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P-261
and implantation rates were compared in both the groups in IVF and ICSI cycles. Statistical significance was determined using Students t test. RESULTS: The mean age of the female patient was 33.8 years (⫾5.05) with no statistical difference amongst the groups. The incidence of suboptimal fertilization rate (less than or equal to 50%, Group A) was 14.0% for IVF cycles and 19.0% for ICSI cycles. The results are shown in Table 1.
CONCLUSION: Irrespective of the assisted reproductive technique applied (IVF or ICSI), cycles that exhibit sub-optimal fertilization rate are likely to provide embryos with much lower implantation potential and viability. This data strongly suggests that fertilization rate is a reliable parameter to predict the outcome of IVF and ICSI cycles. Supported by: None.
P-261 EARLY EMBRYO CLEAVAGE: A VALUABLE INDICATOR OF EMBRYO VIABILITY. G. N. Allahbadia, G. N. Gandhi, K. S. Kadam, S. Parab, A. Nagwekar, S. Virk. Rotunda - The Center For Human Reproduction, Mumbai, India. CONCLUSION: To our knowledge, this is the first report of yeast colonization in human embryos with Candida dubliniensis. This species is identified in approximately 2% of cases involving yeast infections and is also often misdiagnosed as Candida albicans. Follicular fluid has previously been used to supplement culture media used for IVM due to its hormonal and maturational factors. Immature oocytes are capable of spontaneous maturation in vitro, however, the rate of maturity is less than 40%. It is unknown, what vector caused the presence of yeast spores in follicular fluid. However, it seems that an actively developing cells, as in an embryo undergoing cleavage, are necessary for the yeast to transform to its active hyphal form. Attempts to remove the yeast were necessary due to the unknown consequences of transferring those embryos into the uterus. Supported by: Infertility& IVF Center.
P-260 FERTILIZATION RATE - A RELIABLE PREDICTOR OF SUCCESSFUL OUTCOME IN IVF/ICSI CYCLES. G. N. Allahbadia, G. N. Gandhi, K. S. Kadam, A. Nagwekar, S. Virk, K. Kaur. Rotunda - The Center For Human Reproduction, Mumbai, India. OBJECTIVE: The aim of this study was to analyze the influence of fertilization rate in IVF as well as ICSI cycles. DESIGN: This is a retrospective study conducted by an academically oriented private ART center. MATERIALS AND METHODS: This work included 250 IVF cycles and 58 ICSI cycles. Patients were grouped based on fertilization rate. Patients with fertilization rate less than or equal to 50% were named Group A and patients with more than 50% fertilization rate were named Group B. Patients more than 35 years of age and with less than 4 oocytes retrieved were excluded from the study. The stimulation protocol, age, number of oocytes retrieved, fertilization rate, cleavage rate, grade of embryos, pregnancy rate
FERTILITY & STERILITY威
OBJECTIVE: High pregnancy rates have been noted after Oocyte Donation (OD). The incidence of multiple pregnancies after OD is also very high. Judicious reduction in the number of embryos transferred is the only method to decrease multiple gestations. The need for selecting the embryos with a high implantation potential is paramount when a large number of them with similar morphological characteristics are available for transfer. The present study investigated whether early embryo cleavage could be used as an additional marker of embryo viability. DESIGN: This is a retrospective study conducted in a private, academically oriented ART center. MATERIALS AND METHODS: We analyzed results for our IVF Oocyte Donation cycles from January 2005 to March 2006. Cleavage to the 2-cell stage was assessed at 25-28 hours post insemination. Cycles with any embryos that had cleaved at this time point were designated as “early cleavage” (EC) while cycles in which there were no embryos that had reached the 2-cell stage were designated as “non-early cleavage” (NEC). Early cleaving embryos were selected and preferentially transferred. RESULTS: Early cleavage was observed in a total of 117 (40.6%) of the 288 cycles assessed. In the EC group, at least two of the transferred embryos were early cleaving. In the NEC group, there were no early cleaving embryos available for transfer. A maximum of four embryos were transferred in all the cycles. No differences between the groups were found when comparing age, stimulation protocol and semen characteristics. A significantly higher pregnancy rate was observed in the EC group than the NEC group (69.2% versus 26.5%). The implantation rate was also higher in the EC group (23% versus 15%). CONCLUSION: The current study provides clear evidence that early cleaving embryos have a significantly higher developmental competence. Thus, early embryo cleavage can, together with conventional morphological criteria, serve as a simple, non-invasive method for selection of embryos with high implantation potential. Supported by: None
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CONCLUSION: Irrespective of the assisted reproductive technique applied (IVF or ICSI), cycles that exhibit sub-optimal fertilization rate are likely to provide embryos with much lower implantation potential and viability. This data strongly suggests that fertilization rate is a reliable parameter to predict the outcome of IVF and ICSI cycles. Supported by: None.
