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OP022 EFFECTS OF POTASSIUMBICARBONATE ON BONE RESORPTION DURING HIGH PROTEIN INTAKE IN BED REST (MEP STUDY)
Oral communications 4: Nutrition, cancer and chronic diseases
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an index of net protein loss, was significantly (p < 0.05) decreased by hypoxia. Exposure to hypoxia in bed rest did not change protein turnover and phenylalanine hydroxylation. Conclusion: Hypoxia per se, at normal level of physical activity, decreases whole body protein turnover leading to a net anticatabolic effect. These effects of hypoxia on protein kinetics were completely blunted by physical inactivity.
Oral communications 4: Nutrition, cancer and chronic diseases
Disclosure of Interest: None Declared
M. Heer1,2 , J. Buehlmeier3 , S.M. Smith4 , N. B¨ acker5 , 3 1 P. Frings-Meuthen . Nutrition and Food Sciences, University of Bonn, Bonn, 2 Nutritional Sciences, Profil Neuss, Neuss, 3 Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; 4 Nutritional Biochemistry Laboratory, NASA, Houston, United States; 5 Nutrition and Food Sciences, University of Bonn, Neuss, Germany
OP021 TARGETED METABOLIC PROFILES IN RESPONSE TO HIGH-FRUCTOSE DIETS IN LEAN AND OBESE WOMEN A. Damms Machado1 , I. Bißbort1 , M. Basrai1 , A.A. Roscher2 , S.C. Bischoff1 . 1 Dept. of Nutritional Medicine, University of Hohenheim, Stuttgart, 2 Research Center, Dr. von Hauner Children’s Hospital, Ludwig-Maximilians-Universit¨ at M¨ unchen, Munich, Germany Rationale: Evidence suggests a progressive association between high-fructose corn syrup (HFCS) consumption, obesity and the metabolic syndrome. Hepatic metabolism of fructose is hypothesized to promote de novo lipogenesis, intrahepatic lipid accumulation, and formation of reactive oxygen-species. This study aims to assess effects of high-fructose intake on metabolic profiles. Methods: A crossover study including obese (n = 6) and lean (n = 6) women was performed comprising the following 1 week-intervention phases: A low-fructose diet (10 g/d), B high-fructose diet (100 g/d) (natural sources: fruits), C lowfructose diet (10 g/d), D high-fructose diet (100 g/d) (HFCS). The metabolite set included acylcarnitines (AC), amino acids, biogenic amines, hexoses, sphingo- and phospholipids (PC). Data were subjected to principal component analysis (PCA) to observe general clustering, as well as OPLS-DA models to identify key metabolites. Results: Both high-fructose diets showed similar metabolic shifts. We observed a distinct decrease of long-chain AC in response to high-fructose diets (B p = 0.005, D p = 0.091) which was reversible after the low-fructose week (C p = 0.01) and more pronounced in lean subjects. HFCS consumption lead to a significant increase in the sum of lysoPC (113.9±23.2mM/l to 153.8±30.9mM/l, p < 0.01) only in obese subjects. PCA revealed a clear separation of metabolic profiles in obese compared to lean individuals independent of diet. The Methionine-sulfoxide/Methionine ratio was significantly higher in obese subjects (0.103±0.017 vs 0.02±0.003, p < 0.001). Conclusion: A short-time high-fructose-intervention induced metabolic shifts resulting in an inhibition of mitochondrial beta-oxidation of long-chain fatty acids independent of fructose source. The increase in lysoPC can be explained by an increased lipid peroxidation. Moreover, obese subjects showed higher levels of oxidative stress as assessed by the Met-SO/Met ratio. Disclosure of Interest: None Declared
OP022 EFFECTS OF POTASSIUMBICARBONATE ON BONE RESORPTION DURING HIGH PROTEIN INTAKE IN BED REST (MEP STUDY)
