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Old Dogmas and New Insides About Effects of Oxidative Stress on Human Health: Vitality and Physical Performance Test
Nutrition & Health Strawberry Enriched Diet Counteracts Oxidative Stress in Plasma and Liver of Old Rats and Improves Mitochondrial Functionality Francesca Giampieri1, Josè M Alvarez-Suarez1, Luca Mazzoni1, Massimiliano Gasparrini1, Tamara Y Forbes-Hernandez1, Bruno Mezzetti1, and Maurizio Battino1 1 Università Politecnica delle Marche, Ancona, Italy Aging, the result of the build-up of oxidative damage throughout life and of the ability to resist or to prevent oxidative stress, is a key determinant of longevity. the impact of the diet and dietary components on aging has been widely recognised and the antioxidant supplementation may be used as dietary antiaging therapies. Therefore, a diet rich in fruits with high content of antioxidants, as strawberry,1-3 could be extremely beneficial in slowing the aging phenotype. the aim of the present work was to assess the effects of a long term diet enriched in strawberry against oxidative stress in old rats. the potential changes in plasma, liver and mitochondria functionality were evaluated. the strawberry supplementation increased plasma total antioxidant capacity (+41%) and HDL-chol (+25.3%), decreased plasma ROS production (-16%) and LDL-chol (-19.5%) compared to control group. the berry enriched diet protected plasma and liver proteins and lipids: protein carbonyls decreased markedly in plasma (47.4%) and liver (-62.8%) of old rats fed with strawberry; the same occurred with TBARS in plasma (-25.8%) and liver (57.7%). in liver mitochondria, strawberry diet led to an important decrease (-39.1%) in ROS levels, to a marked improvement (+14.1%) in basal mitochondria respiratory performance and to a relevant increase in lypophilic antioxidants as vitamin E (+52%) and Coenzyme Q (+12%). Our results confirm the potential health benefit of strawberry fruit against oxidative stress, also in physiological conditions characterized by a higher level of oxidative stress as aging. References 1. Giampieri F et al, Nutrition 2012, 28:9-19 2. Giampieri F et al, J Agric Food Chem 2012, 60:2322-7 3. Diamanti J et al, J Berry Res 2010, 1:103-14
doi: 10.1016/j.freeradbiomed.2013.10.661
Iron and Complementary Feeding of Breast-Fed Infants James Kenneth Friel1, Wafaa Qasem1, Chenxi Cai1, Zakir Hossain1, Sarah Jorgensen1, Bill Zackert2, and L Jackson Roberts II2 1 University of Manitoba, Canada, 2Vanderbilt University, United States Rationale: the American Pediatric Society and Health Canada both recommend that iron fortified cereal (most iron unabsorbed) or meat (most iron absorbed) be introduced to all breast-fed infants at six months of age to support iron stores and linear growth. Evidence suggests that the iron contained in these foods, particularly cereal, is not fully absorbed and will collect in the colon with the possibility of free radical generation and intestinal inflammation.
doi: 10.1016/j.freeradbiomed.2013.10.660
Objectives: to assess the safety of the recommended first solid food for exclusively breast-fed infants from a free radical perspective.
Old Dogmas and New Insides About Effects of Oxidative Stress on Human Health: Vitality and Physical Performance Test Bruno Fink1, Nelli Fink1, and Boris Nemzer2 1 Noxygen Science Transfer & Diagnostics GmbH, Germany, 2Van Drunen Farms, FutureCeuticals Inc., United States Research of last two decades described oxidative stress as key factor in development and progression of different nation disorders as myocardial infarct, diabetes etc. Formation of reactive oxygen species (ROS), if not appropriately counteracted, leads to oxidative stress and cellular damage. the objective of this pilot study was to investigate effects of in vivo formation of ROS during moderate exercise training as well as during supplementation of mineral and antioxidant formulation elevATPTM or in combination of both.
6
Healthy volunteers used moderate exercised for 20 min or ingested single dose of elevATPTM or combination of both to simulate different level of ROS generation. Employed Vitality & Physical Performance test reflecting metabolic and respiratory activity of cells and production of ROS in vivo. the test was performed in capillary blood using EPR spectrometer E-SCAN under resting conditions and after short-term exercise challenge with or without elevATPTM supplementation. to the same extent we observed changes of cardiac hemodynamic parameters and other metabolic as well as inflammatory blood parameters. In this pilot study we found that moderate exercise as well as exercise challenge inducing formation of ROS up to 25% from resting value. in opposite to that we found up to 20 % decrease in the production of ROS in human volunteers after supplementation of single dose of elevATPTM formulation. in the same time we observed progressive increase in respiratory and metabolic activity of blood cells after moderate exercise and elevATPTM supplementation. Finally we found that extent of compensatory mechanisms were potentiated after combination of moderate exercise with supplementation of elevATPTM formulation. Results of this pilot study pointing on potential discovery of novel mechanism in regulation/adaptation of human body to the shortterm elevation of intracellular generation of ROS! This ongoing research continues to offer new perspectives on the creation of new formulations for the future supplementation to groups exposed to oxidative stress such as excessive exercise, metabolic and cardiovascular disorders, smoking, mental stress.
