- Email: [email protected]
OXIDIZED LDL AND POSTMENOPAUSAL OBESITY
Poster Sessions PO31 Thrombogenic risk factors were 1.7 [1.0-2.7] vs. 0.8 [0.5-1.2] for men and 3.0 [1.9-4.9] vs. 0.4 [2.2-0.8] for women, corresponding to a mean hip-circumference of 91.0 cm vs. 107.0 cm for men and 89.0 cm vs. 110.0 cm for women. For both genders a threshold effect for low hip-circumference was evident around 100 cm, particularly for CVD morbidity. Conclusion: Relatively narrower hips seem to increase the risk for early cardiovascular morbidity and death compared to those with wider hips. Lack of muscle, fat, bone or all tissues may be responsible. Protection from wide hip circumference was greater for women than for men. A threshold effect seemed present for both gender, suggesting a critically too low hip circumference around 100 cm. Our data suggest that there may be no additional benefit to having wide hips above this threshold. PO30-514
RELATIVELY HIGH LEVELS OF SERUM ADIPONECTIN IN OBESE INDIVIDUALS, A MARKER OF ADIPONECTIN RESISTANCE: ROLE OF SEX HORMONE-BINDING GLOBULIN
Objectives: It is unclear whether serum adiponectin concentrations diminish linearly with increasing adiposity and which factors are codeterminants of this association. These issues were investigated cross-sectionally in a general population sample representative of middle-aged and elderly Turkish adults. Methods: In 1199 men and women, serum total adiponectin was assayed by ELISA. ‘Adiponectin adequacy’ was defined as serum concentrations of >8.3 in men, >10.6 µg/ml in women, corresponding to 45th percentile in either gender. Results: Serum adiponectin values in men, though declining significantly in transition from the bottom to the mid tertile of body mass index (BMI), waist circumference (WC) and hip circumference (HC), were similar in the two respective upper tertiles. In women, adiponectin was not significantly different in any tertile of these indices. Serum adiponectin significantly correlated with BMI, WC, or HC within the bottom tertiles and the top tertile of female HC but, otherwise, not within the upper two tertiles. In multiple logistic regression analyses for the likelihood of ‘adiponectin adequacy’ in two models, HDL-cholesterol and CRP were significantly associated in men, whereas in women SHBG [at significant ORs 1.5 for a 2-fold increment) and triglycerides were associated, independent of (abdominal) obesity markers and fasting insulin levels. Conclusions: Serum adiponectin is affected by SHBG independently from obesity and hyperinsulinemia, especially in obese women, which induces ‘adiponectin resistance’ and accounts for an inconsistent relationship between excess adiposity and adiponectin levels. Gender determines partial independence of anti-inflammatory properties of adiponectin from obesity and insulin resistance.
Results: A total of 1184 non-diabetic subjects (aged 49.6±8.1) were included. Mean IMT in both CCA (µm) and ICA (µm) was greater at baseline in overweight and obese subjects (defined by BMI). In multiple models, we found that both baseline and follow-up CCA-IMT were associated with overweight (β=20.9, p=0.01, and β=36.3, p<0.001, respectively) and obesity (β=24.4, p=0.008, and β=34.5, p=0.002, respectively). Regarding waist circumference, only CCA-IMT at baseline was associated with the third (β=25.2, p=0.005) and fourth quartiles (β=20.8, p=0.026). Conclusions: Our study showed a higher IMT of both CCA and ICA in overweight and obese subjects. BMI and waist circumference were independently associated with the IMT of CCA at baseline, whereas BMI predicted CCA-IMT during follow-up. These results suggest that central fat accumulation may accelerate the development of earlier clinically stages of atherosclerosis. PO30-516
