- Email: [email protected]
Optimisation of growth condition for “Bacillus licheniformis” and “Bacillus subtilis” by using xylooligosaccharides
Abstracts / Journal of Biotechnology 208 (2015) S5–S120
S77
ecological products enter in a large measure in the attention of specialized organisms, and also in the field of fundamental and applicative researches, being a relative new field but of maximum importance to insure population health. The paper contains new contributions to study the market of agricultural and food products on ecological food section, on consumers’ behaviours towards these products (knowledge, ignorance, tendency to consume these products or not, reaction to price etc.) by applying a questionnaire. Processing the data from questionnaires and establishing the requested correlations allow us to create an actual image upon the demand, selling and consumption of ecological products in Timis county.
and the environment. Regarding risk identification, were also used the references of World Health Organization, which has a special program to assist national authorities to identifying the risks associated with genetically modified organisms. Obtaining different genetically modified organisms, involves inserting different genes and different transfer techniques. Because of this, that risk analysis, about the safety of these foods, has analyzed on a case by case basis. Related to this, research showed that not exist until now recorded information showing that there was harmful effects on human health from the consumption of these foods.
http://dx.doi.org/10.1016/j.jbiotec.2015.06.235
Optimisation of growth condition for “Bacillus licheniformis” and “Bacillus subtilis” by using xylooligosaccharides
Research on spectroscopic composition of safflower seeds Carthamus tinctorius L. grown in the conditions of Southeastern Romania Dobrin Aurora 1,∗ , Marin Doru Ioan 1 , Manolache Fulvia Ancut¸a 2 , Ionescu Valentin 2 1 University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania 2 National Research & Development Institute for Food Bioresources – IBA Bucharest, Romania
E-mail address: aur ora [email protected] (D. Aurora). Safflower is a crop with importance for multiple uses: food, pharmaceuticals, cosmetics, paints and varnishes, and animal feed. Research has been carried out in Southeastern Romania (Moara Domneasc˘a Didactic Farm – 44◦ 29 33 N, 26◦ 15 20 E) in order to assess the suitability of cultivation in that region, and to analyze the quality of achenes and oils produced. The safflower cultivars were CW 88 OL, CW 1221 and Zanzibar. The research results show that the safflower adapted well to the soil under the experimental field conditions. Achenes yield was highest in all three varieties for the fertilizer level with N90P60K60, being 1878.33 kg ha−1 in the CW 1221 variety, 2064.32 kg ha−1 in the CW 88 OL variety and 2184.20 kg ha−1 in the Zanzibar variety. Safflower oil was extracted by Soxhlet method. Based on 1HNMR spectroscopy and using systems of chemometric equations, the composition of safflower oils was established on four classes of fatty acids: tri-unsaturated (undetectable for all varieties), di-unsaturated (the highest value was for Zanzibar: 75.05%), monounsaturated (the highest value was for CW 88 OL: 74.54%) and saturated (the highest value was for CW 88 OL: 11.29%). http://dx.doi.org/10.1016/j.jbiotec.2015.06.236 Achieving a risk analysis for products derived from genetically modified organisms, in terms of food safety Magda Gonciarov 1,∗ , Cristin Coman 2 1
Department of Animal Production and Public Health, Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania 2 Cantacuzino Institute, Bucharest, Romania E-mail address: [email protected] (M. Gonciarov). In the experiment realized by the authors of this paper, have been followed the steps of risk analysis, namely the identification, assessment, management and risk communication, in case of genetically modified organisms, in their relation to animal and human health,
http://dx.doi.org/10.1016/j.jbiotec.2015.06.237
Oana Emilia Constantin ∗ , Daniela Istrati, Cristian Teodor Buruiana, Camelia Vizireanu Food Science, Food Engineering, Food Biotechnology Department, Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, Romania E-mail address: [email protected] (O.E. Constantin). The objective of this study was to optimize the cultivation medium and growth conditions for “Bacillus licheniformis” DSM 5749 and “Bacillus subtilis” DSM 5750 isolated from a feed additive Bio Plus YC (Chr. Hansen). For the selection of significant variables for bacteria growth, a variety of carbon sources (glucose and xylooligosaccharides), inorganic salts (ammonium phosphate and magnesium sulfate), nitrogen source (yeast extract), and cultivation parameters (incubation period and inoculum size) were tested and identified via the Plackett–Burman design experiment. The results showed that the carbon source concentration and yeast extract had a positive effect on “Bacillus subtilis” growth. The carbon yeast extract, magnesium sulphate, XOS and inoculum size had a positive effect on “Bacillus licheniformis” growth. Significant negative effects on “Bacillus subtilis” growth had the ammonium phosphate and magnesium sulfate. The Pareto chart indicated that the order of effects for individual components that had a positive effect on the “Bacillus subtilis” and “Bacillus licheniformis” growth were the xylooligosaccharides (XOS) and incubation period. http://dx.doi.org/10.1016/j.jbiotec.2015.06.238 Physico-chemical, antioxidant activity and sensory analysis of new beverages based on milk, cranberry, rosehip fruits and horseradish Daniela Istrati ∗ , Cristina Pavalasc, Felicia Dima, Eugenia Pricop, Romulus Burluc, Camelia Vizireanu Food Science, Food Engineering, Food Biotechnology Department, Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, Romania E-mail address: [email protected] (D. Istrati). In the last few years are more potential demands on healthy and functional drinks due to the increase of the public awareness on the benefits of these products. Beverages based on fruits and milk products, besides their delicious taste, are highly nutritious because the fruits, vegetables and milk are the main sources of various bioactive compounds, natural antioxidants, as well as macro- and micro-
S77
ecological products enter in a large measure in the attention of specialized organisms, and also in the field of fundamental and applicative researches, being a relative new field but of maximum importance to insure population health. The paper contains new contributions to study the market of agricultural and food products on ecological food section, on consumers’ behaviours towards these products (knowledge, ignorance, tendency to consume these products or not, reaction to price etc.) by applying a questionnaire. Processing the data from questionnaires and establishing the requested correlations allow us to create an actual image upon the demand, selling and consumption of ecological products in Timis county.
