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P036 AHA cosmetic products influence on pH values of stratum corneum
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A flare was observed in the last months after cutting wood and lighting an open fire. He denied drug ingestion, but reported topical use of “Vick Vaporub” for many years. Patch tests with GPEDC standard, rural and plant series were performed revealing strong reactions do turpentine and colopbony. During the tests a new flare was documented resulting in hipopigmented areas “in confetti”. The woods were identified as Pinus pinaster and Eucalyptus glob&s.
A few months later, patch-tests were performed again, simultaneously with wood extracts and photoalergen series. All were irradiated at 48 hours. A complete remission of the dermatosis was obtained after the elimination of the sensitizers. I PO33 Analysis of the causes of professional
dermatoses among medical workers
E. Dastychova, Z. Navratilova. I. Dept. Anna
Univ.
Hospital,
Brno,
Czech
of Dermatology, Republic
St.
The aim of this study is the analysis of causes of dermatological diseases based on profession among medical workers. The study was carried out in a region with 2 059 014 inhabitants, where 42 574 people work in health service. 200 professional dermatoses were identified from 1976 till 1995, i.e. 10.3% of all professional diseases in health service. The frequency of distribution due to the specific workers’ categories and to their speciahsation was followed. The most frequently identified dermatoses were contact eczema (81.5%), infectious dermatological affection (1 l%), chronical radiodermatitis (4.5%), and irritative dermatitis (3%). The most frequent causes of contact eczema were found to be disinfectants, rubber, drugs, and acrylats. The result of this study are important for: a) choice of speciahsation b) changing the procedures of work c) selection of protective agents. 0 PO34 Permanent recurrent eruptions due to
Cnidaria. Two cases report
F. Kokelj, C. Plozzer, P. Torsello, G. Lavaroni. Institute Dermatology,
University
of Trieste,
of
Italy
The possibility of permanent recurrent cutaneous eruptions after Cnidaria stings has been already reported; their aethology is usually unknown. L.C., 19 year-old man, stung by a Pelagia Noctiluca while he was swimming in the waters of the Gulf of Biscaglia, presented a painful erythematous vesicular and oedematous eruption at his right wrist. The lesion improved spontaneously leaving an hypertrophic scar resistent to the different treatments (cryotherapy, oral and topical steroids). Only a surgical excision was able to resolve his problem. In July 1996 a 17 year-old girl has been stung on her right forearm by an Anemonia Sulcata while she was swimming along the Trieste coast. The sting caused a burning, localized erythematous and vesicular eruption in the affected area that spontaneously improved in about one month. Erythema and vescicles reappeared in the same site three and seven months later after mosquito stings. In both these occasions topical steroids healed the lesions.
S161
Dermatitis
IPO35
Maximization test for identification of allergenic and photoallergenic potential using SDS instead of UVB
I. Tausch, B. Hughes-Formella, for Dermatological Germany
Research
J. Gassmiiller. BioSkin and Development
Ltd.,
Institute Hamburg,
A relevant statement of allergenic and photoallergenic potential of topical preparations. using the classic Draize-Shelanski insult test requires a minimum of 200 subjects, whereby recognition of slight allergenic potential is still not ensured. The maximation test using pretreatment with 5% sodium dodecyl sulfate (SDS) or 3 MED UVB to impair the skin prior to application of test preparations was introduced by Kligmann. This allows improved penetration of preparations through the dermal barrier, increasing exposition to immune competent cells. An advantage of maximization is testing on fewer subjects by a simultaneous increase in the sensitization rate. In recent years the maximization test with UVB has been favored. In light of current knowledge of dermal damage and well documented down-regulation of immune response following repeated irradiation, the suitability of this model is questionable. Therefore, we prefer a modification of the procedure using pretreatment with 0.2-0.5% SDS to test sites on the backs of light-skinned subjects during the induction phase. In this way the desired barrier insult is achieved without persistent skin damage. I PO36 AHA cosmetic products influence on pH
values of stratum corneum
B. Vincetic, J. LipozenW ‘, S. Bolanza-Bumber*, A. Basta-JuzbaSiC ’ “Saponia”, Cosmetic industry, ‘Department *University
of Dermatovenerology, Zagreb Clinical Schoot of Medicine Zagreb, Croatia
Z. BukviC ‘, Osijek; Hospital:
For cosmetics based on alpha hydroxy acids (AHA), the pHvalue of the skin is one of the most important indicators of their efficacy, because of his direct influence on the pH-value of the skin. Included in the study were 60 subjects (two groups of 30 women aged 25-.58), who used creams with different pH-values. Both groups were using the placebo sample (pH 6.6) on the right forearm, and on the left forearm different active creams: cream C with pH 2.7 and cream D with pH 3.7, respectively. Concentration of active AHA-complex in both creams was 5.0%. Measurements of the skin pH-values in vivo prior to, 30 minutes after, and 1,2, 3, and 6 weeks after application showed a reduction of pH-value in the surface layer of stratum comeum in comparison with the placebo sample. The regular use of AHA-based creams for 6 weeks causes average reduction of skin pH for nearly 0.5 pH-value unit for cream D, and nearly 1.O pH-value unit for cream C and doesn’t go back to the value measured before application of the examined active creams.