E. Kassi, M. Dalamaga*, E. Faviou*, G. Hroussalas, K. Kazanis, A. Dionyssiou-Asteriou. Department of Biological Chemistry and Department of Clinical Biochemistry, Medical School, University of Athens, Athens, Greece Aim: To determine serum levels of oxidized LDL (oxLDL) and antibodies against them (anti-oxLDL) in postmenopausal women and evaluate their association with BMI and impaired glucose tolerance (IGT). Methods: The study included 118 postmenopausal women. 38 women (cases) were overweight/obese, BMI 28-38, aged 48-70 years, with normal (n=21) glucose metabolism (NGT) and IGT (n=17). 80 women (controls) were healthy non obese with normal OGTT and of similar age. OGTT with 75gr glucose was performed in the morning. OxLDL and anti-oxLDL were measured using ELISA. Serum glucose, total-cholesterol and LDL were measured in an automated analyzer (Roche Diagnostics). Results: Total cholesterol, LDL and oxLDL serum levels were significantly elevated in overweight/obese women as compared to controls (p<0.001). Total cholesterol, LDL and oxLDL did not differ between overweight/obese women with NGT and those with IGT. Anti-oxLDL were elevated in overweight/obese subjects (500+519) compared to controls (328+53) but not significantly. A positive correlation was found between oxLDL and anti-oxLDL (r=+0.337, p=0.002), between oxLDL and LDL (r=+0.549, p<0.001), and between oxLDL and total cholesterol (r=+0.493, p≤0.001) in controls. On the contrary no significant correlations were observed in overweight/obese individuals. Multiple linear regression analysis revealed that postmenopausal obesity was a statistical significant predictor of serum oxLDL levels (p<0.001) adjusting for age and serum levels of total cholesterol and anti-oxLDL. Conclusions: Postmenopausal obesity is involved, at least in part, in the process of LDL oxidation. Further clinical evaluation is under investigation. *Both authors have contributed equally to this work
PO31 THROMBOGENIC RISK FACTORS PO31-517
PO30-515
PROGRESSION OF CAROTID INTIMA-MEDIA THICKNESS ASSOCIATED WITH OVERWEIGHT AND OBESITY IN NON-DIABETIC SUBJECTS
OXIDIZED LDL AND POSTMENOPAUSAL OBESITY
IDENTIFICATION OF PROTEINS SECRETED BY ARTERY BIOPSIES IN THE SEARCH FOR ATHEROTHROMBOTIC BIOMARKERS
A. Jimenez-Corona 1 , R. Lopez-Ridaura 1 , M.C. Escamilla-Nunez 2 , C. Gonzalez-Villalpando 1,3 . 1 Chronic Diseases Branch, National Institute of Health, Cuernavaca, Morelos, Mexico; 2 Environmental Health Branch, National Institute of Health, Cuernavaca, Morelos, Mexico; 3 Diabetes Studies Center, Mexico, City, Mexico
G. Alvarez-Llamas 1 , F. de la Cuesta 1 , V. Darde 1 , A. Donado 2 , L.R. Padial 3 , A.G. Pinto 2 , M.G. Barderas 4 , F. Vivanco 1 . 1 Inmunology Department, Fundacion Jimenez Diaz, Madrid, Spain; 2 Cardiac Surgery Department, Hospital Gregorio Maranon, Madrid, Spain; 3 Cardiology Department, Hospital Virgen de la Salud, Toledo, Spain; 4 Vascular Physiopathology Laboratory, Hospital Nacional de Paraplejicos SESCAM, Toledo, Madrid
Background: The intima-media thickness (IMT) of both the common carotid artery (CCA) and the internal carotid artery (ICA) has been used as an index of subclinical atherosclerosis and has been associated with cardiovascular disease. This study aimed to determine whether the IMT is independently associated with overweight and obesity in non-diabetic subjects. Methods: The Mexico City Diabetes Study is a population-based study started in 1990, which includes subjects aged 35 years at baseline. For this analysis, we included non-diabetic subjects examined in 1994 and 1997. B-mode ultrasound examination of extracranial carotid arteries was performed using the same scanning, reading and image conservation protocol as that used in the Cardiovascular Health Study.
