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P 27 Comparison of cardiac function parameters measured longitudinally in pregnancies with late-onset preeclampsia and normotensive pregnancies
Abstracts / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 9 (2017) 36–63
P 25 Vesicle-based resveratrol formulation for the topical treatment of Trichomonas vaginalis caused vaginal infection and inflammation Purusotam Basnet a, May Wenche Jøraholmen b, Ganesh Acharya a,c, Natasa Skalko-Basnet b (a University of Tromsø, Department of Clinical Medicine, Tromsø, Norway, b University of Tromsø, Department of Pharmacy, Tromsø, Norway, c Karolinska Institutet, Department of Clinical Science Intervention and Technology, Stockholm, Sweden) Introduction: Untreated vaginal inflammation can cause miscarriage, preterm labour, placental dysfunction and perinatal complications. Trichomonas vaginalis infection and inflammation are of serious concerns due to lipophosphoglycan (LPG) which triggers selective up regulation of cytokines, especially IL-8, in the female reproductive tract [1]. There are several anti-inflammatory and anti-microbial drugs currently available on the market, however, there is no general consensus regarding safe formulation especially for use in early pregnancy and considering microbial resistance. Resveratrol, a natural polyphenol, showed promising results comparing to clinically used drug metronidazole [2]. However, due to poor solubility and low bioavailability of resveratrol, its clinical application remains challenging. Our aim was to develop a non-teratogenic and nontoxic formulation which can be used in early pregnancy for the treatment of Trichomonas infection and inflammation. Methods: Liposomes carrying resveratrol were characterized for their stability, size and entrapment efficiency. Antioxidant activities were evaluated by DPPH/ABTS+ and SOD activities. Antioxidant and anti-inflammatory activities of resveratrol and their corresponding vesicle-based formulation were compared. Anti-inflammatory activities were evaluated by measuring LPS-induced NO and proinflammatory cytokines such as IL-1b, TNF-a, IL-8 in macrophages (J774.1) and LPG-induced cytokines IL-8 in human vaginal cells lines (VK2/E6E7). Results: Phospholipid-based vesicles, liposomes, of average size around 100 nm were found to be stable with a high resveratrol load (77%). Liposomal resveratrol was found to be 3 folds more potent than resveratrol in inhibiting NO production and up to 30% proinflammatory cytokines in macrophages were inhibited. IL-8 was inhibited up to 36% by the liposomal resveratrol in human vaginal cell lines VK2/E6E7 as compared to resveratrol. Conclusion: Standardized liposomal resveratrol might be an appropriate, effective and safe topical formulation for the treatment of vaginal infection and inflammation against trichomoniasis in early pregnancy. References [1] Fichorova RN et al. Infection Immunity ];74(10):5773–9. [2] Mallo N et al. Antimicrob. Agents Chemother. ];57(6):2476–84.
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doi:10.1016/j.preghy.2017.07.104
P 27 Comparison of cardiac function parameters measured longitudinally in pregnancies with late-onset preeclampsia and normotensive pregnancies Frederikke Lihme a, Lisa Persson a, Saima Basit a, Jacob Alexander Lykke b, Kasper Pihl c, Karen Halse c, Jan Wohlfahrt a, Heather Boyd a (a Statens Serum Institut, Department of Epidemiology Research, Copenhagen, Denmark, b Rigshospitalet, Department of Gynaecology and Obstetrics, Copenhagen, Denmark, c Hvidovre Hospital, Department of Gynaecology and obstetrics, Copenhagen, Denmark) Introduction: The preeclampsia (PE) syndrome almost certainly encompasses multiple disease entities. Cardiovascular dysfunction is well documented in early-onset (GA < 34 weeks + 0 days) PE. Whether similar dysfunction characterizes PE with later onset, which constitutes approximately 75% of PE, is less clear. Objectives: To compare repeated cardiac function measurements (stroke volume, cardiac output, and total vascular resistance) measured in normotensive pregnancies and pregnancies complicated with late-onset PE. Patients and methods: The PEACH (PreEclampsia, Angiogenesis, Cardiac dysfunction and Hypertension) study is an ongoing longitudinal prospective study being conducted at two university hospitals in Copenhagen, Denmark. PEACH recruits women presenting to the obstetrics departments of these hospitals with signs of hypertensive disorders of pregnancy (HDP) and follows them until the end of pregnancy with serial blood tests and repeated cardiac function measurements obtained using the USCOM-1A device. The study also follows normotensive pregnant women frequency matched to the women in the HDP group and assesses their cardiac function in gestational weeks 28, 35, 38 and 40. USCOM data on cardiac function are analyzed using