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O.629 Sinuslift with chair-side processed mesenchymal stem cells
S158
Journal of Cranio-Maxillofacial Surgery 36(2008) Suppl. 1
Our preliminary results revealed a significantly increased regenerative potential of PDSCs and bone substitutes in contrast to defects reconstructed without PDSCs. The less immunogenic behaviour and cryoconservative properties of PDSCs are important advantages for reconstruction and augmentation purposes in the oral and maxillofacial region. O.628 Silver nanoparticles increased the genes related to healing S. Kim. Sacred Heart Hospital, Anyang, Republic of Korea Objectives: The objective of this study was to examine the effect of the silver nanoparticle application to periodontal ligament cells receiving stress. Methods: Fluid-induced shear stress was used as a stress source. The effect of the silver nanoparticles application was evaluated by cDNA microarray and quantittive RT-PCR. Results: The application of the silver nanoparticles could increase the expression of BMPs, PDGFs, and VEGFs, significantly. The increased expression was confirmed by both methods. Conclusions: This study indicated that the silver nanoparticles might be used as an additive material in the bony defects combined with traditional grafts. O.629 Sinuslift with chair-side processed mesenchymal stem cells S. Sauerbier, R. Gutwald, R. Schmelzeisen. University Hospital Freiburg, Department of Maxillofacial Surgery, Freiburg, Germany Objectives: Oxygen-sensitivity of osteoblasts implies that mesenchymal stem cells (MSCs) are a better source for vitalization of scaffolds because they are able to proliferate under low oxygen tension and differentiate when the oxygen level rises. The aim of the present study is to evaluate the clinical application of chairside processed bone marrow aspirate concentrate including stem cell for augmentation of the edentulous posterior maxilla. Methods: In a randomized controlled clinical trial a sinus floor augmentation of the posterior maxilla was carried out on 75 sinuses (50 test and 25 control). The test group was augmented with mononuclear cells derived from bone marrow aspirate concentrate (BMAC, Harvest Technolgies, Plymouth, MA, USA) on a bovine bone matrix (BioOss, Geistlich Wolhusen, Switzerland). The control group was augmented with bovine bone matrix mixed mixed with autologeous bone. Biopsies were harvested with a trephine burr when dental implants were inserted 3 to 4 months after sinus augmentation. Additionally the obtained cells were analysed for their stemcell character by plastic adherence, differentiation assay, and flowcytometrical-analysis of relevant CD-markers. Results: Histologic and histomorphometric analysis of the obtained specimen showed comparable new bone formation in both groups. In addition, implant survival of the placed dental implants was surveyed. FACS-analysis was positive for CD 44, 73, 90, 105, 166 and negative for CD 34 as for 45. Obtained cells showed the potential to differentiate into adipocytes, chondroblasts and osteoblasts. Conclusions: The results demonstrate the feasibility of this procedure. FACS-analysis and in-vitro tests prove that mesenchymal stem cells were among the mononulcear cells that were transplanted. O.630 Skull reconstruction with rhBMP-2: a never-ending bone bank M. Carstens. Saint Louis University, Saint Louis, USA Calvarial bone is like sandwich. Mesenchymal stem cells (MSCs) are stimulated by dermal neural crest and dural neural crest to
Abstracts, EACMFS XIX Congress produce bilaminar bone. All membranous calvarial bones are of neural crest (NC) origin with the exception of parietal (which contains mesoderm as well). The ability of dura to resynthesize calvarial defects is virtually nil. MSCs of neural crest origin from the neural folds of the CNS contain membrane-bound BMP receptors. At appropriate doseage, rhBMP-2 causes thes cells to proliferated and differentiate into osteoblasts. In Situ Osteogenesis (ISO) using BMP-2 stimulation can generate membranous bone in both children and adults. This permits harvest of customized grafts with assurance of resynthesis. The skull is a “never-ending bone banl”. This series reports on outcomes of 21 calvarial reconstructions using BMP-2 between 2005 and 2007. CT evidence of bone regeneration is routinely demonstrated at 3 months. Complex of virtually any size can be filled. ISO is simple, rapid and avoids morbidity. The BMP “switch procedure” is of particular interest because it permits selective harvest of bone in a fixed geometric shape while simultaneously assuring that the donor