electrode or needle tips.
46. Visual system
III. Development
NORADRENERGIC MODULATION OF Department YUKIO KOMATSU,
LONG-TERM
POTENTIATION
IN
KITTEN
VISUAL
CORTEX.
of
Physiology, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamisyoku, Kyoto 602, Japan. Involvement of noradrenaline in long-term potentiation in kitten (LTP) visual cortex was examined in slices prepared from kittens aged 25-40 days. LTP was assessed by current source density analysis of field potentials evoked by low frequency (0.1 Hz) test stimulation of white matter before and after conditioning stimulation (2 Hz, 15 minutes) of white matter. LTP of late currents in layers II-III which represent cortico-cortical and V, synaptic transmission, was facilitated by bath application of noradrenaline B-agonist (30 PM) or a isoproterenol (2 PM) and was partly depressed by a Eantagonist timolol (20 FM). However, these not affect LTP of early currents in layers IV and VI, agents did which mostly represent geniculo-cortical synaptic transmission. The residual LTP the presence of timolol seems to be secondary to the LTP of late currents under of currents, timolol totally prevented LTP induced by early since 6 PM axon collaterals of conditioning stimulation of layer I, which directly activates pathways to These presynaptic cells of cortico-cortical layers II-III and V. results indicate that noradrenaline facilitates the LTP of cortico-cortical not affect the LTP of synaptic transmission through %-receptors but it does geniculo-cortical synaptic transmission.
DEVELOPMENTAL CHANGES OF MOLECULAR EXPRESSION IN KITTEN VISUAL CORTEX. KAZUYUKI IMAMURAl, KENSAKU MORIl, SHOGO OKAl, YASUYOSHI WATANABEl, TOMOAKI SHIRA02, 6-2-4 KUNIHIKO OBATAL, 1Department of Neuroscience, Osaka Bioscience InStitU'Ce, Neurochemistry, National Osaka 565, Japan, 2Laboratory of Suita-shi, Furuedai, Institute for Physiological Science, Myodaiji, Okazaki 444, Japan. studied was cortex (area kitten visual 17) Development of and a telencephalin antibody against immunohistochemically using a polyclonal Telencephalin is a membrane-glycoprotein antibody against drebrin. monoclonal Drebrin is a developmentallyin telencephalic regions. exclusively expressed regions, particularly at synaptic brain protein localized in dendrites, regulated antibody Polyclonal and changes its expression according to developmental stages. telencephalin bound to layer IV as well as other layers of area 17 during against period (3-13 wk old of age). Outside the sensitive period, the the sensitive the visual Immunostaining of layer IV was selectively suppressed. staining of the cortex with monoclonal antibody against drebrin was observed preferentially in Changes early postnatal period and decreased by the end of the sensitive period. the sensitive in staining patterns with these antibodies were closely related to level. Present results plasticity is sustained at a high during which period that the expression of these proteins is developmentally-regulated in an suggest early postnatal period and may play a role in regulating plasticity during the sensitive period.
