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O513 Combination of hysteroscopy and laparoscopy in the treatment of 167 cases of salpingian infertility
Free communication (oral) presentations / International Journal of Gynecology & Obstetrics 107S2 (2009) S93–S396
prevention of sexually transmitted diseases is a stable, harmonious relationship, with a faithful partner. A necessary condition prior to the onset of sexual intercourse is a physical and mental maturity of both partners. It is recommended that the partner who has a urinary or sexually transmitted infection should first recover and then together with the partner maintain normal sexual hygiene. For systematic prevention of sexually transmitted infections diseases in this moment it is necessary: introduce an effective sexual aducation in schools starting from primary school, develop interdisciplinary cooperation between social and medical sciences, including all experts. Stil the best and most important education comes from a healthy family as the core of our society. We want to do something for that young population for education of Human Papillomavirus so we organized High school rock concerts. In appropriate assembly rooms of some Grammar and Secondary School of Zagreb during weekend evenings (Fridays, Saturdays) educational lectures held by medical specialist, were given about sexually transmitted deseases, particularly HPV and after the lectures rock concerts were held by some checked out rock groups and others young so-called "demo" group consisting of secondary school students of Zagreb. Lectures were short (each 20–30 min) accompanied with discussion. Lecture was given in the form of Power Point presentation. By organizing concerts the interest of that population to attend would be greater. Rock concerts after the lecture in some kind of bait for that population to be present at the lecture. By offering free refreshing beverages and media support by radio listened mostly by the young as well as the musical web portal, it has been tried to make popular the whole project. In all given lectures the lecture rooms were too small to accept all audience interested in lecture. Questions made after the lecture were those usual for that age. They asked about the way of contracting HPV, medical treatment of partners and use of contraceptives and aslo about vaccina. Booklets explaining in a popular way the sexually transmitted diseases, way of catching infection and protecting methods were distributed. Mass media have excellently marked the whole project with almost everyday information. We continued with this project also in the this school year. O511 The effectiveness of an educational program for visual inspection with acetic acid (VIA) as cervical cancer screening in Ugandan health care workers: A pilot project L. Levine1 , E. Banks1 , S. Chudnoff1 , K. Taylor2 , C. Kahn1 , C. Eleanor3 . 1 Department of Obstetrics and Gynecology, Montefiore Medical Center, Albert Einstein College of Medicine, 2 Prevention International No Cervical Cancer, 3 Albert Einstein College of Medicine Objectives: To assess a brief, intense, educational intervention implementing VIA amongst diverse healthcare providers. Methods: This is a prospective analysis of an educational modality utilizing a pretest/posttest model. Subjects were physicians, midwives, nurses, and health officers serving women in Kisoro, Uganda recruited via posted announcements and personal invitation. Subjects were surveyed regarding basic demographics and pretested for knowledge in anatomy, cervical dysplasia, cervical cancer, and VIA. Subjects had interactive lectures on these topics and then observed screening by experts and performed screening in a mentored setting. Participants were retested with a validated instrument and technical skills were assessed on live patients at the conclusion of the 5 day workshop. Results: 15 healthcare workers participated in the initial screen and educational modality. The mean scores on the pretest and posttest were 60.9% and 80.3%, respectively (p < 0.001). Scores of the physicians and non-physicians were significantly different at 73.7% and 56.4%, respectively for the pretest (p < 0.01) and 75.2% and
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94.0%, p < 0.01 for the posttest. Self-assessment of comfort increased after the training session (p < 0.001). There was a correlation between the participant’s score and the number of times they had seen the procedures. Conclusions: Our program improved knowledge in cervical anatomy and cervical cancer screening using VIA, irrespective of prior training. While level of prior training did correlate with scores on the pretest and posttest, the number of years of experience did not directly correlate with their results. Importantly, the level of comfort after the course did increase in every level of training. O512 Effects of ovarian carcinoma cells on the maturation and function of human dendritic cells C. Li, H. Duan, W. Zhang To investigate the mechanisms of dendritic cells (DC) defective function in tumor that allow them to escape initial immune recognition and destruction, observed the effects of ovarian carcinoma cells on the differentiation, maturation, and function of the mononuclear cell-derived DC in vitro. Ovarian carcinoma cells were used as the conditioned cultural medium for DC culture, while the human bone marrow stromal cells (HFCL) were used as control. And transwell system was used to no intercellular contact culture. The dendritic cells were characterized by phenotypic analysis and evaluated the ability to uptake dextran and stimulate allogeneic T cells of DC. Furthermore, mmune related cytokines were detected. The