- Email: [email protected]
P0008 Validating the link between obesity and breast inflammation in women with breast cancer
Abstracts / 50 (2014) e1–e74
the fraction of foci-positive c-H2AX and 53BP1 gastric cancer cells after heating, with a maximum increase occurring 48 h after heating. Non-chemotherapy CD44+ MNK-45 gastric cancer cells exhibited baseline levels of DNA damage assessed by c-H2AX and 53BP1 foci. Increased expression of c-H2AX and 53BP1 in the cancer cells are most likely consistent with increased expression of HSP. Interpretation: These findings suggest that hyperthermia plus chemotherapy can eliminate gastric CSCs resistance to chemotherapy. Mild heat can enhance sensitivity of gastric CSCs to chemotherapy by inhibiting repair of DNA damage.
http://dx.doi.org/10.1016/j.ejca.2014.03.050
P0007 ESTIMATION OF SERUM IRON AND SERUM COPPER IN ORAL PRECANCER, CANCER, AND HEALTHY INDIVIDUALS: A COMPARATIVE STUDY R. Sethuraman. Department of Maxillofacial Diagnostic Sciences, Majmaah University, College of Dentistry, Alzulfi, Saudi Arabia Background: Oral cancer is one of the ten most common cancers in the world and shows a marked geographic difference in occurrence. In the long incubation period between the initiation of carcinogenic habits and development of invasive oral cancer, several well defined oral precancerous lesions such as leukoplakia, oral submucous fibrosis, and erythroplakia occur. The current study is an attempt to suggest a positive role of micronutrients such as serum iron and copper in prevention of oral precancer and cancer. Biochemical assessment of patients with oral premalignant and malignant lesions may help with earlier diagnosis and/or prognosis of these lesions. Methods: A total of 30 patients with oral precancerous lesions and conditions and 30 patients with oral cancer were enrolled for the present study. Thirty healthy individuals within the same age group were included in the control group. Diagnosis was made on the basis of history and clinical examination and was confirmed later histopathologically. Subsequently, the serum of these patients was analysed for iron and copper in the laboratory. Findings: Serum copper levels were increased in the precancerous and cancerous groups when compared with the control group, and this difference was statistically significant (F = 109.524; p < 0.001). Serum iron levels were decreased in the precancerous and cancerous groups when compared with the control group, and the difference was statistically significant (p < 0.001). Interpretation: It can be suggested that biochemical assessment of oral precancerous and cancerous patients may help with earlier diagnosis and/or prognosis of these lesions. This may also help to predict the malignant potential of premalignant lesions and conditions. In turn this puts tremendous responsibility on the shoulders of oral physicians to undertake regular screening and to detect these diseases earlier, which could enhance the health and wellbeing of the population as a whole.
http://dx.doi.org/10.1016/j.ejca.2014.03.051
P0008 VALIDATING THE LINK BETWEEN OBESITY AND BREAST INFLAMMATION IN WOMEN WITH BREAST CANCER
e11
N.M. Iyengar a,b,c,*, P.G. Morris a, X.K. Zhou b, D. Giri a, M. Harbus b, D.J. Falcone b, A. Gucalp a,b, M. Morrow a,b, C.A. Hudis a,b, A.J. Dannenberg b . a Memorial Sloan-Kettering Cancer Center, New York, NY, USA, b Weill Cornell Medical College, New York, NY, USA, c Rockefeller University, New York, NY, USA Background: In post-menopausal women, obesity is a risk factor for hormone sensitive breast cancer. We previously described crown-like structures (CLS), consisting of macrophages surrounding dead adipocytes, in the breast tissues of mice and humans. CLS were associated with increased levels of proinflammatory mediators known to be involved in carcinogenesis. We provided the first evidence of CLS in the human breast (CLS-B), which correlated with increased aromatase expression and activity. Here, we expanded our population to prospectively validate these preliminary findings. Methods: White adipose tissue (WAT) was prospectively collected from women undergoing breast and reconstructive surgery. WAT was subjected to immunohistochemistry for CD68, a macrophage marker, to detect CLS-B by light microscopy. Adipocyte diameter was measured using the Canvas 11 Software. Endpoints were CLS-B presence/absence and CLS-B index (proportion of slides with CLS-B). Findings: From April 2010 to February 2012, WAT (100 mastectomy and five abdominal reconstructions) was obtained from 101 American women; median age 49 years (range 26–80). CLS-B were found in 54 (53%) patients. CLS-B were seen in 9/37 (24%) normal weight patients (body mass index [BMI] < 25), 23/39 (59%) overweight patients (BMI 25–29.9), and 22/25 (88%) obese patients (BMI P 30). Patients with CLS-B had significantly larger adipocyte diameter (106.5 ± 11.5 lm) compared with those without CLS-B (91.5 ± 16.1 lm; p < 0.001). Regular use of non-steroidal anti-inflammatory drugs was protective against CLS-B (p = 0.04). Among 25 patients with bilateral breast WAT and five patients with paired breast and abdominal WAT, concordant CLS-B findings (+/–) were seen in 80%. Interpretation: Findings from this prospective study confirm that CLS-B are associated with BMI and adipocyte size. These results provide a plausible pathophysiological link between obesity and breast cancer. Abdominal WAT may be a surrogate for breast WAT inflammation; biopsies of abdominal subcutaneous WAT are more easily done, and could be performed in future clinical trials that aim to decrease breast WAT inflammation.
http://dx.doi.org/10.1016/j.ejca.2014.03.052
P0009 THE SUM OF LONGEST TUMOUR DIAMETER AS A PREDICTOR FOR SKELETAL METASTASIS IN RENAL CELL CARCINOMA I. Kumar a, G. Kumari a, S. Trivedi b, A.N.D. Dwivedi a,*. a Department of Radiodiagnosis and Imaging, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India, b Department of Urology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India Background: Renal cell carcinoma (RCC) comprises a diverse group of malignant neoplasms that have multifarious histopathological features, cytogenetic abnormalities, and biological behaviour. Approximately a third of RCC patients develop skeletal metastasis with a poor 5-year survival rate of less than 10%. Data explaining how some of these tumours lead to bone metastases sooner than expected are sparse in the existing literature. This study aimed to identify tumour size as a predictor of bone metastases among renal cell carcinoma patients.
the fraction of foci-positive c-H2AX and 53BP1 gastric cancer cells after heating, with a maximum increase occurring 48 h after heating. Non-chemotherapy CD44+ MNK-45 gastric cancer cells exhibited baseline levels of DNA damage assessed by c-H2AX and 53BP1 foci. Increased expression of c-H2AX and 53BP1 in the cancer cells are most likely consistent with increased expression of HSP. Interpretation: These findings suggest that hyperthermia plus chemotherapy can eliminate gastric CSCs resistance to chemotherapy. Mild heat can enhance sensitivity of gastric CSCs to chemotherapy by inhibiting repair of DNA damage.
http://dx.doi.org/10.1016/j.ejca.2014.03.050
P0007 ESTIMATION OF SERUM IRON AND SERUM COPPER IN ORAL PRECANCER, CANCER, AND HEALTHY INDIVIDUALS: A COMPARATIVE STUDY R. Sethuraman. Department of Maxillofacial Diagnostic Sciences, Majmaah University, College of Dentistry, Alzulfi, Saudi Arabia Background: Oral cancer is one of the ten most common cancers in the world and shows a marked geographic difference in occurrence. In the long incubation period between the initiation of carcinogenic habits and development of invasive oral cancer, several well defined oral precancerous lesions such as leukoplakia, oral submucous fibrosis, and erythroplakia occur. The current study is an attempt to suggest a positive role of micronutrients such as serum iron and copper in prevention of oral precancer and cancer. Biochemical assessment of patients with oral premalignant and malignant lesions may help with earlier diagnosis and/or prognosis of these lesions. Methods: A total of 30 patients with oral precancerous lesions and conditions and 30 patients with oral cancer were enrolled for the present study. Thirty healthy individuals within the same age group were included in the control group. Diagnosis was made on the basis of history and clinical examination and was confirmed later histopathologically. Subsequently, the serum of these patients was analysed for iron and copper in the laboratory. Findings: Serum copper levels were increased in the precancerous and cancerous groups when compared with the control group, and this difference was statistically significant (F = 109.524; p < 0.001). Serum iron levels were decreased in the precancerous and cancerous groups when compared with the control group, and the difference was statistically significant (p < 0.001). Interpretation: It can be suggested that biochemical assessment of oral precancerous and cancerous patients may help with earlier diagnosis and/or prognosis of these lesions. This may also help to predict the malignant potential of premalignant lesions and conditions. In turn this puts tremendous responsibility on the shoulders of oral physicians to undertake regular screening and to detect these diseases earlier, which could enhance the health and wellbeing of the population as a whole.