site is regenerated. O.631 Tissue engineered bone from adipose derived stem cells R. Suuronen, K. Mesim¨aki, J. Numminen, J. T¨ornwall, M. Rautiainen, C. Lindqvist, B. Lindroos, S. Miettinen. Regea Institute for Regenerative Medicine, Tampere, Finland Human adipose derived stem cells (hASC) provide an attractive source for tissue engineered bone. Here we present our clinical experiences in recostruction of different bony defects of the facial skeleton by using hASC combined with different biomaterial matrices. The laboratory work was carried out in class A clean rooms according to Good Manufacturing Practice (GMP) without any animal-derived materials. The cells were also very carefully characterized. The reconstruction was successfully carried out either in situ or a prefabficated composite was tissue engineered distant to the original defect. The patient characteristics as well as the outcomes of the patients will be described. O.632 Transgenic pig as a potential bone graft donor G.A. Wyszy´nska-Pawelec, J. Zapala. Dept.of Craniomaxillofacial, Oncological and Reconstructive Surg. of the Jagiellonian Univ., Krak´ow, Poland Bone grafts harvested from transgenic pigs, with confirmed integration of human L1,2-fucosylotransferase gene, might be an alternative to autogenous bone grafts in reconstructive surgery of the facial skeleton. The objective of this study was the assessment of changes in transgenic pig’s scapular bone after reconstruction of the defect by autogenous, allogenic and xenogenous bone graft. Material and Methods: 12 transgenic pigs were used in this study. Autogenous bone graft healing was evaluated in 3 animals (I group), xenogenous human bone graft in 6 pigs (II group) and allogenic bone graft harvested from normal pig in 3 animals (III group). Radiological, histological and immunohistochemical evaluation of specimens was performed after 1, 2 and 4 weeks following bone grafting. Results: radiological examination of specimens of the I and II group revealed features of bone graft healing after 4 weeks. Histological examination of the specimens of the I and II group revealed fibrosis and granulation tissue and in the II group presence of new bone trabeculae after 2 weeks. Conclusion: Healing of autogenous and xenogenous human bone grafts inserted into transgenic pigs’ scapula was comparable in radiological and histological examination.
Journal of Cranio-Maxillofacial Surgery 36(2008) Suppl. 1
Our preliminary results revealed a significantly increased regenerative potential of PDSCs and bone substitutes in contrast to defects reconstructed without PDSCs. The less immunogenic behaviour and cryoconservative properties of PDSCs are important advantages for reconstruction and augmentation purposes in the oral and maxillofacial region. O.628 Silver nanoparticles increased the genes related to healing S. Kim. Sacred Heart Hospital, Anyang, Republic of Korea Objectives: The objective of this study was to examine the effect of the silver nanoparticle application to periodontal ligament cells receiving stress. Methods: Fluid-induced shear stress was used as a stress source. The effect of the silver nanoparticles application was evaluated by cDNA microarray and quantittive RT-PCR. Results: The application of the silver nanoparticles could increase the expression of BMPs, PDGFs, and VEGFs, significantly. The increased expression was confirmed by both methods. Conclusions: This study indicated that the silver nanoparticles might be used as an additive material in the bony defects combined with traditional grafts. O.629 Sinuslift with chair-side processed mesenchymal stem cells S. Sauerbier, R. Gutwald, R. Schmelzeisen. University Hospital Freiburg, Department of Maxillofacial Surgery, Freiburg, Germany Objectives: Oxygen-sensitivity of osteoblasts implies that mesenchymal stem cells (MSCs) are a better source for vitalization of scaffolds because they are able to proliferate under low oxygen tension and differentiate when the oxygen level rises. The aim of the present study is to evaluate the clinical application of chairside processed bone marrow aspirate concentrate including stem cell for augmentation of the edentulous posterior maxilla. Methods: In a randomized controlled clinical trial a sinus floor augmentation of the posterior maxilla was carried out on 75 sinuses (50 test and 25 control). The test group was augmented with mononuclear cells derived from bone marrow aspirate concentrate (BMAC, Harvest Technolgies, Plymouth, MA, USA) on a bovine bone matrix (BioOss, Geistlich Wolhusen, Switzerland). The control group was augmented with bovine bone matrix mixed mixed