- Email: [email protected]
Overexpression of Claudin-1 Is Associated with Poor Prognosis of Oral Squamous Cell Carcinoma
OOOO Volume 119, Number 3 clinical symptoms and signs in patients with ulcerative oral lichen planus (OLP). Study Design: Serum levels of IL-17, pain and burning sensation severity based on VAS and the maximum size of the lesions of 25 patients with ulcerative oral lichen planus were determined before and after administration of propolis for 30 days (a 500-mg capsule daily). Data was analyzed by KolmogorovSmirnov test and t-test using SPSS 13 (p<0.05). Results: IL-17 levels, pain and burning sensation severities and lesion sizes before and after intervention were 13516.5, 127 12 pg/mL (p¼0.006), 4.23.6, 3.3 2.8 (p¼0.049) and 14 7.7, 9.8 7.6 mm, respectively (p¼0.03). Conclusions: Administration of propolis significantly decreased IL-17 serum levels, VAS means and the maximum lesion sizes in patients with ulcerative lichen planus. Keywords: ulcerative oral lichen planus, Interleukin 17, propolis
EFFECT OF MUCOADHESIVE FORM OF MYCOPHENOLATE MOFETIL ON ORAL EROSIVE LICHEN PLANUS LESIONS Ali Taghavi Zenuz1, Masoumeh Mehdipour1, Javad Shokri2, Negin Samiei1, Marziyeh Aghazadeh1, 1 Department of Oral medicine, Tabriz University of Medical Sciences; 2Department of Pharmaceutics, Tabriz University of Medical Sciences, Tabriz, Iran Objective: This study was performed to determine the effect of 2% Mycophenolate mofetil (MMF) mucoadhesive on ulcer size and severity of burning sensation and pain in oral lichen planus (OLP) patients using visual analog scale (VAS). Study Design: VAS and lesion sizes of 27 patients with OLP (group A: 10 patients with bilateral ulcerative lesions, a double-blind clinical trial with mucoadhesive and placebo, group B: 17 patients with unilateral ulcerative lesions, a before-after clinical trial) were recorded at baseline and each week of the treatment for 4 weeks. Data were analyzed with paired t-test using SPSS 16. Results: After 4 weeks there were significant reductions in VAS severity in groups A (P¼0.012,) and B (P¼0.004). The lesion sizes decreased on case sides in groups A (P¼0.004) and B (P¼0.002). Conclusions: MMF mucoadhesive was effective in decreasing VAS severity and ulcer sizes in ulcerative OLP. Keywords: Mycophenolate Mofetil, Oral Erosive Lichen Planus Lesions
CELL DEATH AND RESISTANCE BY HSPS IN PDTTREATED CANCER CELLS Jisun Kim, Sanwoo Kim, Hongran Choi, Okjoon Kim, Department of oral pathology, Dental Science Research Institute and, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea Objective: Photodynamic therapy (PDT) is an effective cancer therapy through by irradiation after specific photosensitizer. However, PDT shows the resistance by the alteration of gene expression and protein activity. Heat shock protein (HSP) has been suggested to play an important role in the regulation of cell death and PDT-resistance. Study Design: Fadu cells were treated with hematophrphyrin, followed by irradiation. We investigated the alteration of HSPs expression. We checked the effects of PDTinduced cell death in down-regulation of HSPs.
ABSTRACTS Abstracts e167 Results: HSP27 expression was decreased by in Fadu cells. In contrast, HSP70 and HSP90 expressions were increased by PDT. HSP70 and 90 were regulated by LC3II. However, HSP27 was regulated to PARP-1. HSP27 and Hsp70 have roles of crosstalk which regulated cell death through by autophagy and apoptosis. Conclusions: Therefore, HSP27 and HSP70 were necessary to increase the PDT effects against PDT resistance which was happened by compensation of autophagic and apoptotic cell death. Keywords: Heat shock protein, photodynamic terapy, cancer cells
OVEREXPRESSION OF CLAUDIN-1 IS ASSOCIATED WITH POOR PROGNOSIS OF ORAL SQUAMOUS CELL CARCINOMA Ekarat Phattarataratip1, Kraisorn Sappayatosok2, 1Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand; 2Faculty of Dental Medicine, Rangsit University, Bangkok, Thailand Objective: Claudins constitute a group of proteins forming the principal element of tight junctions. The altered expressions of several claudins have been reported in cancer cells. The purpose of this study was to investigate the expressions of claudin-1 and claudin-4 in oral squamous cell carcinoma and examine their relationships with patient clinical-pathologic features. Study Design: 45 oral squamous cell carcinoma cases were enrolled and the claudin-1 and claudin-4 expressions were determined immunohistochemically. Results: The claudin-1 and claudin-4 immunoreactivities were noted in 86.7% and 80% of cases, respectively. The majority of cases showed the positive staining in <25% of cancer cells. The increased claudin-1 expression was significantly associated with high pathologic grade, nodal metastasis and the advanced clinical stage. No relationship with different claudin-4 expressions was observed. Conclusions: Claudin-1 may play a role in oral squamous cell carcinoma progression and could serve as a prognostic indication of this neoplasm. Keywords: Claudin-1, Claudin-4, Oral squamous cell carcinoma
MYOEPITHELIOMA OF THE BUCCAL MUCOSA WITH A PSEUDO-CRIBRIFORM PATTERN: REPORT OF A RARE CASE AND REVIEW OF THE LITERATURE Maryam Khalili1, Fereshteh Baghai1, Arghavan Etebarian1, Farzad Yazdani2, 1Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences; 2 Department of Pathology, Tehran University of Medical Sciences, Tehran, Iran Myoepithelioma is a rare neoplasm of the salivary glands, represents 1.5% of all salivary gland tumors. It is localized most often in parotid and to a lesser extent in the minor intraoral salivary glands. It was considered to be a type of pleomorphic adenoma but today myoepitheliomas are believed to be a distinct pathological entity which must be differentiated from several benign and malignant epithelial and mesenchymal tumors. Conservative surgery is the treatment of choice and apparently presents a good prognosis. Myoepithelioma of the buccal mucosa is very rare and only five cases have been reported in the world literature so far. Here, we present another case of myoepithelioma of the minor salivary glands of the buccal area with emphasis on
