- Email: [email protected]
Oxidative stress in families of type 1 diabetic patients
S308
Poster Session 2
ily referred to an inhibition of cyclic prostanoids synthesis, however the other mechanisms have been proposed. On the other hand, the crucial role of platelets in the pathophysiology of vascular complications is connected not only with an augmented aggregation and release reaction. Platelets also take part in haemostasis cascade by the formation of catalytic surface for prothrombinase complex. This procoagulant activity depends on the amount of platelet factor 3 (PF3) contained in platelet membrane and small microparticles released from activated platelets. Aim: Therefore the aim of our study was to measure PF3 activity in platelet rich plasma (PRP), platelet poor plasma (PPP) and filtrated plasma (PFP) of diabetic patients, and to estimate the possible influence of ASA on PF3 availability in viva. Material and methods: The group studied consisted of 53 type 2 diabetic patients, divided into 2 groups: 37 persons (mean age 56.3 f 10.2 YS.) who did not take any antiplatelet drugs and 16 patients (mean age 63.0 f 5.65 YS.) given aspirin (75 - 150 mg/d) for at least 4 weeks, and 31 control subjects (mean age 38.9 +I 11.5 YS.). The activity of PF3 was measured using Russell’s viper venom (RVV, Sigma Chemicals), according to Wenche et al. PF3 availability in PFP reflected the amount and procoagulant activity of the small microparticles (i 1.0 km) released from platelet membranes during activation. ANOVA and the Mann Whitney U test were used for statistical analysis. Results: PF3 availability was significantly increased in diabetics in comparison with the controls, and did not differ between the patients taking and not taking ASA (6.79 f 0.96 &nl vs 6.08 f 1.01 &ml for PM, 3.05 f 0.45 &ml vs 3.31 f 0.64 Kg/ml for PPP and 0.93 f 0.12 &ml vs 0.98 rt 0.22 fig/ml for PFP). The highest differences between diabetics and healthy subjects were found in PPP (p
P1244 Oxidative Stress in Families of ‘Qye 1 Diabetic Patients E. MATTEUCCI, V. Cinapri, S. Quilici, G. Forotti, 0. Giampietro. Dipartimento di Medicina Interna, Universitic di Piss, Italy
The link between hyperglycemia and complications of diabetes mellitus is unknown. It is still discussed whether oxidative stress precedes or merely reflects diabetic complications. To search for a familial predisposition to oxidative stress, we investigated indices of glucose and lipid metabolism, markers of plasma and cell lipid oxidation, a marker of oxidant-induced protein damage, and the effects of oxygen radicals on erythrocytes of patients with type 1 diabetes and their relatives. Were recruited 30 type 1 diabetics (10 without diabetic complications, 10 with retinopathy, 10 with nephropathy), 36 non-diabetic siblings, 37 non-diabetic parents of type 1 diabetics, and 3 control groups of healthy subjects without family history of diabetes. Were determined blood creatinine, glucose, HbAlc, cholesterol, triglycerides, Lp(a), fibrinogen, malondialdehyde (MDA), advanced oxidation protein products (AOPP). Erythrocyte (RBC) response to oxidative stress (3-h-incubation at 37°C with or without a radical generating system) was evaluated by measuring erythrocyte glutathione (RBC GSH), RBC MDA, and haemolysis. Diabetic patients had elevated levels of blood glucose (p
while a significant increase in haemolysis. Among relatives, haemolysis was increased both at baseline and after incubation. Plasma MDA was associated with blood glucose, creatinine, and fibrinogen (multiple R 0.5, p
P1245 SerumLipoprotein(a) as a Risk Factor for Coronary Heart Disease in Kuwaiti ‘Qpe 2 Diabetic Patients N.A. ABDELLA’, O.A. Mojiminiyi’,A.O.
Akanji2, M.A. Moussa3.
