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Osteocalcin, n-telopeptides and beta crosslaps in Spanish adolescents
S86
Abstracts / Bone 45 (2009) S59–S111
PT-19 The serum concentration of the receptor activator of nuclear factor-kB ligand (RANKL), osteoprotegerin (OPG) and lipids profile in children with nephrotic syndrome M. Panczyk-Tomaszewskaa, H. Ziolkowskaa, D. Adamczuka, E. Górskab, A. Stelmaszczyk-Emmelb, M. Roszkowska-Blaima a Department of Pediatrics and Nephrology, Medical University of Warsaw, Poland, Warsaw, Poland b Department of Laboratory Diagnostics and Clinical Imuunology of Developmental Age, Medical University of Warsaw, Warsaw, Poland Selected data indicates that bone mineralisation and lipids metabolism may be related, but the exact mechanism remains unknown. Aim: The aim of the study was to assess the serum concentration of RANKL/OPG system and lipids metabolism parameters in children with different stages of nephrotic syndrome (NS). Material and methods: Twenty two children with NS in remission aged from 6.0 to 17 years mean age 10.9 ± 4.0; 12 treated with glucocorticoid and vitamin D (group I), 10 without treatment (group II) were involved into the study. The control group consisted of 16 healthy children, aged from 1.9 to 17.8 years, mean age 10.5 ± 5.0 years. In the study groups serum concentrations of OPG, RANKL, calcium, phosphorus, total cholesterol, HDL-cholesterol, LDL-cholesterol, triglycerides, albumin, creatinine, 25OH D3, and 1,25(OH)2D3 were measured. Results: The concentration of OPG in children with NS (groups I and II) was significantly lower compared to control group (from 0.8 to 4.9 median 1.65; from 0.5 to 3.7 median 1.40; from 1.1 to 4.9 median 3.05 respectively, p < 0.01). There were no significant differences between serum concentrations of RANKL in groups I, II and control group. In the study groups the significant negative correlation was found between concentrations of OPG and HDLcholesterol level (r = −0.63, p < 0.05). There was no significant correlation between OPG, RANKL and other studied parameters. Conclusion: Plasma OPG may be considered as a new biomarker of lipid abnormalities. doi:10.1016/j.bone.2009.04.121
PT-20 Osteocalcin, n-telopeptides and beta crosslaps in Spanish adolescents L. Gracia-Marcoa,b, G. Vicente-Rodrígueza,b, J. Valtueñac, J.P. Rey-Lópeza,b, E. DíazMartínezd, M.I. Mesanaa,b, K. Widhalme, J.R. Ruizf, M. González-Grossc,g, M.J. Castillog, L.A. Morenoa,b a GENUD: “Growth, Exercise, Nutrition and Development” Research Group, University of Zaragoza, Zaragoza, Spain b School of Health Sciences, Department of Physiotherapy and Nursing, University of Zaragoza, Zaragoza, Spain c Department of Health and Human Performance, Faculty of Physical Activity and Sport Sciences-INEF, Universidad Politécnica de Madrid, Madrid, Spain d Clinical Laboratory, Sport and Medicine Center, CSD, Madrid, Spain e Division of Clinical Nutrition and Prevention, Department of Pediatrics, Medical University of Vienna, Vienna, Austria f Unit for Preventive Nutrition, Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden g Institut für Ernährungs- und Lebensmittelwissenschaften, Humanernährung, Rheinische Friedrich-Wilhelms Universität, Bonn, Germany h Department of Physiology, School of Medicine, University of Granada, Granada, Spain Introduction and objectives: Osteocalcin, aminoterminal propeptide of type I procollagen (PINP) and beta crosslaps have been
described as markers of bone metabolism. Therefore, we aimed to describe the concentration of these markers during adolescence in male and female Spanish adolescents. Methods: Blood samples were obtained in 117 Spanish adolescents (53 males and 64 females), aged 12.5–17.5 years, within the framework of the HELENA study. Serum osteocalcin, PINP and beta crosslaps concentrations were determined by electrochemiluminescence immunoassay “ECLIA” by Elecsys 2010 analyzer. Subjects were categorized by age and Tanner stage, in four and three categories, respectively. One-way ANOVA was carried out to determine differences between age and/or Tanner groups. Independent Samples TTest was applied to determine gender differences. Results: PINP decreased with age in both males and females (p < 0.05). Males had significantly higher concentration of osteocalcin, PINP and beta crosslaps compared with females in all age categories (p < 0.05) except in the ranges 15–15.99 years and 16– 17.5 years (beta crosslaps and PINP, respectively). When the sample was categorized by Tanner stage (Table 1), osteocalcin, PINP and beta crosslap concentrations decreased with maturation in males, while only PINP decreased with maturation in females. Males had significantly higher concentration than females for beta crosslaps at all Tanner stages and osteocalcin and PINP for Tanner 5. Table 1 Bone markers concentration according to Tanner stage.
