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O72. Study of oral micro nucleated cell frequency in oral submucous fibrosis patients – A case control study
Abstracts / Oral Oncology 47 (2011) S28–S73
longed, chronic iron and/or vitamin B complex deficiency. Theory like the seed and soil which explains both aspects of local irritants and the altered oral mucosa following nutritional deficiency needs to be reemphasized to explain the pathogenesis. Hence aim is to estimate the levels of serum and RBC folic acid in patients with OSMF and also to estimate the same in patients with habits without OSMF. Methods: Thirty histologically confirmed cases of OSMF (study group) and 30 individuals with habits but without overt disease were included in the study (control group). Results and conclusion: Serum folic acid levels noted in 76% of study group 0–4 ng per ml and 23% were more than 4 ng. In the control group all were more than 4 ng. RBC folic acid levels noted in 100% of study group were less than 145 ng per ml where as only 10% were less than 145 ng per ml in control group. Deficiency of folic acid may cause anemia, alterations in oral mucosa making it more susceptible for the action of local irritants and the reduction of which also causes inactivation of enzyme collagenase leading to fibrosis. Hence in our present study there was significant reduction in the folic acid levels in study group as compared to control group. doi:10.1016/j.oraloncology.2011.06.181
S53
pouches per day. Frequency of MNC and MN were increased with clinical staging of Type I to IV. The frequency of MNC and MN in OSMF patients ranged from 0.6% to 5.3% and 0.50% to 5.74%, where as in control group ranged from 0.5% to 0.9% and 0.22% to 0.88%, respectively. Using a paired-t test, it was found that both MNC and MN in OSMF patients were statistically significantly elevated (p < 0.05) as compared to control group. Mean percentage frequencies were higher on chewing site than on non-chewing site of buccal mucosa. Discussion: Desai et al. (1996) showed a highly significant increase in the mean MNC in premalignancy as compared to their control group. Similar results were also seen in study done by Rashmi Saran et al. (2008). In the present study an increase in MNC & MN in OSMF patients compared to healthy individuals and is highly beneficial to check the progress of premalignancy to squamous cell carcinoma followed by its early treatment. In view of the fact that gutkha chewing is practiced comparatively at younger age and they developed OSMF in shorter duration, there may be increased risk of malignant development in 3rd and 4th decade. Thus MN test can be used as a reliable marker to assess the genotoxicity and for the early diagnosis of premalignancy and malignancy.
doi:10.1016/j.oraloncology.2011.06.183 O71. Incidence of occult node metastasis in clinically No Neck for alveolar carcinoma during supraomohyoid neck dissection S. Elengkumaran *, K.S. Sabitha, M. Vijayakumar, N. Nadimulhoda, D.C. Rajani, K. Trupti Kidwai Memorial Institute of Oncology, India This retrospective study was done at Kidwai Memorial Institute by the Department of Oral Oncology to know the effect of clinicopathologic factors on local tumor control and survival in patients with mandibular alveolar carcinoma. Patients with mandibular alveolar carcinoma with clinically No neck were treated surgically with hemimandibulectomy or marginal mandibulectomy with supraomohyoid neck dissection. In this study patients treated from 2008 Jan to 2010 Dec were included, the incidence of metastasis, clinicopathologic variables on local tumor control and patient survival was assessed. doi:10.1016/j.oraloncology.2011.06.182
O72. Study of oral micro nucleated cell frequency in oral submucous fibrosis patients – A case control study H. Kaveri *, K. Anila SDM College of Dental Sciences and Hospital, India Introduction: The purpose of this study was to determine the frequency of micronucleated cells (MNCs) and micronuclei (MN) in oral submucous fibrosis (OSMF) patients and healthy individuals. Methods: Twenty patients with OSMF and 20 age–gender matched healthy individuals were included in the study. A detailed history of each patient was recorded along with a clinical examination. Exfoliated cells were obtained by scraping from right and left buccal mucosa and were screened for MNC and MN. Frequencies of MNC and MN were compared between OSMF and control group. Comparison was made between chewing site and non-chewing site of buccal mucosa in OSMF patients. Results: Eighteen patients (18/20) chewed gutkha and developed symptoms of OSMF within 1–2 years with frequency of 11–20
