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P1.02. Immunohistochemical expression of Cyclin D1 and Ki67 in salivary gland mucoepidermoid carcinomas
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Oral Oncology Supplement journal homepage: www.elsevier.com/locate/oraloncology
Poster session I: Salivary gland tumors, odontogenic tumors, bisphosphonates osteonecrosis, mesenchymal tumors, case reports, quality of life, premalignant lesions P1.01. Tumor-associated macrophage correlated with angiogenesis and progression of mucoepidermoid carcinoma of salivary glands Y.-S. Shieh a,*, S.-G. Shiah b a
School of Dentistry, National Defense Medical Center, Taiwan Institute of Cancer Research, National Health Research Institutes, Taiwan b
Background: There is considerable controversy as to whether tumor-associated macrophage (TAM) promote or inhibit tumor progression. Present study examined the clinicopathologic significance of TAM and its association with tumor angiogenesis, cell proliferation and apoptosis in mucoepidermoid carcinoma (MEC). The potential effect of TAM on cancer cells also was investigated. Methods: CD68, CD34, Ki-67, and vascular endothelial growth factor (VEGF)-A immunohistochemical staining and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) were evaluated in 41 MEC patients. The biologic effect of macrophage on MEC cancer cells was examined in a co-culture system. Results: Proliferation index (PI) and apoptotic index (AI) were 11.7 ± 5.9% and 4.1 ± 2.3% in cancers, respectively. Significant correlation between PI and tumor grade, PI/AI ratio with tumor size and stage were noted. The distribution of intratumoral TAM and microvessel density (MVD) was heterogeneous. TAM count associated strongly with tumor size, grading, and staging of MEC. Higher intratumoral MVD was observed frequently in patients with large, intermediate/high grade, and advanced stages tumors. VEGF-A expression correlated significantly with tumor size and stage. MVD count closely associated with TAM count and VEGF-A expression. While cancer cells were co-cultured with macrophage, increased migration and invasion ability, elevated VEGF-A expression, and enhanced tube-formation of endothelial cells were observed. Conclusion: Our data suggest the tumor-promoting role of TAM in MEC. Furthermore, the TAM, intratumoral MVD, and the PI/AI ratio are potentially useful markers of progression in patients with MEC. doi:10.1016/j.oos.2009.06.287
Introduction: The purpose of this study is to investigate the expression of Cyclin D1 and Ki67 in salivary gland mucoepidermoid carcinoma (MEC) and assess their roles in the carcinogenesis and prognosis of MEC. Methods: Tumor specimens from 34 MEC patients were obtained. And 10 cases of normal salivary glands were used as controls. The tissue specimens had been fixed in neutral-buffered formalin and embedded in paraffin-wax. Immunohistochemical staining was performed on 4-lm thick paraffin sections using the streptavidin– biotin-complex technique. Data were analyzed using SPSS version 11.5 for windows. Results: Cyclin D1 expression could hardly be observed in normal salivary glands, but 73.53% of MEC showed positive staining. Ki67 expression could be recognized in 85.29% of MEC cases, compared to the positive expression in only 10% of normal controls. By statistical analysis, we found that Cyclin D1 and Ki67 showed significant higher expression in MEC than in normal salivary glands. And their expression significantly increased in high grade MEC, compared to low grade MEC. Significant differences were found in expressions of Cyclin D1 and Ki67 between recurrent and non-recurrent MEC. Similarly, we found significant differences in Cyclin D1 and Ki67 expressions between metastatic and non-metastatic MEC. We examined the correlation of Cyclin D1 expression with Ki67 expression in MEC. A significant correlation between them was observed. Discussion: In summary, the experimental data presented here showed that the expression of Cyclin D1 might play a certain role in the development and progression of MEC. Cyclin D1 and Ki67 expression also related with the recurrence and metastasis of MEC. Combined analysis of the expression of Cyclin D1 and Ki67 might be useful to make the prognosis of MEC. Granted by National Natural Science Foundation of China (30700955). doi:10.1016/j.oos.2009.06.288
P1.03. Immunohistochemical expression of cytokeratin profile in salivary gland acinic cell carcinoma T. Liu a,*, L. Wang b, E. Zhu a, L. Gao a a b
P1.02. Immunohistochemical expression of Cyclin D1 and Ki67 in salivary gland mucoepidermoid carcinomas T. Liu a,*, N. Xu a, E. Zhu a, L. Wang b a b
Dalian Medical University, China Northeastern University, China
Dalian Medical University, China Northeastern University, China
Introduction: The purpose of this study is to investigate the cytokeratin (CK) profile in salivary gland acinic cell carcinoma (ACC), then propose a rational hypothesis for its histogenesis. Methods: Tumor specimens from 18 ACC patients were obtained. The tissue specimens had been fixed in neutral-buffered formalin
Poster Abstracts Oral AbstractsPoster ListOrals ListPan. Disc. & Symp. Abs.Keynote Abs.Keynote Bios.ProgramIAOOWelcomeCommittee Listings
