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Optimization of medium components for phytase production from Ochrobactrum anthropi
S72
Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
formation until the species colonization and stabilization of interspecific relationships which is favorable for the productivity and for the biocenotic balance of the new ecosystem. Acknowledgements: This work was published during the project POSDRU/89/1.5/S/62371.
Petri dishes. Five strains were found to be tolerant to all the tested concentrations. The clofibric acid elimination was studied by cultivation in submerged conditions in liquid basal medium supplemented with glucose as a co-substrate. Among the five tested strains only one strain found to lead to residual clofibric acid concentration lower than the fixed threshold.
http://dx.doi.org/10.1016/j.copbio.2013.05.198 http://dx.doi.org/10.1016/j.copbio.2013.05.200 Influence of organic and mineral fertilisation on a permanent grassland biodiversity and floristic composition Coman Marius Lucian, Moisuc Alexandu Gavril Banat’s University of Agricultural Sciences and Veterinary Medicine from Timisoara, Romania E-mail address: [email protected] (C.M. Lucian). The aim of this research is to highlight the changes that appear in the vegetation cover of grassland fertilised with different doses of solid manure. The analysed parameters are the level of biodiversity and floristic composition of the meadow. The dominant species of the grassland is Festuca arundinaceae and Agropyron repens. The experimental field is located in Fibis (Timis County, Romania), and the data are collected during 2010–2012 period. The experimental field was organized by setting 10 fertilisation variants (3 organic, 3 mineral and 3 organic — mineral fertilisation variants) and a control non-fertilised variant. Every studied variant has three replicates. The method of vegetation analysis is linear quadrate method. The vegetation features taken in account were: the botanical composition, the biodiversity (species richness, Shannon and Simpson indexes), some ecological indexes (temperature, humidity, soil reaction and light). The fertilisation has influenced mainly the biodiversity and the light and soil reaction ecological spectre. Regarding the ecological indexes, the greatest influence was evidence d in the case of light and soil reaction ecological spectres. It is recommended to be applied low fertilisation doses that will not affect the biodiversity and structure of the grassland vegetation. http://dx.doi.org/10.1016/j.copbio.2013.05.199 Study Streptomycetes as agents for clofibric acid biotransformation Popa Claudia 1 , Favier Lidia 2 , Bahrim Gabriela 1 , Amrane Abdeltif 2 1 Dunarea de Jos University, Faculty of Food Science and Engineering, 47 Domneasca St., 800008 Galati, Romania 2 Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, Université Européenne de Bretagne, General Leclerc Av., CS 50837, 35708 Rennes Cedex 7, France E-mail address: [email protected] (P. Claudia).
Previous studies indicated that filamentous bacteria have a great potential in polluted site bioremediation, especially in soil and aquatic systems. Clofibric acid is the main pharmacologically active metabolite of the blood lipid. It could be present in the aquatic medium as recalcitrant xenobiotic pollutant. The aim of this study was to test Streptomyces strains ability to transform clofibric acid in order to establish their resistance of target compound toxicity and evaluation of rate of biotransformation during submerged cultivation. Nineteen strains of actinomycetes belong of genus Streptomyces spp., isolated from soil samples were tested in specific microbiological conditions and selected as agent for clofibric acid biotransformation. Preliminary qualitative screening assays regarding testing resistance of bacteria against clofibric acid toxicity were carried out by cultivation on Gauze agar media supplemented with different concentrations of clofibric acid in
Optimization of medium components for phytase production from Ochrobactrum anthropi Afet Arkut, Melis Sümengen, Sadık Dinc¸er Biology Department, Faculty of Science and Letters, Cukurova University, Adana, Turkey E-mail address: [email protected] (S. Dinc¸er). Ochrobactrum anthropi, an isolate from wastewater treatment plant, produces phytase, which catalyzes the hydrolysis of phytate. The culture conditions were optimized for maximum enzyme production. The maximum production of phytase by this isolated was obtained after 48 hours incubation at 37◦ C and 300 rpm shaking. The best carbon and nitrogen sources for maximum phytase production were 1% maltose and 1% yeast extract, respectively. The enzyme was stable between the pH 6.5 and 9.0 but the optimal pH was found to be 8.5. The enzyme was also stable between temperature ranges 25◦ C and 55◦ C but the best temperature for enzyme activity was found to be 37◦ C. In addition the age and amount of the inoculum was investigated. As a result nine hours and OD600 = 0.06 were found, respectively. Maximum phytase activity was 343.44 U/ml after 48 hours of incubation under optimal conditions. http://dx.doi.org/10.1016/j.copbio.2013.05.201 Investigation of the antimicrobial activity of extracts from indigenous Xanthium strumarium plants against Phytophthora infestans Steliana Rodino 1,2 , Alina Butu 1 , Georgeta Fidler 1,2 , Marian Butu 1 , Petruta Calina Cornea 2 1 National Institute of Research and Development for Biological Sciences, Bucharest, Romania 2 University of Agronomic Sciences and Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (S. Rodino).