Background and aims: One of the mayor challenges of cardiovascular medicine is the capability to predict who is at risk of an acute thrombotic event, for which there is a clear need to discover prognostic markers. In this way, the study of proteins secreted by the atherome plaque could highlight biomarkers of the pathology present in patients’ blood, so as to facilitate early diagnosis and treatment. The present work is aimed at the proteomic analysis of artery tissues secretome in the search for atherothrombotic biomarkers. Methods: Artery biopsies (coronary, radial, mammary) from necropsies or bypass surgery patients were maintained in RPMI medium, following a culture protocol where detection of high abundant proteins (plasma con-
77th Congress of the European Atherosclerosis Society, April 26–29, 2008, Istanbul, Turkey
POSTER SESSIONS
A. Onat 1,2 , G. Hergenc 3 , D. Dursunoglu 4 , Z. Kucukkdurmaz 5 , S. Bulur 6 , G. Can 2 . 1 Turkish Cardiology Society,Istanbul; 2 Istanbul University Cerrahpasa Medical Faculty Cardiology Department, Istanbul; 3 Yildiz Technical University Biology Department, Istanbul; 4 Pamukkale University Medical Faculty Cardiology Department, Denizli; 5 Gaziantep University Medical Faculty Cardiology Department, Gaziantep; 6 Duzce University Medical Faculty Cardiology Department, Duzce
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RELATIVELY HIGH LEVELS OF SERUM ADIPONECTIN IN OBESE INDIVIDUALS, A MARKER OF ADIPONECTIN RESISTANCE: ROLE OF SEX HORMONE-BINDING GLOBULIN
Objectives: It is unclear whether serum adiponectin concentrations diminish linearly with increasing adiposity and which factors are codeterminants of this association. These issues were investigated cross-sectionally in a general population sample representative of middle-aged and elderly Turkish adults. Methods: In 1199 men and women, serum total adiponectin was assayed by ELISA. ‘Adiponectin adequacy’ was defined as serum concentrations of >8.3 in men, >10.6 µg/ml in women, corresponding to 45th percentile in either gender. Results: Serum adiponectin values in men, though declining significantly in transition from the bottom to the mid tertile of body mass index (BMI), waist circumference (WC) and hip circumference (HC), were similar in the two respective upper tertiles. In women, adiponectin was not significantly different in any tertile of these indices. Serum adiponectin significantly correlated with BMI, WC, or HC within the bottom tertiles and the top tertile of female HC but, otherwise, not within the upper two tertiles. In multiple logistic regression analyses for the likelihood of ‘adiponectin adequacy’ in two models, HDL-cholesterol and CRP were significantly associated in men, whereas in women SHBG [at significant ORs 1.5 for a 2-fold increment) and triglycerides were associated, independent of (abdominal) obesity markers and fasting insulin levels. Conclusions: Serum adiponectin is affected by SHBG independently from obesity and hyperinsulinemia, especially in obese women, which induces ‘adiponectin resistance’ and accounts for an inconsistent relationship between excess adiposity and adiponectin levels. Gender determines partial independence of anti-inflammatory properties of adiponectin from obesity and insulin resistance.
Results: A total of 1184 non-diabetic subjects (aged 49.6±8.1) were included. Mean IMT in both CCA (µm) and ICA (µm) was greater at baseline in overweight and obese subjects (defined by BMI). In multiple models, we found that both baseline and follow-up CCA-IMT were associated with overweight (β=20.9, p=0.01, and β=36.3, p<0.001, respectively) and obesity (β=24.4, p=0.008, and β=34.5, p=0.002, respectively). Regarding waist circumference, only CCA-IMT at baseline was associated with the third (β=25.2, p=0.005) and fourth quartiles (β=20.8, p=0.026). Conclusions: Our study showed a higher IMT of both CCA and ICA in overweight and obese subjects. BMI and waist circumference were independently associated with the IMT of CCA at baseline, whereas BMI predicted CCA-IMT during follow-up. These results suggest that central fat accumulation may accelerate the development of earlier clinically stages of atherosclerosis. PO30-516
