- Email: [email protected]
EEG evaluation
Abstracts / Clinical Neurophysiology 128 (2017) e1–e163
may indicate therapeutic selectivity. As such, the correlation between SP and AxS suggests prolonging SP correlates with improved posture, balance and gait (axial symptoms). As PD patients have shortened SP, this correlation could reflect adequate restorative intra-cortical modulation. doi:10.1016/j.clinph.2016.10.150
P023 Motor cortical reorganization and clinical outcomes after stroke: A longitudinal TMS/EEG evaluation—M.C. Pellicciari a,b,*, S. Bonnı` a, M.C. Alex a, P. Viviana a, C. Elias a, G. Koch a,c (a Santa Lucia Foundation, Rome, Non-invasive Brain Stimulation Unit, Rome, Italy, b IRCCS San Giovanni di Dio Fatebenefratelli, Cognitive Neuroscience Section, Brescia, Italy, c Policlinic Tor Vergata, Stroke Unit, Department of Neuroscience, Rome, Italy) ⇑
P022 Evidence that functionalized ferromagnetic microparticles with CD133 antigen decreases glioblastoma multiforme growth in vivo and in vitro by magnetic field induced hyperthermia—J.-R. GarciaMontes a,*, P.-F. Porceddu b, R. Drucker-Colín c, R. Moratalla a, R. Martinez-Murillo a (a Instituto Cajal, Neurobiologia de los ganglios basales, Madrid, Spain, b University of Cagliari, Neuropharmacology, Cagliari, Italy, c Instituto de Fisiología Celular U.N.A.M, Neuropatología Molecular, Mexico, Mexico) ⇑
Corresponding author.
Glioblastoma multiforme (Gb) is considered the most frequent brain tumor. The Gb represents 12–15% of all intracranial tumors and 50–60% of all astrocytic tumors. The poor survival of patients (3–5% in 5 years) along with the marginal improvement produced by drugs, and the recurrence after surgery treatment, indicates that Gb is the most aggressive of the gliomas. Hyperthermia has been successfully applied since late 50’s in many animal models to induce the catabolism of many tumor. The challenge of this work is to show that hyperthermia helps reducing intracranial brain malignant astrocytoma in a reliable mouse model with immunity preserved, as well as to test the safety of the intervention. First, we showed in vitro that ferro-magnetic microparticles (MP) exposed to 200 kA m 1 magnetic strength for 1 h increased temperature. To see if our ‘‘in vitro” results could be replicated ”in vivo” we grafted 20,000 CT-2A tumoral cells in the striatum of C57 mice. We divided the grafted mice (36) in four groups, as follows: 1st, grafted group only (Gb), 2nd grafted group exposed to MFss (Gb + ELFMFS), 3rd grafted group injected with MP (Gb + MP) and 4th grafted group injected with MP and exposed to MFs (Gb + MP + ELFMFS). MP were injected intrastriatally four days after the graft, with 4 ll of ferromagnetic MP at the same coordinates used to graft tumoral cells. The selected groups were exposed to the MF 200 kA m 1 for two hours daily during 7 days while the control groups were exposed to the equipment in off. Mice were sacrificed 1 h. after the magnetic stimulation to study their brains. Further, striatal sections were evaluated for proliferation and astrocyte activation by using Ki67, Iba1 and GFAP markers. We found that TMS reduces microglia 3.5% in the Gb + TMS group and 7.5% in the Gb + MP + TMS group when compared with the Gb group. Microglia increased (7.3%) after intrastriatal administration of MP. We then studied the growth of tumor with Ki67 and observed a 55% reduction of tumor area in mice exposed to TMS versus the control group. In agreement with these results, 30% of mice injected with MP and exposed to MF survived to Gb graft at 21 days. In summary, our study shows the effectiveness of TMS against intracranial neoplasms possibly due to the rise in the temperature of MP after TMS and opens the possibility for further studies in tumor cells.
doi:10.1016/j.clinph.2016.10.151
e21
Corresponding author.
