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Oscillatory convective flow can accelerate signal propagation in epithelium
Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
Oscillatory convective flow can accelerate signal propagation in epithelium Marek Nebyla, Michal Pribyl Department of Chemical Engineering, ICT Prague, Praha, Czech republic E-mail address: [email protected] (M. Nebyla). We developed and analyzed the mathematical model of signal propagation in epithelial tissues with the oscillatory flow of extracellular medium. The mathematical model consists of reaction-transport equations for extracellular ligands, ligandreleasing protease, free cellular receptors and ligand–receptor complexes. Diffusion and oscillatory convective transport of extracellular ligand are considered. Dynamic simulations showed that the oscillatory flow with the zero time-averaged flow rate accelerates the signal transmission in a large range of frequencies. We will present obtained nonmonotoneous frequency dependencies of the signal propagation velocity and they will be explained by means of asymptotic solutions. Our findings can be useful in vascular tissue or bone engineering. http://dx.doi.org/10.1016/j.copbio.2013.05.163 Bioprocess Engineering Adaptation of permanent fish cell line CCO for growth in serumfree medium Viˇsnja Gaurina Srcek, Kristina Radoˇsevic, Bogdanka Dukic University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia E-mail address: [email protected] (V.G. Srcek). The use of fish cell lines in biotechnology, ecotoxicology, virology and basic researches is constantly increasing. Proliferation of fish cells in culture is dependent on factors usually presented in serum of animal origin. Due to many obstacles regarding serum usage, serum-free media (SFM) are preferred, especially in the bioprocesses using animal cells. The aim of this study was to adapt fish CCO cells to grow in SFM by gradual reduction of serum content. During adaptation in UltraCulture medium (UCM) with 10–0% (v/v) foetal bovine serum (FBS), obtained cell yields were in the range of 17.8–2.8 × 105 cells/ml. The cryopreserved SFMadapted CCO cells were checked during 3 months of storage and could be revived with cell monolayer established within one week. Since CCO cells are used for Channel Catfish Virus (CCV) vaccine production, we also attempted to obtain higher cell yields in lowserum media. CCO cells adapted to 2% of FBS were cultivated on Cytodex 1 microcarriers in Cell-spin bioreactors for 13 days. Results showed slightly higher cell yield (17.0 × 105 cells/ml) in UCM with 2% FBS when compared to regular DMEM medium with 5% FBS (15.0 × 105 cells/ml). In conclusion, CCO cells were successfully adapted to grow in SFM but further optimization is needed for their efficient use in microcarriers/bioreactor system. http://dx.doi.org/10.1016/j.copbio.2013.05.166
S63
Application of the horizontal rotating tubular bioreactor for the biofuels production: Optimisation of the enzymatic lignocellulose material treatment and bioethanol production Tonci Rezic 1 , Iva Rezic 2 , Damir Oros 1 , Daniel Kracher 3 , Wanying Yao 4 , Roland Ludwig 3 , Bozidar Santek 1 1
Department of Biochemical Engineering, Laboratory for Biochemical Engineering, Industrial Microbiology and Brewery, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000, Croatia 2 Department of Applied Chemistry, Faculty of Textile Technology, University of Zagreb, Prilaz Baruna Filipovica 28a, 10000, Croatia 3 Laboratory of Food Biotechnology, Department of Food Sciences and Technology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria 4 Bioproducts and Biosystems Engineering Department, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, USA E-mail address: [email protected] (T. Rezic). The horizontal rotating tubular bioreactor (HRTB) was constructed to provide maximum efficiency and minimal energy consumption in the biofuels production processes. A model of the sugar beet pulp enzymatic treatment was established to predict optimal bioprocess performance in HRTB. Furthermore, Design of Experiment (DOE) was also apply to improve the optimisation and selection of the multi-enzyme system. Different DOEs (General, Two level fractorial, Box Behnken, and D-optimal designs) were preformed in order to optimise multi-enzyme system. Obtained results were used in the simulations of bioprocess performance in the HRTB by Response Surface Methodology (RSM). From obtained results it is evident that changes in the initial lignocellulose material concentration and bioreactor rotation speed significantly influence bioprocess efficiency. The highest ethanol yield was observed by 12 rpm and initial lignocellulose material concentration of 133 g/L. On the bases of DOE application and simulation of HRTB performance three assumptions made for HRTB application was confirmed: first, the high conversion yields, second, high degree of initial lignocellulose material hydrolysation, and third, reduction in enzyme amounts and costs. http://dx.doi.org/10.1016/j.copbio.2013.05.167 Bioengineered polymers to mimic anti-bodies’ molecular recognition Stefan Ovidiu Dima 1,2,3 1
University Politehnica From Bucharest, Romania Max Planck Institute for Colloids and Interfaces Potsdam, Germany 3 National Institute of Research and Development for Chemistry and Petrochemistry Icechim Bucharest, Romania E-mail address: [email protected]. 2
Molecularly imprinted polymers (MIPs) are bioengineered polymers obtained by inducing a molecular memory to a polymer matrix, procedure inspired by nature that is also known as configurational biomimesis. Molecular imprinting technique, similar to configurational biomimesis, implies the achieving of a threedimensional arrangement of chemical active groups around the template molecules via covalent, noncovalent, and ionic forces, or a combination of these. MIP area of applications is wide and expansive, including any domain that requires high affinity and selectivity. The aim of present work is to envisage the role of template molecule and the polymer matrix on the affinity and selectivity of our obtained MIPs. Eighteen different copolymer systems based on three monomers (acrylonitrile-AN: acrylic acid-AA