and the environment. Regarding risk identification, were also used the references of World Health Organization, which has a special program to assist national authorities to identifying the risks associated with genetically modified organisms. Obtaining different genetically modified organisms, involves inserting different genes and different transfer techniques. Because of this, that risk analysis, about the safety of these foods, has analyzed on a case by case basis. Related to this, research showed that not exist until now recorded information showing that there was harmful effects on human health from the consumption of these foods.
http://dx.doi.org/10.1016/j.jbiotec.2015.06.235
Optimisation of growth condition for “Bacillus licheniformis” and “Bacillus subtilis” by using xylooligosaccharides
Research on spectroscopic composition of safflower seeds Carthamus tinctorius L. grown in the conditions of Southeastern Romania Dobrin Aurora 1,∗ , Marin Doru Ioan 1 , Manolache Fulvia Ancut¸a 2 , Ionescu Valentin 2 1 University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania 2 National Research & Development Institute for Food Bioresources – IBA Bucharest, Romania
E-mail address: aur ora [email protected] (D. Aurora). Safflower is a crop with importance for multiple uses: food, pharmaceuticals, cosmetics, paints and varnishes, and animal feed. Research has been carried out in Southeastern Romania (Moara Domneasc˘a Didactic Farm – 44◦ 29 33 N, 26◦ 15 20 E) in order to assess the suitability of cultivation in that region, and to analyze the quality of achenes and oils produced. The safflower cultivars were CW 88 OL, CW 1221 and Zanzibar. The research results show that the safflower adapted well to the soil under the experimental field conditions. Achenes yield was highest in all three varieties for the fertilizer level with N90P60K60, being 1878.33 kg ha−1 in the CW 1221 variety, 2064.32 kg ha−1 in the CW 88 OL variety and 2184.20 kg ha−1 in the Zanzibar variety. Safflower oil was extracted by Soxhlet method. Based on 1HNMR spectroscopy and using systems of chemometric equations, the composition of safflower oils was established on four classes of fatty acids: tri-unsaturated (undetectable for all varieties), di-unsaturated (the highest value was for Zanzibar: 75.05%), monounsaturated (the highest value was for CW 88 OL: 74.54%) and saturated (the highest value was for CW 88 OL: 11.29%). http://dx.doi.org/10.1016/j.jbiotec.2015.06.236 Achieving a risk analysis for products derived from genetically modified organisms, in terms of food safety Magda Gonciarov 1,∗ , Cristin Coman 2 1
Department of Animal Production and Public Health, Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania 2 Cantacuzino Institute, Bucharest, Romania E-mail address: [email protected] (M. Gonciarov). In the experiment realized by the authors of this paper, have been followed the steps of risk analysis, namely the identification, assessment, management and risk communication, in case of genetically modified organisms, in their relation to animal and human health,
http://dx.doi.org/10.1016/j.jbiotec.2015.06.237
Oana Emilia Constantin ∗ , Daniela Istrati, Cristian Teodor Buruiana, Camelia Vizireanu Food Science, Food Engineering, Food Biotechnology Department, Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, Romania E-mail address: [email protected] (O.E. Constantin). The objective of this study was to optimize the cultivation medium and growth conditions for “Bacillus licheniformis” DSM 5749 and “Bacillus subtilis” DSM 5750 isolated from a feed additive Bio Plus YC (Chr. Hansen). For the selection of significant variables for bacteria growth, a variety of carbon sources (glucose and xylooligosaccharides), inorganic salts (ammonium phosphate and magnesium sulfate), nitrogen source (yeast extract), and cultivation parameters (incubation period and inoculum size) were tested and identified via the Plackett–Burman design experiment. The results showed that the carbon source concentration and yeast extract had a positive effect on “Bacillus subtilis” growth. The carbon yeast extract, magnesium sulphate, XOS and inoculum size had a positive effect on “Bacillus licheniformis” growth. Significant negative effects on “Bacillus subtilis” growth had the ammonium phosphate and magnesium sulfate. The Pareto chart indicated that the order of effects for individual components that had a positive effect on the “Bacillus subtilis” and “Bacillus licheniformis” growth were the xylooligosaccharides (XOS) and incubation period. http://dx.doi.org/10.1016/j.jbiotec.2015.06.238 Physico-chemical, antioxidant activity and sensory analysis of new beverages based on milk, cranberry, rosehip fruits and horseradish Daniela Istrati ∗ , Cristina Pavalasc, Felicia Dima, Eugenia Pricop, Romulus Burluc, Camelia Vizireanu Food Science, Food Engineering, Food Biotechnology Department, Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, Romania E-mail address: [email protected] (D. Istrati). In the last few years are more potential demands on healthy and functional drinks due to the increase of the public awareness on the benefits of these products. Beverages based on fruits and milk products, besides their delicious taste, are highly nutritious because the fruits, vegetables and milk are the main sources of various bioactive compounds, natural antioxidants, as well as macro- and micro-