Oral Oncology Supplement 3 (2009) 123–161
Oral Oncology Supplement journal homepage: www.elsevier.com/locate/oraloncology
Poster session I: Salivary gland tumors, odontogenic tumors, bisphosphonates osteonecrosis, mesenchymal tumors, case reports, quality of life, premalignant lesions P1.01. Tumor-associated macrophage correlated with angiogenesis and progression of mucoepidermoid carcinoma of salivary glands Y.-S. Shieh a,*, S.-G. Shiah b a
School of Dentistry, National Defense Medical Center, Taiwan Institute of Cancer Research, National Health Research Institutes, Taiwan b
Background: There is considerable controversy as to whether tumor-associated macrophage (TAM) promote or inhibit tumor progression. Present study examined the clinicopathologic significance of TAM and its association with tumor angiogenesis, cell proliferation and apoptosis in mucoepidermoid carcinoma (MEC). The potential effect of TAM on cancer cells also was investigated. Methods: CD68, CD34, Ki-67, and vascular endothelial growth factor (VEGF)-A immunohistochemical staining and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) were evaluated in 41 MEC patients. The biologic effect of macrophage on MEC cancer cells was examined in a co-culture system. Results: Proliferation index (PI) and apoptotic index (AI) were 11.7 ± 5.9% and 4.1 ± 2.3% in cancers, respectively. Significant correlation between PI and tumor grade, PI/AI ratio with tumor size and stage were noted. The distribution of intratumoral TAM and microvessel density (MVD) was heterogeneous. TAM count associated strongly with tumor size, grading, and staging of MEC. Higher intratumoral MVD was observed frequently in patients with large, intermediate/high grade, and advanced stages tumors. VEGF-A expression correlated significantly with tumor size and stage. MVD count closely associated with TAM count and VEGF-A expression. While cancer cells were co-cultured with macrophage, increased migration and invasion ability, elevated VEGF-A expression, and enhanced tube-formation of endothelial cells were observed. Conclusion: Our data suggest the tumor-promoting role of TAM in MEC. Furthermore, the TAM, intratumoral MVD, and the PI/AI ratio are potentially useful markers of progression in patients with MEC. doi:10.1016/j.oos.2009.06.287
Introduction: The purpose of this study is to investigate the expression of Cyclin D1 and Ki67 in salivary gland mucoepidermoid carcinoma (MEC) and assess their roles in the carcinogenesis and prognosis of MEC. Methods: Tumor specimens from 34 MEC patients were obtained. And 10 cases of normal salivary glands were used as controls. The tissue specimens had been fixed in neutral-buffered formalin and embedded in paraffin-wax. Immunohistochemical staining was performed on 4-lm thick paraffin sections using the streptavidin– biotin-complex technique. Data were analyzed using SPSS version 11.5 for windows. Results: Cyclin D1 expression could hardly be observed in normal salivary glands, but 73.53% of MEC showed positive staining. Ki67 expression could be recognized in 85.29% of MEC cases, compared to the positive expression in only 10% of normal controls. By statistical analysis, we found that Cyclin D1 and Ki67 showed significant higher expression in MEC than in normal salivary glands. And their expression significantly increased in high grade MEC, compared to low grade MEC. Significant differences were found in expressions of Cyclin D1 and Ki67 between recurrent and non-recurrent MEC. Similarly, we found significant differences in Cyclin D1 and Ki67 expressions between metastatic and non-metastatic MEC. We examined the correlation of Cyclin D1 expression with Ki67 expression in MEC. A significant correlation between them was observed. Discussion: In summary, the experimental data presented here showed that the expression of Cyclin D1 might play a certain role in the development and progression of MEC. Cyclin D1 and Ki67 expression also related with the recurrence and metastasis of MEC. Combined analysis of the expression of Cyclin D1 and Ki67 might be useful to make the prognosis of MEC. Granted by National Natural Science Foundation of China (30700955). doi:10.1016/j.oos.2009.06.288
P1.03. Immunohistochemical expression of cytokeratin profile in salivary gland acinic cell carcinoma T. Liu a,*, L. Wang b, E. Zhu a, L. Gao a a b
P1.02. Immunohistochemical expression of Cyclin D1 and Ki67 in salivary gland mucoepidermoid carcinomas T. Liu a,*, N. Xu a, E. Zhu a, L. Wang b a b
Dalian Medical University, China Northeastern University, China
Dalian Medical University, China Northeastern University, China
Introduction: The purpose of this study is to investigate the cytokeratin (CK) profile in salivary gland acinic cell carcinoma (ACC), then propose a rational hypothesis for its histogenesis. Methods: Tumor specimens from 18 ACC patients were obtained. The tissue specimens had been fixed in neutral-buffered formalin
Poster Abstracts Oral AbstractsPoster ListOrals ListPan. Disc. & Symp. Abs.Keynote Abs.Keynote Bios.ProgramIAOOWelcomeCommittee Listings
Oral Oncology Supplement 3 (2009) 123–161