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ORAL CAVITY AS RESPIRATORY PATHOGENS RESERVOIR ASSOCIATED WITH VENTILATOR-ASSOCIATED PNEUMONIA
OOOO Volume 124, Number 2 RP117 - ORAL CAVITY AS RESPIRATORY PATHOGENS RESERVOIR ASSOCIATED WITH VENTILATOR-ASSOCIATED PNEUMONIA. FERNANDA FERREIRA LOPES, LUANA CARNEIRO DINIZ SOUZA, MAURÍCIO PEREIRA MACEDO, MARIA CARMEN FONTOURA NOGUEIRA DA CRUZ, VANISE BARROS RODRIGUES DA MOTA, ALÍCIA VALÉRIA DOS SANTOS ZARANZA DE CARVALHO, RITA DA GRAÇA CARVALHAL FRAZÃO CORRÊA. The aim of this study was to identify respiratory pathogens in oral biofilm of intubated patients to detect possible association between respiratory pathogens from tracheal aspirate and oral biofilm of patients on mechanical ventilation, as well as, check the cases who developed ventilator-associated pneumonia (VAP). Collection of tongue biofilm in 32 intubated patients at a general/adult Intensive Care Unit was conducted to Culture Exam with Antibiotic Sensitivity Test and it was compared with samples of tracheal secretions. The results showed that 19 (59.37%) patients exhibited the same species of pathogens in tracheal aspirate and oral biofilm, from these, 8 (42.1%) developed VAP, after 2 days of admission. Pseudomonas aeruginosa was the most common respiratory pathogens detected in oral biofilm of patients with VAP. There was significant association between the species of microorganisms in tracheal and mouth collection for these pathogens: Klebsiella pneumoniae, Candida albicans, Pseudomonas aeruginosa, Enterobacter gergoviae, Streptococcus spp and Serratia marcescens (exact Fisher test, I<.05). It was concluded that the same pathogens that are present in the tracheal secretion of intubated patients can be detected in the oral cavity, so, the oral biofilm can be a reservoir of respiratory pathogens for patients on mechanical ventilation.
RP118 - TOBACCO USE AND CESSATION KNOWLEDGE AMONG DENTAL STUDENTS AND DENTISTS. FLÁVIA HELENA ALVES FERREIRA, LETÍCIA MARIA BONFIM RODRIGUES, CELINA FAIG LIMA, DENISE NICODEMO, JANETE DIAS ALMEIDA, SYMONE CRISTINA TEIXEIRA. The aim of this study was to evaluate tobacco consumption knowledge among dental students and dentists, and whether the smoker’s patients receive adequate motivation for smoking cessation. Study Design: This questionnaire-based study was carried out among 181 dental students and 209 dentists. Two questionnaires (Q) were used to assess data about knowledge and attitudes toward tobacco use and cessation. Q1: consisted of 14 questions about tobacco consumption knowledge and Q2: consisted of 16 questions about attitudes towards tobacco use and cessation. Results: Q1 results demonstrated knowledge regarding the relationship between smoking and oral cancer. Q2 answers showed that 23.65% dental students and 23.92% dentists are uncomfortable to provide smoking cessation advice to their patients. Lack of knowledge about smoking related risks and counseling for smoking cessation was reported by 94.16% dental students and 98.8% dentists. Conclusions: This study highlights the need and importance of education and practical training of undergraduate dental students in tobacco prevention and cessation skills.
