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OP6-6 High frequency of SEN virus infection in thalassemic patients and healthy blood donors in Iran
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Oral presentations, 12th ESCV, Istanbul / Journal of Clinical Virology 46 Suppl. 1 (2009) S1–S14
over time. We assessed the extent to which betaPV infections persist in a community with a high incidence of SCC. Methods: In 1996 and in 2003 samples were collected from 171 participants of the Autralian community-based Nambour Skin Cancer Study. SCC-precursors (actinic keratoses) were scored by skin examinations at baseline and again in 2006. Details of skin type and sun exposure were collected by standard questionnaire. Viral DNA was isolated from plucked eyebrow hairs and analysed the presence of 25 different betaPV-types. Results: Participants’ mean age at baseline was 50, 49% were males. In 1996 a total of 413 beta-PV infections were found in 73% of participants; in 2003 this rose to 490 infections in 85% of participants. Of the total number of infections, 211 (30%) were found to persist. Age was significantly associated with betaPV persistence whereas skin type, occupational sun exposure and sunburn history were not. Persistence of betaPV infection resulted in a significant doubling of the odds of having actinic keratoses on the face in 2007. Conclusions: We showed that around one third of the betaPV infections persisted in the tested population over an 8-year period, especially in the older people. Persistent betaPV infection increased the subsequent risk of actinic keratoses, suggesting that long-term betaPV infections play a role in development of SCC. OP6-6 High frequency of SEN virus infection in thalassemic patients and healthy blood donors in Iran A. Karimi-Rastehkenari *, M. Bouzari. Department of Biology, Faculty of science, University of Isfahan, Isfahan, Iran SEN virus is a bloodborne, circular ssDNA virus and possessing nine different genotypes (A to I). Infection with bloodborne causes is the second important cause of death in thalassemic patients. The aim of this study was to determine the frequency of SENV-D and SENV-H genotypes viremia in thalassemic patients and healthy blood donors in Guilan Province, North of Iran. Nested-PCR was used for identification of the SENV-D and SENV-H genotypes in 120 and 100 sera from healthy blood donors and thalassemic patients, respectively. Serum aminotransferases (ALT and AST) and some of the blood factors were measured. Frequency of SENV-D, SENV (SENV-H or SENV-D) and coinfection (both SENV-D and SENV-H) viremia was significantly higher among thalassemic patients than healthy blood donors. Frequency of SENV-H viremia was significantly higher than SENV-D among healthy individuals. In comparison to SENV-D negative patients, the mean of mean corpuscular hemoglobin (MCH) was significantly higher in SENV-D positive and coinfection cases ( p<0.05). The high rate of coinfection shows that different genotypes of the virus have no negative effects on each other. The high frequency of SENV infection among thalassemic patients suggests that the main route of transmission is blood transfusion. High frequency of infection in healthy blood donors indicates that other routes rather than blood transfusion also are important. In conclusion, high frequency (90.8%) of SENV infection among healthy blood donors can probably suggest that healthy blood donors are partly as a source of SENV transmission to the thalassemic patients and possibly to other community groups. OP6-7 Case series of intra-family cmv transmission: molecular analysis of the transmission cascade and longitudinal CMV specific phospho-and glycoprotein antibody patterns during primary infections K. Hamprecht1 *, X.J. Jiang1 , J.A. Arellano-Galindo1 , K. G¨ohring1 , E. Mikeler1 , G. Jahn1 , A.L. Bissinger2 . 1 Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tuebingen, Tuebingen, Germany, 2 Dep Gastroenterology and Infectious Diseases, University Hospital of Tuebingen, Tuebingen, Germany Young children have been described as a probable source of maternal and congenital CMV infection by family studies and an increased rate of CMV infection has been reported among parents of children attending american daycare centers (Pass et al., 1986, 1987). In this context for Germany no detailed data are available We describe an unusual well documented cascade of intra-family CMV transmission from a 9 months old otherwise healthy girl attending a day care center to her mother, grand-mother and mother-in-law. The mother developed a pneumonia about two months after the onset of the primary infection of the baby requiring hospitalization.
