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Ontogenetic expression of corpus striatum-specific adenylyl cyclase in the rat striatum
SlOl
Localization of mRNA for ~110 isoforms of phosphatidlinositol 3-kinase (PI3K) in the rat central nervous system. Yasushi Ito,Kaoru Goto,and Hisatake Kondo,Department of Anatomy .Tohoku University School of Medicine,Sendai(Japan) In order to gain clues to the role of PI3K in the central nervous system, we examined the expresson of mRNAs for the regulatry subunit 903
isoforms (PllOa
and ~1108) in rat brain by in situ hybridization
The mRNAs for pllOa
histochemistry.
and pllOfl showed a parallel expression pattern in rat brain during pre- and postnatal development.
High expression levels for both ~110 isoforms were found in the mantle and ventricular zones throughout spinal cord on the embryonic day 15 (ElS) deneased slightly.
moderate expression
the
. On El8 and E20, the expression levels remained high in the brain, whereas those in the spinal cord
On postnatal day 1 (Pl) aud P7, the expression
cerebellar cortices, hippocampus,
the entire neuroaxis including
and striatum,
was seen at moderate levels in neurons
whereas the remainder portions
for the mRNAs was maintained
of the olfactory bulb, cerebral and
of the brain expressed the mRNAs weakly to faintly.
in the cerebellar Purkinje and granule cells, whereas substantial,
though
On P21,
low, levels of
expression were detected in the hippocampal and olfactory neuronal layers.
The present finding suggests that PI3K may contribute to signal transduction not only in the neurogenesis and early neurodiffereniiation
but also
in some specific roles of certain mature neurons.
ONTOGENETIC EXPRESSION OF CORPUS STRIATUM-SPECIFIC ADENYLYL CYCLASE IN THE RAT STRIATUM. HIROYUKI SAKAGAMI, YOSHIHIRO SAWAMURA, AND HISATAKE KONDO. Department of Anatomy, Tohoku University School of Medicine, Seiryo-machi 2-1, Aobaku, Sendai 980, Japan. 904
In the striatum, dopamine Dl receptor is known to stimulate adenylyl cyclase via GTP-binding proteins.We have examined the ontogenetic expression of the corpus striatum-specific adenylyl cyclase (ACst) mRNA in the rat striatum by in situ hybridization histochemistry. On E18, weak expression signals for ACst mRNA were first visible in the ventrolateral margin of the caudate putamen. On E20 to P3, ACst mRNA was preferentially localized in ventrolateral margin and striosomes with the patchy pattern. After P7, this patchy pattern evolved to the homogeneous distribution throughout the striatum. By densitomeric analysis, the expression levels of ACst mRNA in the striatum streadily increased after birth, and peaked by P14. Thereafter, the expression levels of ACst mRNA gradually decreased. These ontogenetic expression is in well agreement with the previous ligand-binding studies of the dopamine Dl receptor, suggesting that ACst is involved in the maturation and differentiation of the striatal neurons through the dopamine Dl receptor.
905
AN AlTEMPT TO ISOLATE PURIFIED SYNAPTIC VESICLES FROM JUNKO MATSUURA1. m IDE2, HII(OSHITORUMRU3 c @&A jOSHIDA1, YUTAKAKIRINO’AND m
Neurobiol. L Sch. of HuaanSci., WasedaUniversity, Tokorozawa359, Faculty of Pharmaceutical Science, m sl?, ‘Faculty of Pharmaceutical Science, University of Tokyo, But&-o-ku,Tokyo 113, Japan. Isolation of neurotransmitter membrane fusion. and bovine brain, vesicles elevated
synaptic vesicle release, because Data accumulated independently. because highly,
RAT
BRAIN
YOSHIOKA1, 1Dept. of Mol. University, Hipashi-ku, Fukuoka
is
very important to study molecular mechanism of to decide molecular machinery for is easy so far were obtained mainly using electric organ Recently needs to prepare rat brain synaptic most of the physiological and biochemical works it
in the field of neuroscience have been performed using rat brain. We have prepared synaptic vesicle fractions from rat brain and from electric organ of Japanese were examined by electron electric ray. Purity of these two types of vesicle microscopy and the activity of the proton pumping vacuolar ATPase (V-ATPase). After the purification procedure, vesicle associated proteins were analyzed with various kinds of antibodies for synaptophysin and SNAP-25 using western blot. Interesting1Y. the ATP peak fraction obtained from electric organ recognized with antisynaptophysin antibody, while that from rat brain did not, although whole protein from rat brain has a component which react with the antibody (= 38 KDa). These results suggest that neurotransmitter from rat brain is not co-existent with ATP.