EFFECT OF MUCOADHESIVE FORM OF MYCOPHENOLATE MOFETIL ON ORAL EROSIVE LICHEN PLANUS LESIONS Ali Taghavi Zenuz1, Masoumeh Mehdipour1, Javad Shokri2, Negin Samiei1, Marziyeh Aghazadeh1, 1 Department of Oral medicine, Tabriz University of Medical Sciences; 2Department of Pharmaceutics, Tabriz University of Medical Sciences, Tabriz, Iran Objective: This study was performed to determine the effect of 2% Mycophenolate mofetil (MMF) mucoadhesive on ulcer size and severity of burning sensation and pain in oral lichen planus (OLP) patients using visual analog scale (VAS). Study Design: VAS and lesion sizes of 27 patients with OLP (group A: 10 patients with bilateral ulcerative lesions, a double-blind clinical trial with mucoadhesive and placebo, group B: 17 patients with unilateral ulcerative lesions, a before-after clinical trial) were recorded at baseline and each week of the treatment for 4 weeks. Data were analyzed with paired t-test using SPSS 16. Results: After 4 weeks there were significant reductions in VAS severity in groups A (P¼0.012,) and B (P¼0.004). The lesion sizes decreased on case sides in groups A (P¼0.004) and B (P¼0.002). Conclusions: MMF mucoadhesive was effective in decreasing VAS severity and ulcer sizes in ulcerative OLP. Keywords: Mycophenolate Mofetil, Oral Erosive Lichen Planus Lesions
CELL DEATH AND RESISTANCE BY HSPS IN PDTTREATED CANCER CELLS Jisun Kim, Sanwoo Kim, Hongran Choi, Okjoon Kim, Department of oral pathology, Dental Science Research Institute and, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea Objective: Photodynamic therapy (PDT) is an effective cancer therapy through by irradiation after specific photosensitizer. However, PDT shows the resistance by the alteration of gene expression and protein activity. Heat shock protein (HSP) has been suggested to play an important role in the regulation of cell death and PDT-resistance. Study Design: Fadu cells were treated with hematophrphyrin, followed by irradiation. We investigated the alteration of HSPs expression. We checked the effects of PDTinduced cell death in down-regulation of HSPs.
ABSTRACTS Abstracts e167 Results: HSP27 expression was decreased by in Fadu cells. In contrast, HSP70 and HSP90 expressions were increased by PDT. HSP70 and 90 were regulated by LC3II. However, HSP27 was regulated to PARP-1. HSP27 and Hsp70 have roles of crosstalk which regulated cell death through by autophagy and apoptosis. Conclusions: Therefore, HSP27 and HSP70 were necessary to increase the PDT effects against PDT resistance which was happened by compensation of autophagic and apoptotic cell death. Keywords: Heat shock protein, photodynamic terapy, cancer cells
OVEREXPRESSION OF CLAUDIN-1 IS ASSOCIATED WITH POOR PROGNOSIS OF ORAL SQUAMOUS CELL CARCINOMA Ekarat Phattarataratip1, Kraisorn Sappayatosok2, 1Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand; 2Faculty of Dental Medicine, Rangsit University, Bangkok, Thailand Objective: Claudins constitute a group of proteins forming the principal element of tight junctions. The altered expressions of several claudins have been reported in cancer cells. The purpose of this study was to investigate the expressions of claudin-1 and claudin-4 in oral squamous cell carcinoma and examine their relationships with patient clinical-pathologic features. Study Design: 45 oral squamous cell carcinoma cases were enrolled and the claudin-1 and claudin-4 expressions were determined immunohistochemically. Results: The claudin-1 and claudin-4 immunoreactivities were noted in 86.7% and 80% of cases, respectively. The majority of cases showed the positive staining in <25% of cancer cells. The increased claudin-1 expression was significantly associated with high pathologic grade, nodal metastasis and the advanced clinical stage. No relationship with different claudin-4 expressions was observed. Conclusions: Claudin-1 may play a role in oral squamous cell carcinoma progression and could serve as a prognostic indication of this neoplasm. Keywords: Claudin-1, Claudin-4, Oral squamous cell carcinoma
MYOEPITHELIOMA OF THE BUCCAL MUCOSA WITH A PSEUDO-CRIBRIFORM PATTERN: REPORT OF A RARE CASE AND REVIEW OF THE LITERATURE Maryam Khalili1, Fereshteh Baghai1, Arghavan Etebarian1, Farzad Yazdani2, 1Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences; 2 Department of Pathology, Tehran University of Medical Sciences, Tehran, Iran Myoepithelioma is a rare neoplasm of the salivary glands, represents 1.5% of all salivary gland tumors. It is localized most often in parotid and to a lesser extent in the minor intraoral salivary glands. It was considered to be a type of pleomorphic adenoma but today myoepitheliomas are believed to be a distinct pathological entity which must be differentiated from several benign and malignant epithelial and mesenchymal tumors. Conservative surgery is the treatment of choice and apparently presents a good prognosis. Myoepithelioma of the buccal mucosa is very rare and only five cases have been reported in the world literature so far. Here, we present another case of myoepithelioma of the minor salivary glands of the buccal area with emphasis on