linear mixed models that include a random personal effect (woman-specific effect), a time-dependent gestational age effect (a trend describing how normal cardiac function changes over the course of pregnancy) and a time-dependent PE effect (how PE – overall or by subtypes – affects cardiac function at a given point during pregnancy). Results and conclusion: We compare cardiac function measurements and corresponding indexed values from the first 100 women recruited to the PEACH study with late-onset PE and 100 appropriate controls, at given points during pregnancy, adjusting for age, parity, hospital, ethnicity and other potential confounders. The resulting profiles should help to clarify what role cardiac function monitoring can play in prediction, triage, diagnosis and/or management of lateonset PE. doi:10.1016/j.preghy.2017.07.105
doi:10.1016/j.preghy.2017.07.103
P 26 Cesarean delivery in preeclamptic patients – clinical assessment focusing on coagulation. Nikita Iakovlev a,b, Timur Kurmanbaev c,b, Roza Nabiullina d, Ilshat Mustafin d, Lev Kozlov b, Albir Khasanov a,b (a Republican Clinical Hospital, Labor and delivery, Kazan, Russian Federation, b Kazan State Medicla University, Obstetrics and gynecology, Kazan, Russian Federation , c City Hospital N 16, Labor and delivery, Kazan, Russian Federation , d Kazan State Medicla University, Biochemistry, Kazan, Russian Federation) This presentation has now been withdrawn.
P 28 Plasma Guanylate-Binding Protein-1 concentrations during pregnancy and preeclampsia Joost H.N. Schuitemaker a,b, Thomas I.F.H. Cremers c, Mariëlle G. van Pampus d, Sicco Scherjon e, Marijke Faas b,e (a IQ Products BV, Research & Development, Groningen, The Netherlands, b University Medical Center Groningen, Division of Medical Biology, Dept. of Pathology and Medical Biology, Groningen, The Netherlands, c University of Groningen, Dept. of Pharmaceutical Analysis, Groningen, The Netherlands, d Onze Lieve Vrouwe Gasthuis, Dept. of Obstetrics and Gynecology, Amsterdam, The Netherlands, e University Medical Center Groningen, Dept. of Obstetrics and Gynecology, Groningen, The Netherlands)
P 25 Vesicle-based resveratrol formulation for the topical treatment of Trichomonas vaginalis caused vaginal infection and inflammation Purusotam Basnet a, May Wenche Jøraholmen b, Ganesh Acharya a,c, Natasa Skalko-Basnet b (a University of Tromsø, Department of Clinical Medicine, Tromsø, Norway, b University of Tromsø, Department of Pharmacy, Tromsø, Norway, c Karolinska Institutet, Department of Clinical Science Intervention and Technology, Stockholm, Sweden) Introduction: Untreated vaginal inflammation can cause miscarriage, preterm labour, placental dysfunction and perinatal complications. Trichomonas vaginalis infection and inflammation are of serious concerns due to lipophosphoglycan (LPG) which triggers selective up regulation of cytokines, especially IL-8, in the female reproductive tract [1]. There are several anti-inflammatory and anti-microbial drugs currently available on the market, however, there is no general consensus regarding safe formulation especially for use in early pregnancy and considering microbial resistance. Resveratrol, a natural polyphenol, showed promising results comparing to clinically used drug metronidazole [2]. However, due to poor solubility and low bioavailability of resveratrol, its clinical application remains challenging. Our aim was to develop a non-teratogenic and nontoxic formulation which can be used in early pregnancy for the treatment of Trichomonas infection and inflammation. Methods: Liposomes carrying resveratrol were characterized for their stability, size and entrapment efficiency. Antioxidant activities were evaluated by DPPH/ABTS+ and SOD activities. Antioxidant and anti-inflammatory activities of resveratrol and their corresponding vesicle-based formulation were compared. Anti-inflammatory activities were evaluated by measuring LPS-induced NO and proinflammatory cytokines such as IL-1b, TNF-a, IL-8 in macrophages (J774.1) and LPG-induced cytokines IL-8 in human vaginal cells lines (VK2/E6E7). Results: Phospholipid-based vesicles, liposomes, of average size around 100 nm were found to be stable with a high resveratrol load (77%). Liposomal resveratrol was found to be 3 folds more potent than resveratrol in inhibiting NO production and up to 30% proinflammatory cytokines in macrophages were inhibited. IL-8 was inhibited up to 36% by the liposomal resveratrol in human vaginal cell lines VK2/E6E7 as compared to resveratrol. Conclusion: Standardized liposomal resveratrol might be an appropriate, effective and safe topical formulation for the treatment of vaginal infection and inflammation against trichomoniasis in early pregnancy. References [1] Fichorova RN et al. Infection Immunity ];74(10):5773–9. [2] Mallo N et al. Antimicrob. Agents Chemother. ];57(6):2476–84.
