P-Misc-25 Hybrids of sunflower hypocotyl and broad bean guard cell protoplasts by electrofusion

P-Misc-25 Hybrids of sunflower hypocotyl and broad bean guard cell protoplasts by electrofusion

Miscellaneous P-Mist-26 &ND .FUSION OF C~~~LSBYMEAN~OF IiARffOtJ Ibi.;*‘kOVA D.,’ ICOUTSOURISD.,’ and LGuMs s.z ‘BhnaliaI E@teuhg & AppIIed Biophysics...

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Miscellaneous P-Mist-26 &ND .FUSION OF C~~~LSBYMEAN~OF IiARffOtJ Ibi.;*‘kOVA D.,’ ICOUTSOURISD.,’ and LGuMs s.z ‘BhnaliaI E@teuhg & AppIIed BiophysicsLab., NTUA, ArIms (OR), %iochem%q Lab., NCSR, Demokritos,Athens(GR) Pprporn: The purpose of this work is to optimise intactIWa pemxab&ation and fusionand studythe underlyingprocesses. Methods: Rabbit etytbmym (RBCs) were used. !&pare waveand exponcntIaIdecayd.c. pulseswere applied. Bkmic fkkl inducedeffectswerefollowed by dIghI imaging fhiorexence microxopy and fluoreawlce ~scopy.

Metbodsz Guard-ceU-specificgenesand motas were tramsinto sunflower gro ypocoty1protophsta (C3-plant) by electrofhsioa

ReauIts:IntactRBCswetr:fusedandIoadedwith different molecularweight pzubesby appIyiq w waveorcqmmiaJdeuydc.pllfesofrcktivciylow ekctdcfldd stmglb (1 kV/cm)andlong duration(2030 lllsec). CoacImsions: It is oossibk to h&i&se a C4-

COB under these oprimised conditions, efficient loading of Intact red blood ceIIs was succeeded. accompankd by high ceII survival percentages

P-Mist-27 INTERACTION OF ORGANOTIN AND ORGANOLEAD COMPOUNDS WITH MODEL

P~E,4&BIL#&ATION

P-Mix-28

AND FUSION OF

INTACTRRDBLOODCELLSBYMEANS

'OFELECRC~~TION

HARlTOU M,’ YOVA D,’ KOUTSOURIS D, andLGuKAss? ‘Bion~&c8l Eag. & Applied Biophyh Lab., Ni’UA, Mtena (at), %iothday Lab.. NCSR Deardrritor, Atb~ (GR)

s S. SARAPUK J, GABRIELSKA J, IUESZCZYhSKA I& IgADYSulwSKI J, Dqt Phja Biopbys.,Agr.univ., wrodaw (PL)

Purgorc:llle~oftbisworfristooptimise illtactRBcsjl4?aMdollalldfbsiollalld shadythe uuderlying processes. Methods: R&bit eayko@tcs (Rl3Cs) were used.Squarewwaandexpapcatialdecayd.c. pulseswere apphd. Eledric field inducedef%cts were foliowed by digital imaging &onscwna microswpyalldftuorsscgnx spectroscopy. Re!suits:IllmtRBcswaefbsedaadhldcd

with differwt molaxdar weigh probes by applyiogsquarewweoreqoaialdecaydc. pukes of rdathdy low decttic fidd aengtb (1 kV/cm) and long duration (20-30 msec). cordtrdoar:ulldertheseoptimisedcQnditi~ efficient loading of intact nxl blood cells was succeeds acwmpsnied by high cell sti percentages. 221