ORAL CHANGES IN CHILDREN WITH ZIKA VIRUS CONGENITAL SYNDROME

OOOO Volume 129, Number 1 Objective: Evaluate the presence and viral load of cytomegalovirus (CMV) and Epstein-Barr virus (EBV) through the real-time polymerase chain reaction (PCR) technique. Study design: Epidemiologic and observational study, with the presence of 2 groups: hemodialysis and control (healthy as chronic kidney disease). All patients responded to a clinical file and submitted to a clinical examination of the oral cavity to evaluate the presence of oral lesions compatible with the presence of the virus studied. Subsequently, 3 mL of blood were collected, from which the DNA was extracted and the viral load of CMV and EBV was obtained. Results: In the hemodialysis group, 10 patients (18.51%) were positive for this virus, with a mean viral load of 196.4 copies/mL. In the control group, 8 (14.54%) were positive, with a mean viral load of 34.7 copies/mL, with no significant difference. Regarding CMV, the control group did not present any patients with detectable viral load, while in the hemodialysis group 29 patients were positive, with a mean viral load of 265,282.8 copies/mL. No patient had systemic or oral manifestations characteristic of viral diseases. Conclusions: Relative immunosuppression due to hemodialysis was not significant to the point of initiating oral and systemic alterations.

FASN AND GLUT-1 EXPRESSION IN THE PLEOMORPHIC ADENOMA AND CARCINOMA EX-PLEOMORPHIC ADENOMA. e JOAO FIGUEIRA SCARINI, LOUYSE VIZOTTO  CARVALHO, LARISSA FERNANDES, DEBORA CAMPANELLA BASTOS, ALBINA ALTEMANI, LUIZ PAULO KOWALSKI and, FERNANDA VIVIANE MARIANO Carcinoma ex-pleomorphic adenoma (CXPA) arises in pleomorphic adenoma (PA) and with an increase in lipogenesis during its malignant transformation. The increase in lipogenesis is a feature of the cancer cells and it is characterized by the increase of fatty acid (FA) synthesis. This way, the overexpression of the enzyme fatty acid synthase (fasn) has been correlated. In the same way, the increase of glycolytic metabolism in neoplastic cells is due to the high expression of glycolytic enzymes and glucose transporters (gluts). Objective: Compare the fasn and glut-1 expression between CXPAs and PAs samples. Study Design: Forty PA and 40 CXPA were analyzed by immunohistochemistry with fasn and glut-1 antibodies. The proportion of positive neoplastic cells was assessed according to a 2-tiered scale: >10% to 50%, and >50% positive cells. Results: Fifteen PA (37.5%) and 30 CXPA (75%) were fasn positive. Twenty PA (20%) and 29 CXPA (70%) were glut-1 positive. Conclusions: The present results suggest that fasn and glut1 enzyme overexpression are associated with the malignant transformation of the PA. This way, the increase of the lipogenesis and glycolytic metabolism should be collaborative factors for carcinogenesis since cancer requires a highest demand of energy for survival and progression.

CONSERVATIVE APPROACH OF DENTIGEROUS CYST WITH ERUPTION OF CYSTASSOCIATED TEETH: A CASE SERIES.  RENATA GON¸CALVES RESENDE, SERGIO HENRIQUE

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TANOS DE LACERDA, ALINE FERNANDA CRUZ, ISABELA FERNANDES, BRUNO MOREIRA, BRENDA   STEFANE COSTA and, JULIO CESAR TANOS DE LACERDA To present a series of clinical cases of dentigerous cysts treated by means of marsupialization using the retained teeth associated with the lesion. This is an epidemiologic study based on the retrospective and descriptive analysis from November 2005 to November 2015. Twelve patients were included in the study, with mean age of 9 years. Eight patients were men and 4 were women. The most involved teeth were premolars and maxillary central incisors and the mean eruption time was 13 months. In all cases there was eruption of the associated tooth and new bone formation in the area where the cyst was present. No recurrences were observed. In the cases treated in the evaluated period, marsupialization was shown as an effective therapeutic option in the approach of young patients.

ORAL CHANGES IN CHILDREN WITH ZIKA VIRUS CONGENITAL SYNDROME. TERESA e PAULA DE LIMA GUSMAO, ANDREZA BARKOKEBAS SANTOS DE FARIA, ADRIANA MACHADO ZARZAR, IGOR HENRIQUE MORAIS SILVA, LUIZ ALCINO MONTEIRO GUEIROS, ALESSANDRA DE ALBUQUERQUE TAVARES e CARVALHO and, JAIR CARNEIRO LEAO Objective: To describe oral alterations in children with Zika congenital syndrome (ZCS). Study Design: A cross-sectional study, whose research instrument was a structured questionnaire, associated with the use of medical record data and extra and intraoral clinical examination. Results: Thirty-two children were evaluated, the majority male (18/32, 56.3%) with a mean age in months § SD of 22 § 2.71. It was also observed that the majority of the patients (19/ 32, 59.4%) presented from low-income families. All the children had intracranial calcifications and a mean head circumference § SD of 29.43 § 1.42. As for functional difficulties, 12 children (37.5%) were unable to breast-feed and 24 (75%) had dysphagia. An ogival-shaped palate was observed in 14 children (43.7%) and their correlation with dysphagia was also not statistically significant (P = .09). Regarding the oral alterations, delayed chronology of eruption was observed in 15 children (46.9%), of whom 7 children (21.9%) did not present eruption of the upper left lateral incisor (P = .0002) and right (P = .002) until the moment of analysis. Enamel hypoplasia was identified in 9 children (28.1%) and only 1 child with ankyloglossia. Conclusions: ZCS may present with oral alterations requiring dental follow-up care aimed at prevention, promotion, and rehabilitation of the health of these children.

DIAGNOSTIC MODEL OF EARLY STAGE OF ORAL CANCER BASED ON SINGLE NUCLEOTIDE POLYMORPHISMS. MARIA  FERNANDA GALINDEZ COSTA, VICTORIANO ANDRES CARRICA, IGNACIO GONZALEZ SEGURA, RENE PANICO, JOSE BARRA, DANTE GUSTAVO SECCHI and, MABEL BRUNOTTO