49
doi:10.1016/j.preghy.2017.07.104
P 27 Comparison of cardiac function parameters measured longitudinally in pregnancies with late-onset preeclampsia and normotensive pregnancies Frederikke Lihme a, Lisa Persson a, Saima Basit a, Jacob Alexander Lykke b, Kasper Pihl c, Karen Halse c, Jan Wohlfahrt a, Heather Boyd a (a Statens Serum Institut, Department of Epidemiology Research, Copenhagen, Denmark, b Rigshospitalet, Department of Gynaecology and Obstetrics, Copenhagen, Denmark, c Hvidovre Hospital, Department of Gynaecology and obstetrics, Copenhagen, Denmark) Introduction: The preeclampsia (PE) syndrome almost certainly encompasses multiple disease entities. Cardiovascular dysfunction is well documented in early-onset (GA < 34 weeks + 0 days) PE. Whether similar dysfunction characterizes PE with later onset, which constitutes approximately 75% of PE, is less clear. Objectives: To compare repeated cardiac function measurements (stroke volume, cardiac output, and total vascular resistance) measured in normotensive pregnancies and pregnancies complicated with late-onset PE. Patients and methods: The PEACH (PreEclampsia, Angiogenesis, Cardiac dysfunction and Hypertension) study is an ongoing longitudinal prospective study being conducted at two university hospitals in Copenhagen, Denmark. PEACH recruits women presenting to the obstetrics departments of these hospitals with signs of hypertensive disorders of pregnancy (HDP) and follows them until the end of pregnancy with serial blood tests and repeated cardiac function measurements obtained using the USCOM-1A device. The study also follows normotensive pregnant women frequency matched to the women in the HDP group and assesses their cardiac function in gestational weeks 28, 35, 38 and 40. USCOM data on cardiac function are analyzed using linear mixed models that include a random personal effect (woman-specific effect), a time-dependent gestational age effect (a trend describing how normal cardiac function changes over the course of pregnancy) and a time-dependent PE effect (how PE – overall or by subtypes – affects cardiac function at a given point during pregnancy). Results and conclusion: We compare cardiac function measurements and corresponding indexed values from the first 100 women recruited to the PEACH study with late-onset PE and 100 appropriate controls, at given points during pregnancy, adjusting for age, parity, hospital, ethnicity and other potential confounders. The resulting profiles should help to clarify what role cardiac function monitoring can play in prediction, triage, diagnosis and/or management of lateonset PE. doi:10.1016/j.preghy.2017.07.105
doi:10.1016/j.preghy.2017.07.103
P 26 Cesarean delivery in preeclamptic patients – clinical assessment focusing on coagulation. Nikita Iakovlev a,b, Timur Kurmanbaev c,b, Roza Nabiullina d, Ilshat Mustafin d, Lev Kozlov b, Albir Khasanov a,b (a Republican Clinical Hospital, Labor and delivery, Kazan, Russian Federation, b Kazan State Medicla University, Obstetrics and gynecology, Kazan, Russian Federation , c City Hospital N 16, Labor and delivery, Kazan, Russian Federation , d Kazan State Medicla University, Biochemistry, Kazan, Russian Federation) This presentation has now been withdrawn.
P 28 Plasma Guanylate-Binding Protein-1 concentrations during pregnancy and preeclampsia Joost H.N. Schuitemaker a,b, Thomas I.F.H. Cremers c, Mariëlle G. van Pampus d, Sicco Scherjon e, Marijke Faas b,e (a IQ Products BV, Research & Development, Groningen, The Netherlands, b University Medical Center Groningen, Division of Medical Biology, Dept. of Pathology and Medical Biology, Groningen, The Netherlands, c University of Groningen, Dept. of Pharmaceutical Analysis, Groningen, The Netherlands, d Onze Lieve Vrouwe Gasthuis, Dept. of Obstetrics and Gynecology, Amsterdam, The Netherlands, e University Medical Center Groningen, Dept. of Obstetrics and Gynecology, Groningen, The Netherlands)