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Other foodborne pathogens seen in western australia
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MICROBIOLOGY LISTERIA
IN QUEENSLAND
J. Bates, Laboratory of Microbiology and Pathology, Queensland Health, QLD. 4001.
OTHER FOODBORNE PATHOGENS SEEN
IN WESTERN AUSTRALIA A.K. 01T A N D E.. REED
The Queensland Health Department first became interested in Listerin s p p . in food following reports of outbreaks in Europe associated with the consumption o f soft cheese. Initially, samples submitted to the Public Health Microbiology laboratory for analysis reflected this concern and the majority of samples examined were from imported soft cheeses. In the following years, this testing was expanded considerably. both in the range and numbers of individual food types examined.
John Snow (1854) and William Budd (1856) recognized that drinking water could spread cholera and typhoid respectively, but it was only in 1888 that food-poisoning bacteria was first described by Gaertner.
Foods investigated for the presence of Lisleria spp. include cheese, milk, cream, ice-cream, precooked frozen meals, pate and manufactured meats. Data will be presented showing analyses of the various food types tested, along with the number of samples positive for each category. A discussion of the implications to be drawn from these results will be followed.
Since then, attempts to reduce the prevalence of foodborne diseases by legislation, improved public health facilities and increased resources for hygiene education have occurred. However, the incidence is increasing in most of the developed countries, particulary infections caused by the salmonella group and campylobacters..
Presenter: John Bates, Public Health Microbiology, Laboratory of Microbiology and Pathology.
The aetiological agents, the laboratory investigations of the more common pathogens viz: Salmonella spp, Campylobacter spp, Bacillus cereus, Staphylococcus spp and the Australian Food Standards are analysed.
proMICRO Pty Ltd, 31 Green Road, Hiliarys. Perth W.A. 6025.
The microbiological data from a production line of a smallgoods manufacturer, cooked-chiI led food and pre-prepared meals are used as illustrations of the sources of contamination of the foodlines, as well as the preventative measures implemented. Presenter : Dr. Keith 011, Consultant, proMICR0 Pty Ltd.
AN OUTBREAK OF LISTERIOSIS IN
WESTERN AUSTRALIA A.K. OlT AND J.I. WELLS
Department of Pathology, King Edward Memorial Hospital for Women, Subiaco. Perth. W.A. 6008.
In Western Australia, there were 14 cases of human listeriosis reported between June 1990 and September 1990. The majority of patients (9) were pregnant women, seven of whom were admitted to King Edward Memorial Hospital for Women.
The clinical details show that these women delivered three preterm livebirths (1 set of twins), three stillbirths, and there were two miscarriages. Standard laboratory methods for the isolation and identification of Lisreria monocyrogenes were performed, as well as for the epidemiological markers. Although no common source of the organism could be identified, the Health Department of W.A. had been aware of potential sources through the microbiological surveillance laboratory reports of smallgoods which were contaminated with Lisreria monocyrogenes. The Health Department publicized the potential risk of acquiring listeriosis through the electronic media and withdrew one product from the shelves of retail outlets, as well as issuing a short bulletin about listeriosis to all medical practitioners and other health professionals and informing smallgood manufacturers of hygiene standards. Presenter : Dr. Keith Ott, KEMH
THE INFECTIVITY OF LISTERIA MONOCYTOGENES FOllOWING INTRAGASTRIC INOCULATION INTO MICE. Angela Barbour, Anita Rampling* and C E Hormaeche, Public Health Laboratory, Dorchester and University of Cambridge, UK. Most studies of virulence of Listeria monocvtoaenes have used intravenous or intraperitoneal routes for inoculation of experimental animals or have used cell culture models. We used the oral route of infection because it is a more natural route for study of all aspects of the process including survival in the gut, invasion and systemic spread. We compared the infectivity for mice of 68 phenotypically normal, wild type isolates belonging to a range of serotypes and derived from clinical, food and environmental specimens. All isolates were first passaged in mice using i v inoculation. Balb/c mice, 8-12 weeks of age, were then inoculated with approximately 2 x lo9 log phase organisms. Counts of L.monocvtoqenes in the liver and spleen were performed 3 days post inoculation. Infectivity varied among isolates. Most isolates were invasive with 10'-106 CFU/liver and 103-10' CFU/spleen. A few isolates showed very low infectivity or were non-infective and some were intermediate. The least invasive isolates tended to belong to serotypes least commonly found in patients with clinical infection. Serotype 4b strains were more invasive as a group than other serotypes. Clinical isolates also showed a range of infectivity and were not always in the most infective group. Poorly invasive isolates survived less well in the gut than invasive isolates and were also less virulent following intravenous inoculation. Presenter: Or Anita Rampling, Laboratory, Dorchester, UK.
