- Email: [email protected]
P-051: Leukocyte telomere length is associated with lean mass: data from the Berlin Aging Study II (BASE-II)
Poster presentations / European Geriatric Medicine 6S1 (2015) S32–S156
Design: A longitudinal twin study with baseline and follow-up measurements of LTL and insulin resistance (HOMA-IR) over an average follow-up of 12 years. Setting: A subset of the population-based national Danish Twin Registry Participants: 338 twin pairs of the same sex (184 MZ and 154 DZ) aged 37.4±9.6 years at baseline examination. Main outcome measure: Relationship between LTL and HOMA-IR and changes in both measurements during the follow up period. Results: Baseline HOMA-IR was not associated with changes in LTL (attrition) over the follow up period, whereas baseline LTL was associated with changes in HOMA-IR during this period. The shorter the LTL at baseline the more pronounced was the increase in HOMA-IR over the follow-up period (p < 0.001); this effect was additive to that of BMI. The co-twin with the shorter baseline LTL displayed a higher HOMA-IR at follow-up than the co-twin with the longer LTL. A larger difference in LTL between the co-twins at baseline was associated with a greater probability that the cotwin with the shorter LTL would develop a higher HOMA-IR at follow-up. Conclusion: These findings suggest that individuals with short LTL are more likely to develop insulin resistance later in life. P-050 Independent predictors of hospital admission in emergency department patients younger and older than 70 years of age J. Lucke1 , J. de Gelder2 , B. de Groot1 , F. Clarijs2 , C. Heringhaus1 , A.J. Fogteloo1 , G. Blauw3 , S. Mooijaart3 1 Leiden University Medical Centre, Leiden, Netherlands; 2 LUMC, Leiden, Netherlands; 3 Netherlands Introduction: Independent predictors of hospital admission have been investigated in patients on the Emergency Department (ED), but it is unknown whether these predictors are different for patients above and below 70 years old. In this study we compared patient characteristics between patients younger and older than 70 years and investigated whether independent predictors of hospital admission are different in these patient groups. Method: A retrospective cohort study of all ED visits in a tertiary hospital in 2012. Patient characteristics including way of arrival, presenting complaint and urgency of the complaint were analysed and stratified into age groups. Multivariable logistic regression was used to identify independent predictors and discriminative performance was quantified by area under the curve (AUC) analysis in both groups. Results: 4255 patients >70 years and 17319 patients <70 years were included. 45% of the older patients were hospitalized and 25% of the younger patients. Between age groups the same independent predictors were found such as triage category and amount of registered vital parameters, mostly reflecting illness severity. The prediction model for hospitalisation had a higher discriminative performance in young patients with an AUC of 0.85 (0.84–0.85), the model in old patients had an AUC of 0.76 (0.75–0.78), with both models having good predicting capabilities. Conclusion: Independent predictors of hospital admission are similar in patients younger and older than 70 years in the ED, but perform better in younger patients. This suggests that in older patients other factors such as cognition and functional status, may play a role.