results show that DC cultured in the presence of ovarian carcinoma cells expressed lower levels of CD1a, MHC-II molecules (HLA-DR) and costimulatory molecules (CD86), and expressed higher CD83 (a typical molecule of mature DC) than that in control group. Moreover, they displayed a much lower ability of phagocytizing dextran and less efficient of inducing allogeneic T-cell proliferation in a mixed lymphocyte reaction, and downregulated IL-12 secretion were also observed. In conclusion, our data suggested ovarian carcinoma cells negatively regulate the production and function of DC by release soluble factors and intercellular contact, could suppress monocyte differentiation into DCs, the most potent antigen-presenting cells (APCs). which might be one of the underlying mechanisms that plays a role in the immune escape of tumor cells. O513 Combination of hysteroscopy and laparoscopy in the treatment of 167 cases of salpingian infertility C. Li, H. Duan, Y. Liu To analyze the clinical value of using hysteroscopy combined with laparoscopy in the diagnosis and treatment of salpingian infertility. 160 cases of salpingian infertility (326 oviducts) performed hysteroscopy combined with laparoscopy, which had been diagnosed by hysterosalpinggography before operation, data was compared and analyzed. In all of the 167 cases (312 abnormal oviducts) diagnosed by hysterosalpinggography, 164 oviducts were not found abnormal (52.56%, 164/312), 4 oviducts were unobstructed but adherence with periphery (1.28%, 4/312), 48 oviducts were interstitial tubal obstruction (15.38%, 48/312), and 96 oviducts were fallopian obstruction with hydrosalpinx (30.77%, 96/312). Otherwise, 2 of 14 normal oviducts diagnosed by hysterosalpinggography were unobstructed but adherence with periphery. The consistency ratio is 49.08% (160/326). Used combination of hysteroscopy and laparoscopy as “golden rule” of diagnosis, the sensitivity of hysterosalpinggography was 98.66%, specificity was 6.82%, positive predictive value was 47.44%, negative predictive value was 85.71%. All the cases of salpingian abnormal were treated during operation, and no complication.
S240
Free communication (oral) presentations / International Journal of Gynecology & Obstetrics 107S2 (2009) S93–S396
In conclusions, hysteroscopy combined with laparoscopy could supply direct diagnosis method for salpingian abnormal, and treat at same time, which is the best way in the diagnosis and treatment of salpingian infertility. O514 The values of serum human epididymis secretory protein 4 and CA125 assay in the diagnosis of ovarian malignance H. Cui1 , D. Li2 , X. Chang3 , X. Ye3 , H. Cheng3 . 1 Gynecologic Oncology Center, Peking University People’ s Hospital, 2 Department of Gynecology and Obstetrics, Beijing Jishuitan Hospital, 3 Gynecologic Oncology Center, Peking University People’s Hospital Objective: To evaluate the value of human epididymis secretory protein 4 (HE4) and CA125 in the diagnosis of ovarian malignance. Methods: HE4 and CA125 in the serum specimens of malignant ovarian tumor group (30 cases), benign ovarian diseases (110 cases) and healthy women group (137 cases) were assayed double blindly. Results: 1. The positive rate of HE4 was lower than the one of CA125 in malignant ovarian tumor group (P = 0.036). But HE4 were all negative in benign diseases and healthy women groups. 2. The HE4 assay had advantage over the CA125 assay in receiver operating characteristic-area under the curve (ROC-AUC) and the sensitivity with specificity 100% when comparing ovarian malignance with controls having benign diseases and healthy women, benign tumor or benign diseases groups respectively. 3. Combined assay of HE4 and CA125 was better than CA125 alone when comparing ovarian malignance with controls having any groups. 4. Combined assay was lower in the ROC-AUC and the sensitivity with specificity 100% and higher in the sensitivities with specificity 90% and 95% than HE4 when comparing ovarian cancers with controls having benign tumors or benign diseases groups respectively. 5. The diagnosis efficiency of the HE4 assay at the level 86 pmol/L determined in ROC curve with controls having benign diseases and healthy women group, the 95% reference level 50 pmol/L of healthy women or 150 pmol/L recommended by the kit respectively was compared. Conclusions: The specificity of HE4 assay is higher than CA125 assay in the diagnosis of ovarian cancer and HE4 combined with CA125 assay can improve the diagnoses. The set point of 150 pmol/L had advantage at the accuracy of diagnosis, while the set point of 86 pmol/L had advantage at the screening of malignant ovarian cancer. O515 S100A8/A9 induces apoptosis and inhibits metastasis of human cervical cancer cells CasKi G. Li, F. Qin, Y. Zhang The Ca2+-binding proteins S100A9 and its partner S100A8, were reported to associate with cervical neoplasia. In our previous study, decreased expressions of S100A9 and S100A8 in human cervical squamous cell carcinomausing was showed by serial analysis of gene expression, which was identified by Real Time PCR and Western-blot. Thus, it indicated that S100A8/A9 may play a important role in pathogenesis of cervical cancer. Objective: To investigate the potential functional implications of S100A8 and S100A9 in cervical cancer. Methods: The influence of purified recombinant S100A8 and S100A9 proteins on apoptosis and metastasis in invasive phenotype of a human cervical cancer cell line, CaSki, were investigated. Results: It was showed that S100A8/A9 induced the apoptosis of CaSki cell. In addition, the migratory phenotypes of CaSki cells were significantly inhibited and the expression of matrix metalloproteinase (MMP)-2 was also inhibited.