Rationale: Protein supplementation in bed rest may increase protein synthesis, but it also increases bone resorption, likely mediated by low-grade metabolic acidosis. The hypothesis of our trial was that providing KHCO3 with whey protein (WP) will prevent the increase in bone resorption that results from WP alone. Methods: We conducted a crossover study with 9 healthy male subjects (age 31±6 y, body mass [BM] 77±7 kg). In both study phases, following a 7-d ambulatory period, subjects participated in 21-d of 6º head-down tilt bed rest (HDBR). Diet and environmental conditions were standardized across phases. In the control phase, subjects received no supplements. In the intervention phase, they received 6 doses per day of 0.6 g WP/kg BM together with 15 mmol KHCO3. WP was added to the nominal dietary protein, isocalorically (i.e., fat and CHO reduced). Results: HDBR led to an increase in excretion of bone resorption markers C- (CTX) and N-telopeptide (NTX) by 85±28% (p < 0.001) and 35±19% (p < 0.001), respectively. WP/KHCO3 prevented an additional rise of these markers. The bone formation marker procollagen-I-N-terminal propeptide was increased (p = 0.02) with WP/KHCO3, while bone alkaline phosphatase was unchanged. Conclusion: We conclude that increased bone resorption from high protein intake during bed rest can be mitigated by KHCO3 . Disclosure of Interest: None Declared
OP023 LONG TERM CELLULAR AND PLASMA UPTAKE OF PARENTERAL OMEGA-3 FATTY ACIDS IN PATIENTS WITH ADVANCED PANCREATIC CANCER A. Arshad1 , J. Isherwood1 , C. Mann1 , J. Cooke1 , P. Calder2 , W. Steward3 , M. Metcalfe1 , A. Dennison1 . 1 Hepatobiliary and Pancreatic Surgery, University Hospitals of Leicester, Leicester, 2 Nutritional Immunology, University of Southampon, Southampton, 3 Medical Oncology, University Hospitals of Leicester, Leicester, United Kingdom Rationale: Cellular and plasma phospholipid uptake of parenteral Omega-3 rich fatty acid (n-3FA) preparations have been studied in short term regimens, with some long term uptake data available in oral preparations. No study to date has examined uptake with regular parenteral n-3FA administration over periods of up to 6 months. Investigation
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an index of net protein loss, was significantly (p < 0.05) decreased by hypoxia. Exposure to hypoxia in bed rest did not change protein turnover and phenylalanine hydroxylation. Conclusion: Hypoxia per se, at normal level of physical activity, decreases whole body protein turnover leading to a net anticatabolic effect. These effects of hypoxia on protein kinetics were completely blunted by physical inactivity.
Oral communications 4: Nutrition, cancer and chronic diseases
Disclosure of Interest: None Declared
M. Heer1,2 , J. Buehlmeier3 , S.M. Smith4 , N. B¨ acker5 , 3 1 P. Frings-Meuthen . Nutrition and Food Sciences, University of Bonn, Bonn, 2 Nutritional Sciences, Profil Neuss, Neuss, 3 Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; 4 Nutritional Biochemistry Laboratory, NASA, Houston, United States; 5 Nutrition and Food Sciences, University of Bonn, Neuss, Germany
OP021 TARGETED METABOLIC PROFILES IN RESPONSE TO HIGH-FRUCTOSE DIETS IN LEAN AND OBESE WOMEN A. Damms Machado1 , I. Bißbort1 , M. Basrai1 , A.A. Roscher2 , S.C. Bischoff1 . 1 Dept. of Nutritional Medicine, University of Hohenheim, Stuttgart, 2 Research Center, Dr. von Hauner Children’s Hospital, Ludwig-Maximilians-Universit¨ at M¨ unchen, Munich, Germany Rationale: Evidence suggests a progressive association between high-fructose corn syrup (HFCS) consumption, obesity and the metabolic syndrome. Hepatic metabolism of fructose is hypothesized to promote de novo lipogenesis, intrahepatic lipid accumulation, and formation of reactive oxygen-species. This study aims to assess effects of high-fructose intake on metabolic profiles. Methods: A crossover study including obese (n = 6) and lean (n = 6) women was performed comprising the following 1 