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Observations on the biochemical composition of the enteropneust Ptychodera flava
Vol. 7, Part II, pp . 829-832, 1988 . Life Sciences Printed in Great Britain.
Pergamon Press
OBSERVATIONS ON THE BIOCHEMICAL CU~iPOSITION Of TüE ENTERUYNEUS'f YTYCHOUERA FLAVA S . Kriahnan and Y . Govindarajulu Department of Loology, Medurai University, Madurai-L, S . India
(Received 27 March 1988)
EVEN though the morphology, biology and development of enteropneusta have been studied by many (1, 2, 9), the biochemical composition has not been reported so far, save for a paper on the chemical composition of UalanoAlosaua Aitgaa by Ue Jorge et al . (4) . In this paper, the authors have investigated in detail the inorganic salts and elements present . lhey also give the total nitrogen values for different parts of the animal . The present report deals with the biochemical composition of }'tychodera flavor , collected from t}~e gulf of Ptannar area, on the east coast of lndia . for analyses, animals were divided into three regions, 1 . proboscis, collar and genital region, II . hepatic region and III . abdominal region . Proteins and lipids were estimated by the modi fied biuret method and chloroform-methanol method respectively, as described by ltaymont et aI . (5) . Cart~ohydrates were analysed using the phenol-aul}~huric enid method of Uuboia et al . (6) end the nucleic acids, by tt~e SFhimidt-'fhannhauser-Schneider method as outlined by Glick (7) . T}ie results are expressed as percenCages of dry weight . The values
obtained for water protein, carbohydrates, lipids
and nucleic acids are presented in Table 1 .
The water content of
different parts of the animal 'varies from 7L .01-79 .01% with a
829
Vol . 7, No. 12
BIOCHEMICAL COMP06ITION
630
mean value of 75 .319îo.
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Vol . 7, No . 12
BIOCHEMICAL COMPO6ITION
831
The amount of protein varies from 39 .25-60 .00°n of the dry weight, ~rhsn all the results of different regions are pooled together . The protein content is reported to vary from 16-5496 in the tunicate Ciona intestinalis (8) .
However, in the present form,
when the three regions are taken into consideration separately, there is a slight variation . The lipid content of P . flava shows a range of 11 .85-22 .909tî of the dry weight, with a mean value of 17 .719é . In tunicetea, the values vary from 4-10 .39L in the different tissues studied (8) . The total carbohydrates are
low, ranging from 3 .16-10 .639 : of the
dry weight, with a mean value of 6 .769îi . While 1tNA ranges from 0 .37-1 .7696 with a mean value of 1 .U$9 :, UNA shows a range of O .lZU .669~, with a mean value of 0 .47% . The variations noticed in the constituents may be attributed to the animals being in different stages of maturity . When all the results are considered, one notices a greater accumulation of car bohydrates, lip~dsand nucleic acids in theregiona I and IlI i .e ., the anterior an~1 posterior regions of the body, th~la suggesting the existence of a gradient in the distribution of these substances . Acknowledgments We are very grateful to Prof . S . 1Crishhaawamy, Department of Zoology, Nadurai University, Dladurai for suggesting the problem and valuable guidance . One of us (S .K .) acknowledges his indebtedness to the Council of Scientific and Industrial research, New Delhi, India for providing a Junior Research fellowship to carry out these investigations . references 1 . C . Iturdon-Jones, Phil . Trance . Koy . Soc . li, ~, 553 (1952) " 2 . h .W . Knight-Jones, lbid ,-'~36, 315 (ly5z) .
892
BIOCHEMICAL COMP03ITION
Vol. 7, No . 12
3 . P . Sewaya, Nature , ~, 730 (1951) . 4 . F .B . lle Jorge,J .A . Peteraen and P .~.Séwaya, Comp, ßiochem . Phyaiol , ~, a77 (1967) . 5 . J .Q .G . Raymont, J . Austin and ~ . Liaiford .
J . Cons . Int . kxplor .
Mer, ~, 354 (1964) . 6 . Dl . Dubois, K .A . Gilles, J .K . Hamilton, P .A . Robers and J . Bannet, Anal . Chem .,
8, 3$ .0 (956) .
7. D .Glick, Methode of Biochemical Analysis p . 287, Publishers, New York (1957) . 8 . A .C . Giese, Phyalol .Rev ., !6, 244 (1966) .