Conclusions: Our findings show that S100A8 and S100A9 might inhibit carcinoma by inducing the apoptosis of cervical carcinoma cell but inhibiting the metastasis. O516 The study on gene differential expression in ovarian cancer taxol-resistant cells of SKOV3/TAX H. Li. Beijing Shijitan Hospital Background: Ovarian carcinoma is the leading cause of death from gynecological malignancies. Debulking surgery followed by chemotherapy schemes is considered as standard care for those patients so far. However, the acquired drug-resistance from the chemotherapy, which involves multiple factors, represents a major clinical problem. Therefore, elucidating its mechanism and identifying the genes involved in drug-resistance are necessary to improve the effect of chemotherapy, enhancing the outcome of survival. Objective: To investigate the differential expression genes of taxolresistant in ovarian cancer cell line SKOV3/Tax compared with its parental cell SKOV3; to explore the relationship between gene differential expression and Drug-resistance. Methods: Extracting the total RNA from the cell line SKOV3/Tax and its parental cell SKOV3 respectively during the log phase with Trizol one-step method, we purified the RNA with QIAGEN RNeasy kit and then compounded double strand cDNA. Using Affymetrix human U133A cDNA gene chips which include all annotated human genes (22,283 probe sets), we compared the differential gene expression between the SKOV3/TAX and SKOV3 cell line; and analyzed their functions to establish the related genes of drug-resistance. Results: Out of 111 genes of differential expression detected in ovarian cancer, 45 genes expression increased (up-regulated) and 66 genes decreased (down-regulated). The up-regulated genes were CLU, BCL2L1, CD24, POR, BSG, SIRPA, PRKCA, etc; the downregulated genes YES1, CAV1, TGFBI, HSPH1, etc. Conclusions: The genes of differential expression in ovary cancer are related with the mechanism of drug-resistance that involves multiple factors. O517 Screening ovarian cancer serum differential expressing proteins by proteomic techniques H. Li. Beijing Shijitan Hospital Background: Ovarian cancer is the leading cause of gynecological cancer death in worldwide. Due to asymptomatic nature of early stage disease, the majority of the ovarian cancer is diagnosed at the advanced stage. Therefore, increasing the early diagnostic rate is in favor of monitoring clinic treatment and early warning relapse and metastasis, and it is important to improve the ovarian cancer prognosis. At the present time, proteomic techniques provides an vital method for ovarian cancer research. Objective: To detect biomarkers for diagnose and prognosis of ovarian cancer by comparing differential expressing proteins between ovarian cancer and control groups. Methods: (1) Serum from ovarian cancer and healthy control were profiled using two-dimensional gel electrophoresis (2-DE) and analyzed by mass spectrometry. (2) Serum from ovarian cancer and healthy control were profiled using magnetic beads and analyzed by Surface-Enhanced Laser Desorption and Ionization time of flight mass spectrometry (SELDI-TOF-MS). Results: (1) Sixteen different protein points were detected by 2-DE technique, in which there were two specific expression points and seven up-regulation points in cancer group. Mascot search results indicated that the protein points of 7, 12 and 8 were identified to be SAA1, Apo A-IV and Anti-P185-Her2 antibody. (2) Sixty-nine protein peaks were detected at the molecular range of 0 to 50000 by SELDITOF-MS, in which eight protein peaks were different significantly
prevention of sexually transmitted diseases is a stable, harmonious relationship, with a faithful partner. A necessary condition prior to the onset of sexual intercourse is a physical and mental maturity of both partners. It is recommended that the partner who has a urinary or sexually transmitted infection should first recover and then together with the partner maintain normal sexual hygiene. For systematic prevention of sexually transmitted infections diseases in this moment it is necessary: introduce an effective sexual aducation in schools starting from primary school, develop interdisciplinary cooperation between social and medical sciences, including all experts. Stil the best and most important education comes from a healthy family as the core of our society. We want to do something for that young population for education of Human Papillomavirus so we organized High school rock concerts. In appropriate assembly rooms of some Grammar and Secondary School of Zagreb during weekend evenings (Fridays, Saturdays) educational lectures held by medical specialist, were given about sexually transmitted deseases, particularly HPV and after the lectures rock concerts were held by some checked out rock groups and others young so-called "demo" group consisting of secondary school students