- Email: [email protected]
Over-exprssion of certain oncogenes in preneoplastic and neoplastic stages during rat hepatocarcinogenesis
1757
Meeting Abstracts
-m (Pm ~c!sa!6lLTIsr?!GEEIS INIiuWANURUlWELIAL~LINES
IN
N.Qm&nska,B.Christensen,J.Kielerand M.Sa&ur&a Fibiger Institute,Qqenhagen,Dstxnark;and Biochemistry, Medical Institute of Cracow,Pnlarid urothelial cell lines of Among 12 nonnalandtumxr (TCC)origin,representi.ng different transformationgrades (lGr) & vitro (I-III), a nuclear PK variant inhibitedby L-cysteine has beenfourdin the tunnxigenic!IGrIII cell lines only. Chranatin extracts of all cell lines containedthreePKisoenzymes whichshot+ed the greatestelectropixxetic mcbilityin !IGr 'IGr I cells. It diminished in imnortalized II and III cells,and in n;r III cells the slow migrating isoenzyme acquired sensitivityto I.-cysteine inhibition.Fran the T-24 Tee derived l'Gr III cell line a subline (T-24a) with reducedtumourigenic prqerties was isolated. 'Ihis sublins reduction of the shrxed a sirrplltaneous sensitivity of PK to L-cysteineinhibition. It is concluded that changes inPK isoenzymes might express multistage in vitro alterations durins --typic carcinogenesis. DIFEPiFNCEIN5'---AND SZvERALTwxlRcEU mPATI%RNE@IWEFN LINES WITH LCW- AND BIQI-M?&ICSnt?r mPExTlEs E.W.Hae.ffn~, K.-H.Doenges, J.wlcfiholz and B.Winkler Institut fiir 7&lDSUtSCheS
und lXmkxbiologie, Krebsforschungszentrum,
Heidelberg, F.R.G. Data on tbepl.asmam~&~anestxucture of severalexperimentaltumor cell lines with different tunxxrigenicandmetastatic propertieshasbeen obtained. These cell lines include the law-malignantmouse ascites HD33, themuselylra?hcmaEbandthe rat adenocarcinana BSp73 AS cells,and their high-malignant variants, mcuse ascitesI-ID34 and mouse 1yrrrphaM Bsb, and the rat adenocxcinana Bsp73 ASlL. The purified plaam ms&ZanS were investigateawith respect to their protein and lipid axnposition, and theirenzymeactivity. The mostpraninentdifferenceswere found for the specific, concanavalinA-inhibitable, 5'-nucleotidase activity, ark3 for the protein pattern exhibiting two to seven
t.imeShigkrenzym? acrivitiesandagreater annmt of slightlyacidic (basic)proteins preferentiallyinthelcwarmolecularweight rame (25 to 60 kD) of the low-malisnant celis. 'Ihesialica&d antent was f& to significantly higheZ in the be lcw-me&&asizing variants, andalsotbeir me&rane lipid fluidity was highercanpared tothehigh-metastasizingcell lines. Tlle data has beenevaluatedinrelationtothe malignantme.9 of thesetumxr cells.
rJvEa+~IoNQF~ ~CANDNSTSTI\E;ESDURING RATHEPA'IWAR~IS SJIaesen,V&&t(l)
am3GmEs
IN
and M.K.ixscb-Voiders
Antropogenetics, EYee Laboratory for University Brussels, Brussels,Belgiumand (1)rJniti de Biochim. TaKicol.et Cancerol., Univ.Cath.Louvain,Brussels,Belgium been over-expressed during hepatocarc~is in rats. Bowever,an interpretation of the sequentialanalysisof thesechangesinrelationtoothergenetical. and enzymaticalalterations, represents a newapproach. Weanalysedthesechangesin experimental model of Z&xcarcinogenesiswheremale Wistarr% were sutnitted to a tripbasic inducticm Glloyiation, selection,pmx+on). messenger-RNA fran rat liver, Ncathern blotting and hybridisationwith radioactively labelled CXC-prcbes,were carriedout. Adistinctionwasmadebetween nodular/non-Ixx%lar tissw m-RNA and tumxrigenic/Migenic tissuem-RNA. A first series of resultsis in aax&ance withpublisheddata:there is an elevated level of ras onrxqnetranscriptsfoundin regenerativXiver30 hcnrs after partial. hepatectany,inthencdulestisurrxlnding parenchymaandinboththetumourigenicand non-tunrxrigenictissue. The quantitative analysis, by densitunetry of the autoradiographics, is uriderinvestigation. LtEX'IN-BINDING ANDAEFE'INlTYcIWCf4A~ SEPARATI~m'IuKxlRcELLs B.Baqar, G.Burnsand L.J.Erkell MedicalFaculty, KuwaitUniversity,Kuwait, and Institutfiir Imnunolcgieund Genetik, Dsutsches Krebsforschungsinstitut, Heidelberg, F.R.G. Tumaur cell surface characteristics have been implicatedin severalaspectsof cancer metastasis. We have made quantitativeestimationsof sugar groups