’ Department
of Medicine, Faculty of Medicine Kuwait University, Kuwait; ‘Department of Pathology, Faculty of Medicine Kuwait University, Kuwait; 3 Department of Community Medicine, Faculty of Medicine Kuwait University, Kuwait
Serum lipoprotein(a) [Lp(a)], a risk factor for coronary heart disease (CHD) in nondiabetic populations, is largely under genetic control and varies among ethnic and racial groups. We evaluated Lp(a) [measured with an enzyme immunoassay (Biopool TintElize)] and traditional CHD risk factors (age, sex, body mass index (BMI), smoking, hypertension, dyslipidemia) as well as stage of diabetic nephropathy in 345 type 2 diabetic patients. Lp(a) concentration was skewed with median (2.5”. 97.5” percentile) of 25.0 (8.1, 75.7) mg/dl. 42% of patients with CHD had increased (>30mg/dl) Lp(a) compared with 35.2% of patients without CHD (p = 0.4). 55.6% of female patients with CHD had increased Lp(a) compared to 39.3% of males. Lp(a) was significantly (pc 0.05) higher in females than males. Although female patients with CHD and macroalbuminuria had significantly (p 10.05) higher Lp(a) than normoalbuminuric female patients without CHD, no such association was found in males and no significant association was found between Lp(a) and the degree of albuminuria. Partial correlation analysis controlling for age, sex and BMI showed significant correlation of Lp(a) with total cholesterol only (p = 0.03) and no correlation was found with other lipid parameters. Multiple regression analysis did not show significant associations of Lp(a) with standard CHD risk factors, WA,, and plasma creatinine. This study is in agreement with studies in other populations which showed a lack of association between Lp(a), CHD and DM. We conclude that Lp(a) is not an independent risk factor for CHD in Kuwaiti Arabs with type 2 DM.
P1246 Homocysteine and Its Association with Diabetic Complications in Kuwaiti Arab Subjects N.A. ABDELLA’, O.A. Mojiminiyi’, A.O. Akanji’, M.A. Moussa3.
‘Department of Medicine, Faculty of Medicine Kuwait University Kuwait; 2 Department of Pathology, Faculty of Medicine Kuwait University, Kuwait; 3 Department of Community Medicine, Faculty of Medicine Kuwait University, Kuwait This study investigates the association of hyperhomocysteinemia with coronary heart disease (CHD) and diabetic complications in 156 men and 202 women with Type 2 diabetes. Fastingplasma total homocysteine(tHcy) [AbottIMx], glycatedhaemoglobin (HbAI,) and urine albumin and creatinine (Bayer DCA 2000) were mea-
Poster Session 2
ily referred to an inhibition of cyclic prostanoids synthesis, however the other mechanisms have been proposed. On the other hand, the crucial role of platelets in the pathophysiology of vascular complications is connected not only with an augmented aggregation and release reaction. Platelets also take part in haemostasis cascade by the formation of catalytic surface for prothrombinase complex. This procoagulant activity depends on the amount of platelet factor 3 (PF3) contained in platelet membrane and small microparticles released from activated platelets. Aim: Therefore the aim of our study was to measure PF3 activity in platelet rich plasma (PRP), platelet poor plasma (PPP) and filtrated plasma (PFP) of diabetic patients, and to estimate the possible influence of ASA on PF3 availability in viva. Material and methods: The group studied consisted of 53 type 2 diabetic patients, divided into 2 groups: 37 persons (mean age 56.3 f 10.2 YS.) who did not take any antiplatelet drugs and 16 patients (mean age 63.0 f 5.65 YS.) given aspirin (75 - 150 mg/d) for at least 4 weeks, and 31 control subjects (mean age 38.9 +I 11.5 YS.). The activity of PF3 was measured using Russell’s viper venom (RVV, Sigma Chemicals), according to Wenche et al. PF3 availability in PFP reflected the amount and procoagulant activity of the small microparticles (i 1.0 km) released from platelet membranes during activation. ANOVA and the Mann Whitney U test were used for statistical analysis. Results: PF3 availability was significantly increased in diabetics in comparison with the controls, and did not differ between the patients taking and not taking ASA (6.79 f 0.96 &nl vs 6.08 f 1.01 &ml for PM, 3.05 f 0.45 &ml vs 3.31 f 0.64 Kg/ml for PPP and 0.93 f 0.12 &ml vs 0.98 rt 0.22 fig/ml for PFP). The highest differences between diabetics and healthy subjects were found in PPP (p