Localization of mRNA for ~110 isoforms of phosphatidlinositol 3-kinase (PI3K) in the rat central nervous system. Yasushi Ito,Kaoru Goto,and Hisatake Kondo,Department of Anatomy .Tohoku University School of Medicine,Sendai(Japan) In order to gain clues to the role of PI3K in the central nervous system, we examined the expresson of mRNAs for the regulatry subunit 903
isoforms (PllOa
and ~1108) in rat brain by in situ hybridization
The mRNAs for pllOa
histochemistry.
and pllOfl showed a parallel expression pattern in rat brain during pre- and postnatal development.
High expression levels for both ~110 isoforms were found in the mantle and ventricular zones throughout spinal cord on the embryonic day 15 (ElS) deneased slightly.
moderate expression
the
. On El8 and E20, the expression levels remained high in the brain, whereas those in the spinal cord
On postnatal day 1 (Pl) aud P7, the expression
cerebellar cortices, hippocampus,
the entire neuroaxis including
and striatum,
was seen at moderate levels in neurons
whereas the remainder portions
for the mRNAs was maintained
of the olfactory bulb, cerebral and
of the brain expressed the mRNAs weakly to faintly.
in the cerebellar Purkinje and granule cells, whereas substantial,
though
On P21,
low, levels of
expression were detected in the hippocampal and olfactory neuronal layers.
The present finding suggests that PI3K may contribute to signal transduction not only in the neurogenesis and early neurodiffereniiation
but also
in some specific roles of certain mature neurons.
ONTOGENETIC EXPRESSION OF CORPUS STRIATUM-SPECIFIC ADENYLYL CYCLASE IN THE RAT STRIATUM. HIROYUKI SAKAGAMI, YOSHIHIRO SAWAMURA, AND HISATAKE KONDO. Department of Anatomy, Tohoku University School of Medicine, Seiryo-machi 2-1, Aobaku, Sendai 980, Japan. 904
In the striatum, dopamine Dl receptor is known to stimulate adenylyl cyclase via GTP-binding proteins.We have examined the ontogenetic expression of the corpus striatum-specific adenylyl cyclase (ACst) mRNA in the rat striatum by in situ hybridization histochemistry. On E18, weak expression signals for ACst mRNA were first visible in the ventrolateral margin of the caudate putamen. On E20 to P3, ACst mRNA was preferentially localized in ventrolateral margin and striosomes with the patchy pattern. After P7, this patchy pattern evolved to the homogeneous distribution throughout the striatum. By densitomeric analysis, the expression levels of ACst mRNA in the striatum streadily increased after birth, and peaked by P14. Thereafter, the expression levels of ACst mRNA gradually decreased. These ontogenetic expression is in well agreement with the previous ligand-binding studies of the dopamine Dl receptor, suggesting that ACst is involved in the maturation and differentiation of the striatal neurons through the dopamine Dl receptor.
905
AN AlTEMPT TO ISOLATE PURIFIED SYNAPTIC VESICLES FROM JUNKO MATSUURA1. m IDE2, HII(OSHITORUMRU3 c @&A jOSHIDA1, YUTAKAKIRINO’AND m
Neurobiol. L Sch. of HuaanSci., WasedaUniversity, Tokorozawa359, Faculty of Pharmaceutical Science, m sl?, ‘Faculty of Pharmaceutical Science, University of Tokyo, But&-o-ku,Tokyo 113, Japan. Isolation of neurotransmitter membrane fusion. and bovine brain, vesicles elevated
synaptic vesicle release, because Data accumulated independently. because highly,
RAT
BRAIN
YOSHIOKA1, 1Dept. of Mol. University, Hipashi-ku, Fukuoka
is
very important to study molecular mechanism of to decide molecular machinery for is easy so far were obtained mainly using electric organ Recently needs to prepare rat brain synaptic most of the physiological and biochemical works it
in the field of neuroscience have been performed using rat brain. We have prepared synaptic vesicle fractions from rat brain and from electric organ of Japanese were examined by electron electric ray. Purity of these two types of vesicle microscopy and the activity of the proton pumping vacuolar ATPase (V-ATPase). After the purification procedure, vesicle associated proteins were analyzed with various kinds of antibodies for synaptophysin and SNAP-25 using western blot. Interesting1Y. the ATP peak fraction obtained from electric organ recognized with antisynaptophysin antibody, while that from rat brain did not, although whole protein from rat brain has a component which react with the antibody (= 38 KDa). These results suggest that neurotransmitter from rat brain is not co-existent with ATP.