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Occurrence of canavanine in the mature seeds from Vicia ervilia and Vicia villosa
Poster Session II
II-ii
( 6 - 7 March 1981)
A39
Occurrence of tansy@nine in the mature seeds from Viola ervllis
Viois v i l r d n . I.
Ferraado!
N| Ao Garc~s!
and
N. C u a r t e r o and E. B a l l e s t e r o s o
D e p a r t m e n t of P h y s i o l o g i c a l S c i e n c e s . C s t h e d r e of P h a r m a c o l o g y end Toxicology. Faculty of Veterinary. University of Zaragozso Zaragozao Spain. Cansvanine, the oxigusnidino structure occuring in nature, which Is analogous to the L-arglnine, is contained in the seedsof some l e g u ~ n o u s plants. Thls compound acts as an arglnlne autagonlst in many micrDorganlams. Histological changes were observed In the 11vers of rats after both, chronic end acute experiments. The content of canavanine Is detected and quantified In different e~ tracts of the seeds of Vlcls ervllla and VIola vlllosa with a colorl metric assay and modifying the procedures of Bell and Rosenthsl. Init the canevanlne reacts with trisodium pentacyanoammonio ferrate at pH 7. The reagent have been prepared according to the method of Feeton and activated with ultraviolet light° After having deleted the seeds the extraction have been done with ethanol 30%,-7C~,-1(~ HCI i N, acetone-5% HCI 6 N, methil, ethil ketone-5% HCI 6 N end n-butanol satured with HCI i N. Also ethanolic extracts have been ~urifled with acetate basic of Pb. The extracts with the highest yield hewe been the one done with ethanol-lO% HCI i N giving 150 mg of canavanine from 1OO ~ of V. ervilia seeds and 584 mg from the same quantity of V. vllloss seeds. The one done with methll, ethil, ketone end n-butanol gave negative response to the test-assay.
11-12
EFFECTS
OFPHENOBARBITAL
BIOCHEMICAL
CHANGES
PENTTI ARVELA, University Alterations
AND ~-NAPHTOFLAVONE
ON CERIUM INDUCED
IN RAT SERUM
Ph.D., Matti Reinil~,
M.Sc.and Olavi Pelkonen M.D.
of Oulu, Oulu, Finlan~ in serum biochemical
constituents
has been observed
in rats during liver damage caused by intravenous of light lanthanons. In the present clinical
study we have investigated
serum parameters
administration
which of the well known
are changed after experimental
liver le-
sion induced by the light lanthanen,
cerium (Ce), and can these
changes be normalized
rats with commonly used me-
tabolic (NP).
inducers,
PB pretreatment
by pretreating
such as phenobarbital
normalized
the elevation
aphos and crea but the induction
(PB) or ~-naphtoflavone of rat serum alat, asat,
effect of NP was less pronounced.
No changes were observed in serum albumin, transferase
or in the concentration
tion of bilirubin was potentiated
urea,
gammaglutamyl-
of K, Na, Ca or P. The eleva-
by pretreatment
with PB but not
with NP. Our results show that the known cytochrome P-448 inducer,NP, has less effect in normalizing liver lesions than P-450 inducer, PB.
II-ii
( 6 - 7 March 1981)
A39
Occurrence of tansy@nine in the mature seeds from Viola ervllis
Viois v i l r d n . I.
Ferraado!
N| Ao Garc~s!
and
N. C u a r t e r o and E. B a l l e s t e r o s o
D e p a r t m e n t of P h y s i o l o g i c a l S c i e n c e s . C s t h e d r e of P h a r m a c o l o g y end Toxicology. Faculty of Veterinary. University of Zaragozso Zaragozao Spain. Cansvanine, the oxigusnidino structure occuring in nature, which Is analogous to the L-arglnine, is contained in the seedsof some l e g u ~ n o u s plants. Thls compound acts as an arglnlne autagonlst in many micrDorganlams. Histological changes were observed In the 11vers of rats after both, chronic end acute experiments. The content of canavanine Is detected and quantified In different e~ tracts of the seeds of Vlcls ervllla and VIola vlllosa with a colorl metric assay and modifying the procedures of Bell and Rosenthsl. Init the canevanlne reacts with trisodium pentacyanoammonio ferrate at pH 7. The reagent have been prepared according to the method of Feeton and activated with ultraviolet light° After having deleted the seeds the extraction have been done with ethanol 30%,-7C~,-1(~ HCI i N, acetone-5% HCI 6 N, methil, ethil ketone-5% HCI 6 N end n-butanol satured with HCI i N. Also ethanolic extracts have been ~urifled with acetate basic of Pb. The extracts with the highest yield hewe been the one done with ethanol-lO% HCI i N giving 150 mg of canavanine from 1OO ~ of V. ervilia seeds and 584 mg from the same quantity of V. vllloss seeds. The one done with methll, ethil, ketone end n-butanol gave negative response to the test-assay.
11-12
EFFECTS
OFPHENOBARBITAL
BIOCHEMICAL
CHANGES
PENTTI ARVELA, University Alterations
AND ~-NAPHTOFLAVONE
ON CERIUM INDUCED
IN RAT SERUM
Ph.D., Matti Reinil~,
M.Sc.and Olavi Pelkonen M.D.
of Oulu, Oulu, Finlan~ in serum biochemical
constituents
has been observed
in rats during liver damage caused by intravenous of light lanthanons. In the present clinical
study we have investigated
serum parameters
administration
which of the well known
are changed after experimental
liver le-
sion induced by the light lanthanen,
cerium (Ce), and can these
changes be normalized
rats with commonly used me-
tabolic (NP).
inducers,
PB pretreatment
by pretreating
such as phenobarbital
normalized
the elevation
aphos and crea but the induction
(PB) or ~-naphtoflavone of rat serum alat, asat,
effect of NP was less pronounced.
No changes were observed in serum albumin, transferase
or in the concentration
tion of bilirubin was potentiated
urea,
gammaglutamyl-
of K, Na, Ca or P. The eleva-
by pretreatment
with PB but not
with NP. Our results show that the known cytochrome P-448 inducer,NP, has less effect in normalizing liver lesions than P-450 inducer, PB.