P-261 EARLY EMBRYO CLEAVAGE: A VALUABLE INDICATOR OF EMBRYO VIABILITY. G. N. Allahbadia, G. N. Gandhi, K. S. Kadam, S. Parab, A. Nagwekar, S. Virk. Rotunda - The Center For Human Reproduction, Mumbai, India. CONCLUSION: To our knowledge, this is the first report of yeast colonization in human embryos with Candida dubliniensis. This species is identified in approximately 2% of cases involving yeast infections and is also often misdiagnosed as Candida albicans. Follicular fluid has previously been used to supplement culture media used for IVM due to its hormonal and maturational factors. Immature oocytes are capable of spontaneous maturation in vitro, however, the rate of maturity is less than 40%. It is unknown, what vector caused the presence of yeast spores in follicular fluid. However, it seems that an actively developing cells, as in an embryo undergoing cleavage, are necessary for the yeast to transform to its active hyphal form. Attempts to remove the yeast were necessary due to the unknown consequences of transferring those embryos into the uterus. Supported by: Infertility& IVF Center.
P-260 FERTILIZATION RATE - A RELIABLE PREDICTOR OF SUCCESSFUL OUTCOME IN IVF/ICSI CYCLES. G. N. Allahbadia, G. N. Gandhi, K. S. Kadam, A. Nagwekar, S. Virk, K. Kaur. Rotunda - The Center For Human Reproduction, Mumbai, India. OBJECTIVE: The aim of this study was to analyze the influence of fertilization rate in IVF as well as ICSI cycles. DESIGN: This is a retrospective study conducted by an academically oriented private ART center. MATERIALS AND METHODS: This work included 250 IVF cycles and 58 ICSI cycles. Patients were grouped based on fertilization rate. Patients with fertilization rate less than or equal to 50% were named Group A and patients with more than 50% fertilization rate were named Group B. Patients more than 35 years of age and with less than 4 oocytes retrieved were excluded from the study. The stimulation protocol, age, number of oocytes retrieved, fertilization rate, cleavage rate, grade of embryos, pregnancy rate
FERTILITY & STERILITY威
OBJECTIVE: High pregnancy rates have been noted after Oocyte Donation (OD). The incidence of multiple pregnancies after OD is also very high. Judicious reduction in the number of embryos transferred is the only method to decrease multiple gestations. The need for selecting the embryos with a high implantation potential is paramount when a large number of them with similar morphological characteristics are available for transfer. The present study investigated whether early embryo cleavage could be used as an additional marker of embryo viability. DESIGN: This is a retrospective study conducted in a private, academically oriented ART center. MATERIALS AND METHODS: We analyzed results for our IVF Oocyte Donation cycles from January 2005 to March 2006. Cleavage to the 2-cell stage was assessed at 25-28 hours post insemination. Cycles with any embryos that had cleaved at this time point were designated as “early cleavage” (EC) while cycles in which there were no embryos that had reached the 2-cell stage were designated as “non-early cleavage” (NEC). Early cleaving embryos were selected and preferentially transferred. RESULTS: Early cleavage was observed in a total of 117 (40.6%) of the 288 cycles assessed. In the EC group, at least two of the transferred embryos were early cleaving. In the NEC group, there were no early cleaving embryos available for transfer. A maximum of four embryos were transferred in all the cycles. No differences between the groups were found when comparing age, stimulation protocol and semen characteristics. A significantly higher pregnancy rate was observed in the EC group than the NEC group (69.2% versus 26.5%). The implantation rate was also higher in the EC group (23% versus 15%). CONCLUSION: The current study provides clear evidence that early cleaving embryos have a significantly higher developmental competence. Thus, early embryo cleavage can, together with conventional morphological criteria, serve as a simple, non-invasive method for selection of embryos with high implantation potential. Supported by: None
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