O73. Abnormalities in periodontal and salivary tissues in conditional presenilin 1 and presenilin 2 conditional double knockout mice W. Han *,a, T. Ji b, L. Wang b, L. Yan b, B. Mei b, J. Su a a
School of Stomatology, Tongji University, Shanghai, China Shanghai Key Laboratory of Brain Functional Genomics, Key Laboratory of Brain Functional Genomics, Ministry of Education, East China Normal University, Shanghai, China
b
Introduction: We used forebrain specific conditional presenilin 1 (PS1) and presenilin 2 (PS2) double knockout mice (dKO mice), which exhibit neurodegenerative disease-like symptoms, including inflammation of the brain and periphery, to investigate whether periodontal and salivary tissues display alterations. Methods: Mandibles were dissected for alveolar bone height analysis. Maxillae were fixed and decalcified for histological observation and osteoclast detection. Submandibular glands were fixed for histological observation. The submandibular gland and the gingiva of the mandibular incisor teeth were used to assay inflammatory mediators. Results: At 9 months, the number of osteoclasts had significantly increased in the periodontal ligament and the periodontal tissues exhibited obvious histomorphological abnormalities in the dKO mice compared to the control mice at the same age. Alveolar bone loss in dKO mice increased with age. The salivary tissues in dKO mice exhibited obvious age-dependent histomorphological abnormalities. The levels of the inflammatory mediators IL-1beta, TNF-alpha and GM-CSF in the submandibular gland and gingiva also increased in an age-dependent manner. Discussion: These findings suggest that inflammation in the dKO brain could expand to the periphery, including the oral tissue, which could ultimately induce abnormalities in the periodontal and salivary tissues. doi:10.1016/j.oraloncology.2011.06.184
longed, chronic iron and/or vitamin B complex deficiency. Theory like the seed and soil which explains both aspects of local irritants and the altered oral mucosa following nutritional deficiency needs to be reemphasized to explain the pathogenesis. Hence aim is to estimate the levels of serum and RBC folic acid in patients with OSMF and also to estimate the same in patients with habits without OSMF. Methods: Thirty histologically confirmed cases of OSMF (study group) and 30 individuals with habits but without overt disease were included in the study (control group). Results and conclusion: Serum folic acid levels noted in 76% of study group 0–4 ng per ml and 23% were more than 4 ng. In the control group all were more than 4 ng. RBC folic acid levels noted in 100% of study group were less than 145 ng per ml where as only 10% were less than 145 ng per ml in control group. Deficiency of folic acid may cause anemia, alterations in oral mucosa making it more susceptible for the action of local irritants and the reduction of which also causes inactivation of enzyme collagenase leading to fibrosis. Hence in our present study there was significant reduction in the folic acid levels in study group as compared to control group. doi:10.1016/j.oraloncology.2011.06.181
S53
pouches per day. Frequency of MNC and MN were increased with clinical staging of Type I to IV. The frequency of MNC and MN in OSMF patients ranged from 0.6% to 5.3% and 0.50% to 5.74%, where as in control group ranged from 0.5% to 0.9% and 0.22% to 0.88%, respectively. Using a paired-t test, it was found that both MNC and MN in OSMF patients were statistically significantly elevated (p < 0.05) as compared to control group. Mean percentage frequencies were higher on chewing site than on non-chewing site of buccal mucosa. Discussion: Desai et al. (1996) showed a highly significant increase in the mean MNC in premalignancy as compared to their control group. Similar results were also seen in study done by Rashmi Saran et al. (2008). In the present study an increase in MNC & MN in OSMF patients compared to healthy individuals and is highly beneficial to check the progress of premalignancy to squamous cell carcinoma followed by its early treatment. In view of the fact that gutkha chewing is practiced comparatively at younger age and they developed OSMF in shorter duration, there may be increased risk of malignant development in 3rd and 4th decade. Thus MN test can be used as a reliable marker to assess the genotoxicity and for the early diagnosis of premalignancy and malignancy.
