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P-Misc-29 The mechanism of formation of light scattering function of biological suspension
Mjsccllaneous P-Mist-29 WE MZXXWWM QF I!W&#ATfW. OF LIGHT S~Al-I’ERZb% pzflyctroW‘6F BIOLOGICAL SUSPENSION
LOPATINV.,'SHEPELEVICHN.2
’ Krasnoyarsk StateUniv.(RUS) * Inst.of BiophysicsSB RAS,Rrasnoyarsk (RUS) Purpose: The formation of the light scattering function of biological particles of different structure and shape has been studied theoretically and experimentally. Methods: Mie theory; T-matrix method; Rayleigh, RGD, WKB, AD approximations. Results: The expressions for small angle and integral scattering limctions of homogeneousspheroidal particles are deduced. It is shown that the sole point in the angle scan where extremums are initiated does not depend on particle size. The location of this point is related to aspherity. The expression relating interval between extremums to sizesand shape of particles has been obtained. Conclusions: Algorithms of rigorous analysisof optical characteristics of axisymmetric particles in real time were created. It is proved that the interval between extremums dependson particles size and shape and the contrast on the size, shape and refractive index. This allows substantiating the flow cytometry technique for measuring of parametersof individual particles.
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LOPATINV.,'SHEPELEVICHN.2
’ Krasnoyarsk StateUniv.(RUS) * Inst.of BiophysicsSB RAS,Rrasnoyarsk (RUS) Purpose: The formation of the light scattering function of biological particles of different structure and shape has been studied theoretically and experimentally. Methods: Mie theory; T-matrix method; Rayleigh, RGD, WKB, AD approximations. Results: The expressions for small angle and integral scattering limctions of homogeneousspheroidal particles are deduced. It is shown that the sole point in the angle scan where extremums are initiated does not depend on particle size. The location of this point is related to aspherity. The expression relating interval between extremums to sizesand shape of particles has been obtained. Conclusions: Algorithms of rigorous analysisof optical characteristics of axisymmetric particles in real time were created. It is proved that the interval between extremums dependson particles size and shape and the contrast on the size, shape and refractive index. This allows substantiating the flow cytometry technique for measuring of parametersof individual particles.
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