RP119 - BUCCAL HEALTH CONDITION AND PROFILE FROM DENTISTRY GRADUATING STUDENTS WHO USE ALCOHOL AND/ OR TOBACCO. SANDY MARIA DA SILVA COSTA, DIEGO BRUNO OLIVEIRA MORAIS, DAVID SALDANHA DE
ABSTRACTS Abstracts e149 BRITO ALENCAR, SÂMMEA MARTINS VIERIA, ERIKA LOUIZE DE CARVALHO GOMES, MÁRCIA VALÉRIA MARTINS. Given that the abusive consumption of alcohol and/or tobacco can cause bucco-dental alterations, it has become important the fulfillment of this work, which its goals are to evaluate the prevalence of bucco-dental alterations in dentistry graduating students, who have consumed alcoholic drinks and/or tobacco, to identify pathologies related to the consumption of these substances, in different areas of the buccal mucosa, as well as, to check possible dental alterations. A descriptive study was done, through data collection, with quantitative approach, using a survey with 8 questions that was delivered to 73 students enrolled between the first and tenth semester, from August to December of 2013. Those students were submitted to 3 intraoral clinical examinations, during 15 days. Data analysis was obtained using descriptive statistics for percentage calculation in base 100. After the data collect, it revealed that 69.86% used alcohol and/or tobacco, of which 60.78% were male; the most consumed beverage was beer (78%) and only 5% of them used tobacco. Concluding in, 22% of the survey participants presented some buccodental alteration, of which 66.67% observed in the jugal mucosa (leukoplasias), tongue (leukoplakia), and gingival tissues (gingivitis), and 33.33% observes on the teeth (caries and plaque).
RP120 - IMPACT OF METALLOTHIONEIN GENE POLYMORPHISMS ON THE RISK OF ORAL SQUAMOUS CELL CARCINOMA IN A BRAZILIAN POPULATION. SÉRGIO VITORINO CARDOSO, ROBERTA REZENDE ROSA, MARCELO AUGUSTO GARCIA-JÚNIOR, ADRIANO MOTA LOYOLA, LUIZ RICARDO GOULART, PAULO ROGÉRIO DE FARIA, PAULA CRISTINA BATISTA DE FARIA. This work aimed to investigate the possible association between the risk of oral squamous cell carcinoma (OSCC) and the frequency of single-nucleotide polymorphisms (SNPs) related to metallothionein genes in a Brazilian population-based case-control study. The group of cases consisted of 28 patients, with 45 unrelated healthy volunteers included in the control group. All participants provided blood samples as well as information on risk factors for OSCC, including tobacco and alcohol consumption. Genotyping was performed by PCR-RFLP analysis to determine the SNPs rs8052334 (MT1-B gene), rs964372 (MT-1B) and rs1610216 (MT2-A), or by TaqMan single-nucleotide polymorphism genotyping assays for rs11076161 (MT-1A). Polymorphism in MT-1A gene was associated with increased risk for OSCC, since carriers of the G allele were less prone to experience OSCC development than subjects with the ancestral AA genotype (P < .05). No significant association was observed between the SNPs rs1610216, rs964372 or rs8052334 and the OSCC occurrence. Haplotype analysis of MT genetic polymorphisms did not reveal strong linkage disequilibrium in haplotype block with the selected SNPs. In conclusion, genetic polymorphisms in the MT-1A gene affect susceptibility to oral cancer. Financial support: CNPq, CAPES, FAPEMIG.
RP121 - A 6-YEAR EXPERIENCE OF A SPECIALIZED CENTER IN THE MULTIDISCIPLINARY TREATMENT OF HEAD AND NECK CANCER PATIENTS. JANAÍNA ZAVITOSKI DA SILVA, SUZY ELAINE NOBRE FREITAS, DANIELA BRITO BASTOS, INGRID DA SILVA SANTOS, DANIEL GALERA BERNABÉ, ÉDER RICARDO BIASOLI, GLAUCO ISSAMU MIYAHARA.
RP118 - TOBACCO USE AND CESSATION KNOWLEDGE AMONG DENTAL STUDENTS AND DENTISTS. FLÁVIA HELENA ALVES FERREIRA, LETÍCIA MARIA BONFIM RODRIGUES, CELINA FAIG LIMA, DENISE NICODEMO, JANETE DIAS ALMEIDA, SYMONE CRISTINA TEIXEIRA. The aim of this study was to evaluate tobacco consumption knowledge among dental students and dentists, and whether the smoker’s patients receive adequate motivation for smoking cessation. Study Design: This questionnaire-based study was carried out among 181 dental students and 209 dentists. Two questionnaires (Q) were used to assess data about knowledge and attitudes toward tobacco use and cessation. Q1: consisted of 14 questions about tobacco consumption knowledge and Q2: consisted of 16 questions about attitudes towards tobacco use and cessation. Results: Q1 results demonstrated knowledge regarding the relationship between smoking and oral cancer. Q2 answers showed that 23.65% dental students and 23.92% dentists are uncomfortable to provide smoking cessation advice to their patients. Lack of knowledge about smoking related risks and counseling for smoking cessation was reported by 94.16% dental students and 98.8% dentists. Conclusions: This study highlights the need and importance of education and practical training of undergraduate dental students in tobacco prevention and cessation skills.