A flare was observed in the last months after cutting wood and lighting an open fire. He denied drug ingestion, but reported topical use of “Vick Vaporub” for many years. Patch tests with GPEDC standard, rural and plant series were performed revealing strong reactions do turpentine and colopbony. During the tests a new flare was documented resulting in hipopigmented areas “in confetti”. The woods were identified as Pinus pinaster and Eucalyptus glob&s.
A few months later, patch-tests were performed again, simultaneously with wood extracts and photoalergen series. All were irradiated at 48 hours. A complete remission of the dermatosis was obtained after the elimination of the sensitizers. I PO33 Analysis of the causes of professional
dermatoses among medical workers
E. Dastychova, Z. Navratilova. I. Dept. Anna
Univ.
Hospital,
Brno,
Czech
of Dermatology, Republic
St.
The aim of this study is the analysis of causes of dermatological diseases based on profession among medical workers. The study was carried out in a region with 2 059 014 inhabitants, where 42 574 people work in health service. 200 professional dermatoses were identified from 1976 till 1995, i.e. 10.3% of all professional diseases in health service. The frequency of distribution due to the specific workers’ categories and to their speciahsation was followed. The most frequently identified dermatoses were contact eczema (81.5%), infectious dermatological affection (1 l%), chronical radiodermatitis (4.5%), and irritative dermatitis (3%). The most frequent causes of contact eczema were found to be disinfectants, rubber, drugs, and acrylats. The result of this study are important for: a) choice of speciahsation b) changing the procedures of work c) selection of protective agents. 0 PO34 Permanent recurrent eruptions due to
Cnidaria. Two cases report
F. Kokelj, C. Plozzer, P. Torsello, G. Lavaroni. Institute Dermatology,
University
of Trieste,
of
Italy
The possibility of permanent recurrent cutaneous eruptions after Cnidaria stings has been already reported; their aethology is usually unknown. L.C., 19 year-old man, stung by a Pelagia Noctiluca while he was swimming in the waters of the Gulf of Biscaglia, presented a painful erythematous vesicular and oedematous eruption at his right wrist. The lesion improved spontaneously leaving an hypertrophic scar resistent to the different treatments (cryotherapy, oral and topical steroids). Only a surgical excision was able to resolve his problem. In July 1996 a 17 year-old girl has been stung on her right forearm by an Anemonia Sulcata while she was swimming along the Trieste coast. The sting caused a burning, localized erythematous and vesicular eruption in the affected area that spontaneously improved in about one month. Erythema and vescicles reappeared in the same site three and seven months later after mosquito stings. In both these occasions topical steroids healed the lesions.
S161
Dermatitis
IPO35
Maximization test for identification of allergenic and photoallergenic potential using SDS instead of UVB
I. Tausch, B. Hughes-Formella, for Dermatological Germany
Research
J. Gassmiiller. BioSkin and Development
Ltd.,
Institute Hamburg,
A relevant statement of allergenic and photoallergenic potential of topical preparations. using the classic Draize-Shelanski insult test requires a minimum of 200 subjects, whereby recognition of slight allergenic potential is still not ensured. The maximation test using pretreatment with 5% sodium dodecyl sulfate (SDS) or 3 MED UVB to impair the skin prior to application of test preparations was introduced by Kligmann. This allows improved penetration of preparations through the dermal barrier, increasing exposition to immune competent cells. An advantage of maximization is testing on fewer subjects by a simultaneous increase in the sensitization rate. In recent years the maximization test with UVB has been favored. In light of current knowledge of dermal damage and well documented down-regulation of immune response following repeated irradiation, the suitability of this model is questionable. Therefore, we prefer a modification of the procedure using pretreatment with 0.2-0.5% SDS to test sites on the backs of light-skinned subjects during the induction phase. In this way the desired barrier insult is achieved without persistent skin damage. I PO36 AHA cosmetic products influence on pH
values of stratum corneum
B. Vincetic, J. LipozenW ‘, S. Bolanza-Bumber*, A. Basta-JuzbaSiC ’ “Saponia”, Cosmetic industry, ‘Department *University
of Dermatovenerology, Zagreb Clinical Schoot of Medicine Zagreb, Croatia
Z. BukviC ‘, Osijek; Hospital:
For cosmetics based on alpha hydroxy acids (AHA), the pHvalue of the skin is one of the most important indicators of their efficacy, because of his direct influence on the pH-value of the skin. Included in the study were 60 subjects (two groups of 30 women aged 25-.58), who used creams with different pH-values. Both groups were using the placebo sample (pH 6.6) on the right forearm, and on the left forearm different active creams: cream C with pH 2.7 and cream D with pH 3.7, respectively. Concentration of active AHA-complex in both creams was 5.0%. Measurements of the skin pH-values in vivo prior to, 30 minutes after, and 1,2, 3, and 6 weeks after application showed a reduction of pH-value in the surface layer of stratum comeum in comparison with the placebo sample. The regular use of AHA-based creams for 6 weeks causes average reduction of skin pH for nearly 0.5 pH-value unit for cream D, and nearly 1.O pH-value unit for cream C and doesn’t go back to the value measured before application of the examined active creams.