doi:10.1016/j.oraloncology.2011.06.183 O71. Incidence of occult node metastasis in clinically No Neck for alveolar carcinoma during supraomohyoid neck dissection S. Elengkumaran *, K.S. Sabitha, M. Vijayakumar, N. Nadimulhoda, D.C. Rajani, K. Trupti Kidwai Memorial Institute of Oncology, India This retrospective study was done at Kidwai Memorial Institute by the Department of Oral Oncology to know the effect of clinicopathologic factors on local tumor control and survival in patients with mandibular alveolar carcinoma. Patients with mandibular alveolar carcinoma with clinically No neck were treated surgically with hemimandibulectomy or marginal mandibulectomy with supraomohyoid neck dissection. In this study patients treated from 2008 Jan to 2010 Dec were included, the incidence of metastasis, clinicopathologic variables on local tumor control and patient survival was assessed. doi:10.1016/j.oraloncology.2011.06.182
O72. Study of oral micro nucleated cell frequency in oral submucous fibrosis patients – A case control study H. Kaveri *, K. Anila SDM College of Dental Sciences and Hospital, India Introduction: The purpose of this study was to determine the frequency of micronucleated cells (MNCs) and micronuclei (MN) in oral submucous fibrosis (OSMF) patients and healthy individuals. Methods: Twenty patients with OSMF and 20 age–gender matched healthy individuals were included in the study. A detailed history of each patient was recorded along with a clinical examination. Exfoliated cells were obtained by scraping from right and left buccal mucosa and were screened for MNC and MN. Frequencies of MNC and MN were compared between OSMF and control group. Comparison was made between chewing site and non-chewing site of buccal mucosa in OSMF patients. Results: Eighteen patients (18/20) chewed gutkha and developed symptoms of OSMF within 1–2 years with frequency of 11–20
O73. Abnormalities in periodontal and salivary tissues in conditional presenilin 1 and presenilin 2 conditional double knockout mice W. Han *,a, T. Ji b, L. Wang b, L. Yan b, B. Mei b, J. Su a a
School of Stomatology, Tongji University, Shanghai, China Shanghai Key Laboratory of Brain Functional Genomics, Key Laboratory of Brain Functional Genomics, Ministry of Education, East China Normal University, Shanghai, China
b
Introduction: We used forebrain specific conditional presenilin 1 (PS1) and presenilin 2 (PS2) double knockout mice (dKO mice), which exhibit neurodegenerative disease-like symptoms, including inflammation of the brain and periphery, to investigate whether periodontal and salivary tissues display alterations. Methods: Mandibles were dissected for alveolar bone height analysis. Maxillae were fixed and decalcified for histological observation and osteoclast detection. Submandibular glands were fixed for histological observation. The submandibular gland and the gingiva of the mandibular incisor teeth were used to assay inflammatory mediators. Results: At 9 months, the number of osteoclasts had significantly increased in the periodontal ligament and the periodontal tissues exhibited obvious histomorphological abnormalities in the dKO mice compared to the control mice at the same age. Alveolar bone loss in dKO mice increased with age. The salivary tissues in dKO mice exhibited obvious age-dependent histomorphological abnormalities. The levels of the inflammatory mediators IL-1beta, TNF-alpha and GM-CSF in the submandibular gland and gingiva also increased in an age-dependent manner. Discussion: These findings suggest that inflammation in the dKO brain could expand to the periphery, including the oral tissue, which could ultimately induce abnormalities in the periodontal and salivary tissues. doi:10.1016/j.oraloncology.2011.06.184