S45
P-051 Leukocyte telomere length is associated with lean mass: data from the Berlin Aging Study II (BASE-II) A. Meyer1 , B. Salewsky2 , E. Steinhagen-Thiessen3 , K. Norman3 , I. Demuth2 1 Charit´e-Universit¨ atsmedizin Berlin, Berlin, Germany; 2 Institute of Medical and Human Genetics, Charit´e-Universit¨ atsmedizin Berlin, Berlin, Germany; 3 Research Group on Geriatrics, Charit´eUniversit¨ atsmedizin Berlin, Berlin, Germany Background: Age-related loss of muscle mass is an increasing problem in in our aging society, affecting physical ability. Telomere length has been recognized as a marker of biological age on the population level. Objective: Here we evaluated the rarely examined relationship between lean mass and relative leukocyte telomere length (rLTL) in 1,398 participants of the Berlin Aging Study II (mean age 68.2±3.7 years, 49.6% men). Methods: The determination of rLTL was carried out by real time PCR. Lean mass was estimated by dual X-ray absorptiometry and examined as leg lean mass (LLM), appendicular lean mass (ALM), and ALM corrected for body mass index (ALMBMI). Results: Highly significant correlations (p < 0.001) of rLTL and ALM (r = 0.248), ALMBMI (r = 0.254), and LLM (r = 0.263) were found. Associations remained significant in linear models adjusted for age, gender, BMI, low-grade inflammation, life style factors and morbidities: ALM (b = 0.844, p = 0.009), ALMBMI (b = 0.032, p = 0.011), and LLM (b = 0.967, p < 0.001). Shorter rLTL, advanced age, female sex, sedentary lifestyle and elevated CRP level were associated with lower lean mass. Conclusion: Short telomeres were associated with low lean mass. Our results indicate that rLTL may be a risk factor for loss of lean mass. To confirm the association between telomere attrition and loss of LLM and ALMBMI, which are highly relevant for physical ability, further research should examine this subject in a longitudinal context. P-052 Loss of fertility in aging males due to increased testicular estradiol production A. Rosenstrauch1 , A. Degen2 1 Achva Academic College, Shikmim, Israel; 2 Ben-Gurion University, Beer Sheva, Israel In aging man, other mammals and birds, loss of fertility is accompanied by high levels of plasma estradiol and concomitant reduced levels of both plasma LH and testosterone. Hormonal levels are regulated by the negative feedback mechanism as follows: (1) plasma testosterone is aromatized to estradiol in the brain, (2) which attaches to hypothalamic estradiol-receptors; (3) the estradiol-receptor complex results in the reduction of GnRH secretion (4) that leads to decrease in plasma LH and FSH levels and the consequent reduction of testicular testosterone level. We examined fertility changes in the aging rooster, an animal that reaches peak fertility at a relatively young age. In addition, we examined fertility changes in aging roosters treated with clomiphene citrate, an anti-estrogen. We collected pituitary glands of high fertility (32 weeks), and aged roosters (70 weeks) with and without clomiphene citrate treatment to determine number of gonadotrophs in the pituitary. In addition, plasma, LH, testosterone and 17b-estradiol were measured using radioimmunoassays. In 70 week old males, the number of gonadotrophs per mm2 , plasma LH concentrations and plasma testosterone concentrations were reduced by 56%, 39% and 40%, respectively, when compared to 32 week old roosters. However, the clomiphene citrate treated males had similar measurements as the 32 week old roosters.
Design: A longitudinal twin study with baseline and follow-up measurements of LTL and insulin resistance (HOMA-IR) over an average follow-up of 12 years. Setting: A subset of the population-based national Danish Twin Registry Participants: 338 twin pairs of the same sex (184 MZ and 154 DZ) aged 37.4±9.6 years at baseline examination. Main outcome measure: Relationship between LTL and HOMA-IR and changes in both measurements during the follow up period. Results: Baseline HOMA-IR was not associated with changes in LTL (attrition) over the follow up period, whereas baseline LTL was associated with changes in HOMA-IR during this period. The shorter the LTL at baseline the more pronounced was the increase in HOMA-IR over the follow-up period (p < 0.001); this effect was additive to that of BMI. The co-twin with the shorter baseline LTL displayed a higher HOMA-IR at follow-up than the co-twin with the longer LTL. A larger difference in LTL between the co-twins at baseline was associated with a greater probability that the cotwin with the shorter LTL would develop a higher HOMA-IR at follow-up. Conclusion: These findings suggest that individuals with short LTL are more likely to develop insulin resistance later in life. P-050 Independent predictors of hospital admission in emergency department patients younger and older than 70 years of age J. Lucke1 , J. de Gelder2 , B. de Groot1 , F. Clarijs2 , C. Heringhaus1 , A.J. Fogteloo1 , G. Blauw3 , S. Mooijaart3 1 Leiden University Medical Centre, Leiden, Netherlands; 2 LUMC, Leiden, Netherlands; 3 Netherlands Introduction: Independent predictors of hospital admission have been investigated in patients on the Emergency Department (ED), but it is unknown whether these predictors are different for patients above and below 70 years old. In this study we compared patient characteristics between patients younger and older than 70 years and investigated whether independent predictors of hospital admission are different in these patient groups. Method: A retrospective cohort study of all ED visits in a tertiary hospital in 2012. Patient characteristics including way of arrival, presenting complaint and urgency of the complaint were analysed and stratified into age groups. Multivariable logistic regression was used to identify independent predictors and discriminative performance was quantified by area under the curve (AUC) analysis in both groups. Results: 4255 patients >70 years and 17319 patients <70 years were included. 45% of the older patients were hospitalized and 25% of the younger patients. Between age groups the same independent predictors were found such as triage category and amount of registered vital parameters, mostly reflecting illness severity. The prediction model for hospitalisation had a higher discriminative performance in young patients with an AUC of 0.85 (0.84–0.85), the model in old patients had an AUC of 0.76 (0.75–0.78), with both models having good predicting capabilities. Conclusion: Independent predictors of hospital admission are similar in patients younger and older than 70 years in the ED, but perform better in younger patients. This suggests that in older patients other factors such as cognition and functional status, may play a role.