week-intervention phases: A low-fructose diet (10 g/d), B high-fructose diet (100 g/d) (natural sources: fruits), C lowfructose diet (10 g/d), D high-fructose diet (100 g/d) (HFCS). The metabolite set included acylcarnitines (AC), amino acids, biogenic amines, hexoses, sphingo- and phospholipids (PC). Data were subjected to principal component analysis (PCA) to observe general clustering, as well as OPLS-DA models to identify key metabolites. Results: Both high-fructose diets showed similar metabolic shifts. We observed a distinct decrease of long-chain AC in response to high-fructose diets (B p = 0.005, D p = 0.091) which was reversible after the low-fructose week (C p = 0.01) and more pronounced in lean subjects. HFCS consumption lead to a significant increase in the sum of lysoPC (113.9±23.2mM/l to 153.8±30.9mM/l, p < 0.01) only in obese subjects. PCA revealed a clear separation of metabolic profiles in obese compared to lean individuals independent of diet. The Methionine-sulfoxide/Methionine ratio was significantly higher in obese subjects (0.103±0.017 vs 0.02±0.003, p < 0.001). Conclusion: A short-time high-fructose-intervention induced metabolic shifts resulting in an inhibition of mitochondrial beta-oxidation of long-chain fatty acids independent of fructose source. The increase in lysoPC can be explained by an increased lipid peroxidation. Moreover, obese subjects showed higher levels of oxidative stress as assessed by the Met-SO/Met ratio. Disclosure of Interest: None Declared
OP022 EFFECTS OF POTASSIUMBICARBONATE ON BONE RESORPTION DURING HIGH PROTEIN INTAKE IN BED REST (MEP STUDY)
Rationale: Protein supplementation in bed rest may increase protein synthesis, but it also increases bone resorption, likely mediated by low-grade metabolic acidosis. The hypothesis of our trial was that providing KHCO3 with whey protein (WP) will prevent the increase in bone resorption that results from WP alone. Methods: We conducted a crossover study with 9 healthy male subjects (age 31±6 y, body mass [BM] 77±7 kg). In both study phases, following a 7-d ambulatory period, subjects participated in 21-d of 6º head-down tilt bed rest (HDBR). Diet and environmental conditions were standardized across phases. In the control phase, subjects received no supplements. In the intervention phase, they received 6 doses per day of 0.6 g WP/kg BM together with 15 mmol KHCO3. WP was added to the nominal dietary protein, isocalorically (i.e., fat and CHO reduced). Results: HDBR led to an increase in excretion of bone resorption markers C- (CTX) and N-telopeptide (NTX) by 85±28% (p < 0.001) and 35±19% (p < 0.001), respectively. WP/KHCO3 prevented an additional rise of these markers. The bone formation marker procollagen-I-N-terminal propeptide was increased (p = 0.02) with WP/KHCO3, while bone alkaline phosphatase was unchanged. Conclusion: We conclude that increased bone resorption from high protein intake during bed rest can be mitigated by KHCO3 . Disclosure of Interest: None Declared
OP023 LONG TERM CELLULAR AND PLASMA UPTAKE OF PARENTERAL OMEGA-3 FATTY ACIDS IN PATIENTS WITH ADVANCED PANCREATIC CANCER A. Arshad1 , J. Isherwood1 , C. Mann1 , J. Cooke1 , P. Calder2 , W. Steward3 , M. Metcalfe1 , A. Dennison1 . 1 Hepatobiliary and Pancreatic Surgery, University Hospitals of Leicester, Leicester, 2 Nutritional Immunology, University of Southampon, Southampton, 3 Medical Oncology, University Hospitals of Leicester, Leicester, United Kingdom Rationale: Cellular and plasma phospholipid uptake of parenteral Omega-3 rich fatty acid (n-3FA) preparations have been studied in short term regimens, with some long term uptake data available in oral preparations. No study to date has examined uptake with regular parenteral n-3FA administration over periods of up to 6 months. Investigation