of Zagreb. Lectures were short (each 20–30 min) accompanied with discussion. Lecture was given in the form of Power Point presentation. By organizing concerts the interest of that population to attend would be greater. Rock concerts after the lecture in some kind of bait for that population to be present at the lecture. By offering free refreshing beverages and media support by radio listened mostly by the young as well as the musical web portal, it has been tried to make popular the whole project. In all given lectures the lecture rooms were too small to accept all audience interested in lecture. Questions made after the lecture were those usual for that age. They asked about the way of contracting HPV, medical treatment of partners and use of contraceptives and aslo about vaccina. Booklets explaining in a popular way the sexually transmitted diseases, way of catching infection and protecting methods were distributed. Mass media have excellently marked the whole project with almost everyday information. We continued with this project also in the this school year. O511 The effectiveness of an educational program for visual inspection with acetic acid (VIA) as cervical cancer screening in Ugandan health care workers: A pilot project L. Levine1 , E. Banks1 , S. Chudnoff1 , K. Taylor2 , C. Kahn1 , C. Eleanor3 . 1 Department of Obstetrics and Gynecology, Montefiore Medical Center, Albert Einstein College of Medicine, 2 Prevention International No Cervical Cancer, 3 Albert Einstein College of Medicine Objectives: To assess a brief, intense, educational intervention implementing VIA amongst diverse healthcare providers. Methods: This is a prospective analysis of an educational modality utilizing a pretest/posttest model. Subjects were physicians, midwives, nurses, and health officers serving women in Kisoro, Uganda recruited via posted announcements and personal invitation. Subjects were surveyed regarding basic demographics and pretested for knowledge in anatomy, cervical dysplasia, cervical cancer, and VIA. Subjects had interactive lectures on these topics and then observed screening by experts and performed screening in a mentored setting. Participants were retested with a validated instrument and technical skills were assessed on live patients at the conclusion of the 5 day workshop. Results: 15 healthcare workers participated in the initial screen and educational modality. The mean scores on the pretest and posttest were 60.9% and 80.3%, respectively (p < 0.001). Scores of the physicians and non-physicians were significantly different at 73.7% and 56.4%, respectively for the pretest (p < 0.01) and 75.2% and
S239
94.0%, p < 0.01 for the posttest. Self-assessment of comfort increased after the training session (p < 0.001). There was a correlation between the participant’s score and the number of times they had seen the procedures. Conclusions: Our program improved knowledge in cervical anatomy and cervical cancer screening using VIA, irrespective of prior training. While level of prior training did correlate with scores on the pretest and posttest, the number of years of experience did not directly correlate with their results. Importantly, the level of comfort after the course did increase in every level of training. O512 Effects of ovarian carcinoma cells on the maturation and function of human dendritic cells C. Li, H. Duan, W. Zhang To investigate the mechanisms of dendritic cells (DC) defective function in tumor that allow them to escape initial immune recognition and destruction, observed the effects of ovarian carcinoma cells on the differentiation, maturation, and function of the mononuclear cell-derived DC in vitro. Ovarian carcinoma cells were used as the conditioned cultural medium for DC culture, while the human bone marrow stromal cells (HFCL) were used as control. And transwell system was used to no intercellular contact culture. The dendritic cells were characterized by phenotypic analysis and evaluated the ability to uptake dextran and stimulate allogeneic T cells of DC. Furthermore, mmune related cytokines were detected. The results show that DC cultured in the presence of ovarian carcinoma cells expressed lower levels of CD1a, MHC-II molecules (HLA-DR) and costimulatory molecules (CD86), and expressed higher CD83 (a typical molecule of mature DC) than that in control group. Moreover, they displayed a much lower ability of phagocytizing dextran and less efficient of inducing allogeneic T-cell proliferation in a mixed lymphocyte reaction, and downregulated IL-12 secretion were also observed. In conclusion, our data suggested ovarian carcinoma cells negatively regulate the production and function of DC by release soluble factors and intercellular contact, could suppress monocyte differentiation into DCs, the most potent antigen-presenting cells (APCs). which might be one of the underlying mechanisms that plays a role in the immune escape of tumor cells. O513 Combination of hysteroscopy and laparoscopy in the treatment of 167 cases of salpingian infertility C. Li, H. Duan, Y. Liu To analyze the clinical value of using hysteroscopy combined with laparoscopy in the diagnosis and treatment of salpingian infertility. 