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OP36 Evaluation of nutritional effect of quail eggs on blood glucose, lipid profile and oxidative stress parameters in diabetic induced rats
DIABETES RESEARCH A N D CLINICAL PRACTICE
une fraction importante et croissante des personnes âgées et des diabétiques. La principale cause de décès chez les diabétiques est représentée par les maladies cardiovasculaires. Le but de notre travail était d’illustrer les aspects cliniques des complications dégénératives du diabétique âgé. Méthodes : Il s’agissait d’une étude transversale rétrospective descriptive qui s’est déroulée sur une période de 2 mois (février à mars 2013). Elle a porté sur les diabétiques âgés de 60 ans et plus, suivis au Centre Antidiabétique d’Abidjan. Résultats : Au total, 157 patients ont été inclus dans l’étude. L’âge moyen était de 66,8±7,2 ans avec des extrêmes de 60 et 91 ans. On notait une prédominance féminine dans 55 % des cas. En moyenne, la durée d’évolution du diabète était estimée à 9,6±7,9 ans. La prévalence de l’hypertension artérielle et des complications dégénératives du diabète était respectivement de 69 % et 95,5 %. La microangiopathie présente chez 139 patients soit 92,7 % des cas et était dominée par la polyneuropathie (73,4 %) suivi de la dysfonction érectile (24,5 %). La rétinopathie et la néphropathie diabétique étaient notées respectivement dans 20,1 % et 15,1 % des cas. Les complications macrovasculaires étaient présentes chez 19,3 % des patients et a intéressé les trois principaux lits vasculaires avec comme expression clinique les accidents vasculaires cérébraux (41,4 %), les coronaropathies (37,9 %) et l’artériopathie oblitérante des membres inférieurs (34,5 %). Conclusion : La prévention des complications dégénératives notamment des macroangiopathies chez le sujet âgé diabétique reste une priorité.
OP36 Evaluation of nutritional effect of quail eggs on blood glucose, lipid profile and oxidative stress parameters in diabetic induced rats E. Lontchi Yimagou a , A. Tanya b , C. Tchankou a , J. Ngondi a , J. Oben a . a Laboratory of Nutrition and Nutritionnal Biochemistry/University of Yaounde I; b Faculty of Medicine and Biomedical Sciences, Department of Public Health, University of Yaounde I, Yaounde, Cameroon Introduction: Quail eggs are a new food item that has recently been introduced into the diet of some Cameroonians. These small colourful eggs are being advertised in some shops in the cities with many unfounded health claims. One of these claims is that quail eggs can reduce blood glucose levels in diabetics. It was therefore necessary to evaluate the effect of quail eggs on blood glucose, lipid profile and oxidative stress parameters in diabetic induced rats. Methods: Twenty Wistar rats weighing on average 250 g were divided into four groups of five each. Group 1 consisted of rats with normal blood glucose and the other 3 groups (2, 3 and 4) consisted of diabetic induced rats by intravenous injection of streptozotocin. For 16 days, groups 1 and 2 received distilled water, groups 3 and 4 received quail and chicken eggs respectively with gastro oesophageal probe at a dose of 1 mL/200 g body weight. The experiment lasted 17 days. Fasting blood glucose levels were determined in all groups on the 1st, 7th, 14th and 17th days after induction of diabetes. On the 17th day, the fasting rats were sacrificed; blood and liver were collected for biochemical analysis. Results: In 17 days, the consumption of quail and chicken eggs had no effect on blood glucose levels of diabetic rats. Total cholesterol level were higher in groups 3 (75.59 mg/dL) and 4 (59.41 mg/dL) compared to group 2 (55.67 mg/dL) although this difference was non significant. Also triglyceride levels were significantly higher (P<0.05) in group 3 (106.52 mg/dL) and 4 (109.65 mg/dL) compared to group 2 (65.82 mg/dL). Quail eggs had no effect on oxidative stress parameters (Malondialdehyde, hydroperoxides and catalase). Conclusion: The consumption of quail eggs by diabetic
103S (2014) S 1–S61
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patients would have no effect on blood glucose, oxidative stress parameters and may have a negative effect on lipid profile.