P1244 Oxidative Stress in Families of ‘Qye 1 Diabetic Patients E. MATTEUCCI, V. Cinapri, S. Quilici, G. Forotti, 0. Giampietro. Dipartimento di Medicina Interna, Universitic di Piss, Italy
The link between hyperglycemia and complications of diabetes mellitus is unknown. It is still discussed whether oxidative stress precedes or merely reflects diabetic complications. To search for a familial predisposition to oxidative stress, we investigated indices of glucose and lipid metabolism, markers of plasma and cell lipid oxidation, a marker of oxidant-induced protein damage, and the effects of oxygen radicals on erythrocytes of patients with type 1 diabetes and their relatives. Were recruited 30 type 1 diabetics (10 without diabetic complications, 10 with retinopathy, 10 with nephropathy), 36 non-diabetic siblings, 37 non-diabetic parents of type 1 diabetics, and 3 control groups of healthy subjects without family history of diabetes. Were determined blood creatinine, glucose, HbAlc, cholesterol, triglycerides, Lp(a), fibrinogen, malondialdehyde (MDA), advanced oxidation protein products (AOPP). Erythrocyte (RBC) response to oxidative stress (3-h-incubation at 37°C with or without a radical generating system) was evaluated by measuring erythrocyte glutathione (RBC GSH), RBC MDA, and haemolysis. Diabetic patients had elevated levels of blood glucose (p
while a significant increase in haemolysis. Among relatives, haemolysis was increased both at baseline and after incubation. Plasma MDA was associated with blood glucose, creatinine, and fibrinogen (multiple R 0.5, p
P1245 SerumLipoprotein(a) as a Risk Factor for Coronary Heart Disease in Kuwaiti ‘Qpe 2 Diabetic Patients N.A. ABDELLA’, O.A. Mojiminiyi’,A.O.
Akanji2, M.A. Moussa3.
’ Department
of Medicine, Faculty of Medicine Kuwait University, Kuwait; ‘Department of Pathology, Faculty of Medicine Kuwait University, Kuwait; 3 Department of Community Medicine, Faculty of Medicine Kuwait University, Kuwait
Serum lipoprotein(a) [Lp(a)], a risk factor for coronary heart disease (CHD) in nondiabetic populations, is largely under genetic control and varies among ethnic and racial groups. We evaluated Lp(a) [measured with an enzyme immunoassay (Biopool TintElize)] and traditional CHD risk factors (age, sex, body mass index (BMI), smoking, hypertension, dyslipidemia) as well as stage of diabetic nephropathy in 345 type 2 diabetic patients. Lp(a) concentration was skewed with median (2.5”. 97.5” percentile) of 25.0 (8.1, 75.7) mg/dl. 42% of patients with CHD had increased (>30mg/dl) Lp(a) compared with 35.2% of patients without CHD (p = 0.4). 55.6% of female patients with CHD had increased Lp(a) compared to 39.3% of males. Lp(a) was significantly (pc 0.05) higher in females than males. Although female patients with CHD and macroalbuminuria had significantly (p 10.05) higher Lp(a) than normoalbuminuric female patients without CHD, no such association was found in males and no significant association was found between Lp(a) and the degree of albuminuria. Partial correlation analysis controlling for age, sex and BMI showed significant correlation of Lp(a) with total cholesterol only (p = 0.03) and no correlation was found with other lipid parameters. Multiple regression analysis did not show significant associations of Lp(a) with standard CHD risk factors, WA,, and plasma creatinine. This study is in agreement with studies in other populations which showed a lack of association between Lp(a), CHD and DM. We conclude that Lp(a) is not an independent risk factor for CHD in Kuwaiti Arabs with type 2 DM.
P1246 Homocysteine and Its Association with Diabetic Complications in Kuwaiti Arab Subjects N.A. ABDELLA’, O.A. Mojiminiyi’, A.O. Akanji’, M.A. Moussa3.
‘Department of Medicine, Faculty of Medicine Kuwait University Kuwait; 2 Department of Pathology, Faculty of Medicine Kuwait University, Kuwait; 3 Department of Community Medicine, Faculty of Medicine Kuwait University, Kuwait This study investigates the association of hyperhomocysteinemia with coronary heart disease (CHD) and diabetic complications in 156 men and 202 women with Type 2 diabetes. Fastingplasma total homocysteine(tHcy) [AbottIMx], glycatedhaemoglobin (HbAI,) and urine albumin and creatinine (Bayer DCA 2000) were mea-