MAGNETIC RESONANCE IMAGING*OF JAPANESE MONKEY BRAIN: COMPARED WITH X-RAY PHOTOGRAPHY AND HISTOkOGY SHINJI KAJIl --* KEIJI MATSUDA KENJI KAWANO, HIDEHIKO KOMATSU, ---~SHIGERU YAMANE, #TAKASHI YOSIZAWA and -#TADA~ NOSE Neuroscience Sect. E.T.L. Tsukubashl, Ibaraki -305 JAPAN. #Neurosurqery, ~Medlcal Sch. --. Tsukuba Univ. Tsukubashi, -Ibaraki 305, JAPAN -The localization of a small target area in the brain such as a lesion, a" electrode tip for single unit recording, or a mlcroneedle tip for drug appllcatlo" is usually estimated by using stereotaxic atlases, asslsted by X-ray photography or electrophyslologlcal mapping, and determined finally by hlstologlcal reconstruction. Magnetic Resonance Imaging (MRI) can visualize cross sections in any plane of three dimensional structures of the brain noninvaslvely . It can discriminate sulci and gyri of the cortex, as well as gray and white matter. We compared images of MRI, X-ray, and histology from a monkey bran. A Japanese monkey (Mcaca fuscata, 8, 11 kg) was anesthetlsed, -fixed in a newly developed magnetic-free stereotaxic apparatus, and mounted 1" the MRI scanner unit (BRUKER, BIOSPEC 24/40, 2.4 Della). Some para-saggltal images (2.5 mm thick) and para-frontal (2.5 mm thick, every 5 mm distance) images were obtalned. The outline of the bone on the MRI image was compared with that on the X-ray photograph take" by a" X-ray u~strument(Toshlba, TR-EOA). The two images fitted very well. The anunal was sacrificed and the braI" was flxed by a" infuslon method. The brain was sliced in 100 pm with a freezlng method and stained with Cresyl violet. The histological preparations were shrunk some 10% during the process, which was revealed by comparison of MRI, X-ray, and hlstologlcal images. I" co"clus1o", MRI unages are reliable enough to determxxe a small target in deep structures of the brain, and thex superunposed images on X-rays will assist 1" ldentifylng the location of electrode or needle tips.
46. Visual system
III. Development
NORADRENERGIC MODULATION OF Department YUKIO KOMATSU,
LONG-TERM
POTENTIATION
IN
KITTEN
VISUAL
CORTEX.
of
Physiology, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamisyoku, Kyoto 602, Japan. Involvement of noradrenaline in long-term potentiation in kitten (LTP) visual cortex was examined in slices prepared from kittens aged 25-40 days. LTP was assessed by current source density analysis of field potentials evoked by low frequency (0.1 Hz) test stimulation of white matter before and after conditioning stimulation (2 Hz, 15 minutes) of white matter. LTP of late currents in layers II-III which represent cortico-cortical and V, synaptic transmission, was facilitated by bath application of noradrenaline B-agonist (30 PM) or a isoproterenol (2 PM) and was partly depressed by a Eantagonist timolol (20 FM). However, these not affect LTP of early currents in layers IV and VI, agents did which mostly represent geniculo-cortical synaptic transmission. The residual LTP the presence of timolol seems to be secondary to the LTP of late currents under of currents, timolol totally prevented LTP induced by early since 6 PM axon collaterals of conditioning stimulation of layer I, which directly activates pathways to These presynaptic cells of cortico-cortical layers II-III and V. results indicate that noradrenaline facilitates the LTP of cortico-cortical not affect the LTP of synaptic transmission through %-receptors but it does geniculo-cortical synaptic transmission.
DEVELOPMENTAL CHANGES OF MOLECULAR EXPRESSION IN KITTEN VISUAL CORTEX. KAZUYUKI IMAMURAl, KENSAKU MORIl, SHOGO OKAl, YASUYOSHI WATANABEl, TOMOAKI SHIRA02, 6-2-4 KUNIHIKO OBATAL, 1Department of Neuroscience, Osaka Bioscience InStitU'Ce, Neurochemistry, National Osaka 565, Japan, 2Laboratory of Suita-shi, Furuedai, Institute for Physiological Science, Myodaiji, Okazaki 444, Japan. studied was cortex (area kitten visual 17) Development of and a telencephalin antibody against immunohistochemically using a polyclonal Telencephalin is a membrane-glycoprotein antibody against drebrin. monoclonal Drebrin is a developmentallyin telencephalic regions. exclusively expressed regions, particularly at synaptic brain protein localized in dendrites, regulated antibody Polyclonal and changes its expression according to developmental stages. telencephalin bound to layer IV as well as other layers of area 17 during against period (3-13 wk old of age). Outside the sensitive period, the the sensitive the visual Immunostaining of layer IV was selectively suppressed. staining of the cortex with monoclonal antibody against drebrin was observed preferentially in Changes early postnatal period and decreased by the end of the sensitive period. the sensitive in staining patterns with these antibodies were closely related to level. Present results plasticity is sustained at a high during which period that the expression of these proteins is developmentally-regulated in an suggest early postnatal period and may play a role in regulating plasticity during the sensitive period.