Meeting Abstracts
-m (Pm ~c!sa!6lLTIsr?!GEEIS INIiuWANURUlWELIAL~LINES
IN
N.Qm&nska,B.Christensen,J.Kielerand M.Sa&ur&a Fibiger Institute,Qqenhagen,Dstxnark;and Biochemistry, Medical Institute of Cracow,Pnlarid urothelial cell lines of Among 12 nonnalandtumxr (TCC)origin,representi.ng different transformationgrades (lGr) & vitro (I-III), a nuclear PK variant inhibitedby L-cysteine has beenfourdin the tunnxigenic!IGrIII cell lines only. Chranatin extracts of all cell lines containedthreePKisoenzymes whichshot+ed the greatestelectropixxetic mcbilityin !IGr 'IGr I cells. It diminished in imnortalized II and III cells,and in n;r III cells the slow migrating isoenzyme acquired sensitivityto I.-cysteine inhibition.Fran the T-24 Tee derived l'Gr III cell line a subline (T-24a) with reducedtumourigenic prqerties was isolated. 'Ihis sublins reduction of the shrxed a sirrplltaneous sensitivity of PK to L-cysteineinhibition. It is concluded that changes inPK isoenzymes might express multistage in vitro alterations durins --typic carcinogenesis. DIFEPiFNCEIN5'---AND SZvERALTwxlRcEU mPATI%RNE@IWEFN LINES WITH LCW- AND BIQI-M?&ICSnt?r mPExTlEs E.W.Hae.ffn~, K.-H.Doenges, J.wlcfiholz and B.Winkler Institut fiir 7&lDSUtSCheS
und lXmkxbiologie, Krebsforschungszentrum,
Heidelberg, F.R.G. Data on tbepl.asmam~&~anestxucture of severalexperimentaltumor cell lines with different tunxxrigenicandmetastatic propertieshasbeen obtained. These cell lines include the law-malignantmouse ascites HD33, themuselylra?hcmaEbandthe rat adenocarcinana BSp73 AS cells,and their high-malignant variants, mcuse ascitesI-ID34 and mouse 1yrrrphaM Bsb, and the rat adenocxcinana Bsp73 ASlL. The purified plaam ms&ZanS were investigateawith respect to their protein and lipid axnposition, and theirenzymeactivity. The mostpraninentdifferenceswere found for the specific, concanavalinA-inhibitable, 5'-nucleotidase activity, ark3 for the protein pattern exhibiting two to seven
t.imeShigkrenzym? acrivitiesandagreater annmt of slightlyacidic (basic)proteins preferentiallyinthelcwarmolecularweight rame (25 to 60 kD) of the low-malisnant celis. 'Ihesialica&d antent was f& to significantly higheZ in the be lcw-me&&asizing variants, andalsotbeir me&rane lipid fluidity was highercanpared tothehigh-metastasizingcell lines. Tlle data has beenevaluatedinrelationtothe malignantme.9 of thesetumxr cells.
rJvEa+~IoNQF~ ~CANDNSTSTI\E;ESDURING RATHEPA'IWAR~IS SJIaesen,V&&t(l)
am3GmEs
IN
and M.K.ixscb-Voiders
Antropogenetics, EYee Laboratory for University Brussels, Brussels,Belgiumand (1)rJniti de Biochim. TaKicol.et Cancerol., Univ.Cath.Louvain,Brussels,Belgium been over-expressed during hepatocarc~is in rats. Bowever,an interpretation of the sequentialanalysisof thesechangesinrelationtoothergenetical. and enzymaticalalterations, represents a newapproach. Weanalysedthesechangesin experimental model of Z&xcarcinogenesiswheremale Wistarr% were sutnitted to a tripbasic inducticm Glloyiation, selection,pmx+on). messenger-RNA fran rat liver, Ncathern blotting and hybridisationwith radioactively labelled CXC-prcbes,were carriedout. Adistinctionwasmadebetween nodular/non-Ixx%lar tissw m-RNA and tumxrigenic/Migenic tissuem-RNA. A first series of resultsis in aax&ance withpublisheddata:there is an elevated level of ras onrxqnetranscriptsfoundin regenerativXiver30 hcnrs after partial. hepatectany,inthencdulestisurrxlnding parenchymaandinboththetumourigenicand non-tunrxrigenictissue. The quantitative analysis, by densitunetry of the autoradiographics, is uriderinvestigation. LtEX'IN-BINDING ANDAEFE'INlTYcIWCf4A~ SEPARATI~m'IuKxlRcELLs B.Baqar, G.Burnsand L.J.Erkell MedicalFaculty, KuwaitUniversity,Kuwait, and Institutfiir Imnunolcgieund Genetik, Dsutsches Krebsforschungsinstitut, Heidelberg, F.R.G. Tumaur cell surface characteristics have been implicatedin severalaspectsof cancer metastasis. We have made quantitativeestimationsof sugar groups