n TANNER
3 4 5
p
Male Osteocalcin (ng/mL)
PINP (ng/mL)
Beta crosslaps (ng/mL)
53 153.97 ± 46.47a 136.53 ± 54.94 100.54 ± 44.74⁎ 0.013
47 1185.9 ± 426.9a 878.35 ± 361.8b 501.9 ± 321.27⁎ 0.000
33 1.78 ± 0.18⁎ 2.09 ± 0.54b,⁎ 1.30 ± 0.44⁎ 0.002
Female Osteocalcin (ng/mL)
PINP (ng/mL)
Beta crosslaps (ng/mL)
64 99.48 ± 25.88 96.77 ± 43.03 58.44 ± 41.82 0.096
61 619.2 ± 21.9a 564.5 ± 359.5 259.2 ± 199.13 0.003
47 1.43 ± 0.19 0.98 ± 0.41 0.95 ± 0.59 0.394
⁎
p < 0.05 between gender; ap < 0.05 between Tanner 3 and 5; p < 0.05 between Tanner 4 and 5. Conclusions: Male adolescents showed higher concentrations of osteocalcin, PINP and beta crosslaps than females through adolescence. Bone turnover seems to decrease during adolescence, especially in males. b
doi:10.1016/j.bone.2009.04.122
PT-21 Bone markers and physical activity in Spanish adolescents L. Gracia-Marcoa,b, G. Vicente-Rodrígueza,b, J. Valtueñac, J.P. Rey-Lópeza,b, E. Díaz Martínezd, M.I. Mesanaa,b, K. Widhalme, J.R. Ruizf, M. González-Grossc,g, M. Sjöströmf, M.J. Castilloh, L.A. Morenoa,b a GENUD: “Growth, Exercise, Nutrition and Development” Research Group, University of Zaragoza, Zaragoza, Spain b School of Health Sciences, Department of Physiotherapy and Nursing, University of Zaragoza, Zaragoza, Spain c Department of Health and Human Performance, Faculty of Physical Activity and Sport Sciences-INEF, Universidad Politécnica de Madrid, Madrid, Spain d Clinical Laboratory, Sport and Medicine Center, CSD, Madrid, Spain e Division of Clinical Nutrition and Prevention, Department of Pediatrics, Medical University of Vienna, Vienna, Austria f Unit for Preventive Nutrition, Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden g Institut für Ernährungs- und Lebensmittelwissenschaften Humanernährung, Rheinische Friedrich-Wilhelms Universität, Bonn, Germany
Abstracts / Bone 45 (2009) S59–S111
PT-19 The serum concentration of the receptor activator of nuclear factor-kB ligand (RANKL), osteoprotegerin (OPG) and lipids profile in children with nephrotic syndrome M. Panczyk-Tomaszewskaa, H. Ziolkowskaa, D. Adamczuka, E. Górskab, A. Stelmaszczyk-Emmelb, M. Roszkowska-Blaima a Department of Pediatrics and Nephrology, Medical University of Warsaw, Poland, Warsaw, Poland b Department of Laboratory Diagnostics and Clinical Imuunology of Developmental Age, Medical University of Warsaw, Warsaw, Poland Selected data indicates that bone mineralisation and lipids metabolism may be related, but the exact mechanism remains unknown. Aim: The aim of the study was to assess the serum concentration of RANKL/OPG system and lipids metabolism parameters in children with different stages of nephrotic syndrome (NS). Material and methods: Twenty two children with NS in remission aged from 6.0 to 17 years mean age 10.9 ± 4.0; 12 treated with glucocorticoid and vitamin D (group I), 10 without treatment (group II) were involved into the study. The control group consisted of 16 healthy children, aged from 1.9 to 17.8 years, mean age 10.5 ± 5.0 years. In the study groups serum concentrations of OPG, RANKL, calcium, phosphorus, total cholesterol, HDL-cholesterol, LDL-cholesterol, triglycerides, albumin, creatinine, 25OH D3, and 1,25(OH)2D3 were measured. Results: The concentration of OPG in children with NS (groups I and II) was significantly lower compared to control group (from 0.8 to 4.9 median 1.65; from 0.5 to 3.7 median 1.40; from 1.1 to 4.9 median 3.05 respectively, p < 0.01). There were no significant differences between serum concentrations of RANKL in groups I, II and control group. In the study groups the significant negative correlation was found between concentrations of OPG and HDLcholesterol level (r = −0.63, p < 0.05). There was no significant correlation between OPG, RANKL and other studied parameters. Conclusion: Plasma OPG may be considered as a new biomarker of lipid abnormalities. doi:10.1016/j.bone.2009.04.121