Oscillatory convective flow can accelerate signal propagation in epithelium Marek Nebyla, Michal Pribyl Department of Chemical Engineering, ICT Prague, Praha, Czech republic E-mail address: [email protected] (M. Nebyla). We developed and analyzed the mathematical model of signal propagation in epithelial tissues with the oscillatory flow of extracellular medium. The mathematical model consists of reaction-transport equations for extracellular ligands, ligandreleasing protease, free cellular receptors and ligand–receptor complexes. Diffusion and oscillatory convective transport of extracellular ligand are considered. Dynamic simulations showed that the oscillatory flow with the zero time-averaged flow rate accelerates the signal transmission in a large range of frequencies. We will present obtained nonmonotoneous frequency dependencies of the signal propagation velocity and they will be explained by means of asymptotic solutions. Our findings can be useful in vascular tissue or bone engineering. http://dx.doi.org/10.1016/j.copbio.2013.05.163 Bioprocess Engineering Adaptation of permanent fish cell line CCO for growth in serumfree medium Viˇsnja Gaurina Srcek, Kristina Radoˇsevic, Bogdanka Dukic University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia E-mail address: [email protected] (V.G. Srcek). The use of fish cell lines in biotechnology, ecotoxicology, virology and basic researches is constantly increasing. Proliferation of fish cells in culture is dependent on factors usually presented in serum of animal origin. Due to many obstacles regarding serum usage, serum-free media (SFM) are preferred, especially in the bioprocesses using animal cells. The aim of this study was to adapt fish CCO cells to grow in SFM by gradual reduction of serum content. During adaptation in UltraCulture medium (UCM) with 10–0% (v/v) foetal bovine serum (FBS), obtained cell yields were in the range of 17.8–2.8 × 105 cells/ml. The cryopreserved SFMadapted CCO cells were checked during 3 months of storage and could be revived with cell monolayer established within one week. Since CCO cells are used for Channel Catfish Virus (CCV) vaccine production, we also attempted to obtain higher cell yields in lowserum media. CCO cells adapted to 2% of FBS were cultivated on Cytodex 1 microcarriers in Cell-spin bioreactors for 13 days. Results showed slightly higher cell yield (17.0 × 105 cells/ml) in UCM with 2% FBS when compared to regular DMEM medium with 5% FBS (15.0 × 105 cells/ml). In conclusion, CCO cells were successfully adapted to grow in SFM but further optimization is needed for their efficient use in microcarriers/bioreactor system. http://dx.doi.org/10.1016/j.copbio.2013.05.166
S63
Application of the horizontal rotating tubular bioreactor for the biofuels production: Optimisation of the enzymatic lignocellulose material treatment and bioethanol production Tonci Rezic 1 , Iva Rezic 2 , Damir Oros 1 , Daniel Kracher 3 , Wanying Yao 4 , Roland Ludwig 3 , Bozidar Santek 1 1
Department of Biochemical Engineering, Laboratory for Biochemical Engineering, Industrial Microbiology and Brewery, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000, Croatia 2 Department of Applied Chemistry, Faculty of Textile Technology, University of Zagreb, Prilaz Baruna Filipovica 28a, 10000, Croatia 3 Laboratory of Food Biotechnology, Department of Food Sciences and Technology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria 4 Bioproducts and Biosystems Engineering Department, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, USA E-mail address: [email protected] (T. Rezic). The horizontal rotating tubular bioreactor (HRTB) was constructed to provide maximum efficiency and minimal energy consumption in the biofuels production processes. A model of the sugar beet pulp enzymatic treatment was established to predict optimal bioprocess performance in HRTB. Furthermore, Design of Experiment (DOE) was also apply to improve the optimisation and selection of the multi-enzyme system. Different DOEs (General, Two level fractorial, Box Behnken, and D-optimal designs) were preformed in order to optimise multi-enzyme system. Obtained results were used in the simulations of bioprocess performance in the HRTB by Response Surface Methodology (RSM). From obtained results it is evident that changes in the initial lignocellulose material concentration and bioreactor rotation speed significantly influence bioprocess efficiency. The highest ethanol yield was observed by 12 rpm and initial lignocellulose material concentration of 133 g/L. On the bases of DOE application and simulation of HRTB performance three assumptions made for HRTB application was confirmed: first, the high conversion yields, second, high degree of initial lignocellulose material hydrolysation, and third, reduction in enzyme amounts and costs. http://dx.doi.org/10.1016/j.copbio.2013.05.167 Bioengineered polymers to mimic anti-bodies’ molecular recognition Stefan Ovidiu Dima 1,2,3 1
University Politehnica From Bucharest, Romania Max Planck Institute for Colloids and Interfaces Potsdam, Germany 3 National Institute of Research and Development for Chemistry and Petrochemistry Icechim Bucharest, Romania E-mail address: [email protected]. 2
Molecularly imprinted polymers (MIPs) are bioengineered polymers obtained by inducing a molecular memory to a polymer matrix, procedure inspired by nature that is also known as configurational biomimesis. Molecular imprinting technique, similar to configurational biomimesis, implies the achieving of a threedimensional arrangement of chemical active groups around the template molecules via covalent, noncovalent, and ionic forces, or a combination of these. MIP area of applications is wide and expansive, including any domain that requires high affinity and selectivity. The aim of present work is to envisage the role of template molecule and the polymer matrix on the affinity and selectivity of our obtained MIPs. Eighteen different copolymer systems based on three monomers (acrylonitrile-AN: acrylic acid-AA