160 cases of salpingian infertility (326 oviducts) performed hysteroscopy combined with laparoscopy, which had been diagnosed by hysterosalpinggography before operation, data was compared and analyzed. In all of the 167 cases (312 abnormal oviducts) diagnosed by hysterosalpinggography, 164 oviducts were not found abnormal (52.56%, 164/312), 4 oviducts were unobstructed but adherence with periphery (1.28%, 4/312), 48 oviducts were interstitial tubal obstruction (15.38%, 48/312), and 96 oviducts were fallopian obstruction with hydrosalpinx (30.77%, 96/312). Otherwise, 2 of 14 normal oviducts diagnosed by hysterosalpinggography were unobstructed but adherence with periphery. The consistency ratio is 49.08% (160/326). Used combination of hysteroscopy and laparoscopy as “golden rule” of diagnosis, the sensitivity of hysterosalpinggography was 98.66%, specificity was 6.82%, positive predictive value was 47.44%, negative predictive value was 85.71%. All the cases of salpingian abnormal were treated during operation, and no complication.
S240
Free communication (oral) presentations / International Journal of Gynecology & Obstetrics 107S2 (2009) S93–S396
In conclusions, hysteroscopy combined with laparoscopy could supply direct diagnosis method for salpingian abnormal, and treat at same time, which is the best way in the diagnosis and treatment of salpingian infertility. O514 The values of serum human epididymis secretory protein 4 and CA125 assay in the diagnosis of ovarian malignance H. Cui1 , D. Li2 , X. Chang3 , X. Ye3 , H. Cheng3 . 1 Gynecologic Oncology Center, Peking University People’ s Hospital, 2 Department of Gynecology and Obstetrics, Beijing Jishuitan Hospital, 3 Gynecologic Oncology Center, Peking University People’s Hospital Objective: To evaluate the value of human epididymis secretory protein 4 (HE4) and CA125 in the diagnosis of ovarian malignance. Methods: HE4 and CA125 in the serum specimens of malignant ovarian tumor group (30 cases), benign ovarian diseases (110 cases) and healthy women group (137 cases) were assayed double blindly. Results: 1. The positive rate of HE4 was lower than the one of CA125 in malignant ovarian tumor group (P = 0.036). But HE4 were all negative in benign diseases and healthy women groups. 2. The HE4 assay had advantage over the CA125 assay in receiver operating characteristic-area under the curve (ROC-AUC) and the sensitivity with specificity 100% when comparing ovarian malignance with controls having benign diseases and healthy women, benign tumor or benign diseases groups respectively. 3. Combined assay of HE4 and CA125 was better than CA125 alone when comparing ovarian malignance with controls having any groups. 4. Combined assay was lower in the ROC-AUC and the sensitivity with specificity 100% and higher in the sensitivities with specificity 90% and 95% than HE4 when comparing ovarian cancers with controls having benign tumors or benign diseases groups respectively. 5. The diagnosis efficiency of the HE4 assay at the level 86 pmol/L determined in ROC curve with controls having benign diseases and healthy women group, the 95% reference level 50 pmol/L of healthy women or 150 pmol/L recommended by the kit respectively was compared. Conclusions: The specificity of HE4 assay is higher than CA125 assay in the diagnosis of ovarian cancer and HE4 combined with CA125 assay can improve the diagnoses. The set point of 150 pmol/L had advantage at the accuracy of diagnosis, while the set point of 86 pmol/L had advantage at the screening of malignant ovarian cancer. O515 S100A8/A9 induces apoptosis and inhibits metastasis of human cervical cancer cells CasKi G. Li, F. Qin, Y. Zhang The Ca2+-binding proteins S100A9 and its partner S100A8, were reported to associate with cervical neoplasia. In our previous study, decreased expressions of S100A9 and S100A8 in human cervical squamous cell carcinomausing was showed by serial analysis of gene expression, which was identified by Real Time PCR and Western-blot. Thus, it indicated that S100A8/A9 may play a important role in pathogenesis of cervical cancer. Objective: To investigate the potential functional implications of S100A8 and S100A9 in cervical cancer. Methods: The influence of purified recombinant S100A8 and S100A9 proteins on apoptosis and metastasis in invasive phenotype of a human cervical cancer cell line, CaSki, were investigated. Results: It was showed that S100A8/A9 induced the apoptosis of CaSki cell. In addition, the migratory phenotypes of CaSki cells were significantly inhibited and the expression of matrix metalloproteinase (MMP)-2 was also inhibited.