Introduction: Since early days after stroke, the brain undergoes a complex reorganization to allow compensatory mechanisms that promote functional recovery. Characterizing specific neurophysiological markers of motor recovery after stroke could improve clinical decision making. Objectives: To track the time-course of motor cortical reorganization in a stroke patients group, and to individuate the neurophysiological markers associated to clinical outcome. Patients & methods: Ten patients in the sub-acute phase of ischemic subcortical stroke were evaluated within 20 days and after 40, 60 and 180 days after stroke. For each time-point, cortical reactivity and cortical oscillations changes, evoked by 80 single TMS pulses, were assessed over the motor cortex of the affected and unaffected hemisphere, combining TMS-EEG. These measurements were paralleled with motor and clinical evaluations. Repeated measures ANOVA and Friedman test were used to evaluate changes over time of all measures. Results: Our data showed specific cortical oscillatory activity changes in the alpha band, in a specific time point of the longitudinal evaluation only in the affected hemisphere. Stroke patients showed a significant increase in TMS-evoked alpha oscillations, as highlighted by spectral perturbation analysis. Notably, these changes occurred at 60 days after stroke, indicating that crucial mechanisms of cortical reorganization occur in this short-time window. Moreover, a cortical reactivity increase was observed at 40 days after stroke onset in affected hemisphere respect to other times and respect to unaffected hemisphere. These changes coincided with the clinical and behavioural amelioration. Conclusion: For the first time, this study demonstrates the possibility to track longitudinally the motor cortical changes following stroke, by means a multimodal approach. These findings could allow, not only to identify neurophysiological markers of stroke pathophysiology, but also to provide new insight into how and when neuromodulatory interventions could drive neuroplasticity in a functional direction. doi:10.1016/j.clinph.2016.10.152
P024 Hemispheric language dominance measured by rTMS and postoperative course of language function in brain tumor patients— S. Ille a,b,*, N. Kulchytska c, N. Sollmann a,b, R. Wittig a,b, E. Beurskens a,b, V.M. Butenschoen a,b, F. Ringel a, P. Vajkoczy c, B. Meyer a, T. Picht c, S.M. Krieg a,b (a Department of Neurosurgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany, b TUM Neuroimaging Center, Klinikum rechts der Isar, Technische Universität München, Munich, Germany, c Department of Neurosurgery, Charité-Universitätsmedizin Berlin, Berlin, Germany) ⇑
Corresponding author.
may indicate therapeutic selectivity. As such, the correlation between SP and AxS suggests prolonging SP correlates with improved posture, balance and gait (axial symptoms). As PD patients have shortened SP, this correlation could reflect adequate restorative intra-cortical modulation. doi:10.1016/j.clinph.2016.10.150
P023 Motor cortical reorganization and clinical outcomes after stroke: A longitudinal TMS/EEG evaluation—M.C. Pellicciari a,b,*, S. Bonnı` a, M.C. Alex a, P. Viviana a, C. Elias a, G. Koch a,c (a Santa Lucia Foundation, Rome, Non-invasive Brain Stimulation Unit, Rome, Italy, b IRCCS San Giovanni di Dio Fatebenefratelli, Cognitive Neuroscience Section, Brescia, Italy, c Policlinic Tor Vergata, Stroke Unit, Department of Neuroscience, Rome, Italy) ⇑
P022 Evidence that functionalized ferromagnetic microparticles with CD133 antigen decreases glioblastoma multiforme growth in vivo and in vitro by magnetic field induced hyperthermia—J.-R. GarciaMontes a,*, P.-F. Porceddu b, R. Drucker-Colín c, R. Moratalla a, R. Martinez-Murillo a (a Instituto Cajal, Neurobiologia de los ganglios basales, Madrid, Spain, b University of Cagliari, Neuropharmacology, Cagliari, Italy, c Instituto de Fisiología Celular U.N.A.M, Neuropatología Molecular, Mexico, Mexico) ⇑
Corresponding author.
