P091 TOPICAL NUCLEOTIDES IMPROVE WOUND HEALING IN RAPAMYCIN TREATED MICE

Hormones, mediators and immunity 2 were 3.50±0.37 g/dL and 3.10±0.42 g/dL, respectively, showing decrease in both groups, however, significant (p < 0.05) inhibition of decrease in albumin was noted in the group G compared with that of the group O. Further BUN, 3 POD, they were 47.0±12.50 g/dL and 26.7±7.29 g/ dL, respectively, showing a significant increase (p < 0.05) in the group G. However, on 7 POD, decreased to 33.8±18.80 g/dL in the group G and to 14.7±2.87 g/dL in the group O; no significant differences were seen between the two groups. As for growth hormone, 7 POD, they were 1.37±1.51 ng/mL and 0.96±1.06 ng/mL, respectively, showing a significant difference (p < 0.05) between the two groups. With regard to IL-6, there were no changes in the group G during the observation period, while in the group O, it started increasing significantly (p < 0.01) on day 1 POD, and even on day 7, an increased status in comparison to pre-operative level continued. Conclusion: We evaluated clinical usefulness of high dose glutamine in patients who underwent cardiovascular surgery. In Japan, 10 g/day of glutamine is administered typically at present, however the same efficacy as reported in other countries has not been found. When it comes to dosing of glutamine under invasive conditions, it was indicated that the conventional dose of 10 g is insufficient, and 0.4 to 0.6 g/BW is effective for Japanese patients as well. Disclosure of Interest: None declared

Hormones, mediators and immunity 2 P091 Outstanding abstract TOPICAL NUCLEOTIDES IMPROVE WOUND HEALING IN RAPAMYCIN TREATED MICE Z.T. Csapo1 , A.D. Kulkarni2 , K. Yamauchi2 , D.Y. Liu1 , C.T. Van Buren1 . 1 Surgery, Division of Immunology and Organ Transplantation, 2 Surgery, University of Texas Health Science Center, Medical School at Houston., Houston, USA Rationale: The immunosuppressant rapamycin (rapa) impairs wound healing possibly by blocking angiogenesis. On a full thickness skin wound animal model we ran a dose response test to determine the dose of rapamycin in mice delaying wound healing. Using the effective dose the aim of our study was to observe if mixed RNA topical can decrease this delay in wound healing in the same animal model. Methods: 8 mg/kg of rapa was gavage administered every other day to Balb/c mice. After 2 weeks of rapa therapy, an 8 mm full thickness flank punch wound was performed in the mice. 10 mice/group were treated topically daily with one of four formulations: KY jelly (control); 0.025% nucleotides (NT); 0.25% NT; 2.5% NT. The NT formula was a mono and dinucleotide mixture provided by ZhenAo Group (China), with the formulation representing the content by weight of NT in the topical application. Every other day digital pictures were taken and wound size measured. T-tests were used to compare groups for rate of healing.

67 Results: 0.025% NT mixture failed to enhance wound healing in rapa treated mice. 0.25% and 2.5% mixed NT groups had more rapid healing of wounds after day 2, with significant healing of the wound observed on day 4 in 0.25% group (p < 0.05) and day 6 in 2.5% NT group (p < 0.02) compared with the control group. This difference in healing in the NT treated groups persisted through day 10. All wounds in all groups were healed by day 14. Conclusion: This is the first observation that topical therapy with NT can favorably influence wound healing even in animals treated with rapamycin. Studies in other species need to determine the clinical applicability of this therapy. Disclosure of Interest: None declared

P092 Outstanding abstract ENDOCRINE REGULATION OF THE APPETITE: EFFECT OF BARIATRIC SURGERY ON THE BALANCE BETWEEN OREXIGENIC (GHRELIN) AND ANOREXIGENIC (PEPTIDE YY) GUT HORMONES J.M. Garcia-Almeida1 , F. Vilchez1 , L. Garrido-Sanchez2 , S. Garcia-Serrano3 , J.A. Garcia-Arnes4 , J. GarciaAleman1 , M. Gonzalo4 , G. Rojo-Martinez5 , J. Rivas-Marin6 , J.L. Gallego-Perales7 , F. Soriguer-Escofet4 , E. Garcia-Fuentes5 . 1 Endocrinology, Hospital Virgen de la Victoria, Malaga, 2 Ciber Fisiopatolog´ ıa Obesidad y Nutrici´ on (CB06/03/0018), Instituto de Salud Carlos olicas III, Malaga, 3 Ciber Diabetes y Enfermedades Metab´ (CB07/08/0019)., Instituto de Salud Carlos III, 4 Endocrinology, Hospital Carlos Haya, 5 Fundacion IMABIS, Instituto de Salud Carlos III, 6 Surgery, Hospital Virgen de la Victoria, 7 Surgery, Hospital Carlos Haya, Malaga, Spain Rationale: The balance between orexigenic and anorexigenic gut hormones suggests an important role for endocrine cells of the gastrointestinal tract in the short-term regulation of food intake. Various studies have shown that serum levels of Peptide YY (PYY) and ghrelin change after bariatric surgery. We undertook a simultaneous evaluation of the serum changes in PYY and ghrelin depending on the specific type of bariatric surgery. Methods: Total PYY and ghrelin were analysed in 29 healthy persons and in 51 morbidly obese persons undergoing open biliopancreatic diversion of Scopinaro (BPD) (n = 37) or laparoscopic Roux-en-Y gastric bypass (RYGB) (n = 14). Control

PYY (pg/mL) Ghrelin (pg/mL)

324.8 ±146.5 1416.0 ±520.2

MO Presurgery

MO Postsurgery

BPD

GB

BPD

GB

260.3 ±100.8* 740.2 ±220.2*

190.6 ±139.9* 734.3 ±286.1**

1075.5 ±657.4 779.0 ±210.7

382.4 ±212.9*** 1137.6 ±316.1**

Data are presented as Mean±S.D. *P < 0.05; **P < 0.01; ***P < 0.001: significant differences.

Results: Bariatric surgery resulted in a significant increase in PYY levels (236.9±186.7 pg/mL vs. 874.9±457.2 pg/mL, p < 0.001). However, the increase was significantly greater in those patients who underwent BPD compared with RYGB (p < 0.001). After bariatric surgery,