Currently, late blight continues to be an important disease, devastating the horticultural crops in Romania. Biological control of plant pathogens can lead to an increased ecological quality of the yields, having better effects on the environment, also. The aim of our study was to investigate the antimicrobial effect of hydroalcoholic extract obtained from dried aerial parts of Xanthium strumarium against Phytophthora infestans, the causative agent of late blight in tomatoes and potatoes. The extracts used in the experiments were obtained initially from whole plant and afterwards from leaves, stems, and fruits, by cold maceration in 70% ethylic alcohol. The positive control used was Ridomil Gold Plus 42,5 WP and the negative control was the ethylic alcohol. The antimicrobial activity was tested with the disk diffusion method and confirmed by well diffusion assay. By the development of the inhibition zones, the experiments showed that the extracts obtained from indigenous cocklebur reduced the growth of P. infestans mycelium. These preliminary results encourage the hypothesis that the plant extracts from Xanthium strumarium (a medicinal plant commonly found
Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
formation until the species colonization and stabilization of interspecific relationships which is favorable for the productivity and for the biocenotic balance of the new ecosystem. Acknowledgements: This work was published during the project POSDRU/89/1.5/S/62371.
Petri dishes. Five strains were found to be tolerant to all the tested concentrations. The clofibric acid elimination was studied by cultivation in submerged conditions in liquid basal medium supplemented with glucose as a co-substrate. Among the five tested strains only one strain found to lead to residual clofibric acid concentration lower than the fixed threshold.
http://dx.doi.org/10.1016/j.copbio.2013.05.198 http://dx.doi.org/10.1016/j.copbio.2013.05.200 Influence of organic and mineral fertilisation on a permanent grassland biodiversity and floristic composition Coman Marius Lucian, Moisuc Alexandu Gavril Banat’s University of Agricultural Sciences and Veterinary Medicine from Timisoara, Romania E-mail address: [email protected] (C.M. Lucian). The aim of this research is to highlight the changes that appear in the vegetation cover of grassland fertilised with different doses of solid manure. The analysed parameters are the level of biodiversity and floristic composition of the meadow. The dominant species of the grassland is Festuca arundinaceae and Agropyron repens. The experimental field is located in Fibis (Timis County, Romania), and the data are collected during 2010–2012 period. The experimental field was organized by setting 10 fertilisation variants (3 organic, 3 mineral and 3 organic — mineral fertilisation variants) and a control non-fertilised variant. Every studied variant has three replicates. The method of vegetation analysis is linear quadrate method. The vegetation features taken in account were: the botanical composition, the biodiversity (species richness, Shannon and Simpson indexes), some ecological indexes (temperature, humidity, soil reaction and light). The fertilisation has influenced mainly the biodiversity and the light and soil reaction ecological spectre. Regarding the ecological indexes, the greatest influence was evidence d in the case of light and soil reaction ecological spectres. It is recommended to be applied low fertilisation doses that will not affect the biodiversity and structure of the grassland vegetation. http://dx.doi.org/10.1016/j.copbio.2013.05.199 Study Streptomycetes as agents for clofibric acid biotransformation Popa Claudia 1 , Favier Lidia 2 , Bahrim Gabriela 1 , Amrane Abdeltif 2 1 Dunarea de Jos University, Faculty of Food Science and Engineering, 47 Domneasca St., 800008 Galati, Romania 2 Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, Université Européenne de Bretagne, General Leclerc Av., CS 50837, 35708 Rennes Cedex 7, France E-mail address: [email protected] (P. Claudia).