Conclusions: Our findings show that S100A8 and S100A9 might inhibit carcinoma by inducing the apoptosis of cervical carcinoma cell but inhibiting the metastasis. O516 The study on gene differential expression in ovarian cancer taxol-resistant cells of SKOV3/TAX H. Li. Beijing Shijitan Hospital Background: Ovarian carcinoma is the leading cause of death from gynecological malignancies. Debulking surgery followed by chemotherapy schemes is considered as standard care for those patients so far. However, the acquired drug-resistance from the chemotherapy, which involves multiple factors, represents a major clinical problem. Therefore, elucidating its mechanism and identifying the genes involved in drug-resistance are necessary to improve the effect of chemotherapy, enhancing the outcome of survival. Objective: To investigate the differential expression genes of taxolresistant in ovarian cancer cell line SKOV3/Tax compared with its parental cell SKOV3; to explore the relationship between gene differential expression and Drug-resistance. Methods: Extracting the total RNA from the cell line SKOV3/Tax and its parental cell SKOV3 respectively during the log phase with Trizol one-step method, we purified the RNA with QIAGEN RNeasy kit and then compounded double strand cDNA. Using Affymetrix human U133A cDNA gene chips which include all annotated human genes (22,283 probe sets), we compared the differential gene expression between the SKOV3/TAX and SKOV3 cell line; and analyzed their functions to establish the related genes of drug-resistance. Results: Out of 111 genes of differential expression detected in ovarian cancer, 45 genes expression increased (up-regulated) and 66 genes decreased (down-regulated). The up-regulated genes were CLU, BCL2L1, CD24, POR, BSG, SIRPA, PRKCA, etc; the downregulated genes YES1, CAV1, TGFBI, HSPH1, etc. Conclusions: The genes of differential expression in ovary cancer are related with the mechanism of drug-resistance that involves multiple factors. O517 Screening ovarian cancer serum differential expressing proteins by proteomic techniques H. Li. Beijing Shijitan Hospital Background: Ovarian cancer is the leading cause of gynecological cancer death in worldwide. Due to asymptomatic nature of early stage disease, the majority of the ovarian cancer is diagnosed at the advanced stage. Therefore, increasing the early diagnostic rate is in favor of monitoring clinic treatment and early warning relapse and metastasis, and it is important to improve the ovarian cancer prognosis. At the present time, proteomic techniques provides an vital method for ovarian cancer research. Objective: To detect biomarkers for diagnose and prognosis of ovarian cancer by comparing differential expressing proteins between ovarian cancer and control groups. Methods: (1) Serum from ovarian cancer and healthy control were profiled using two-dimensional gel electrophoresis (2-DE) and analyzed by mass spectrometry. (2) Serum from ovarian cancer and healthy control were profiled using magnetic beads and analyzed by Surface-Enhanced Laser Desorption and Ionization time of flight mass spectrometry (SELDI-TOF-MS). Results: (1) Sixteen different protein points were detected by 2-DE technique, in which there were two specific expression points and seven up-regulation points in cancer group. Mascot search results indicated that the protein points of 7, 12 and 8 were identified to be SAA1, Apo A-IV and Anti-P185-Her2 antibody. (2) Sixty-nine protein peaks were detected at the molecular range of 0 to 50000 by SELDITOF-MS, in which eight protein peaks were different significantly