Public
Health
MICROBIOLOGY LISTERIA
IN QUEENSLAND
J. Bates, Laboratory of Microbiology and Pathology, Queensland Health, QLD. 4001.
OTHER FOODBORNE PATHOGENS SEEN
IN WESTERN AUSTRALIA A.K. 01T A N D E.. REED
The Queensland Health Department first became interested in Listerin s p p . in food following reports of outbreaks in Europe associated with the consumption o f soft cheese. Initially, samples submitted to the Public Health Microbiology laboratory for analysis reflected this concern and the majority of samples examined were from imported soft cheeses. In the following years, this testing was expanded considerably. both in the range and numbers of individual food types examined.
John Snow (1854) and William Budd (1856) recognized that drinking water could spread cholera and typhoid respectively, but it was only in 1888 that food-poisoning bacteria was first described by Gaertner.
Foods investigated for the presence of Lisleria spp. include cheese, milk, cream, ice-cream, precooked frozen meals, pate and manufactured meats. Data will be presented showing analyses of the various food types tested, along with the number of samples positive for each category. A discussion of the implications to be drawn from these results will be followed.
Since then, attempts to reduce the prevalence of foodborne diseases by legislation, improved public health facilities and increased resources for hygiene education have occurred. However, the incidence is increasing in most of the developed countries, particulary infections caused by the salmonella group and campylobacters..
Presenter: John Bates, Public Health Microbiology, Laboratory of Microbiology and Pathology.
The aetiological agents, the laboratory investigations of the more common pathogens viz: Salmonella spp, Campylobacter spp, Bacillus cereus, Staphylococcus spp and the Australian Food Standards are analysed.
proMICRO Pty Ltd, 31 Green Road, Hiliarys. Perth W.A. 6025.
The microbiological data from a production line of a smallgoods manufacturer, cooked-chiI led food and pre-prepared meals are used as illustrations of the sources of contamination of the foodlines, as well as the preventative measures implemented. Presenter : Dr. Keith 011, Consultant, proMICR0 Pty Ltd.
AN OUTBREAK OF LISTERIOSIS IN
WESTERN AUSTRALIA A.K. OlT AND J.I. WELLS
Department of Pathology, King Edward Memorial Hospital for Women, Subiaco. Perth. W.A. 6008.
In Western Australia, there were 14 cases of human listeriosis reported between June 1990 and September 1990. The majority of patients (9) were pregnant women, seven of whom were admitted to King Edward Memorial Hospital for Women.
The clinical details show that these women delivered three preterm livebirths (1 set of twins), three stillbirths, and there were two miscarriages. Standard laboratory methods for the isolation and identification of Lisreria monocyrogenes were performed, as well as for the epidemiological markers. Although no common source of the organism could be identified, the Health Department of W.A. had been aware of potential sources through the microbiological surveillance laboratory reports of smallgoods which were contaminated with Lisreria monocyrogenes. The Health Department publicized the potential risk of acquiring listeriosis through the electronic media and withdrew one product from the shelves of retail outlets, as well as issuing a short bulletin about listeriosis to all medical practitioners and other health professionals and informing smallgood manufacturers of hygiene standards. Presenter : Dr. Keith Ott, KEMH
THE INFECTIVITY OF LISTERIA MONOCYTOGENES FOllOWING INTRAGASTRIC INOCULATION INTO MICE. Angela Barbour, Anita Rampling* and C E Hormaeche, Public Health Laboratory, Dorchester and University of Cambridge, UK. Most studies of virulence of Listeria monocvtoaenes have used intravenous or intraperitoneal routes for inoculation of experimental animals or have used cell culture models. We used the oral route of infection because it is a more natural route for study of all aspects of the process including survival in the gut, invasion and systemic spread. We compared the infectivity for mice of 68 phenotypically normal, wild type isolates belonging to a range of serotypes and derived from clinical, food and environmental specimens. All isolates were first passaged in mice using i v inoculation. Balb/c mice, 8-12 weeks of age, were then inoculated with approximately 2 x lo9 log phase organisms. Counts of L.monocvtoqenes in the liver and spleen were performed 3 days post inoculation. Infectivity varied among isolates. Most isolates were invasive with 10'-106 CFU/liver and 103-10' CFU/spleen. A few isolates showed very low infectivity or were non-infective and some were intermediate. The least invasive isolates tended to belong to serotypes least commonly found in patients with clinical infection. Serotype 4b strains were more invasive as a group than other serotypes. Clinical isolates also showed a range of infectivity and were not always in the most infective group. Poorly invasive isolates survived less well in the gut than invasive isolates and were also less virulent following intravenous inoculation. Presenter: Or Anita Rampling, Laboratory, Dorchester, UK.
Public
Health