- Email: [email protected]
P059 “Multivitamin allergy” caused by ethoxyquin
Posters -Dermatitis bath or a disperion oil bath an influence on the aforementioned parameters? The research results presented here, show a dehydrative effect (Capacitance, Comeometer CM 820”) of water baths and oil baths with a high content of a non-ionogenic tenside after standardisated washing. Baths of this composition lead to an increase of the transepidermal water loss (TEWL; Tewameter TM 210”) in the sense of a slight barriere damage. With oil baths containing a small share of the non-ionogenic tenside and even more with spreading oil baths an increase of the hydratation of the stratum comeum and a decrease of the transepidennal water loss in the sense of an occlusive effect can be proven, by contrast. ElPO57
Antigen specific immunoglobulin E to plant pollens in the plasma of patients with atopic dermatitis
W. Silny, M. Czamecka-Operacz, D. Siatecka, M. Piotrowski, P. Silny. Department of Dermatology, University School of Medicine, Poznan, Poland The study involved 103 patients with atopic dermatitis (AD) between 4 and 66 years of age. The level of antigen specific IgE (asIgE) to 18 grass, weed, bush and tree pollens was determined using the CAP FEIA (Pharmacia, Uppsala, Sweden) The presence of the above asIgE was found in 55 patients (53.4%). The most commonly present hypersensitivities were to timothy, mugwort, grey alder, common silver birch and hazel allergens. The authors believe, that the pathomechanism of the development of skin lesions in a topic dermatitis is governed to a large degree by hypersensitivity to plant pollens, and suggest that precise determination of a IgE to these allergens in patients blood sera should form the basis of the construction of the vaccine used in their immunotherapy. I PO58 Langerhans cells (CDla+) and TGF-B in
chronically photoexposed skin of aged subjects treated with glycolic acid
C. Comacchi, I. Ghersetich, P. Teofoli ’ , T. Lotti ‘. Department of Dermatology, University of Florence; ‘IDI, IRCCS, Rome; 2Department of Dermatology, University of Siena, Italy Glycolic acid (GA) is the most commonly used alpha hydroxy acid for cosmetological purposes. It is considered a versatile superficial peeling agent when used at 50% to 70% concentrations. GA works on the epidermis by decreasing comeocyte adhesion (1). This property may permit efficient clinical control of different conditions, i.e. dry skin, ichthyosis, follicular hyperkeratosis, and others characterized by retention of stratum comeum. Recently GA has been largely employed in the treatment of photo-aged skin although the exact mechanism of action is not clear (2). Langerhans cells are a well known group of skin elements devoted to antigen presentation and to immune-surveillance which decrease linearly with skin aging. We performed an immunohistochemical study on the photoaged skin of five subjects treated with glycolic acid to evaluate the modification of Langerhans cells and the cutaneous levels of TGF-j3. Our study showed in GA treated areas a strong increase of Langerhans cell levels (CDla+) as well as of
S167
TGF-,!J, which is involved in fibroblast activation. On the basis of these preliminary data we suggest that glycolic acid may act by modifying the cutaneous cytokine pattern, with consequent fibroblast activation and increase of Langerhans cell levels. References
[l] EJ Van Scott, RJ Yu. J Am Acad Dermatol 11: 867-879, 1984. [2] I Ghersetich, T Lotti. Abstract Book 4th EADV Congress, Brussels, October 10-15, 1995, p. 118. ElPO59 “Multivitamin allergy” caused by ethoxyquin K. Alanko, R. Jolanki, T. Estlander, L. Kanerva. Section of Dermatology, Finnish Institute of Occupational Health, Helsinki, Finland A 43-year-old man had worked in fur animal fodder manufacture for 17 years when he developed an extensive eczematous rash. He connected the rash to the adding of a multivitamin preparation in powder form to the fodder. The rash was very therapy-resistant, and it relapsed four times within a single week at work. Six patch test sessions were performed. The vitamin mixture gave a strong (+++) reaction as is, and a weak (+) reaction at 0.5% pet. Positive test reactions were obtained with several components of the vitamin mixture. With purer preparations provided by the vitamin manufacturer, allergic reactions were seen only with vitamin A and vitamin D3 preparations, both containing ethoxyquin as an antioxidant. Ethoxyquin 0.5% (Chemotechnique Diagnostics) showed a strong allergic reaction, and a dilution series was positive (++) still at 0.0005% pet. Tests with pure crystalline vitamins A and D3 gave negative results. In conclusion, the ‘vitamin allergy’ of our patient was due to ethoxyquin. Ethoxyquin is used in animal fodder and animal vitamins but not in human vitamins. It should be kept in mind as a potential skin sensitizer of animal fodder handlers. I PO60 Purity analysis of acrylate compounds L. Kanerva, M.-L. Henriks-Eckerman, R. Jolanki. Finnish Institute of Occupational Health, Helsinki and Turku, Finland Objective: We wanted to study how pure commercial (meth) acrylic patch test substances are. We also analyzed acrylic compounds that have caused allergic contact dermatitis, such as dental composites and bonding materials, adhesives and UV-cured lacquers. Methods: The purity was determined by gas chromatography. The impurities were identified by mass spectrometry in both electron impact and chemical ionization mode. The same methods were used to analyze various products containing acrylics. The results were compared to data given in material safety data sheets (MSDS) and product declarations (PD). Results: The analyzed patch test dimethacrylates were very pure (97-99%). The purity of the diacrylates were 81-91%, but tripropyleneglycol diacrylate (TPGDA; 8 1% pure) contained as much as 13% tripropyleneglycol monoacrylate. The purities of the triacrylates were 82-86%. All di- and triacrylates contained l-13% of the corresponding hydroxyacrylates. Dental acrylics contained up to 37% of undeclared triethylene glycol dimethacrylate. 7.6% undeclared BIS-GMA was present in a