OP37 Guinean antidiabetic plants and their potential anticancer activity E.S. Baldé a , N.M. Baldé b , A. Diallo a , M.S. Traoré a , V. Mégalizzi c , A.M. Baldé a . a Centre de Recherche et de Valorisation des Plantes Médicinales, Dubréka, Guinée, Département de Pharmacie, Faculté de Médecine-Pharmacie-Odontostomatologie, Université de Conakry, Guinée; b Service d’endocrinologie, CHU Donka Conakry Guinée, Département de Médecine, FMPOS Université Gamal Abdel Nasser, Conakry, Guinée; c Laboratoire de toxicologie, Institut de Pharmacie, ULB Bruxelles Belgique Introduction: Previously studies have reported the link between diabetes and various cancers. Since plant species have been described to play an important role in diabetes management. The aim of this study was to explore the anticancer activity of Guinean medicinal plants widely used in the management of diabetes. Methods: Five plants (Anacardium occidentale, Alchornea cordifolia, Bridellia ferruginea, Cassia siberiana, Piliostigma thonningii) widely used in the Guinean traditional medicine were selected and two extracts (polar and apolar) were prepared for each plant. The in vitro antigrowth activity using the colorimetric 3-[4,5-dimethylthiazol-2yl]-diphenyl tetrazolium bromide assay (MTT) were evaluated for 4 cancer cells lines (Hs683, U373, MCF7, B16F10). Results: All the apolar extract displayed a significant growth inhibitory activity against the 4 cancer cell lines studied. From while the polar extracts only that of Bridellia ferruginea displayed an activity which was strong. The cytotoxicity was moderate (10 μg/ml < IC50 < 50 μg/ml) for Anacardium occidentale, Alchornea cordifolia, Cassia siberiana good (1 μg/ml < IC50 < 5 μg/ml) for Piliostigma thonningii and strong (0,1 μg/ml < IC50 < 0,5 μg/ml) for Bridellia ferruginea. Conclusion: These plants may be playing an important role in cancer chemoprevention. Previous studies reported antioxidant activity for Piliostigma thonningii and the inhibition of the NF-κB and MAPK, two signaling pathway implicated in the cancer cell proliferation and migration by Bridellia ferruginea, Anacardium occidentale. The bio-guided fractionation will be plan for the research of the compound with a potential anticancer activity.
OP39 Evaluation of the hypoglycemic, hypolipidemic and anti alpha amylase effects of extracts of the twigs and fruits of Ficus ovata Vahl (Moraceae) M. Fouondo, J. Oben, C. Fokunang. University of Yaoundé I Introduction: The phytochemical component of many medicinal plants have most often been linked to the modulation of biomarkers associated to type 2 diabetes. Previous studies done the hydroethanolic fruits and hydroethanolic twigs of Ficus ovata, revealed the presence of groups of bioactive compounds such as alkaloids, glycosides, saponins and polyphenols (flavanoids, tannins and phenols). These extracts also showed antioxidant scavenging effects which is an important component in the management of type 2 diabetes. This study was aimed at evaluating the in vitro anti alpha amylase, antihyperglycemic and antihyperlipidemic effects of Ficus ovata. Methods: The hydroethanolic extracts of the fruits and twigs were used for the in vitro anti alpha amylase assay, a postprandial blood glucose test (OGTT) on normal rats. Moreover, the preventive effects of the extracts against some biomarkers of diabetes (lipid profile, endothelia dysfunction, hepatic and renal
une fraction importante et croissante des personnes âgées et des diabétiques. La principale cause de décès chez les diabétiques est représentée par les maladies cardiovasculaires. Le but de notre travail était d’illustrer les aspects cliniques des complications dégénératives du diabétique âgé. Méthodes : Il s’agissait d’une étude transversale rétrospective descriptive qui s’est déroulée sur une période de 2 mois (février à mars 2013). Elle a porté sur les diabétiques âgés de 60 ans et plus, suivis au Centre Antidiabétique d’Abidjan. Résultats : Au total, 157 patients ont été inclus dans l’étude. L’âge moyen était de 66,8±7,2 ans avec des extrêmes de 60 et 91 ans. On notait une prédominance féminine dans 55 % des cas. En moyenne, la durée d’évolution du diabète était estimée à 9,6±7,9 ans. La prévalence de l’hypertension artérielle et des complications dégénératives du diabète était respectivement de 69 % et 95,5 %. La microangiopathie présente chez 139 patients soit 92,7 % des cas et était dominée par la polyneuropathie (73,4 %) suivi de la dysfonction érectile (24,5 %). La rétinopathie et la néphropathie diabétique étaient notées respectivement dans 20,1 % et 15,1 % des cas. Les complications macrovasculaires étaient présentes chez 19,3 % des patients et a intéressé les trois principaux lits vasculaires avec comme expression clinique les accidents vasculaires cérébraux (41,4 %), les coronaropathies (37,9 %) et l’artériopathie oblitérante des membres inférieurs (34,5 %). Conclusion : La prévention des complications dégénératives notamment des macroangiopathies chez le sujet âgé diabétique reste une priorité.