S45
P-051 Leukocyte telomere length is associated with lean mass: data from the Berlin Aging Study II (BASE-II) A. Meyer1 , B. Salewsky2 , E. Steinhagen-Thiessen3 , K. Norman3 , I. Demuth2 1 Charit´e-Universit¨ atsmedizin Berlin, Berlin, Germany; 2 Institute of Medical and Human Genetics, Charit´e-Universit¨ atsmedizin Berlin, Berlin, Germany; 3 Research Group on Geriatrics, Charit´eUniversit¨ atsmedizin Berlin, Berlin, Germany Background: Age-related loss of muscle mass is an increasing problem in in our aging society, affecting physical ability. Telomere length has been recognized as a marker of biological age on the population level. Objective: Here we evaluated the rarely examined relationship between lean mass and relative leukocyte telomere length (rLTL) in 1,398 participants of the Berlin Aging Study II (mean age 68.2±3.7 years, 49.6% men). Methods: The determination of rLTL was carried out by real time PCR. Lean mass was estimated by dual X-ray absorptiometry and examined as leg lean mass (LLM), appendicular lean mass (ALM), and ALM corrected for body mass index (ALMBMI). Results: Highly significant correlations (p < 0.001) of rLTL and ALM (r = 0.248), ALMBMI (r = 0.254), and LLM (r = 0.263) were found. Associations remained significant in linear models adjusted for age, gender, BMI, low-grade inflammation, life style factors and morbidities: ALM (b = 0.844, p = 0.009), ALMBMI (b = 0.032, p = 0.011), and LLM (b = 0.967, p < 0.001). Shorter rLTL, advanced age, female sex, sedentary lifestyle and elevated CRP level were associated with lower lean mass. Conclusion: Short telomeres were associated with low lean mass. Our results indicate that rLTL may be a risk factor for loss of lean mass. To confirm the association between telomere attrition and loss of LLM and ALMBMI, which are highly relevant for physical ability, further research should examine this subject in a longitudinal context. P-052 Loss of fertility in aging males due to increased testicular estradiol production A. Rosenstrauch1 , A. Degen2 1 Achva Academic College, Shikmim, Israel; 2 Ben-Gurion University, Beer Sheva, Israel In aging man, other mammals and birds, loss of fertility is accompanied by high levels of plasma estradiol and concomitant reduced levels of both plasma LH and testosterone. Hormonal levels are regulated by the negative feedback mechanism as follows: (1) plasma testosterone is aromatized to estradiol in the brain, (2) which attaches to hypothalamic estradiol-receptors; (3) the estradiol-receptor complex results in the reduction of GnRH secretion (4) that leads to decrease in plasma LH and FSH levels and the consequent reduction of testicular testosterone level. We examined fertility changes in the aging rooster, an animal that reaches peak fertility at a relatively young age. In addition, we examined fertility changes in aging roosters treated with clomiphene citrate, an anti-estrogen. We collected pituitary glands of high fertility (32 weeks), and aged roosters (70 weeks) with and without clomiphene citrate treatment to determine number of gonadotrophs in the pituitary. In addition, plasma, LH, testosterone and 17b-estradiol were measured using radioimmunoassays. In 70 week old males, the number of gonadotrophs per mm2 , plasma LH concentrations and plasma testosterone concentrations were reduced by 56%, 39% and 40%, respectively, when compared to 32 week old roosters. However, the clomiphene citrate treated males had similar measurements as the 32 week old roosters.