with autologeous bone. Biopsies were harvested with a trephine burr when dental implants were inserted 3 to 4 months after sinus augmentation. Additionally the obtained cells were analysed for their stemcell character by plastic adherence, differentiation assay, and flowcytometrical-analysis of relevant CD-markers. Results: Histologic and histomorphometric analysis of the obtained specimen showed comparable new bone formation in both groups. In addition, implant survival of the placed dental implants was surveyed. FACS-analysis was positive for CD 44, 73, 90, 105, 166 and negative for CD 34 as for 45. Obtained cells showed the potential to differentiate into adipocytes, chondroblasts and osteoblasts. Conclusions: The results demonstrate the feasibility of this procedure. FACS-analysis and in-vitro tests prove that mesenchymal stem cells were among the mononulcear cells that were transplanted. O.630 Skull reconstruction with rhBMP-2: a never-ending bone bank M. Carstens. Saint Louis University, Saint Louis, USA Calvarial bone is like sandwich. Mesenchymal stem cells (MSCs) are stimulated by dermal neural crest and dural neural crest to
Abstracts, EACMFS XIX Congress produce bilaminar bone. All membranous calvarial bones are of neural crest (NC) origin with the exception of parietal (which contains mesoderm as well). The ability of dura to resynthesize calvarial defects is virtually nil. MSCs of neural crest origin from the neural folds of the CNS contain membrane-bound BMP receptors. At appropriate doseage, rhBMP-2 causes thes cells to proliferated and differentiate into osteoblasts. In Situ Osteogenesis (ISO) using BMP-2 stimulation can generate membranous bone in both children and adults. This permits harvest of customized grafts with assurance of resynthesis. The skull is a “never-ending bone banl”. This series reports on outcomes of 21 calvarial reconstructions using BMP-2 between 2005 and 2007. CT evidence of bone regeneration is routinely demonstrated at 3 months. Complex of virtually any size can be filled. ISO is simple, rapid and avoids morbidity. The BMP “switch procedure” is of particular interest because it permits selective harvest of bone in a fixed geometric shape while simultaneously assuring that the donor site is regenerated. O.631 Tissue engineered bone from adipose derived stem cells R. Suuronen, K. Mesim¨aki, J. Numminen, J. T¨ornwall, M. Rautiainen, C. Lindqvist, B. Lindroos, S. Miettinen. Regea Institute for Regenerative Medicine, Tampere, Finland Human adipose derived stem cells (hASC) provide an attractive source for tissue engineered bone. Here we present our clinical experiences in recostruction of different bony defects of the facial skeleton by using hASC combined with different biomaterial matrices. The laboratory work was carried out in class A clean rooms according to Good Manufacturing Practice (GMP) without any animal-derived materials. The cells were also very carefully characterized. The reconstruction was successfully carried out either in situ or a prefabficated composite was tissue engineered distant to the original defect. The patient characteristics as well as the outcomes of the patients will be described. O.632 Transgenic pig as a potential bone graft donor G.A. Wyszy´nska-Pawelec, J. Zapala. Dept.of Craniomaxillofacial, Oncological and Reconstructive Surg. of the Jagiellonian Univ., Krak´ow, Poland Bone grafts harvested from transgenic pigs, with confirmed integration of human L1,2-fucosylotransferase gene, might be an alternative to autogenous bone grafts in reconstructive surgery of the facial skeleton. The objective of this study was the assessment of changes in transgenic pig’s scapular bone after reconstruction of the defect by autogenous, allogenic and xenogenous bone graft. Material and Methods: 12 transgenic pigs were used in this study. Autogenous bone graft healing was evaluated in 3 animals (I group), xenogenous human bone graft in 6 pigs (II group) and allogenic bone graft harvested from normal pig in 3 animals (III group). Radiological, histological and immunohistochemical evaluation of specimens was performed after 1, 2 and 4 weeks following bone grafting. Results: radiological examination of specimens of the I and II group revealed features of bone graft healing after 4 weeks. Histological examination of the specimens of the I and II group revealed fibrosis and granulation tissue and in the II group presence of new bone trabeculae after 2 weeks. Conclusion: Healing of autogenous and xenogenous human bone grafts inserted into transgenic pigs’ scapula was comparable in radiological and histological examination.