Antigen specific immunoglobulin E to plant pollens in the plasma of patients with atopic dermatitis
W. Silny, M. Czamecka-Operacz, D. Siatecka, M. Piotrowski, P. Silny. Department of Dermatology, University School of Medicine, Poznan, Poland The study involved 103 patients with atopic dermatitis (AD) between 4 and 66 years of age. The level of antigen specific IgE (asIgE) to 18 grass, weed, bush and tree pollens was determined using the CAP FEIA (Pharmacia, Uppsala, Sweden) The presence of the above asIgE was found in 55 patients (53.4%). The most commonly present hypersensitivities were to timothy, mugwort, grey alder, common silver birch and hazel allergens. The authors believe, that the pathomechanism of the development of skin lesions in a topic dermatitis is governed to a large degree by hypersensitivity to plant pollens, and suggest that precise determination of a IgE to these allergens in patients blood sera should form the basis of the construction of the vaccine used in their immunotherapy. I PO58 Langerhans cells (CDla+) and TGF-B in
chronically photoexposed skin of aged subjects treated with glycolic acid
C. Comacchi, I. Ghersetich, P. Teofoli ’ , T. Lotti ‘. Department of Dermatology, University of Florence; ‘IDI, IRCCS, Rome; 2Department of Dermatology, University of Siena, Italy Glycolic acid (GA) is the most commonly used alpha hydroxy acid for cosmetological purposes. It is considered a versatile superficial peeling agent when used at 50% to 70% concentrations. GA works on the epidermis by decreasing comeocyte adhesion (1). This property may permit efficient clinical control of different conditions, i.e. dry skin, ichthyosis, follicular hyperkeratosis, and others characterized by retention of stratum comeum. Recently GA has been largely employed in the treatment of photo-aged skin although the exact mechanism of action is not clear (2). Langerhans cells are a well known group of skin elements devoted to antigen presentation and to immune-surveillance which decrease linearly with skin aging. We performed an immunohistochemical study on the photoaged skin of five subjects treated with glycolic acid to evaluate the modification of Langerhans cells and the cutaneous levels of TGF-j3. Our study showed in GA treated areas a strong increase of Langerhans cell levels (CDla+) as well as of
S167
TGF-,!J, which is involved in fibroblast activation. On the basis of these preliminary data we suggest that glycolic acid may act by modifying the cutaneous cytokine pattern, with consequent fibroblast activation and increase of Langerhans cell levels. References
[l] EJ Van Scott, RJ Yu. J Am Acad Dermatol 11: 867-879, 1984. [2] I Ghersetich, T Lotti. Abstract Book 4th EADV Congress, Brussels, October 10-15, 1995, p. 118. ElPO59 “Multivitamin allergy” caused by ethoxyquin K. Alanko, R. Jolanki, T. Estlander, L. Kanerva. Section of Dermatology, Finnish Institute of Occupational Health, Helsinki, Finland A 43-year-old man had worked in fur animal fodder manufacture for 17 years when he developed an extensive eczematous rash. He connected the rash to the adding of a multivitamin preparation in powder form to the fodder. The rash was very therapy-resistant, and it relapsed four times within a single week at work. Six patch test sessions were performed. The vitamin mixture gave a strong (+++) reaction as is, and a weak (+) reaction at 0.5% pet. Positive test reactions were obtained with several components of the vitamin mixture. With purer preparations provided by the vitamin manufacturer, allergic reactions were seen only with vitamin A and vitamin D3 preparations, both containing ethoxyquin as an antioxidant. Ethoxyquin 0.5% (Chemotechnique Diagnostics) showed a strong allergic reaction, and a dilution series was positive (++) still at 0.0005% pet. Tests with pure crystalline vitamins A and D3 gave negative results. In conclusion, the ‘vitamin allergy’ of our patient was due to ethoxyquin. Ethoxyquin is used in animal fodder and animal vitamins but not in human vitamins. It should be kept in mind as a potential skin sensitizer of animal fodder handlers. I PO60 Purity analysis of acrylate compounds L. Kanerva, M.-L. Henriks-Eckerman, R. Jolanki. Finnish Institute of Occupational Health, Helsinki and Turku, Finland Objective: We wanted to study how pure commercial (meth) acrylic patch test substances are. We also analyzed acrylic compounds that have caused allergic contact dermatitis, such as dental composites and bonding materials, adhesives and UV-cured lacquers. Methods: The purity was determined by gas chromatography. The impurities were identified by mass spectrometry in both electron impact and chemical ionization mode. The same methods were used to analyze various products containing acrylics. The results were compared to data given in material safety data sheets (MSDS) and product declarations (PD). Results: The analyzed patch test dimethacrylates were very pure (97-99%). The purity of the diacrylates were 81-91%, but tripropyleneglycol diacrylate (TPGDA; 8 1% pure) contained as much as 13% tripropyleneglycol monoacrylate. The purities of the triacrylates were 82-86%. All di- and triacrylates contained l-13% of the corresponding hydroxyacrylates. Dental acrylics contained up to 37% of undeclared triethylene glycol dimethacrylate. 7.6% undeclared BIS-GMA was present in a