Previous studies indicated that filamentous bacteria have a great potential in polluted site bioremediation, especially in soil and aquatic systems. Clofibric acid is the main pharmacologically active metabolite of the blood lipid. It could be present in the aquatic medium as recalcitrant xenobiotic pollutant. The aim of this study was to test Streptomyces strains ability to transform clofibric acid in order to establish their resistance of target compound toxicity and evaluation of rate of biotransformation during submerged cultivation. Nineteen strains of actinomycetes belong of genus Streptomyces spp., isolated from soil samples were tested in specific microbiological conditions and selected as agent for clofibric acid biotransformation. Preliminary qualitative screening assays regarding testing resistance of bacteria against clofibric acid toxicity were carried out by cultivation on Gauze agar media supplemented with different concentrations of clofibric acid in
Optimization of medium components for phytase production from Ochrobactrum anthropi Afet Arkut, Melis Sümengen, Sadık Dinc¸er Biology Department, Faculty of Science and Letters, Cukurova University, Adana, Turkey E-mail address: [email protected] (S. Dinc¸er). Ochrobactrum anthropi, an isolate from wastewater treatment plant, produces phytase, which catalyzes the hydrolysis of phytate. The culture conditions were optimized for maximum enzyme production. The maximum production of phytase by this isolated was obtained after 48 hours incubation at 37◦ C and 300 rpm shaking. The best carbon and nitrogen sources for maximum phytase production were 1% maltose and 1% yeast extract, respectively. The enzyme was stable between the pH 6.5 and 9.0 but the optimal pH was found to be 8.5. The enzyme was also stable between temperature ranges 25◦ C and 55◦ C but the best temperature for enzyme activity was found to be 37◦ C. In addition the age and amount of the inoculum was investigated. As a result nine hours and OD600 = 0.06 were found, respectively. Maximum phytase activity was 343.44 U/ml after 48 hours of incubation under optimal conditions. http://dx.doi.org/10.1016/j.copbio.2013.05.201 Investigation of the antimicrobial activity of extracts from indigenous Xanthium strumarium plants against Phytophthora infestans Steliana Rodino 1,2 , Alina Butu 1 , Georgeta Fidler 1,2 , Marian Butu 1 , Petruta Calina Cornea 2 1 National Institute of Research and Development for Biological Sciences, Bucharest, Romania 2 University of Agronomic Sciences and Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (S. Rodino).
Currently, late blight continues to be an important disease, devastating the horticultural crops in Romania. Biological control of plant pathogens can lead to an increased ecological quality of the yields, having better effects on the environment, also. The aim of our study was to investigate the antimicrobial effect of hydroalcoholic extract obtained from dried aerial parts of Xanthium strumarium against Phytophthora infestans, the causative agent of late blight in tomatoes and potatoes. The extracts used in the experiments were obtained initially from whole plant and afterwards from leaves, stems, and fruits, by cold maceration in 70% ethylic alcohol. The positive control used was Ridomil Gold Plus 42,5 WP and the negative control was the ethylic alcohol. The antimicrobial activity was tested with the disk diffusion method and confirmed by well diffusion assay. By the development of the inhibition zones, the experiments showed that the extracts obtained from indigenous cocklebur reduced the growth of P. infestans mycelium. These preliminary results encourage the hypothesis that the plant extracts from Xanthium strumarium (a medicinal plant commonly found