RP119 - BUCCAL HEALTH CONDITION AND PROFILE FROM DENTISTRY GRADUATING STUDENTS WHO USE ALCOHOL AND/ OR TOBACCO. SANDY MARIA DA SILVA COSTA, DIEGO BRUNO OLIVEIRA MORAIS, DAVID SALDANHA DE
ABSTRACTS Abstracts e149 BRITO ALENCAR, SÂMMEA MARTINS VIERIA, ERIKA LOUIZE DE CARVALHO GOMES, MÁRCIA VALÉRIA MARTINS. Given that the abusive consumption of alcohol and/or tobacco can cause bucco-dental alterations, it has become important the fulfillment of this work, which its goals are to evaluate the prevalence of bucco-dental alterations in dentistry graduating students, who have consumed alcoholic drinks and/or tobacco, to identify pathologies related to the consumption of these substances, in different areas of the buccal mucosa, as well as, to check possible dental alterations. A descriptive study was done, through data collection, with quantitative approach, using a survey with 8 questions that was delivered to 73 students enrolled between the first and tenth semester, from August to December of 2013. Those students were submitted to 3 intraoral clinical examinations, during 15 days. Data analysis was obtained using descriptive statistics for percentage calculation in base 100. After the data collect, it revealed that 69.86% used alcohol and/or tobacco, of which 60.78% were male; the most consumed beverage was beer (78%) and only 5% of them used tobacco. Concluding in, 22% of the survey participants presented some buccodental alteration, of which 66.67% observed in the jugal mucosa (leukoplasias), tongue (leukoplakia), and gingival tissues (gingivitis), and 33.33% observes on the teeth (caries and plaque).
RP120 - IMPACT OF METALLOTHIONEIN GENE POLYMORPHISMS ON THE RISK OF ORAL SQUAMOUS CELL CARCINOMA IN A BRAZILIAN POPULATION. SÉRGIO VITORINO CARDOSO, ROBERTA REZENDE ROSA, MARCELO AUGUSTO GARCIA-JÚNIOR, ADRIANO MOTA LOYOLA, LUIZ RICARDO GOULART, PAULO ROGÉRIO DE FARIA, PAULA CRISTINA BATISTA DE FARIA. This work aimed to investigate the possible association between the risk of oral squamous cell carcinoma (OSCC) and the frequency of single-nucleotide polymorphisms (SNPs) related to metallothionein genes in a Brazilian population-based case-control study. The group of cases consisted of 28 patients, with 45 unrelated healthy volunteers included in the control group. All participants provided blood samples as well as information on risk factors for OSCC, including tobacco and alcohol consumption. Genotyping was performed by PCR-RFLP analysis to determine the SNPs rs8052334 (MT1-B gene), rs964372 (MT-1B) and rs1610216 (MT2-A), or by TaqMan single-nucleotide polymorphism genotyping assays for rs11076161 (MT-1A). Polymorphism in MT-1A gene was associated with increased risk for OSCC, since carriers of the G allele were less prone to experience OSCC development than subjects with the ancestral AA genotype (P < .05). No significant association was observed between the SNPs rs1610216, rs964372 or rs8052334 and the OSCC occurrence. Haplotype analysis of MT genetic polymorphisms did not reveal strong linkage disequilibrium in haplotype block with the selected SNPs. In conclusion, genetic polymorphisms in the MT-1A gene affect susceptibility to oral cancer. Financial support: CNPq, CAPES, FAPEMIG.
RP121 - A 6-YEAR EXPERIENCE OF A SPECIALIZED CENTER IN THE MULTIDISCIPLINARY TREATMENT OF HEAD AND NECK CANCER PATIENTS. JANAÍNA ZAVITOSKI DA SILVA, SUZY ELAINE NOBRE FREITAS, DANIELA BRITO BASTOS, INGRID DA SILVA SANTOS, DANIEL GALERA BERNABÉ, ÉDER RICARDO BIASOLI, GLAUCO ISSAMU MIYAHARA.