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Overactive bladder is associated with channelopathy of potassium channels
4th Baltic Meeting in conjunction with the EAU, 26-27 May 2017, Vilnius, Lithuania
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Overactive bladder is associated with channelopathy of potassium channels Eur Urol Suppl 2017; 16(5);e2222
Iatsyna O.1, Sapsai A.2, Parshikov O.3, Soloviev A.3, Kostev F.4, Yalovenko K.5 1
National Institution of Urology, Dept. of Neurourology, Kyiv, Ukraine, 2Mozmbique Militar Hospital, Dept. of Urology, Kyiv, Ukraine, 3State Institution "Institute of Pharmacology and Toxicology of The National Academy of Medical Scien, Dept. of Experimental Therapy, Kyiv, Ukraine, 4Odessa State Medical University, Dept. of Urology, Odessa, Ukraine, 5Week-End Clinic, Dept. of Gynaecology, Kyiv, Ukraine INTRODUCTION & OBJECTIVES: Overactive bladder disease is characterized with nocturia, frequency, urgency, often with incontinencethat worsening the comfort of patients life. Nowadays it is known that overactive bladder associated with a malfunction of neurons, smooth muscle and urothelium, but the mechanism of this dysfunction remains unclear. However, it probably might be due to ВКСа channelopathy in bladder smooth muscle. MATERIAL & METHODS: Using the method of patch clamp we compare the characteristics of potassium current in smooth muscle cells of healthy Wistar line rats and rats with an overactive bladder model, clarify the role of ВКСа channels in the bladder and propose the pharmacologicalcorrection of these channels by liposomal quercetin. RESULTS: We have shown a significant decreasing of the potassium current density in smooth muscle cells of the rats with overactive bladder, from 168.7 ± 5.8 pA/pF in control to 65.7 ± 12.3 pA/pF in the model. Selective blocker of ВКСа channels paxilline reduced potassium current about half, indicating significant functional expression of these channels and their important role in the regulation of bladder contractile activity. An perspective activator of ВКСа channels, liposomalquercetin, increased current density in model rats from 32.7 ± 0.9 pA/pF to 53.1 ± 2.7 pA/pF. CONCLUSIONS: Overactive bladder disease is associated with channelopathy of potassium channels, particularly with ВКСа channels and their suppressed function can be partially restored by liposomal quercetin.
Eur Urol Suppl 2017; 16(5);e2222 Powered by TCPDF (www.tcpdf.org)
52
Overactive bladder is associated with channelopathy of potassium channels Eur Urol Suppl 2017; 16(5);e2222
Iatsyna O.1, Sapsai A.2, Parshikov O.3, Soloviev A.3, Kostev F.4, Yalovenko K.5 1
National Institution of Urology, Dept. of Neurourology, Kyiv, Ukraine, 2Mozmbique Militar Hospital, Dept. of Urology, Kyiv, Ukraine, 3State Institution "Institute of Pharmacology and Toxicology of The National Academy of Medical Scien, Dept. of Experimental Therapy, Kyiv, Ukraine, 4Odessa State Medical University, Dept. of Urology, Odessa, Ukraine, 5Week-End Clinic, Dept. of Gynaecology, Kyiv, Ukraine INTRODUCTION & OBJECTIVES: Overactive bladder disease is characterized with nocturia, frequency, urgency, often with incontinencethat worsening the comfort of patients life. Nowadays it is known that overactive bladder associated with a malfunction of neurons, smooth muscle and urothelium, but the mechanism of this dysfunction remains unclear. However, it probably might be due to ВКСа channelopathy in bladder smooth muscle. MATERIAL & METHODS: Using the method of patch clamp we compare the characteristics of potassium current in smooth muscle cells of healthy Wistar line rats and rats with an overactive bladder model, clarify the role of ВКСа channels in the bladder and propose the pharmacologicalcorrection of these channels by liposomal quercetin. RESULTS: We have shown a significant decreasing of the potassium current density in smooth muscle cells of the rats with overactive bladder, from 168.7 ± 5.8 pA/pF in control to 65.7 ± 12.3 pA/pF in the model. Selective blocker of ВКСа channels paxilline reduced potassium current about half, indicating significant functional expression of these channels and their important role in the regulation of bladder contractile activity. An perspective activator of ВКСа channels, liposomalquercetin, increased current density in model rats from 32.7 ± 0.9 pA/pF to 53.1 ± 2.7 pA/pF. CONCLUSIONS: Overactive bladder disease is associated with channelopathy of potassium channels, particularly with ВКСа channels and their suppressed function can be partially restored by liposomal quercetin.
Eur Urol Suppl 2017; 16(5);e2222 Powered by TCPDF (www.tcpdf.org)