PT-20 Osteocalcin, n-telopeptides and beta crosslaps in Spanish adolescents L. Gracia-Marcoa,b, G. Vicente-Rodrígueza,b, J. Valtueñac, J.P. Rey-Lópeza,b, E. DíazMartínezd, M.I. Mesanaa,b, K. Widhalme, J.R. Ruizf, M. González-Grossc,g, M.J. Castillog, L.A. Morenoa,b a GENUD: “Growth, Exercise, Nutrition and Development” Research Group, University of Zaragoza, Zaragoza, Spain b School of Health Sciences, Department of Physiotherapy and Nursing, University of Zaragoza, Zaragoza, Spain c Department of Health and Human Performance, Faculty of Physical Activity and Sport Sciences-INEF, Universidad Politécnica de Madrid, Madrid, Spain d Clinical Laboratory, Sport and Medicine Center, CSD, Madrid, Spain e Division of Clinical Nutrition and Prevention, Department of Pediatrics, Medical University of Vienna, Vienna, Austria f Unit for Preventive Nutrition, Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden g Institut für Ernährungs- und Lebensmittelwissenschaften, Humanernährung, Rheinische Friedrich-Wilhelms Universität, Bonn, Germany h Department of Physiology, School of Medicine, University of Granada, Granada, Spain Introduction and objectives: Osteocalcin, aminoterminal propeptide of type I procollagen (PINP) and beta crosslaps have been
described as markers of bone metabolism. Therefore, we aimed to describe the concentration of these markers during adolescence in male and female Spanish adolescents. Methods: Blood samples were obtained in 117 Spanish adolescents (53 males and 64 females), aged 12.5–17.5 years, within the framework of the HELENA study. Serum osteocalcin, PINP and beta crosslaps concentrations were determined by electrochemiluminescence immunoassay “ECLIA” by Elecsys 2010 analyzer. Subjects were categorized by age and Tanner stage, in four and three categories, respectively. One-way ANOVA was carried out to determine differences between age and/or Tanner groups. Independent Samples TTest was applied to determine gender differences. Results: PINP decreased with age in both males and females (p < 0.05). Males had significantly higher concentration of osteocalcin, PINP and beta crosslaps compared with females in all age categories (p < 0.05) except in the ranges 15–15.99 years and 16– 17.5 years (beta crosslaps and PINP, respectively). When the sample was categorized by Tanner stage (Table 1), osteocalcin, PINP and beta crosslap concentrations decreased with maturation in males, while only PINP decreased with maturation in females. Males had significantly higher concentration than females for beta crosslaps at all Tanner stages and osteocalcin and PINP for Tanner 5. Table 1 Bone markers concentration according to Tanner stage.
n TANNER
3 4 5
p
Male Osteocalcin (ng/mL)
PINP (ng/mL)
Beta crosslaps (ng/mL)
53 153.97 ± 46.47a 136.53 ± 54.94 100.54 ± 44.74⁎ 0.013
47 1185.9 ± 426.9a 878.35 ± 361.8b 501.9 ± 321.27⁎ 0.000
33 1.78 ± 0.18⁎ 2.09 ± 0.54b,⁎ 1.30 ± 0.44⁎ 0.002
Female Osteocalcin (ng/mL)
PINP (ng/mL)
Beta crosslaps (ng/mL)
64 99.48 ± 25.88 96.77 ± 43.03 58.44 ± 41.82 0.096
61 619.2 ± 21.9a 564.5 ± 359.5 259.2 ± 199.13 0.003
47 1.43 ± 0.19 0.98 ± 0.41 0.95 ± 0.59 0.394
⁎
p < 0.05 between gender; ap < 0.05 between Tanner 3 and 5; p < 0.05 between Tanner 4 and 5. Conclusions: Male adolescents showed higher concentrations of osteocalcin, PINP and beta crosslaps than females through adolescence. Bone turnover seems to decrease during adolescence, especially in males. b
doi:10.1016/j.bone.2009.04.122
PT-21 Bone markers and physical activity in Spanish adolescents L. Gracia-Marcoa,b, G. Vicente-Rodrígueza,b, J. Valtueñac, J.P. Rey-Lópeza,b, E. Díaz Martínezd, M.I. Mesanaa,b, K. Widhalme, J.R. Ruizf, M. González-Grossc,g, M. Sjöströmf, M.J. Castilloh, L.A. Morenoa,b a GENUD: “Growth, Exercise, Nutrition and Development” Research Group, University of Zaragoza, Zaragoza, Spain b School of Health Sciences, Department of Physiotherapy and Nursing, University of Zaragoza, Zaragoza, Spain c Department of Health and Human Performance, Faculty of Physical Activity and Sport Sciences-INEF, Universidad Politécnica de Madrid, Madrid, Spain d Clinical Laboratory, Sport and Medicine Center, CSD, Madrid, Spain e Division of Clinical Nutrition and Prevention, Department of Pediatrics, Medical University of Vienna, Vienna, Austria f Unit for Preventive Nutrition, Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden g Institut für Ernährungs- und Lebensmittelwissenschaften Humanernährung, Rheinische Friedrich-Wilhelms Universität, Bonn, Germany