Design and methods: Ninety exclusively breastfed infants were randomized to 1 of 3 feeding groups: iron fortified cereal, iron fortified cereal with fruit or meat. Urine and stool samples were collected while exclusively breastfeeding and before introduction of solid foods (4-5.5 months) and 3 weeks after introduction of these foods to assess the following markers: urinary F2 isoprostanes (LC-MS-MS), urinary 8OH-deoxy guanosine (ELISA), fecal calprotectin (ELISA), reactive oxygen species (ROS-HPLC) and non-heme iron generation (Colorimetric) in the stool, and the fecal microbiome (16S RNA gene pyrosequencing). Results: preliminary results are presented for F2-isoprostanes and ROS generation: neither ROS generation (baseline: 0.031±0.019; after feeds 0.031 ±0.015 RU; n=12) nor urinary isoprostanes (baseline: 10.1±7.2; after feeds 12.8 ±7.6 ng/mg
6)5%0
doi: 10.1016/j.freeradbiomed.2013.10.661
Iron and Complementary Feeding of Breast-Fed Infants James Kenneth Friel1, Wafaa Qasem1, Chenxi Cai1, Zakir Hossain1, Sarah Jorgensen1, Bill Zackert2, and L Jackson Roberts II2 1 University of Manitoba, Canada, 2Vanderbilt University, United States Rationale: the American Pediatric Society and Health Canada both recommend that iron fortified cereal (most iron unabsorbed) or meat (most iron absorbed) be introduced to all breast-fed infants at six months of age to support iron stores and linear growth. Evidence suggests that the iron contained in these foods, particularly cereal, is not fully absorbed and will collect in the colon with the possibility of free radical generation and intestinal inflammation.
doi: 10.1016/j.freeradbiomed.2013.10.660
Objectives: to assess the safety of the recommended first solid food for exclusively breast-fed infants from a free radical perspective.
Old Dogmas and New Insides About Effects of Oxidative Stress on Human Health: Vitality and Physical Performance Test Bruno Fink1, Nelli Fink1, and Boris Nemzer2 1 Noxygen Science Transfer & Diagnostics GmbH, Germany, 2Van Drunen Farms, FutureCeuticals Inc., United States Research of last two decades described oxidative stress as key factor in development and progression of different nation disorders as myocardial infarct, diabetes etc. Formation of reactive oxygen species (ROS), if not appropriately counteracted, leads to oxidative stress and cellular damage. the objective of this pilot study was to investigate effects of in vivo formation of ROS during moderate exercise training as well as during supplementation of mineral and antioxidant formulation elevATPTM or in combination of both.
6
Healthy volunteers used moderate exercised for 20 min or ingested single dose of elevATPTM or combination of both to simulate different level of ROS generation. Employed Vitality & Physical Performance test reflecting metabolic and respiratory activity of cells and production of ROS in vivo. the test was performed in capillary blood using EPR spectrometer E-SCAN under resting conditions and after short-term exercise challenge with or without elevATPTM supplementation. to the same extent we observed changes of cardiac hemodynamic parameters and other metabolic as well as inflammatory blood parameters. In this pilot study we found that moderate exercise as well as exercise challenge inducing formation of ROS up to 25% from resting value. in opposite to that we found up to 20 % decrease in the production of ROS in human volunteers after supplementation of single dose of elevATPTM formulation. in the same time we observed progressive increase in respiratory and metabolic activity of blood cells after moderate exercise and elevATPTM supplementation. Finally we found that extent of compensatory mechanisms were potentiated after combination of moderate exercise with supplementation of elevATPTM formulation. Results of this pilot study pointing on potential discovery of novel mechanism in regulation/adaptation of human body to the shortterm elevation of intracellular generation of ROS! This ongoing research continues to offer new perspectives on the creation of new formulations for the future supplementation to groups exposed to oxidative stress such as excessive exercise, metabolic and cardiovascular disorders, smoking, mental stress.
Design and methods: Ninety exclusively breastfed infants were randomized to 1 of 3 feeding groups: iron fortified cereal, iron fortified cereal with fruit or meat. Urine and stool samples were collected while exclusively breastfeeding and before introduction of solid foods (4-5.5 months) and 3 weeks after introduction of these foods to assess the following markers: urinary F2 isoprostanes (LC-MS-MS), urinary 8OH-deoxy guanosine (ELISA), fecal calprotectin (ELISA), reactive oxygen species (ROS-HPLC) and non-heme iron generation (Colorimetric) in the stool, and the fecal microbiome (16S RNA gene pyrosequencing). Results: preliminary results are presented for F2-isoprostanes and ROS generation: neither ROS generation (baseline: 0.031±0.019; after feeds 0.031 ±0.015 RU; n=12) nor urinary isoprostanes (baseline: 10.1±7.2; after feeds 12.8 ±7.6 ng/mg
6)5%0