Molecular analysis of the shedded viral strains were performed by genotyping of CMV gB (UL55), gN (UL73) and gO (UL74) genes and sequence alignment of hypervariable gO sequence regions. Longitudinal serological analysis included recombinant immunoblot analysis, and avidity testing. In all cases genotyping revealed an identical CMV strain. Interestingly, both mother and grand mother showed an identical pattern of IgM reactivity (p150, CM2, p65). The mother exhibited IE1 specific IgM reactivity only short after virus transmission. Maternal viruria and viral DNAuria could be quantified for more than 8 months. CMV primary infection of seronegative adults acquired by close contact during baby sitting may have a symptomatic and even severe course. Molecular evidence is given for infant to mother and intrafamilial CMV transmission. Recombinant immunoblotting is an important diagnostic tool to detect the dynamics of IE1-, phospho-and glycoprotein specific CMV IgG and IgM antibody reactivity patterns during seroconversion. OP6-8 Characterization of a new sandfly fever virus isolated during the 2008 sandfly fever epidemic in Turkey 1 , Y. Uyar1 , E. Ozkaya 1 , M. Ertek1 , G. Dobler2 , M. Dilcher3 , ¨ A. Carhan ¸ M. Spiegel3 , F. Hufert3 , M. Weidmann3 . 1 Refik Saydam National Public Health Agency (RSNPHA), Ankara, Turkey, 2 Institute for Microbiology of the Armed Force, M¨unchen, Germany, 3 University Medicine G¨ottingen, Department of Virology, G¨ottingen, Germany Sandfly fever is a viral infection characterized by rapid onset, with a 3- to 4-day course of high fever and severe debility, but low mortality (5). In 2008 summer, Turkey’s Izmir province on the southwest coast, and the inland provinces of Adana reported cases of fever, headache, nausea, and muscle pain after sandfly bites. Samples of the cases were analysed by serological (IFT), virological (VI) and molecular techniques (454 sequencing). After the initial results indicated sandfly fever, a retrospective study concerning cases of fever provisionally termed ‘Mamak fever’ that had occured in the summer of 2007 and 2008 in the Mamak district of Ankara confirmed these cases also to be sandfly fever cases. In IFT a majority of samples (38%) scored positive for SFSV, followed by 13% positive for SFSV/SFCV and 4% positive for SFCV. A real time RT-PCR developed from the initial sequences obtained by generic L-gene PCR, detected 103 −107 molecules/ml in serum samples. Generic Phlebovirus L-gene PCR from patient material, subsequent virus isolation, and 454 sequencing of the isolate, confirmed the serodata by identifying partial SFSV sequences for the S-, M-, and L-segment altogether covering approximately 87% of the whole genome. The new isolate was termed SFTV. Molecular phylogeny of the sequences determined grouped the S-segment sequence of SFTV next to the SFCV isolate sequences from Cyprus with almost 98% homology. The M-segment sequence determined, clearly groups together with the only SFSV sequence available at a homology of 91.1%. The L-sequence described here is the first described for a SFS type virus.
Oral presentations, 12th ESCV, Istanbul / Journal of Clinical Virology 46 Suppl. 1 (2009) S1–S14
over time. We assessed the extent to which betaPV infections persist in a community with a high incidence of SCC. Methods: In 1996 and in 2003 samples were collected from 171 participants of the Autralian community-based Nambour Skin Cancer Study. SCC-precursors (actinic keratoses) were scored by skin examinations at baseline and again in 2006. Details of skin type and sun exposure were collected by standard questionnaire. Viral DNA was isolated from plucked eyebrow hairs and analysed the presence of 25 different betaPV-types. Results: Participants’ mean age at baseline was 50, 49% were males. In 1996 a total of 413 beta-PV infections were found in 73% of participants; in 2003 this rose to 490 infections in 85% of participants. Of the total number of infections, 211 (30%) were found to persist. Age was significantly associated with betaPV persistence whereas skin type, occupational sun exposure and sunburn history were not. Persistence of betaPV infection resulted in a significant doubling of the odds of having actinic keratoses on the face in 2007. Conclusions: We showed that around one third of the betaPV infections persisted in the tested population over an 8-year period, especially in the older people. Persistent betaPV infection increased the subsequent risk of actinic keratoses, suggesting that long-term betaPV infections play a role in development of SCC. OP6-6 High frequency of SEN virus infection in thalassemic patients and healthy blood donors in Iran A. Karimi-Rastehkenari *, M. Bouzari. Department of Biology, Faculty of science, University of Isfahan, Isfahan, Iran SEN virus is a bloodborne, circular ssDNA virus and possessing nine different genotypes (A to I). Infection with bloodborne causes is the second important cause of death in thalassemic patients. The aim of this study was to determine the frequency of SENV-D and SENV-H genotypes viremia in thalassemic patients and healthy blood donors in Guilan Province, North of Iran. Nested-PCR was used for identification of the SENV-D and SENV-H genotypes in 120 and 100 sera from healthy blood donors and thalassemic patients, respectively. Serum aminotransferases (ALT and AST) and some of the blood factors were measured. Frequency of SENV-D, SENV (SENV-H or SENV-D) and coinfection (both SENV-D and SENV-H) viremia was significantly higher among thalassemic patients than healthy blood donors. Frequency of SENV-H viremia was significantly higher than SENV-D among healthy individuals. In comparison to SENV-D negative patients, the mean of mean corpuscular hemoglobin (MCH) was significantly higher in SENV-D positive and coinfection cases ( p<0.05). The high rate of coinfection shows that different genotypes of the virus have no negative effects on each other. The high frequency of SENV infection among thalassemic patients suggests that the main route of transmission is blood transfusion. High frequency of infection in healthy blood donors indicates that other routes rather than blood transfusion also are important. In conclusion, high frequency (90.8%) of SENV infection among healthy blood donors can probably suggest that healthy blood donors are partly as a source of SENV transmission to the thalassemic patients and possibly to other community groups. OP6-7 Case series of intra-family cmv transmission: molecular analysis of the transmission cascade and longitudinal CMV specific phospho-and glycoprotein antibody patterns during primary infections K. Hamprecht1 *, X.J. Jiang1 , J.A. Arellano-Galindo1 , K. G¨ohring1 , E. Mikeler1 , G. Jahn1 , A.L. Bissinger2 . 1 Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital of Tuebingen, Tuebingen, Germany, 2 Dep Gastroenterology and Infectious Diseases, University Hospital of Tuebingen, Tuebingen, Germany Young children have been described as a probable source of maternal and congenital CMV infection by family studies and an increased rate of CMV infection has been reported among parents of children attending american daycare centers (Pass et al., 1986, 1987). In this context for Germany no detailed data are available We describe an unusual well documented cascade of intra-family CMV transmission from a 9 months old otherwise healthy girl attending a day care center to her mother, grand-mother and mother-in-law. The mother developed a pneumonia about two months after the onset of the primary infection of the baby requiring hospitalization.
Molecular analysis of the shedded viral strains were performed by genotyping of CMV gB (UL55), gN (UL73) and gO (UL74) genes and sequence alignment of hypervariable gO sequence regions. Longitudinal serological analysis included recombinant immunoblot analysis, and avidity testing. In all cases genotyping revealed an identical CMV strain. Interestingly, both mother and grand mother showed an identical pattern of IgM reactivity (p150, CM2, p65). The mother exhibited IE1 specific IgM reactivity only short after virus transmission. Maternal viruria and viral DNAuria could be quantified for more than 8 months. CMV primary infection of seronegative adults acquired by close contact during baby sitting may have a symptomatic and even severe course. Molecular evidence is given for infant to mother and intrafamilial CMV transmission. Recombinant immunoblotting is an important diagnostic tool to detect the dynamics of IE1-, phospho-and glycoprotein specific CMV IgG and IgM antibody reactivity patterns during seroconversion. OP6-8 Characterization of a new sandfly fever virus isolated during the 2008 sandfly fever epidemic in Turkey 1 , Y. Uyar1 , E. Ozkaya 1 , M. Ertek1 , G. Dobler2 , M. Dilcher3 , ¨ A. Carhan ¸ M. Spiegel3 , F. Hufert3 , M. Weidmann3 . 1 Refik Saydam National Public Health Agency (RSNPHA), Ankara, Turkey, 2 Institute for Microbiology of the Armed Force, M¨unchen, Germany, 3 University Medicine G¨ottingen, Department of Virology, G¨ottingen, Germany Sandfly fever is a viral infection characterized by rapid onset, with a 3- to 4-day course of high fever and severe debility, but low mortality (5). In 2008 summer, Turkey’s Izmir province on the southwest coast, and the inland provinces of Adana reported cases of fever, headache, nausea, and muscle pain after sandfly bites. Samples of the cases were analysed by serological (IFT), virological (VI) and molecular techniques (454 sequencing). After the initial results indicated sandfly fever, a retrospective study concerning cases of fever provisionally termed ‘Mamak fever’ that had occured in the summer of 2007 and 2008 in the Mamak district of Ankara confirmed these cases also to be sandfly fever cases. In IFT a majority of samples (38%) scored positive for SFSV, followed by 13% positive for SFSV/SFCV and 4% positive for SFCV. A real time RT-PCR developed from the initial sequences obtained by generic L-gene PCR, detected 103 −107 molecules/ml in serum samples. Generic Phlebovirus L-gene PCR from patient material, subsequent virus isolation, and 454 sequencing of the isolate, confirmed the serodata by identifying partial SFSV sequences for the S-, M-, and L-segment altogether covering approximately 87% of the whole genome. The new isolate was termed SFTV. Molecular phylogeny of the sequences determined grouped the S-segment sequence of SFTV next to the SFCV isolate sequences from Cyprus with almost 98% homology. The M-segment sequence determined, clearly groups together with the only SFSV sequence available at a homology of 91.1%. The L-sequence described here is the first described for a SFS type virus.