Glioblastoma multiforme (Gb) is considered the most frequent brain tumor. The Gb represents 12–15% of all intracranial tumors and 50–60% of all astrocytic tumors. The poor survival of patients (3–5% in 5 years) along with the marginal improvement produced by drugs, and the recurrence after surgery treatment, indicates that Gb is the most aggressive of the gliomas. Hyperthermia has been successfully applied since late 50’s in many animal models to induce the catabolism of many tumor. The challenge of this work is to show that hyperthermia helps reducing intracranial brain malignant astrocytoma in a reliable mouse model with immunity preserved, as well as to test the safety of the intervention. First, we showed in vitro that ferro-magnetic microparticles (MP) exposed to 200 kA m 1 magnetic strength for 1 h increased temperature. To see if our ‘‘in vitro” results could be replicated ”in vivo” we grafted 20,000 CT-2A tumoral cells in the striatum of C57 mice. We divided the grafted mice (36) in four groups, as follows: 1st, grafted group only (Gb), 2nd grafted group exposed to MFss (Gb + ELFMFS), 3rd grafted group injected with MP (Gb + MP) and 4th grafted group injected with MP and exposed to MFs (Gb + MP + ELFMFS). MP were injected intrastriatally four days after the graft, with 4 ll of ferromagnetic MP at the same coordinates used to graft tumoral cells. The selected groups were exposed to the MF 200 kA m 1 for two hours daily during 7 days while the control groups were exposed to the equipment in off. Mice were sacrificed 1 h. after the magnetic stimulation to study their brains. Further, striatal sections were evaluated for proliferation and astrocyte activation by using Ki67, Iba1 and GFAP markers. We found that TMS reduces microglia 3.5% in the Gb + TMS group and 7.5% in the Gb + MP + TMS group when compared with the Gb group. Microglia increased (7.3%) after intrastriatal administration of MP. We then studied the growth of tumor with Ki67 and observed a 55% reduction of tumor area in mice exposed to TMS versus the control group. In agreement with these results, 30% of mice injected with MP and exposed to MF survived to Gb graft at 21 days. In summary, our study shows the effectiveness of TMS against intracranial neoplasms possibly due to the rise in the temperature of MP after TMS and opens the possibility for further studies in tumor cells.
doi:10.1016/j.clinph.2016.10.151
e21
Corresponding author.
Introduction: Since early days after stroke, the brain undergoes a complex reorganization to allow compensatory mechanisms that promote functional recovery. Characterizing specific neurophysiological markers of motor recovery after stroke could improve clinical decision making. Objectives: To track the time-course of motor cortical reorganization in a stroke patients group, and to individuate the neurophysiological markers associated to clinical outcome. Patients & methods: Ten patients in the sub-acute phase of ischemic subcortical stroke were evaluated within 20 days and after 40, 60 and 180 days after stroke. For each time-point, cortical reactivity and cortical oscillations changes, evoked by 80 single TMS pulses, were assessed over the motor cortex of the affected and unaffected hemisphere, combining TMS-EEG. These measurements were paralleled with motor and clinical evaluations. Repeated measures ANOVA and Friedman test were used to evaluate changes over time of all measures. Results: Our data showed specific cortical oscillatory activity changes in the alpha band, in a specific time point of the longitudinal evaluation only in the affected hemisphere. Stroke patients showed a significant increase in TMS-evoked alpha oscillations, as highlighted by spectral perturbation analysis. Notably, these changes occurred at 60 days after stroke, indicating that crucial mechanisms of cortical reorganization occur in this short-time window. Moreover, a cortical reactivity increase was observed at 40 days after stroke onset in affected hemisphere respect to other times and respect to unaffected hemisphere. These changes coincided with the clinical and behavioural amelioration. Conclusion: For the first time, this study demonstrates the possibility to track longitudinally the motor cortical changes following stroke, by means a multimodal approach. These findings could allow, not only to identify neurophysiological markers of stroke pathophysiology, but also to provide new insight into how and when neuromodulatory interventions could drive neuroplasticity in a functional direction. doi:10.1016/j.clinph.2016.10.152
P024 Hemispheric language dominance measured by rTMS and postoperative course of language function in brain tumor patients— S. Ille a,b,*, N. Kulchytska c, N. Sollmann a,b, R. Wittig a,b, E. Beurskens a,b, V.M. Butenschoen a,b, F. Ringel a, P. Vajkoczy c, B. Meyer a, T. Picht c, S.M. Krieg a,b (a Department of Neurosurgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany, b TUM Neuroimaging Center, Klinikum rechts der Isar, Technische Universität München, Munich, Germany, c Department of Neurosurgery, Charité-Universitätsmedizin Berlin, Berlin, Germany) ⇑
Corresponding author.