Interacience
Pergamon Press
OBSERVATIONS ON THE BIOCHEMICAL CU~iPOSITION Of TüE ENTERUYNEUS'f YTYCHOUERA FLAVA S . Kriahnan and Y . Govindarajulu Department of Loology, Medurai University, Madurai-L, S . India
(Received 27 March 1988)
EVEN though the morphology, biology and development of enteropneusta have been studied by many (1, 2, 9), the biochemical composition has not been reported so far, save for a paper on the chemical composition of UalanoAlosaua Aitgaa by Ue Jorge et al . (4) . In this paper, the authors have investigated in detail the inorganic salts and elements present . lhey also give the total nitrogen values for different parts of the animal . The present report deals with the biochemical composition of }'tychodera flavor , collected from t}~e gulf of Ptannar area, on the east coast of lndia . for analyses, animals were divided into three regions, 1 . proboscis, collar and genital region, II . hepatic region and III . abdominal region . Proteins and lipids were estimated by the modi fied biuret method and chloroform-methanol method respectively, as described by ltaymont et aI . (5) . Cart~ohydrates were analysed using the phenol-aul}~huric enid method of Uuboia et al . (6) end the nucleic acids, by tt~e SFhimidt-'fhannhauser-Schneider method as outlined by Glick (7) . T}ie results are expressed as percenCages of dry weight . The values
obtained for water protein, carbohydrates, lipids
and nucleic acids are presented in Table 1 .
The water content of
different parts of the animal 'varies from 7L .01-79 .01% with a
829
Vol . 7, No. 12
BIOCHEMICAL COMP06ITION
630
mean value of 75 .319îo.
w
m
0
m
b
0 m
0
U
m
U
++
k m m 4
w .a
++ kd
a
s.
E o U
4 F
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Vol . 7, No . 12
BIOCHEMICAL COMPO6ITION
831
The amount of protein varies from 39 .25-60 .00°n of the dry weight, ~rhsn all the results of different regions are pooled together . The protein content is reported to vary from 16-5496 in the tunicate Ciona intestinalis (8) .
However, in the present form,
when the three regions are taken into consideration separately, there is a slight variation . The lipid content of P . flava shows a range of 11 .85-22 .909tî of the dry weight, with a mean value of 17 .719é . In tunicetea, the values vary from 4-10 .39L in the different tissues studied (8) . The total carbohydrates are
low, ranging from 3 .16-10 .639 : of the
dry weight, with a mean value of 6 .769îi . While 1tNA ranges from 0 .37-1 .7696 with a mean value of 1 .U$9 :, UNA shows a range of O .lZU .669~, with a mean value of 0 .47% . The variations noticed in the constituents may be attributed to the animals being in different stages of maturity . When all the results are considered, one notices a greater accumulation of car bohydrates, lip~dsand nucleic acids in theregiona I and IlI i .e ., the anterior an~1 posterior regions of the body, th~la suggesting the existence of a gradient in the distribution of these substances . Acknowledgments We are very grateful to Prof . S . 1Crishhaawamy, Department of Zoology, Nadurai University, Dladurai for suggesting the problem and valuable guidance . One of us (S .K .) acknowledges his indebtedness to the Council of Scientific and Industrial research, New Delhi, India for providing a Junior Research fellowship to carry out these investigations . references 1 . C . Iturdon-Jones, Phil . Trance . Koy . Soc . li, ~, 553 (1952) " 2 . h .W . Knight-Jones, lbid ,-'~36, 315 (ly5z) .
892
BIOCHEMICAL COMP03ITION
Vol. 7, No . 12
3 . P . Sewaya, Nature , ~, 730 (1951) . 4 . F .B . lle Jorge,J .A . Peteraen and P .~.Séwaya, Comp, ßiochem . Phyaiol , ~, a77 (1967) . 5 . J .Q .G . Raymont, J . Austin and ~ . Liaiford .
J . Cons . Int . kxplor .
Mer, ~, 354 (1964) . 6 . Dl . Dubois, K .A . Gilles, J .K . Hamilton, P .A . Robers and J . Bannet, Anal . Chem .,
8, 3$ .0 (956) .
7. D .Glick, Methode of Biochemical Analysis p . 287, Publishers, New York (1957) . 8 . A .C . Giese, Phyalol .Rev ., !6, 244 (1966) .
Interacience