E. Kassi, M. Dalamaga*, E. Faviou*, G. Hroussalas, K. Kazanis, A. Dionyssiou-Asteriou. Department of Biological Chemistry and Department of Clinical Biochemistry, Medical School, University of Athens, Athens, Greece Aim: To determine serum levels of oxidized LDL (oxLDL) and antibodies against them (anti-oxLDL) in postmenopausal women and evaluate their association with BMI and impaired glucose tolerance (IGT). Methods: The study included 118 postmenopausal women. 38 women (cases) were overweight/obese, BMI 28-38, aged 48-70 years, with normal (n=21) glucose metabolism (NGT) and IGT (n=17). 80 women (controls) were healthy non obese with normal OGTT and of similar age. OGTT with 75gr glucose was performed in the morning. OxLDL and anti-oxLDL were measured using ELISA. Serum glucose, total-cholesterol and LDL were measured in an automated analyzer (Roche Diagnostics). Results: Total cholesterol, LDL and oxLDL serum levels were significantly elevated in overweight/obese women as compared to controls (p<0.001). Total cholesterol, LDL and oxLDL did not differ between overweight/obese women with NGT and those with IGT. Anti-oxLDL were elevated in overweight/obese subjects (500+519) compared to controls (328+53) but not significantly. A positive correlation was found between oxLDL and anti-oxLDL (r=+0.337, p=0.002), between oxLDL and LDL (r=+0.549, p<0.001), and between oxLDL and total cholesterol (r=+0.493, p≤0.001) in controls. On the contrary no significant correlations were observed in overweight/obese individuals. Multiple linear regression analysis revealed that postmenopausal obesity was a statistical significant predictor of serum oxLDL levels (p<0.001) adjusting for age and serum levels of total cholesterol and anti-oxLDL. Conclusions: Postmenopausal obesity is involved, at least in part, in the process of LDL oxidation. Further clinical evaluation is under investigation. *Both authors have contributed equally to this work
PO31 THROMBOGENIC RISK FACTORS PO31-517
PO30-515
PROGRESSION OF CAROTID INTIMA-MEDIA THICKNESS ASSOCIATED WITH OVERWEIGHT AND OBESITY IN NON-DIABETIC SUBJECTS
OXIDIZED LDL AND POSTMENOPAUSAL OBESITY
IDENTIFICATION OF PROTEINS SECRETED BY ARTERY BIOPSIES IN THE SEARCH FOR ATHEROTHROMBOTIC BIOMARKERS
A. Jimenez-Corona 1 , R. Lopez-Ridaura 1 , M.C. Escamilla-Nunez 2 , C. Gonzalez-Villalpando 1,3 . 1 Chronic Diseases Branch, National Institute of Health, Cuernavaca, Morelos, Mexico; 2 Environmental Health Branch, National Institute of Health, Cuernavaca, Morelos, Mexico; 3 Diabetes Studies Center, Mexico, City, Mexico
G. Alvarez-Llamas 1 , F. de la Cuesta 1 , V. Darde 1 , A. Donado 2 , L.R. Padial 3 , A.G. Pinto 2 , M.G. Barderas 4 , F. Vivanco 1 . 1 Inmunology Department, Fundacion Jimenez Diaz, Madrid, Spain; 2 Cardiac Surgery Department, Hospital Gregorio Maranon, Madrid, Spain; 3 Cardiology Department, Hospital Virgen de la Salud, Toledo, Spain; 4 Vascular Physiopathology Laboratory, Hospital Nacional de Paraplejicos SESCAM, Toledo, Madrid
Background: The intima-media thickness (IMT) of both the common carotid artery (CCA) and the internal carotid artery (ICA) has been used as an index of subclinical atherosclerosis and has been associated with cardiovascular disease. This study aimed to determine whether the IMT is independently associated with overweight and obesity in non-diabetic subjects. Methods: The Mexico City Diabetes Study is a population-based study started in 1990, which includes subjects aged 35 years at baseline. For this analysis, we included non-diabetic subjects examined in 1994 and 1997. B-mode ultrasound examination of extracranial carotid arteries was performed using the same scanning, reading and image conservation protocol as that used in the Cardiovascular Health Study.
Background and aims: One of the mayor challenges of cardiovascular medicine is the capability to predict who is at risk of an acute thrombotic event, for which there is a clear need to discover prognostic markers. In this way, the study of proteins secreted by the atherome plaque could highlight biomarkers of the pathology present in patients’ blood, so as to facilitate early diagnosis and treatment. The present work is aimed at the proteomic analysis of artery tissues secretome in the search for atherothrombotic biomarkers. Methods: Artery biopsies (coronary, radial, mammary) from necropsies or bypass surgery patients were maintained in RPMI medium, following a culture protocol where detection of high abundant proteins (plasma con-
77th Congress of the European Atherosclerosis Society, April 26–29, 2008, Istanbul, Turkey
POSTER SESSIONS
A. Onat 1,2 , G. Hergenc 3 , D. Dursunoglu 4 , Z. Kucukkdurmaz 5 , S. Bulur 6 , G. Can 2 . 1 Turkish Cardiology Society,Istanbul; 2 Istanbul University Cerrahpasa Medical Faculty Cardiology Department, Istanbul; 3 Yildiz Technical University Biology Department, Istanbul; 4 Pamukkale University Medical Faculty Cardiology Department, Denizli; 5 Gaziantep University Medical Faculty Cardiology Department, Gaziantep; 6 Duzce University Medical Faculty Cardiology Department, Duzce
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