http://dx.doi.org/10.1016/j.ejca.2014.03.051
P0008 VALIDATING THE LINK BETWEEN OBESITY AND BREAST INFLAMMATION IN WOMEN WITH BREAST CANCER
e11
N.M. Iyengar a,b,c,*, P.G. Morris a, X.K. Zhou b, D. Giri a, M. Harbus b, D.J. Falcone b, A. Gucalp a,b, M. Morrow a,b, C.A. Hudis a,b, A.J. Dannenberg b . a Memorial Sloan-Kettering Cancer Center, New York, NY, USA, b Weill Cornell Medical College, New York, NY, USA, c Rockefeller University, New York, NY, USA Background: In post-menopausal women, obesity is a risk factor for hormone sensitive breast cancer. We previously described crown-like structures (CLS), consisting of macrophages surrounding dead adipocytes, in the breast tissues of mice and humans. CLS were associated with increased levels of proinflammatory mediators known to be involved in carcinogenesis. We provided the first evidence of CLS in the human breast (CLS-B), which correlated with increased aromatase expression and activity. Here, we expanded our population to prospectively validate these preliminary findings. Methods: White adipose tissue (WAT) was prospectively collected from women undergoing breast and reconstructive surgery. WAT was subjected to immunohistochemistry for CD68, a macrophage marker, to detect CLS-B by light microscopy. Adipocyte diameter was measured using the Canvas 11 Software. Endpoints were CLS-B presence/absence and CLS-B index (proportion of slides with CLS-B). Findings: From April 2010 to February 2012, WAT (100 mastectomy and five abdominal reconstructions) was obtained from 101 American women; median age 49 years (range 26–80). CLS-B were found in 54 (53%) patients. CLS-B were seen in 9/37 (24%) normal weight patients (body mass index [BMI] < 25), 23/39 (59%) overweight patients (BMI 25–29.9), and 22/25 (88%) obese patients (BMI P 30). Patients with CLS-B had significantly larger adipocyte diameter (106.5 ± 11.5 lm) compared with those without CLS-B (91.5 ± 16.1 lm; p < 0.001). Regular use of non-steroidal anti-inflammatory drugs was protective against CLS-B (p = 0.04). Among 25 patients with bilateral breast WAT and five patients with paired breast and abdominal WAT, concordant CLS-B findings (+/–) were seen in 80%. Interpretation: Findings from this prospective study confirm that CLS-B are associated with BMI and adipocyte size. These results provide a plausible pathophysiological link between obesity and breast cancer. Abdominal WAT may be a surrogate for breast WAT inflammation; biopsies of abdominal subcutaneous WAT are more easily done, and could be performed in future clinical trials that aim to decrease breast WAT inflammation.
http://dx.doi.org/10.1016/j.ejca.2014.03.052
P0009 THE SUM OF LONGEST TUMOUR DIAMETER AS A PREDICTOR FOR SKELETAL METASTASIS IN RENAL CELL CARCINOMA I. Kumar a, G. Kumari a, S. Trivedi b, A.N.D. Dwivedi a,*. a Department of Radiodiagnosis and Imaging, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India, b Department of Urology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India Background: Renal cell carcinoma (RCC) comprises a diverse group of malignant neoplasms that have multifarious histopathological features, cytogenetic abnormalities, and biological behaviour. Approximately a third of RCC patients develop skeletal metastasis with a poor 5-year survival rate of less than 10%. Data explaining how some of these tumours lead to bone metastases sooner than expected are sparse in the existing literature. This study aimed to identify tumour size as a predictor of bone metastases among renal cell carcinoma patients.