OP36 Evaluation of nutritional effect of quail eggs on blood glucose, lipid profile and oxidative stress parameters in diabetic induced rats E. Lontchi Yimagou a , A. Tanya b , C. Tchankou a , J. Ngondi a , J. Oben a . a Laboratory of Nutrition and Nutritionnal Biochemistry/University of Yaounde I; b Faculty of Medicine and Biomedical Sciences, Department of Public Health, University of Yaounde I, Yaounde, Cameroon Introduction: Quail eggs are a new food item that has recently been introduced into the diet of some Cameroonians. These small colourful eggs are being advertised in some shops in the cities with many unfounded health claims. One of these claims is that quail eggs can reduce blood glucose levels in diabetics. It was therefore necessary to evaluate the effect of quail eggs on blood glucose, lipid profile and oxidative stress parameters in diabetic induced rats. Methods: Twenty Wistar rats weighing on average 250 g were divided into four groups of five each. Group 1 consisted of rats with normal blood glucose and the other 3 groups (2, 3 and 4) consisted of diabetic induced rats by intravenous injection of streptozotocin. For 16 days, groups 1 and 2 received distilled water, groups 3 and 4 received quail and chicken eggs respectively with gastro oesophageal probe at a dose of 1 mL/200 g body weight. The experiment lasted 17 days. Fasting blood glucose levels were determined in all groups on the 1st, 7th, 14th and 17th days after induction of diabetes. On the 17th day, the fasting rats were sacrificed; blood and liver were collected for biochemical analysis. Results: In 17 days, the consumption of quail and chicken eggs had no effect on blood glucose levels of diabetic rats. Total cholesterol level were higher in groups 3 (75.59 mg/dL) and 4 (59.41 mg/dL) compared to group 2 (55.67 mg/dL) although this difference was non significant. Also triglyceride levels were significantly higher (P<0.05) in group 3 (106.52 mg/dL) and 4 (109.65 mg/dL) compared to group 2 (65.82 mg/dL). Quail eggs had no effect on oxidative stress parameters (Malondialdehyde, hydroperoxides and catalase). Conclusion: The consumption of quail eggs by diabetic
103S (2014) S 1–S61
S19
patients would have no effect on blood glucose, oxidative stress parameters and may have a negative effect on lipid profile.
OP37 Guinean antidiabetic plants and their potential anticancer activity E.S. Baldé a , N.M. Baldé b , A. Diallo a , M.S. Traoré a , V. Mégalizzi c , A.M. Baldé a . a Centre de Recherche et de Valorisation des Plantes Médicinales, Dubréka, Guinée, Département de Pharmacie, Faculté de Médecine-Pharmacie-Odontostomatologie, Université de Conakry, Guinée; b Service d’endocrinologie, CHU Donka Conakry Guinée, Département de Médecine, FMPOS Université Gamal Abdel Nasser, Conakry, Guinée; c Laboratoire de toxicologie, Institut de Pharmacie, ULB Bruxelles Belgique Introduction: Previously studies have reported the link between diabetes and various cancers. Since plant species have been described to play an important role in diabetes management. The aim of this study was to explore the anticancer activity of Guinean medicinal plants widely used in the management of diabetes. Methods: Five plants (Anacardium occidentale, Alchornea cordifolia, Bridellia ferruginea, Cassia siberiana, Piliostigma thonningii) widely used in the Guinean traditional medicine were selected and two extracts (polar and apolar) were prepared for each plant. The in vitro antigrowth activity using the colorimetric 3-[4,5-dimethylthiazol-2yl]-diphenyl tetrazolium bromide assay (MTT) were evaluated for 4 cancer cells lines (Hs683, U373, MCF7, B16F10). Results: All the apolar extract displayed a significant growth inhibitory activity against the 4 cancer cell lines studied. From while the polar extracts only that of Bridellia ferruginea displayed an activity which was strong. The cytotoxicity was moderate (10 μg/ml < IC50 < 50 μg/ml) for Anacardium occidentale, Alchornea cordifolia, Cassia siberiana good (1 μg/ml < IC50 < 5 μg/ml) for Piliostigma thonningii and strong (0,1 μg/ml < IC50 < 0,5 μg/ml) for Bridellia ferruginea. Conclusion: These plants may be playing an important role in cancer chemoprevention. Previous studies reported antioxidant activity for Piliostigma thonningii and the inhibition of the NF-κB and MAPK, two signaling pathway implicated in the cancer cell proliferation and migration by Bridellia ferruginea, Anacardium occidentale. The bio-guided fractionation will be plan for the research of the compound with a potential anticancer activity.
OP39 Evaluation of the hypoglycemic, hypolipidemic and anti alpha amylase effects of extracts of the twigs and fruits of Ficus ovata Vahl (Moraceae) M. Fouondo, J. Oben, C. Fokunang. University of Yaoundé I Introduction: The phytochemical component of many medicinal plants have most often been linked to the modulation of biomarkers associated to type 2 diabetes. Previous studies done the hydroethanolic fruits and hydroethanolic twigs of Ficus ovata, revealed the presence of groups of bioactive compounds such as alkaloids, glycosides, saponins and polyphenols (flavanoids, tannins and phenols). These extracts also showed antioxidant scavenging effects which is an important component in the management of type 2 diabetes. This study was aimed at evaluating the in vitro anti alpha amylase, antihyperglycemic and antihyperlipidemic effects of Ficus ovata. Methods: The hydroethanolic extracts of the fruits and twigs were used for the in vitro anti alpha amylase assay, a postprandial blood glucose test (OGTT) on normal rats. Moreover, the preventive effects of the extracts against some biomarkers of diabetes (lipid profile, endothelia dysfunction, hepatic and renal